Job ID = 14521527 SRX = SRX8499424 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 19723242 spots for SRR11955174/SRR11955174.sra Written 19723242 spots for SRR11955174/SRR11955174.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:18:49 19723242 reads; of these: 19723242 (100.00%) were paired; of these: 5826160 (29.54%) aligned concordantly 0 times 11806805 (59.86%) aligned concordantly exactly 1 time 2090277 (10.60%) aligned concordantly >1 times ---- 5826160 pairs aligned concordantly 0 times; of these: 2802917 (48.11%) aligned discordantly 1 time ---- 3023243 pairs aligned 0 times concordantly or discordantly; of these: 6046486 mates make up the pairs; of these: 4616539 (76.35%) aligned 0 times 404103 (6.68%) aligned exactly 1 time 1025844 (16.97%) aligned >1 times 88.30% overall alignment rate Time searching: 00:18:49 Overall time: 00:18:49 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 16 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 2315047 / 16515040 = 0.1402 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 21:37:54: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8499424/SRX8499424.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8499424/SRX8499424.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8499424/SRX8499424.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8499424/SRX8499424.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 21:37:54: #1 read tag files... INFO @ Sat, 15 Jan 2022 21:37:54: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 21:38:00: 1000000 INFO @ Sat, 15 Jan 2022 21:38:07: 2000000 INFO @ Sat, 15 Jan 2022 21:38:14: 3000000 INFO @ Sat, 15 Jan 2022 21:38:20: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 21:38:24: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8499424/SRX8499424.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8499424/SRX8499424.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8499424/SRX8499424.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8499424/SRX8499424.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 21:38:24: #1 read tag files... INFO @ Sat, 15 Jan 2022 21:38:24: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 21:38:27: 5000000 INFO @ Sat, 15 Jan 2022 21:38:31: 1000000 INFO @ Sat, 15 Jan 2022 21:38:35: 6000000 INFO @ Sat, 15 Jan 2022 21:38:38: 2000000 INFO @ Sat, 15 Jan 2022 21:38:42: 7000000 INFO @ Sat, 15 Jan 2022 21:38:45: 3000000 INFO @ Sat, 15 Jan 2022 21:38:49: 8000000 INFO @ Sat, 15 Jan 2022 21:38:52: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 21:38:54: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8499424/SRX8499424.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8499424/SRX8499424.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8499424/SRX8499424.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8499424/SRX8499424.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 21:38:54: #1 read tag files... INFO @ Sat, 15 Jan 2022 21:38:54: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 21:38:56: 9000000 INFO @ Sat, 15 Jan 2022 21:38:59: 5000000 INFO @ Sat, 15 Jan 2022 21:39:02: 1000000 INFO @ Sat, 15 Jan 2022 21:39:03: 10000000 INFO @ Sat, 15 Jan 2022 21:39:06: 6000000 INFO @ Sat, 15 Jan 2022 21:39:10: 2000000 INFO @ Sat, 15 Jan 2022 21:39:10: 11000000 INFO @ Sat, 15 Jan 2022 21:39:15: 7000000 INFO @ Sat, 15 Jan 2022 21:39:17: 12000000 INFO @ Sat, 15 Jan 2022 21:39:18: 3000000 INFO @ Sat, 15 Jan 2022 21:39:23: 8000000 INFO @ Sat, 15 Jan 2022 21:39:23: 13000000 INFO @ Sat, 15 Jan 2022 21:39:26: 4000000 INFO @ Sat, 15 Jan 2022 21:39:30: 14000000 INFO @ Sat, 15 Jan 2022 21:39:31: 9000000 INFO @ Sat, 15 Jan 2022 21:39:34: 5000000 INFO @ Sat, 15 Jan 2022 21:39:37: 15000000 INFO @ Sat, 15 Jan 2022 21:39:38: 10000000 INFO @ Sat, 15 Jan 2022 21:39:41: 6000000 INFO @ Sat, 15 Jan 2022 21:39:44: 16000000 INFO @ Sat, 15 Jan 2022 21:39:47: 11000000 INFO @ Sat, 15 Jan 2022 21:39:48: 7000000 INFO @ Sat, 15 Jan 2022 21:39:51: 17000000 INFO @ Sat, 15 Jan 2022 21:39:55: 8000000 INFO @ Sat, 15 Jan 2022 21:39:55: 12000000 INFO @ Sat, 15 Jan 2022 21:39:58: 18000000 INFO @ Sat, 15 Jan 2022 21:40:02: 9000000 INFO @ Sat, 15 Jan 2022 21:40:03: 13000000 INFO @ Sat, 15 Jan 2022 21:40:04: 19000000 INFO @ Sat, 15 Jan 2022 21:40:09: 10000000 INFO @ Sat, 15 Jan 2022 21:40:11: 20000000 INFO @ Sat, 15 Jan 2022 21:40:11: 14000000 INFO @ Sat, 15 Jan 2022 21:40:16: 11000000 INFO @ Sat, 15 Jan 2022 21:40:18: 21000000 INFO @ Sat, 15 Jan 2022 21:40:20: 15000000 INFO @ Sat, 15 Jan 2022 21:40:22: 12000000 INFO @ Sat, 15 Jan 2022 21:40:25: 22000000 INFO @ Sat, 15 Jan 2022 21:40:28: 16000000 INFO @ Sat, 15 Jan 2022 21:40:29: 13000000 INFO @ Sat, 15 Jan 2022 21:40:32: 23000000 INFO @ Sat, 15 Jan 2022 21:40:36: 17000000 INFO @ Sat, 15 Jan 2022 21:40:36: 14000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 21:40:39: 24000000 INFO @ Sat, 15 Jan 2022 21:40:43: 15000000 INFO @ Sat, 15 Jan 2022 21:40:44: 18000000 INFO @ Sat, 15 Jan 2022 21:40:45: 25000000 INFO @ Sat, 15 Jan 2022 21:40:50: 16000000 BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 21:40:52: 26000000 INFO @ Sat, 15 Jan 2022 21:40:52: 19000000 INFO @ Sat, 15 Jan 2022 21:40:57: 17000000 INFO @ Sat, 15 Jan 2022 21:40:59: 27000000 INFO @ Sat, 15 Jan 2022 21:41:01: 20000000 INFO @ Sat, 15 Jan 2022 21:41:04: 18000000 INFO @ Sat, 15 Jan 2022 21:41:06: 28000000 INFO @ Sat, 15 Jan 2022 21:41:09: 21000000 INFO @ Sat, 15 Jan 2022 21:41:11: 19000000 INFO @ Sat, 15 Jan 2022 21:41:13: 29000000 INFO @ Sat, 15 Jan 2022 21:41:17: 22000000 INFO @ Sat, 15 Jan 2022 21:41:18: 20000000 INFO @ Sat, 15 Jan 2022 21:41:19: 30000000 INFO @ Sat, 15 Jan 2022 21:41:21: #1 tag size is determined as 101 bps INFO @ Sat, 15 Jan 2022 21:41:21: #1 tag size = 101 INFO @ Sat, 15 Jan 2022 21:41:21: #1 total tags in treatment: 11949613 INFO @ Sat, 15 Jan 2022 21:41:21: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 21:41:21: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 21:41:21: #1 tags after filtering in treatment: 8556619 INFO @ Sat, 15 Jan 2022 21:41:21: #1 Redundant rate of treatment: 0.28 INFO @ Sat, 15 Jan 2022 21:41:21: #1 finished! INFO @ Sat, 15 Jan 2022 21:41:21: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 21:41:21: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 21:41:21: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 21:41:21: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 21:41:21: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8499424/SRX8499424.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8499424/SRX8499424.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8499424/SRX8499424.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8499424/SRX8499424.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 21:41:24: 21000000 INFO @ Sat, 15 Jan 2022 21:41:25: 23000000 INFO @ Sat, 15 Jan 2022 21:41:31: 22000000 INFO @ Sat, 15 Jan 2022 21:41:33: 24000000 INFO @ Sat, 15 Jan 2022 21:41:38: 23000000 INFO @ Sat, 15 Jan 2022 21:41:42: 25000000 INFO @ Sat, 15 Jan 2022 21:41:45: 24000000 INFO @ Sat, 15 Jan 2022 21:41:50: 26000000 INFO @ Sat, 15 Jan 2022 21:41:52: 25000000 INFO @ Sat, 15 Jan 2022 21:41:58: 27000000 INFO @ Sat, 15 Jan 2022 21:41:59: 26000000 INFO @ Sat, 15 Jan 2022 21:42:05: 27000000 INFO @ Sat, 15 Jan 2022 21:42:06: 28000000 INFO @ Sat, 15 Jan 2022 21:42:12: 28000000 INFO @ Sat, 15 Jan 2022 21:42:14: 29000000 INFO @ Sat, 15 Jan 2022 21:42:19: 29000000 INFO @ Sat, 15 Jan 2022 21:42:23: 30000000 INFO @ Sat, 15 Jan 2022 21:42:24: #1 tag size is determined as 101 bps INFO @ Sat, 15 Jan 2022 21:42:24: #1 tag size = 101 INFO @ Sat, 15 Jan 2022 21:42:24: #1 total tags in treatment: 11949613 INFO @ Sat, 15 Jan 2022 21:42:24: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 21:42:24: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 21:42:24: #1 tags after filtering in treatment: 8556619 INFO @ Sat, 15 Jan 2022 21:42:24: #1 Redundant rate of treatment: 0.28 INFO @ Sat, 15 Jan 2022 21:42:24: #1 finished! INFO @ Sat, 15 Jan 2022 21:42:24: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 21:42:24: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 21:42:25: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 21:42:25: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 21:42:25: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8499424/SRX8499424.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8499424/SRX8499424.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8499424/SRX8499424.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8499424/SRX8499424.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 21:42:26: 30000000 INFO @ Sat, 15 Jan 2022 21:42:27: #1 tag size is determined as 101 bps INFO @ Sat, 15 Jan 2022 21:42:27: #1 tag size = 101 INFO @ Sat, 15 Jan 2022 21:42:27: #1 total tags in treatment: 11949613 INFO @ Sat, 15 Jan 2022 21:42:27: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 21:42:27: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 21:42:27: #1 tags after filtering in treatment: 8556619 INFO @ Sat, 15 Jan 2022 21:42:27: #1 Redundant rate of treatment: 0.28 INFO @ Sat, 15 Jan 2022 21:42:27: #1 finished! INFO @ Sat, 15 Jan 2022 21:42:27: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 21:42:27: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 21:42:28: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 21:42:28: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 21:42:28: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8499424/SRX8499424.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8499424/SRX8499424.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8499424/SRX8499424.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8499424/SRX8499424.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling