Job ID = 7113990 SRX = SRX8462186 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... 2020-07-22T05:47:53 prefetch.2.10.7: 1) Downloading 'SRR11915645'... 2020-07-22T05:47:53 prefetch.2.10.7: Downloading via HTTPS... 2020-07-22T05:49:01 prefetch.2.10.7: HTTPS download succeed 2020-07-22T05:49:02 prefetch.2.10.7: 'SRR11915645' is valid 2020-07-22T05:49:02 prefetch.2.10.7: 1) 'SRR11915645' was downloaded successfully 2020-07-22T05:49:02 prefetch.2.10.7: 'SRR11915645' has 0 unresolved dependencies Read 7160538 spots for SRR11915645/SRR11915645.sra Written 7160538 spots for SRR11915645/SRR11915645.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:05:12 7160538 reads; of these: 7160538 (100.00%) were paired; of these: 1375507 (19.21%) aligned concordantly 0 times 4839514 (67.59%) aligned concordantly exactly 1 time 945517 (13.20%) aligned concordantly >1 times ---- 1375507 pairs aligned concordantly 0 times; of these: 31837 (2.31%) aligned discordantly 1 time ---- 1343670 pairs aligned 0 times concordantly or discordantly; of these: 2687340 mates make up the pairs; of these: 2553578 (95.02%) aligned 0 times 98852 (3.68%) aligned exactly 1 time 34910 (1.30%) aligned >1 times 82.17% overall alignment rate Time searching: 00:05:12 Overall time: 00:05:12 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 8 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 2023628 / 5811158 = 0.3482 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 22 Jul 2020 14:58:46: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8462186/SRX8462186.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8462186/SRX8462186.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8462186/SRX8462186.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8462186/SRX8462186.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 22 Jul 2020 14:58:46: #1 read tag files... INFO @ Wed, 22 Jul 2020 14:58:46: #1 read treatment tags... INFO @ Wed, 22 Jul 2020 14:58:53: 1000000 INFO @ Wed, 22 Jul 2020 14:58:59: 2000000 INFO @ Wed, 22 Jul 2020 14:59:07: 3000000 INFO @ Wed, 22 Jul 2020 14:59:13: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 22 Jul 2020 14:59:16: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8462186/SRX8462186.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8462186/SRX8462186.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8462186/SRX8462186.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8462186/SRX8462186.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 22 Jul 2020 14:59:16: #1 read tag files... INFO @ Wed, 22 Jul 2020 14:59:16: #1 read treatment tags... INFO @ Wed, 22 Jul 2020 14:59:20: 5000000 INFO @ Wed, 22 Jul 2020 14:59:24: 1000000 INFO @ Wed, 22 Jul 2020 14:59:28: 6000000 INFO @ Wed, 22 Jul 2020 14:59:32: 2000000 INFO @ Wed, 22 Jul 2020 14:59:36: 7000000 INFO @ Wed, 22 Jul 2020 14:59:40: 3000000 INFO @ Wed, 22 Jul 2020 14:59:42: #1 tag size is determined as 76 bps INFO @ Wed, 22 Jul 2020 14:59:42: #1 tag size = 76 INFO @ Wed, 22 Jul 2020 14:59:42: #1 total tags in treatment: 3764733 INFO @ Wed, 22 Jul 2020 14:59:42: #1 user defined the maximum tags... INFO @ Wed, 22 Jul 2020 14:59:42: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 22 Jul 2020 14:59:43: #1 tags after filtering in treatment: 2986695 INFO @ Wed, 22 Jul 2020 14:59:43: #1 Redundant rate of treatment: 0.21 INFO @ Wed, 22 Jul 2020 14:59:43: #1 finished! INFO @ Wed, 22 Jul 2020 14:59:43: #2 Build Peak Model... INFO @ Wed, 22 Jul 2020 14:59:43: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 22 Jul 2020 14:59:43: #2 number of paired peaks: 33 WARNING @ Wed, 22 Jul 2020 14:59:43: Too few paired peaks (33) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 22 Jul 2020 14:59:43: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8462186/SRX8462186.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8462186/SRX8462186.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8462186/SRX8462186.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8462186/SRX8462186.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 22 Jul 2020 14:59:46: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8462186/SRX8462186.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8462186/SRX8462186.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8462186/SRX8462186.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8462186/SRX8462186.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 22 Jul 2020 14:59:46: #1 read tag files... INFO @ Wed, 22 Jul 2020 14:59:46: #1 read treatment tags... INFO @ Wed, 22 Jul 2020 14:59:48: 4000000 INFO @ Wed, 22 Jul 2020 14:59:57: 5000000 INFO @ Wed, 22 Jul 2020 14:59:57: 1000000 INFO @ Wed, 22 Jul 2020 15:00:05: 6000000 INFO @ Wed, 22 Jul 2020 15:00:07: 2000000 INFO @ Wed, 22 Jul 2020 15:00:14: 7000000 INFO @ Wed, 22 Jul 2020 15:00:16: 3000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Wed, 22 Jul 2020 15:00:21: #1 tag size is determined as 76 bps INFO @ Wed, 22 Jul 2020 15:00:21: #1 tag size = 76 INFO @ Wed, 22 Jul 2020 15:00:21: #1 total tags in treatment: 3764733 INFO @ Wed, 22 Jul 2020 15:00:21: #1 user defined the maximum tags... INFO @ Wed, 22 Jul 2020 15:00:21: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 22 Jul 2020 15:00:21: #1 tags after filtering in treatment: 2986695 INFO @ Wed, 22 Jul 2020 15:00:21: #1 Redundant rate of treatment: 0.21 INFO @ Wed, 22 Jul 2020 15:00:21: #1 finished! INFO @ Wed, 22 Jul 2020 15:00:21: #2 Build Peak Model... INFO @ Wed, 22 Jul 2020 15:00:21: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 22 Jul 2020 15:00:21: #2 number of paired peaks: 33 WARNING @ Wed, 22 Jul 2020 15:00:21: Too few paired peaks (33) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 22 Jul 2020 15:00:21: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8462186/SRX8462186.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8462186/SRX8462186.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8462186/SRX8462186.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8462186/SRX8462186.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Wed, 22 Jul 2020 15:00:25: 4000000 BigWig に変換しました。 INFO @ Wed, 22 Jul 2020 15:00:33: 5000000 INFO @ Wed, 22 Jul 2020 15:00:41: 6000000 INFO @ Wed, 22 Jul 2020 15:00:49: 7000000 INFO @ Wed, 22 Jul 2020 15:00:55: #1 tag size is determined as 76 bps INFO @ Wed, 22 Jul 2020 15:00:55: #1 tag size = 76 INFO @ Wed, 22 Jul 2020 15:00:55: #1 total tags in treatment: 3764733 INFO @ Wed, 22 Jul 2020 15:00:55: #1 user defined the maximum tags... INFO @ Wed, 22 Jul 2020 15:00:55: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 22 Jul 2020 15:00:55: #1 tags after filtering in treatment: 2986695 INFO @ Wed, 22 Jul 2020 15:00:55: #1 Redundant rate of treatment: 0.21 INFO @ Wed, 22 Jul 2020 15:00:55: #1 finished! INFO @ Wed, 22 Jul 2020 15:00:55: #2 Build Peak Model... INFO @ Wed, 22 Jul 2020 15:00:55: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 22 Jul 2020 15:00:55: #2 number of paired peaks: 33 WARNING @ Wed, 22 Jul 2020 15:00:55: Too few paired peaks (33) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 22 Jul 2020 15:00:55: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8462186/SRX8462186.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8462186/SRX8462186.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8462186/SRX8462186.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8462186/SRX8462186.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling