Job ID = 14520315 SRX = SRX8421798 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 18084182 spots for SRR11872091/SRR11872091.sra Written 18084182 spots for SRR11872091/SRR11872091.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:03:56 18084182 reads; of these: 18084182 (100.00%) were unpaired; of these: 728032 (4.03%) aligned 0 times 13942936 (77.10%) aligned exactly 1 time 3413214 (18.87%) aligned >1 times 95.97% overall alignment rate Time searching: 00:03:56 Overall time: 00:03:56 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 11568137 / 17356150 = 0.6665 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 19:04:53: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8421798/SRX8421798.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8421798/SRX8421798.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8421798/SRX8421798.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8421798/SRX8421798.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 19:04:53: #1 read tag files... INFO @ Sat, 15 Jan 2022 19:04:53: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 19:05:00: 1000000 INFO @ Sat, 15 Jan 2022 19:05:07: 2000000 INFO @ Sat, 15 Jan 2022 19:05:15: 3000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 19:05:22: 4000000 INFO @ Sat, 15 Jan 2022 19:05:23: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8421798/SRX8421798.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8421798/SRX8421798.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8421798/SRX8421798.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8421798/SRX8421798.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 19:05:23: #1 read tag files... INFO @ Sat, 15 Jan 2022 19:05:23: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 19:05:29: 5000000 INFO @ Sat, 15 Jan 2022 19:05:30: 1000000 INFO @ Sat, 15 Jan 2022 19:05:34: #1 tag size is determined as 101 bps INFO @ Sat, 15 Jan 2022 19:05:34: #1 tag size = 101 INFO @ Sat, 15 Jan 2022 19:05:34: #1 total tags in treatment: 5788013 INFO @ Sat, 15 Jan 2022 19:05:34: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 19:05:34: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 19:05:35: #1 tags after filtering in treatment: 5788013 INFO @ Sat, 15 Jan 2022 19:05:35: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 19:05:35: #1 finished! INFO @ Sat, 15 Jan 2022 19:05:35: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 19:05:35: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 19:05:35: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 19:05:35: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 19:05:35: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8421798/SRX8421798.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8421798/SRX8421798.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8421798/SRX8421798.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8421798/SRX8421798.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 19:05:36: 2000000 INFO @ Sat, 15 Jan 2022 19:05:43: 3000000 INFO @ Sat, 15 Jan 2022 19:05:49: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 19:05:53: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8421798/SRX8421798.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8421798/SRX8421798.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8421798/SRX8421798.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8421798/SRX8421798.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 19:05:53: #1 read tag files... INFO @ Sat, 15 Jan 2022 19:05:53: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 19:05:55: 5000000 INFO @ Sat, 15 Jan 2022 19:06:00: 1000000 INFO @ Sat, 15 Jan 2022 19:06:01: #1 tag size is determined as 101 bps INFO @ Sat, 15 Jan 2022 19:06:01: #1 tag size = 101 INFO @ Sat, 15 Jan 2022 19:06:01: #1 total tags in treatment: 5788013 INFO @ Sat, 15 Jan 2022 19:06:01: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 19:06:01: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 19:06:01: #1 tags after filtering in treatment: 5788013 INFO @ Sat, 15 Jan 2022 19:06:01: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 19:06:01: #1 finished! INFO @ Sat, 15 Jan 2022 19:06:01: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 19:06:01: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 19:06:01: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 19:06:01: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 19:06:01: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8421798/SRX8421798.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8421798/SRX8421798.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8421798/SRX8421798.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8421798/SRX8421798.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 19:06:07: 2000000 INFO @ Sat, 15 Jan 2022 19:06:14: 3000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 19:06:20: 4000000 BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 19:06:26: 5000000 INFO @ Sat, 15 Jan 2022 19:06:32: #1 tag size is determined as 101 bps INFO @ Sat, 15 Jan 2022 19:06:32: #1 tag size = 101 INFO @ Sat, 15 Jan 2022 19:06:32: #1 total tags in treatment: 5788013 INFO @ Sat, 15 Jan 2022 19:06:32: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 19:06:32: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 19:06:32: #1 tags after filtering in treatment: 5788013 INFO @ Sat, 15 Jan 2022 19:06:32: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 19:06:32: #1 finished! INFO @ Sat, 15 Jan 2022 19:06:32: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 19:06:32: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 19:06:32: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 19:06:32: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 19:06:32: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8421798/SRX8421798.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8421798/SRX8421798.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8421798/SRX8421798.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8421798/SRX8421798.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling