Job ID = 14521525 SRX = SRX8499422 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 22813816 spots for SRR11955172/SRR11955172.sra Written 22813816 spots for SRR11955172/SRR11955172.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:24:40 22813816 reads; of these: 22813816 (100.00%) were paired; of these: 6405440 (28.08%) aligned concordantly 0 times 13822668 (60.59%) aligned concordantly exactly 1 time 2585708 (11.33%) aligned concordantly >1 times ---- 6405440 pairs aligned concordantly 0 times; of these: 2447957 (38.22%) aligned discordantly 1 time ---- 3957483 pairs aligned 0 times concordantly or discordantly; of these: 7914966 mates make up the pairs; of these: 6521848 (82.40%) aligned 0 times 427425 (5.40%) aligned exactly 1 time 965693 (12.20%) aligned >1 times 85.71% overall alignment rate Time searching: 00:24:40 Overall time: 00:24:40 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 16 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 2863198 / 18691634 = 0.1532 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 21:43:26: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8499422/SRX8499422.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8499422/SRX8499422.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8499422/SRX8499422.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8499422/SRX8499422.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 21:43:26: #1 read tag files... INFO @ Sat, 15 Jan 2022 21:43:26: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 21:43:34: 1000000 INFO @ Sat, 15 Jan 2022 21:43:43: 2000000 INFO @ Sat, 15 Jan 2022 21:43:51: 3000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 21:43:56: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8499422/SRX8499422.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8499422/SRX8499422.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8499422/SRX8499422.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8499422/SRX8499422.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 21:43:56: #1 read tag files... INFO @ Sat, 15 Jan 2022 21:43:56: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 21:44:00: 4000000 INFO @ Sat, 15 Jan 2022 21:44:05: 1000000 INFO @ Sat, 15 Jan 2022 21:44:09: 5000000 INFO @ Sat, 15 Jan 2022 21:44:13: 2000000 INFO @ Sat, 15 Jan 2022 21:44:18: 6000000 INFO @ Sat, 15 Jan 2022 21:44:21: 3000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 21:44:26: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8499422/SRX8499422.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8499422/SRX8499422.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8499422/SRX8499422.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8499422/SRX8499422.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 21:44:26: #1 read tag files... INFO @ Sat, 15 Jan 2022 21:44:26: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 21:44:28: 7000000 INFO @ Sat, 15 Jan 2022 21:44:29: 4000000 INFO @ Sat, 15 Jan 2022 21:44:36: 1000000 INFO @ Sat, 15 Jan 2022 21:44:37: 8000000 INFO @ Sat, 15 Jan 2022 21:44:37: 5000000 INFO @ Sat, 15 Jan 2022 21:44:45: 2000000 INFO @ Sat, 15 Jan 2022 21:44:46: 6000000 INFO @ Sat, 15 Jan 2022 21:44:47: 9000000 INFO @ Sat, 15 Jan 2022 21:44:54: 7000000 INFO @ Sat, 15 Jan 2022 21:44:55: 3000000 INFO @ Sat, 15 Jan 2022 21:44:56: 10000000 INFO @ Sat, 15 Jan 2022 21:45:02: 8000000 INFO @ Sat, 15 Jan 2022 21:45:04: 4000000 INFO @ Sat, 15 Jan 2022 21:45:06: 11000000 INFO @ Sat, 15 Jan 2022 21:45:11: 9000000 INFO @ Sat, 15 Jan 2022 21:45:14: 5000000 INFO @ Sat, 15 Jan 2022 21:45:15: 12000000 INFO @ Sat, 15 Jan 2022 21:45:19: 10000000 INFO @ Sat, 15 Jan 2022 21:45:24: 6000000 INFO @ Sat, 15 Jan 2022 21:45:25: 13000000 INFO @ Sat, 15 Jan 2022 21:45:27: 11000000 INFO @ Sat, 15 Jan 2022 21:45:33: 7000000 INFO @ Sat, 15 Jan 2022 21:45:34: 14000000 INFO @ Sat, 15 Jan 2022 21:45:36: 12000000 INFO @ Sat, 15 Jan 2022 21:45:43: 8000000 INFO @ Sat, 15 Jan 2022 21:45:44: 15000000 INFO @ Sat, 15 Jan 2022 21:45:44: 13000000 INFO @ Sat, 15 Jan 2022 21:45:52: 14000000 INFO @ Sat, 15 Jan 2022 21:45:53: 9000000 INFO @ Sat, 15 Jan 2022 21:45:53: 16000000 INFO @ Sat, 15 Jan 2022 21:46:01: 15000000 INFO @ Sat, 15 Jan 2022 21:46:02: 10000000 INFO @ Sat, 15 Jan 2022 21:46:03: 17000000 INFO @ Sat, 15 Jan 2022 21:46:09: 16000000 INFO @ Sat, 15 Jan 2022 21:46:12: 11000000 INFO @ Sat, 15 Jan 2022 21:46:12: 18000000 INFO @ Sat, 15 Jan 2022 21:46:18: 17000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 21:46:22: 19000000 INFO @ Sat, 15 Jan 2022 21:46:22: 12000000 INFO @ Sat, 15 Jan 2022 21:46:26: 18000000 INFO @ Sat, 15 Jan 2022 21:46:32: 20000000 INFO @ Sat, 15 Jan 2022 21:46:32: 13000000 BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 21:46:35: 19000000 INFO @ Sat, 15 Jan 2022 21:46:41: 21000000 INFO @ Sat, 15 Jan 2022 21:46:42: 14000000 INFO @ Sat, 15 Jan 2022 21:46:43: 20000000 INFO @ Sat, 15 Jan 2022 21:46:51: 22000000 INFO @ Sat, 15 Jan 2022 21:46:51: 21000000 INFO @ Sat, 15 Jan 2022 21:46:52: 15000000 INFO @ Sat, 15 Jan 2022 21:47:00: 22000000 INFO @ Sat, 15 Jan 2022 21:47:00: 23000000 INFO @ Sat, 15 Jan 2022 21:47:02: 16000000 INFO @ Sat, 15 Jan 2022 21:47:08: 23000000 INFO @ Sat, 15 Jan 2022 21:47:09: 24000000 INFO @ Sat, 15 Jan 2022 21:47:11: 17000000 INFO @ Sat, 15 Jan 2022 21:47:16: 24000000 INFO @ Sat, 15 Jan 2022 21:47:19: 25000000 INFO @ Sat, 15 Jan 2022 21:47:21: 18000000 INFO @ Sat, 15 Jan 2022 21:47:25: 25000000 INFO @ Sat, 15 Jan 2022 21:47:28: 26000000 INFO @ Sat, 15 Jan 2022 21:47:31: 19000000 INFO @ Sat, 15 Jan 2022 21:47:33: 26000000 INFO @ Sat, 15 Jan 2022 21:47:37: 27000000 INFO @ Sat, 15 Jan 2022 21:47:40: 20000000 INFO @ Sat, 15 Jan 2022 21:47:41: 27000000 INFO @ Sat, 15 Jan 2022 21:47:47: 28000000 INFO @ Sat, 15 Jan 2022 21:47:50: 28000000 INFO @ Sat, 15 Jan 2022 21:47:50: 21000000 INFO @ Sat, 15 Jan 2022 21:47:56: 29000000 INFO @ Sat, 15 Jan 2022 21:47:59: 29000000 INFO @ Sat, 15 Jan 2022 21:48:00: 22000000 INFO @ Sat, 15 Jan 2022 21:48:06: 30000000 INFO @ Sat, 15 Jan 2022 21:48:08: 30000000 INFO @ Sat, 15 Jan 2022 21:48:09: 23000000 INFO @ Sat, 15 Jan 2022 21:48:16: 31000000 INFO @ Sat, 15 Jan 2022 21:48:17: 31000000 INFO @ Sat, 15 Jan 2022 21:48:18: 24000000 INFO @ Sat, 15 Jan 2022 21:48:26: 32000000 INFO @ Sat, 15 Jan 2022 21:48:27: 32000000 INFO @ Sat, 15 Jan 2022 21:48:27: 25000000 INFO @ Sat, 15 Jan 2022 21:48:35: 33000000 INFO @ Sat, 15 Jan 2022 21:48:37: 26000000 INFO @ Sat, 15 Jan 2022 21:48:37: 33000000 INFO @ Sat, 15 Jan 2022 21:48:39: #1 tag size is determined as 101 bps INFO @ Sat, 15 Jan 2022 21:48:39: #1 tag size = 101 INFO @ Sat, 15 Jan 2022 21:48:39: #1 total tags in treatment: 13881696 INFO @ Sat, 15 Jan 2022 21:48:39: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 21:48:39: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 21:48:39: #1 tags after filtering in treatment: 9533871 INFO @ Sat, 15 Jan 2022 21:48:39: #1 Redundant rate of treatment: 0.31 INFO @ Sat, 15 Jan 2022 21:48:39: #1 finished! INFO @ Sat, 15 Jan 2022 21:48:39: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 21:48:39: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 21:48:40: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 21:48:40: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 21:48:40: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8499422/SRX8499422.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8499422/SRX8499422.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8499422/SRX8499422.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8499422/SRX8499422.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 21:48:40: #1 tag size is determined as 101 bps INFO @ Sat, 15 Jan 2022 21:48:40: #1 tag size = 101 INFO @ Sat, 15 Jan 2022 21:48:40: #1 total tags in treatment: 13881696 INFO @ Sat, 15 Jan 2022 21:48:40: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 21:48:40: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 21:48:40: #1 tags after filtering in treatment: 9533871 INFO @ Sat, 15 Jan 2022 21:48:40: #1 Redundant rate of treatment: 0.31 INFO @ Sat, 15 Jan 2022 21:48:40: #1 finished! INFO @ Sat, 15 Jan 2022 21:48:40: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 21:48:40: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 21:48:41: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 21:48:41: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 21:48:41: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8499422/SRX8499422.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8499422/SRX8499422.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8499422/SRX8499422.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8499422/SRX8499422.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 21:48:45: 27000000 INFO @ Sat, 15 Jan 2022 21:48:53: 28000000 INFO @ Sat, 15 Jan 2022 21:49:02: 29000000 INFO @ Sat, 15 Jan 2022 21:49:11: 30000000 INFO @ Sat, 15 Jan 2022 21:49:19: 31000000 INFO @ Sat, 15 Jan 2022 21:49:28: 32000000 INFO @ Sat, 15 Jan 2022 21:49:36: 33000000 INFO @ Sat, 15 Jan 2022 21:49:39: #1 tag size is determined as 101 bps INFO @ Sat, 15 Jan 2022 21:49:39: #1 tag size = 101 INFO @ Sat, 15 Jan 2022 21:49:39: #1 total tags in treatment: 13881696 INFO @ Sat, 15 Jan 2022 21:49:39: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 21:49:39: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 21:49:39: #1 tags after filtering in treatment: 9533871 INFO @ Sat, 15 Jan 2022 21:49:39: #1 Redundant rate of treatment: 0.31 INFO @ Sat, 15 Jan 2022 21:49:39: #1 finished! INFO @ Sat, 15 Jan 2022 21:49:39: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 21:49:39: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 21:49:40: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 21:49:40: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 21:49:40: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8499422/SRX8499422.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8499422/SRX8499422.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8499422/SRX8499422.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8499422/SRX8499422.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling