Job ID = 14520310 SRX = SRX8421794 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 14811570 spots for SRR11872087/SRR11872087.sra Written 14811570 spots for SRR11872087/SRR11872087.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:04:22 14811570 reads; of these: 14811570 (100.00%) were unpaired; of these: 449305 (3.03%) aligned 0 times 11859418 (80.07%) aligned exactly 1 time 2502847 (16.90%) aligned >1 times 96.97% overall alignment rate Time searching: 00:04:22 Overall time: 00:04:22 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 6729216 / 14362265 = 0.4685 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 19:04:26: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8421794/SRX8421794.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8421794/SRX8421794.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8421794/SRX8421794.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8421794/SRX8421794.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 19:04:26: #1 read tag files... INFO @ Sat, 15 Jan 2022 19:04:26: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 19:04:33: 1000000 INFO @ Sat, 15 Jan 2022 19:04:39: 2000000 INFO @ Sat, 15 Jan 2022 19:04:46: 3000000 INFO @ Sat, 15 Jan 2022 19:04:53: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 19:04:56: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8421794/SRX8421794.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8421794/SRX8421794.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8421794/SRX8421794.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8421794/SRX8421794.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 19:04:56: #1 read tag files... INFO @ Sat, 15 Jan 2022 19:04:56: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 19:05:00: 5000000 INFO @ Sat, 15 Jan 2022 19:05:04: 1000000 INFO @ Sat, 15 Jan 2022 19:05:08: 6000000 INFO @ Sat, 15 Jan 2022 19:05:13: 2000000 INFO @ Sat, 15 Jan 2022 19:05:15: 7000000 INFO @ Sat, 15 Jan 2022 19:05:20: #1 tag size is determined as 101 bps INFO @ Sat, 15 Jan 2022 19:05:20: #1 tag size = 101 INFO @ Sat, 15 Jan 2022 19:05:20: #1 total tags in treatment: 7633049 INFO @ Sat, 15 Jan 2022 19:05:20: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 19:05:20: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 19:05:21: #1 tags after filtering in treatment: 7633049 INFO @ Sat, 15 Jan 2022 19:05:21: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 19:05:21: #1 finished! INFO @ Sat, 15 Jan 2022 19:05:21: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 19:05:21: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 19:05:21: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 19:05:21: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 19:05:21: Process for pairing-model is terminated! INFO @ Sat, 15 Jan 2022 19:05:22: 3000000 cut: /home/okishinya/chipatlas/results/sacCer3/SRX8421794/SRX8421794.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8421794/SRX8421794.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8421794/SRX8421794.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8421794/SRX8421794.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 19:05:26: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8421794/SRX8421794.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8421794/SRX8421794.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8421794/SRX8421794.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8421794/SRX8421794.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 19:05:26: #1 read tag files... INFO @ Sat, 15 Jan 2022 19:05:26: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 19:05:31: 4000000 INFO @ Sat, 15 Jan 2022 19:05:34: 1000000 INFO @ Sat, 15 Jan 2022 19:05:40: 5000000 INFO @ Sat, 15 Jan 2022 19:05:43: 2000000 INFO @ Sat, 15 Jan 2022 19:05:49: 6000000 INFO @ Sat, 15 Jan 2022 19:05:51: 3000000 INFO @ Sat, 15 Jan 2022 19:05:58: 7000000 INFO @ Sat, 15 Jan 2022 19:05:59: 4000000 INFO @ Sat, 15 Jan 2022 19:06:04: #1 tag size is determined as 101 bps INFO @ Sat, 15 Jan 2022 19:06:04: #1 tag size = 101 INFO @ Sat, 15 Jan 2022 19:06:04: #1 total tags in treatment: 7633049 INFO @ Sat, 15 Jan 2022 19:06:04: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 19:06:04: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 19:06:04: #1 tags after filtering in treatment: 7633049 INFO @ Sat, 15 Jan 2022 19:06:04: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 19:06:04: #1 finished! INFO @ Sat, 15 Jan 2022 19:06:04: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 19:06:04: #2 looking for paired plus/minus strand peaks... BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 19:06:04: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 19:06:04: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 19:06:04: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8421794/SRX8421794.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8421794/SRX8421794.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8421794/SRX8421794.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8421794/SRX8421794.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 19:06:08: 5000000 BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 19:06:16: 6000000 INFO @ Sat, 15 Jan 2022 19:06:24: 7000000 INFO @ Sat, 15 Jan 2022 19:06:29: #1 tag size is determined as 101 bps INFO @ Sat, 15 Jan 2022 19:06:29: #1 tag size = 101 INFO @ Sat, 15 Jan 2022 19:06:29: #1 total tags in treatment: 7633049 INFO @ Sat, 15 Jan 2022 19:06:29: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 19:06:29: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 19:06:29: #1 tags after filtering in treatment: 7633049 INFO @ Sat, 15 Jan 2022 19:06:29: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 19:06:29: #1 finished! INFO @ Sat, 15 Jan 2022 19:06:29: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 19:06:29: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 19:06:30: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 19:06:30: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 19:06:30: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8421794/SRX8421794.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8421794/SRX8421794.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8421794/SRX8421794.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8421794/SRX8421794.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling