Job ID = 7114709 SRX = SRX8462210 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... 2020-07-22T06:01:18 prefetch.2.10.7: 1) Downloading 'SRR11915669'... 2020-07-22T06:01:18 prefetch.2.10.7: Downloading via HTTPS... 2020-07-22T06:02:05 prefetch.2.10.7: HTTPS download succeed 2020-07-22T06:02:05 prefetch.2.10.7: 'SRR11915669' is valid 2020-07-22T06:02:05 prefetch.2.10.7: 1) 'SRR11915669' was downloaded successfully 2020-07-22T06:02:05 prefetch.2.10.7: 'SRR11915669' has 0 unresolved dependencies Read 2518441 spots for SRR11915669/SRR11915669.sra Written 2518441 spots for SRR11915669/SRR11915669.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:02:19 2518441 reads; of these: 2518441 (100.00%) were paired; of these: 174794 (6.94%) aligned concordantly 0 times 2027289 (80.50%) aligned concordantly exactly 1 time 316358 (12.56%) aligned concordantly >1 times ---- 174794 pairs aligned concordantly 0 times; of these: 12520 (7.16%) aligned discordantly 1 time ---- 162274 pairs aligned 0 times concordantly or discordantly; of these: 324548 mates make up the pairs; of these: 268444 (82.71%) aligned 0 times 43992 (13.55%) aligned exactly 1 time 12112 (3.73%) aligned >1 times 94.67% overall alignment rate Time searching: 00:02:19 Overall time: 00:02:19 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 335213 / 2351456 = 0.1426 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 22 Jul 2020 15:06:44: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8462210/SRX8462210.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8462210/SRX8462210.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8462210/SRX8462210.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8462210/SRX8462210.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 22 Jul 2020 15:06:44: #1 read tag files... INFO @ Wed, 22 Jul 2020 15:06:44: #1 read treatment tags... INFO @ Wed, 22 Jul 2020 15:06:51: 1000000 INFO @ Wed, 22 Jul 2020 15:06:58: 2000000 INFO @ Wed, 22 Jul 2020 15:07:04: 3000000 INFO @ Wed, 22 Jul 2020 15:07:11: 4000000 INFO @ Wed, 22 Jul 2020 15:07:12: #1 tag size is determined as 75 bps INFO @ Wed, 22 Jul 2020 15:07:12: #1 tag size = 75 INFO @ Wed, 22 Jul 2020 15:07:12: #1 total tags in treatment: 2008920 INFO @ Wed, 22 Jul 2020 15:07:12: #1 user defined the maximum tags... INFO @ Wed, 22 Jul 2020 15:07:12: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 22 Jul 2020 15:07:12: #1 tags after filtering in treatment: 1767955 INFO @ Wed, 22 Jul 2020 15:07:12: #1 Redundant rate of treatment: 0.12 INFO @ Wed, 22 Jul 2020 15:07:12: #1 finished! INFO @ Wed, 22 Jul 2020 15:07:12: #2 Build Peak Model... INFO @ Wed, 22 Jul 2020 15:07:12: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 22 Jul 2020 15:07:12: #2 number of paired peaks: 73 WARNING @ Wed, 22 Jul 2020 15:07:12: Too few paired peaks (73) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 22 Jul 2020 15:07:12: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8462210/SRX8462210.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8462210/SRX8462210.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8462210/SRX8462210.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8462210/SRX8462210.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 22 Jul 2020 15:07:14: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8462210/SRX8462210.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8462210/SRX8462210.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8462210/SRX8462210.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8462210/SRX8462210.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 22 Jul 2020 15:07:14: #1 read tag files... INFO @ Wed, 22 Jul 2020 15:07:14: #1 read treatment tags... INFO @ Wed, 22 Jul 2020 15:07:21: 1000000 INFO @ Wed, 22 Jul 2020 15:07:28: 2000000 INFO @ Wed, 22 Jul 2020 15:07:34: 3000000 INFO @ Wed, 22 Jul 2020 15:07:41: 4000000 INFO @ Wed, 22 Jul 2020 15:07:41: #1 tag size is determined as 75 bps INFO @ Wed, 22 Jul 2020 15:07:41: #1 tag size = 75 INFO @ Wed, 22 Jul 2020 15:07:41: #1 total tags in treatment: 2008920 INFO @ Wed, 22 Jul 2020 15:07:41: #1 user defined the maximum tags... INFO @ Wed, 22 Jul 2020 15:07:41: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 22 Jul 2020 15:07:41: #1 tags after filtering in treatment: 1767955 INFO @ Wed, 22 Jul 2020 15:07:41: #1 Redundant rate of treatment: 0.12 INFO @ Wed, 22 Jul 2020 15:07:41: #1 finished! INFO @ Wed, 22 Jul 2020 15:07:41: #2 Build Peak Model... INFO @ Wed, 22 Jul 2020 15:07:41: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 22 Jul 2020 15:07:42: #2 number of paired peaks: 73 WARNING @ Wed, 22 Jul 2020 15:07:42: Too few paired peaks (73) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 22 Jul 2020 15:07:42: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8462210/SRX8462210.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8462210/SRX8462210.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8462210/SRX8462210.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8462210/SRX8462210.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 22 Jul 2020 15:07:44: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8462210/SRX8462210.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8462210/SRX8462210.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8462210/SRX8462210.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8462210/SRX8462210.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 22 Jul 2020 15:07:44: #1 read tag files... INFO @ Wed, 22 Jul 2020 15:07:44: #1 read treatment tags... INFO @ Wed, 22 Jul 2020 15:07:51: 1000000 INFO @ Wed, 22 Jul 2020 15:07:58: 2000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Wed, 22 Jul 2020 15:08:04: 3000000 BigWig に変換しました。 INFO @ Wed, 22 Jul 2020 15:08:11: 4000000 INFO @ Wed, 22 Jul 2020 15:08:12: #1 tag size is determined as 75 bps INFO @ Wed, 22 Jul 2020 15:08:12: #1 tag size = 75 INFO @ Wed, 22 Jul 2020 15:08:12: #1 total tags in treatment: 2008920 INFO @ Wed, 22 Jul 2020 15:08:12: #1 user defined the maximum tags... INFO @ Wed, 22 Jul 2020 15:08:12: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 22 Jul 2020 15:08:12: #1 tags after filtering in treatment: 1767955 INFO @ Wed, 22 Jul 2020 15:08:12: #1 Redundant rate of treatment: 0.12 INFO @ Wed, 22 Jul 2020 15:08:12: #1 finished! INFO @ Wed, 22 Jul 2020 15:08:12: #2 Build Peak Model... INFO @ Wed, 22 Jul 2020 15:08:12: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 22 Jul 2020 15:08:12: #2 number of paired peaks: 73 WARNING @ Wed, 22 Jul 2020 15:08:12: Too few paired peaks (73) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 22 Jul 2020 15:08:12: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8462210/SRX8462210.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8462210/SRX8462210.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8462210/SRX8462210.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8462210/SRX8462210.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling