Job ID = 7115012 SRX = SRX8462220 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... 2020-07-22T06:06:04 prefetch.2.10.7: 1) Downloading 'SRR11915679'... 2020-07-22T06:06:04 prefetch.2.10.7: Downloading via HTTPS... 2020-07-22T06:06:33 prefetch.2.10.7: HTTPS download succeed 2020-07-22T06:06:34 prefetch.2.10.7: 'SRR11915679' is valid 2020-07-22T06:06:34 prefetch.2.10.7: 1) 'SRR11915679' was downloaded successfully 2020-07-22T06:06:34 prefetch.2.10.7: 'SRR11915679' has 0 unresolved dependencies Read 2490909 spots for SRR11915679/SRR11915679.sra Written 2490909 spots for SRR11915679/SRR11915679.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:02:22 2490909 reads; of these: 2490909 (100.00%) were paired; of these: 133070 (5.34%) aligned concordantly 0 times 2049898 (82.30%) aligned concordantly exactly 1 time 307941 (12.36%) aligned concordantly >1 times ---- 133070 pairs aligned concordantly 0 times; of these: 14812 (11.13%) aligned discordantly 1 time ---- 118258 pairs aligned 0 times concordantly or discordantly; of these: 236516 mates make up the pairs; of these: 180721 (76.41%) aligned 0 times 43792 (18.52%) aligned exactly 1 time 12003 (5.07%) aligned >1 times 96.37% overall alignment rate Time searching: 00:02:22 Overall time: 00:02:22 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 306786 / 2367781 = 0.1296 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 22 Jul 2020 15:11:15: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8462220/SRX8462220.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8462220/SRX8462220.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8462220/SRX8462220.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8462220/SRX8462220.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 22 Jul 2020 15:11:15: #1 read tag files... INFO @ Wed, 22 Jul 2020 15:11:15: #1 read treatment tags... INFO @ Wed, 22 Jul 2020 15:11:21: 1000000 INFO @ Wed, 22 Jul 2020 15:11:26: 2000000 INFO @ Wed, 22 Jul 2020 15:11:32: 3000000 INFO @ Wed, 22 Jul 2020 15:11:37: 4000000 INFO @ Wed, 22 Jul 2020 15:11:38: #1 tag size is determined as 75 bps INFO @ Wed, 22 Jul 2020 15:11:38: #1 tag size = 75 INFO @ Wed, 22 Jul 2020 15:11:38: #1 total tags in treatment: 2051631 INFO @ Wed, 22 Jul 2020 15:11:38: #1 user defined the maximum tags... INFO @ Wed, 22 Jul 2020 15:11:38: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 22 Jul 2020 15:11:38: #1 tags after filtering in treatment: 1810371 INFO @ Wed, 22 Jul 2020 15:11:38: #1 Redundant rate of treatment: 0.12 INFO @ Wed, 22 Jul 2020 15:11:38: #1 finished! INFO @ Wed, 22 Jul 2020 15:11:38: #2 Build Peak Model... INFO @ Wed, 22 Jul 2020 15:11:38: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 22 Jul 2020 15:11:38: #2 number of paired peaks: 96 WARNING @ Wed, 22 Jul 2020 15:11:38: Too few paired peaks (96) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 22 Jul 2020 15:11:38: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8462220/SRX8462220.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8462220/SRX8462220.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8462220/SRX8462220.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8462220/SRX8462220.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 22 Jul 2020 15:11:45: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8462220/SRX8462220.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8462220/SRX8462220.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8462220/SRX8462220.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8462220/SRX8462220.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 22 Jul 2020 15:11:45: #1 read tag files... INFO @ Wed, 22 Jul 2020 15:11:45: #1 read treatment tags... INFO @ Wed, 22 Jul 2020 15:11:52: 1000000 INFO @ Wed, 22 Jul 2020 15:11:58: 2000000 INFO @ Wed, 22 Jul 2020 15:12:04: 3000000 INFO @ Wed, 22 Jul 2020 15:12:10: 4000000 INFO @ Wed, 22 Jul 2020 15:12:12: #1 tag size is determined as 75 bps INFO @ Wed, 22 Jul 2020 15:12:12: #1 tag size = 75 INFO @ Wed, 22 Jul 2020 15:12:12: #1 total tags in treatment: 2051631 INFO @ Wed, 22 Jul 2020 15:12:12: #1 user defined the maximum tags... INFO @ Wed, 22 Jul 2020 15:12:12: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 22 Jul 2020 15:12:12: #1 tags after filtering in treatment: 1810371 INFO @ Wed, 22 Jul 2020 15:12:12: #1 Redundant rate of treatment: 0.12 INFO @ Wed, 22 Jul 2020 15:12:12: #1 finished! INFO @ Wed, 22 Jul 2020 15:12:12: #2 Build Peak Model... INFO @ Wed, 22 Jul 2020 15:12:12: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 22 Jul 2020 15:12:12: #2 number of paired peaks: 96 WARNING @ Wed, 22 Jul 2020 15:12:12: Too few paired peaks (96) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 22 Jul 2020 15:12:12: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8462220/SRX8462220.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8462220/SRX8462220.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8462220/SRX8462220.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8462220/SRX8462220.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 22 Jul 2020 15:12:15: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8462220/SRX8462220.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8462220/SRX8462220.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8462220/SRX8462220.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8462220/SRX8462220.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 22 Jul 2020 15:12:15: #1 read tag files... INFO @ Wed, 22 Jul 2020 15:12:15: #1 read treatment tags... INFO @ Wed, 22 Jul 2020 15:12:22: 1000000 INFO @ Wed, 22 Jul 2020 15:12:28: 2000000 INFO @ Wed, 22 Jul 2020 15:12:34: 3000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Wed, 22 Jul 2020 15:12:40: 4000000 INFO @ Wed, 22 Jul 2020 15:12:41: #1 tag size is determined as 75 bps INFO @ Wed, 22 Jul 2020 15:12:41: #1 tag size = 75 INFO @ Wed, 22 Jul 2020 15:12:41: #1 total tags in treatment: 2051631 INFO @ Wed, 22 Jul 2020 15:12:41: #1 user defined the maximum tags... INFO @ Wed, 22 Jul 2020 15:12:41: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 22 Jul 2020 15:12:41: #1 tags after filtering in treatment: 1810371 INFO @ Wed, 22 Jul 2020 15:12:41: #1 Redundant rate of treatment: 0.12 INFO @ Wed, 22 Jul 2020 15:12:41: #1 finished! INFO @ Wed, 22 Jul 2020 15:12:41: #2 Build Peak Model... INFO @ Wed, 22 Jul 2020 15:12:41: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 22 Jul 2020 15:12:41: #2 number of paired peaks: 96 WARNING @ Wed, 22 Jul 2020 15:12:41: Too few paired peaks (96) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 22 Jul 2020 15:12:41: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8462220/SRX8462220.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8462220/SRX8462220.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8462220/SRX8462220.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8462220/SRX8462220.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。