Job ID = 14521526 SRX = SRX8499423 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 20845387 spots for SRR11955173/SRR11955173.sra Written 20845387 spots for SRR11955173/SRR11955173.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:27:21 20845387 reads; of these: 20845387 (100.00%) were paired; of these: 6375656 (30.59%) aligned concordantly 0 times 12262692 (58.83%) aligned concordantly exactly 1 time 2207039 (10.59%) aligned concordantly >1 times ---- 6375656 pairs aligned concordantly 0 times; of these: 2602332 (40.82%) aligned discordantly 1 time ---- 3773324 pairs aligned 0 times concordantly or discordantly; of these: 7546648 mates make up the pairs; of these: 6180957 (81.90%) aligned 0 times 372818 (4.94%) aligned exactly 1 time 992873 (13.16%) aligned >1 times 85.17% overall alignment rate Time searching: 00:27:21 Overall time: 00:27:21 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 16 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 2071570 / 16940805 = 0.1223 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 21:49:03: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8499423/SRX8499423.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8499423/SRX8499423.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8499423/SRX8499423.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8499423/SRX8499423.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 21:49:03: #1 read tag files... INFO @ Sat, 15 Jan 2022 21:49:03: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 21:49:09: 1000000 INFO @ Sat, 15 Jan 2022 21:49:15: 2000000 INFO @ Sat, 15 Jan 2022 21:49:22: 3000000 INFO @ Sat, 15 Jan 2022 21:49:28: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 21:49:33: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8499423/SRX8499423.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8499423/SRX8499423.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8499423/SRX8499423.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8499423/SRX8499423.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 21:49:33: #1 read tag files... INFO @ Sat, 15 Jan 2022 21:49:33: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 21:49:34: 5000000 INFO @ Sat, 15 Jan 2022 21:49:40: 1000000 INFO @ Sat, 15 Jan 2022 21:49:41: 6000000 INFO @ Sat, 15 Jan 2022 21:49:47: 2000000 INFO @ Sat, 15 Jan 2022 21:49:48: 7000000 INFO @ Sat, 15 Jan 2022 21:49:54: 3000000 INFO @ Sat, 15 Jan 2022 21:49:55: 8000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 21:50:01: 4000000 INFO @ Sat, 15 Jan 2022 21:50:02: 9000000 INFO @ Sat, 15 Jan 2022 21:50:03: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8499423/SRX8499423.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8499423/SRX8499423.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8499423/SRX8499423.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8499423/SRX8499423.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 21:50:03: #1 read tag files... INFO @ Sat, 15 Jan 2022 21:50:03: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 21:50:08: 5000000 INFO @ Sat, 15 Jan 2022 21:50:09: 10000000 INFO @ Sat, 15 Jan 2022 21:50:12: 1000000 INFO @ Sat, 15 Jan 2022 21:50:15: 6000000 INFO @ Sat, 15 Jan 2022 21:50:16: 11000000 INFO @ Sat, 15 Jan 2022 21:50:21: 2000000 INFO @ Sat, 15 Jan 2022 21:50:22: 7000000 INFO @ Sat, 15 Jan 2022 21:50:23: 12000000 INFO @ Sat, 15 Jan 2022 21:50:30: 13000000 INFO @ Sat, 15 Jan 2022 21:50:30: 8000000 INFO @ Sat, 15 Jan 2022 21:50:31: 3000000 INFO @ Sat, 15 Jan 2022 21:50:37: 14000000 INFO @ Sat, 15 Jan 2022 21:50:37: 9000000 INFO @ Sat, 15 Jan 2022 21:50:41: 4000000 INFO @ Sat, 15 Jan 2022 21:50:44: 15000000 INFO @ Sat, 15 Jan 2022 21:50:44: 10000000 INFO @ Sat, 15 Jan 2022 21:50:50: 5000000 INFO @ Sat, 15 Jan 2022 21:50:51: 16000000 INFO @ Sat, 15 Jan 2022 21:50:52: 11000000 INFO @ Sat, 15 Jan 2022 21:50:59: 17000000 INFO @ Sat, 15 Jan 2022 21:50:59: 6000000 INFO @ Sat, 15 Jan 2022 21:51:00: 12000000 INFO @ Sat, 15 Jan 2022 21:51:06: 18000000 INFO @ Sat, 15 Jan 2022 21:51:07: 13000000 INFO @ Sat, 15 Jan 2022 21:51:08: 7000000 INFO @ Sat, 15 Jan 2022 21:51:14: 19000000 INFO @ Sat, 15 Jan 2022 21:51:14: 14000000 INFO @ Sat, 15 Jan 2022 21:51:17: 8000000 INFO @ Sat, 15 Jan 2022 21:51:21: 20000000 INFO @ Sat, 15 Jan 2022 21:51:22: 15000000 INFO @ Sat, 15 Jan 2022 21:51:26: 9000000 INFO @ Sat, 15 Jan 2022 21:51:28: 21000000 INFO @ Sat, 15 Jan 2022 21:51:29: 16000000 INFO @ Sat, 15 Jan 2022 21:51:35: 10000000 INFO @ Sat, 15 Jan 2022 21:51:36: 22000000 INFO @ Sat, 15 Jan 2022 21:51:37: 17000000 INFO @ Sat, 15 Jan 2022 21:51:43: 23000000 INFO @ Sat, 15 Jan 2022 21:51:44: 18000000 INFO @ Sat, 15 Jan 2022 21:51:44: 11000000 INFO @ Sat, 15 Jan 2022 21:51:51: 24000000 INFO @ Sat, 15 Jan 2022 21:51:52: 19000000 INFO @ Sat, 15 Jan 2022 21:51:53: 12000000 INFO @ Sat, 15 Jan 2022 21:51:58: 25000000 INFO @ Sat, 15 Jan 2022 21:51:59: 20000000 INFO @ Sat, 15 Jan 2022 21:52:02: 13000000 INFO @ Sat, 15 Jan 2022 21:52:05: 26000000 INFO @ Sat, 15 Jan 2022 21:52:06: 21000000 INFO @ Sat, 15 Jan 2022 21:52:11: 14000000 INFO @ Sat, 15 Jan 2022 21:52:13: 27000000 INFO @ Sat, 15 Jan 2022 21:52:13: 22000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 21:52:20: 28000000 INFO @ Sat, 15 Jan 2022 21:52:21: 15000000 INFO @ Sat, 15 Jan 2022 21:52:21: 23000000 INFO @ Sat, 15 Jan 2022 21:52:27: 29000000 INFO @ Sat, 15 Jan 2022 21:52:28: 24000000 INFO @ Sat, 15 Jan 2022 21:52:30: 16000000 INFO @ Sat, 15 Jan 2022 21:52:35: 30000000 INFO @ Sat, 15 Jan 2022 21:52:35: 25000000 INFO @ Sat, 15 Jan 2022 21:52:39: 17000000 BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 21:52:42: 31000000 INFO @ Sat, 15 Jan 2022 21:52:43: 26000000 INFO @ Sat, 15 Jan 2022 21:52:45: #1 tag size is determined as 101 bps INFO @ Sat, 15 Jan 2022 21:52:45: #1 tag size = 101 INFO @ Sat, 15 Jan 2022 21:52:45: #1 total tags in treatment: 12702209 INFO @ Sat, 15 Jan 2022 21:52:45: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 21:52:45: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 21:52:45: #1 tags after filtering in treatment: 8882904 INFO @ Sat, 15 Jan 2022 21:52:45: #1 Redundant rate of treatment: 0.30 INFO @ Sat, 15 Jan 2022 21:52:45: #1 finished! INFO @ Sat, 15 Jan 2022 21:52:45: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 21:52:45: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 21:52:46: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 21:52:46: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 21:52:46: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8499423/SRX8499423.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8499423/SRX8499423.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8499423/SRX8499423.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8499423/SRX8499423.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 21:52:48: 18000000 INFO @ Sat, 15 Jan 2022 21:52:50: 27000000 INFO @ Sat, 15 Jan 2022 21:52:56: 28000000 INFO @ Sat, 15 Jan 2022 21:52:57: 19000000 INFO @ Sat, 15 Jan 2022 21:53:03: 29000000 INFO @ Sat, 15 Jan 2022 21:53:06: 20000000 INFO @ Sat, 15 Jan 2022 21:53:10: 30000000 INFO @ Sat, 15 Jan 2022 21:53:15: 21000000 INFO @ Sat, 15 Jan 2022 21:53:16: 31000000 INFO @ Sat, 15 Jan 2022 21:53:19: #1 tag size is determined as 101 bps INFO @ Sat, 15 Jan 2022 21:53:19: #1 tag size = 101 INFO @ Sat, 15 Jan 2022 21:53:19: #1 total tags in treatment: 12702209 INFO @ Sat, 15 Jan 2022 21:53:19: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 21:53:19: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 21:53:19: #1 tags after filtering in treatment: 8882904 INFO @ Sat, 15 Jan 2022 21:53:19: #1 Redundant rate of treatment: 0.30 INFO @ Sat, 15 Jan 2022 21:53:19: #1 finished! INFO @ Sat, 15 Jan 2022 21:53:19: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 21:53:19: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 21:53:20: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 21:53:20: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 21:53:20: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8499423/SRX8499423.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8499423/SRX8499423.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8499423/SRX8499423.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8499423/SRX8499423.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 21:53:24: 22000000 INFO @ Sat, 15 Jan 2022 21:53:33: 23000000 INFO @ Sat, 15 Jan 2022 21:53:41: 24000000 INFO @ Sat, 15 Jan 2022 21:53:50: 25000000 INFO @ Sat, 15 Jan 2022 21:53:59: 26000000 INFO @ Sat, 15 Jan 2022 21:54:08: 27000000 INFO @ Sat, 15 Jan 2022 21:54:16: 28000000 INFO @ Sat, 15 Jan 2022 21:54:25: 29000000 INFO @ Sat, 15 Jan 2022 21:54:34: 30000000 INFO @ Sat, 15 Jan 2022 21:54:42: 31000000 INFO @ Sat, 15 Jan 2022 21:54:45: #1 tag size is determined as 101 bps INFO @ Sat, 15 Jan 2022 21:54:45: #1 tag size = 101 INFO @ Sat, 15 Jan 2022 21:54:45: #1 total tags in treatment: 12702209 INFO @ Sat, 15 Jan 2022 21:54:45: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 21:54:45: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 21:54:46: #1 tags after filtering in treatment: 8882904 INFO @ Sat, 15 Jan 2022 21:54:46: #1 Redundant rate of treatment: 0.30 INFO @ Sat, 15 Jan 2022 21:54:46: #1 finished! INFO @ Sat, 15 Jan 2022 21:54:46: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 21:54:46: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 21:54:46: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 21:54:46: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 21:54:46: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8499423/SRX8499423.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8499423/SRX8499423.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8499423/SRX8499423.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8499423/SRX8499423.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling