Job ID = 7114641 SRX = SRX8462206 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... 2020-07-22T05:59:37 prefetch.2.10.7: 1) Downloading 'SRR11915665'... 2020-07-22T05:59:37 prefetch.2.10.7: Downloading via HTTPS... 2020-07-22T06:00:40 prefetch.2.10.7: HTTPS download succeed 2020-07-22T06:00:40 prefetch.2.10.7: 'SRR11915665' is valid 2020-07-22T06:00:40 prefetch.2.10.7: 1) 'SRR11915665' was downloaded successfully 2020-07-22T06:00:40 prefetch.2.10.7: 'SRR11915665' has 0 unresolved dependencies Read 3055993 spots for SRR11915665/SRR11915665.sra Written 3055993 spots for SRR11915665/SRR11915665.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:02:53 3055993 reads; of these: 3055993 (100.00%) were paired; of these: 183492 (6.00%) aligned concordantly 0 times 2486178 (81.35%) aligned concordantly exactly 1 time 386323 (12.64%) aligned concordantly >1 times ---- 183492 pairs aligned concordantly 0 times; of these: 16122 (8.79%) aligned discordantly 1 time ---- 167370 pairs aligned 0 times concordantly or discordantly; of these: 334740 mates make up the pairs; of these: 268981 (80.36%) aligned 0 times 51479 (15.38%) aligned exactly 1 time 14280 (4.27%) aligned >1 times 95.60% overall alignment rate Time searching: 00:02:53 Overall time: 00:02:53 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 499461 / 2883182 = 0.1732 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 22 Jul 2020 15:06:20: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8462206/SRX8462206.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8462206/SRX8462206.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8462206/SRX8462206.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8462206/SRX8462206.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 22 Jul 2020 15:06:20: #1 read tag files... INFO @ Wed, 22 Jul 2020 15:06:20: #1 read treatment tags... INFO @ Wed, 22 Jul 2020 15:06:28: 1000000 INFO @ Wed, 22 Jul 2020 15:06:36: 2000000 INFO @ Wed, 22 Jul 2020 15:06:45: 3000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 22 Jul 2020 15:06:50: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8462206/SRX8462206.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8462206/SRX8462206.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8462206/SRX8462206.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8462206/SRX8462206.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 22 Jul 2020 15:06:50: #1 read tag files... INFO @ Wed, 22 Jul 2020 15:06:50: #1 read treatment tags... INFO @ Wed, 22 Jul 2020 15:06:54: 4000000 INFO @ Wed, 22 Jul 2020 15:06:59: 1000000 INFO @ Wed, 22 Jul 2020 15:07:02: #1 tag size is determined as 75 bps INFO @ Wed, 22 Jul 2020 15:07:02: #1 tag size = 75 INFO @ Wed, 22 Jul 2020 15:07:02: #1 total tags in treatment: 2373821 INFO @ Wed, 22 Jul 2020 15:07:02: #1 user defined the maximum tags... INFO @ Wed, 22 Jul 2020 15:07:02: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 22 Jul 2020 15:07:02: #1 tags after filtering in treatment: 2069056 INFO @ Wed, 22 Jul 2020 15:07:02: #1 Redundant rate of treatment: 0.13 INFO @ Wed, 22 Jul 2020 15:07:02: #1 finished! INFO @ Wed, 22 Jul 2020 15:07:02: #2 Build Peak Model... INFO @ Wed, 22 Jul 2020 15:07:02: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 22 Jul 2020 15:07:02: #2 number of paired peaks: 61 WARNING @ Wed, 22 Jul 2020 15:07:02: Too few paired peaks (61) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 22 Jul 2020 15:07:02: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8462206/SRX8462206.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8462206/SRX8462206.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8462206/SRX8462206.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8462206/SRX8462206.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Wed, 22 Jul 2020 15:07:07: 2000000 INFO @ Wed, 22 Jul 2020 15:07:14: 3000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 22 Jul 2020 15:07:20: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8462206/SRX8462206.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8462206/SRX8462206.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8462206/SRX8462206.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8462206/SRX8462206.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 22 Jul 2020 15:07:20: #1 read tag files... INFO @ Wed, 22 Jul 2020 15:07:20: #1 read treatment tags... INFO @ Wed, 22 Jul 2020 15:07:22: 4000000 INFO @ Wed, 22 Jul 2020 15:07:28: 1000000 INFO @ Wed, 22 Jul 2020 15:07:28: #1 tag size is determined as 75 bps INFO @ Wed, 22 Jul 2020 15:07:28: #1 tag size = 75 INFO @ Wed, 22 Jul 2020 15:07:28: #1 total tags in treatment: 2373821 INFO @ Wed, 22 Jul 2020 15:07:28: #1 user defined the maximum tags... INFO @ Wed, 22 Jul 2020 15:07:28: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 22 Jul 2020 15:07:28: #1 tags after filtering in treatment: 2069056 INFO @ Wed, 22 Jul 2020 15:07:28: #1 Redundant rate of treatment: 0.13 INFO @ Wed, 22 Jul 2020 15:07:28: #1 finished! INFO @ Wed, 22 Jul 2020 15:07:28: #2 Build Peak Model... INFO @ Wed, 22 Jul 2020 15:07:28: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 22 Jul 2020 15:07:29: #2 number of paired peaks: 61 WARNING @ Wed, 22 Jul 2020 15:07:29: Too few paired peaks (61) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 22 Jul 2020 15:07:29: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8462206/SRX8462206.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8462206/SRX8462206.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8462206/SRX8462206.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8462206/SRX8462206.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Wed, 22 Jul 2020 15:07:35: 2000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Wed, 22 Jul 2020 15:07:42: 3000000 BigWig に変換しました。 INFO @ Wed, 22 Jul 2020 15:07:50: 4000000 INFO @ Wed, 22 Jul 2020 15:07:56: #1 tag size is determined as 75 bps INFO @ Wed, 22 Jul 2020 15:07:56: #1 tag size = 75 INFO @ Wed, 22 Jul 2020 15:07:56: #1 total tags in treatment: 2373821 INFO @ Wed, 22 Jul 2020 15:07:56: #1 user defined the maximum tags... INFO @ Wed, 22 Jul 2020 15:07:56: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 22 Jul 2020 15:07:56: #1 tags after filtering in treatment: 2069056 INFO @ Wed, 22 Jul 2020 15:07:56: #1 Redundant rate of treatment: 0.13 INFO @ Wed, 22 Jul 2020 15:07:56: #1 finished! INFO @ Wed, 22 Jul 2020 15:07:56: #2 Build Peak Model... INFO @ Wed, 22 Jul 2020 15:07:56: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 22 Jul 2020 15:07:56: #2 number of paired peaks: 61 WARNING @ Wed, 22 Jul 2020 15:07:56: Too few paired peaks (61) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 22 Jul 2020 15:07:56: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8462206/SRX8462206.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8462206/SRX8462206.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8462206/SRX8462206.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8462206/SRX8462206.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling