Job ID = 14520307 SRX = SRX8421791 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 19886694 spots for SRR11872084/SRR11872084.sra Written 19886694 spots for SRR11872084/SRR11872084.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:05:26 19886694 reads; of these: 19886694 (100.00%) were unpaired; of these: 536366 (2.70%) aligned 0 times 16499313 (82.97%) aligned exactly 1 time 2851015 (14.34%) aligned >1 times 97.30% overall alignment rate Time searching: 00:05:26 Overall time: 00:05:26 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 10272933 / 19350328 = 0.5309 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 19:05:17: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8421791/SRX8421791.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8421791/SRX8421791.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8421791/SRX8421791.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8421791/SRX8421791.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 19:05:17: #1 read tag files... INFO @ Sat, 15 Jan 2022 19:05:17: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 19:05:23: 1000000 INFO @ Sat, 15 Jan 2022 19:05:29: 2000000 INFO @ Sat, 15 Jan 2022 19:05:36: 3000000 INFO @ Sat, 15 Jan 2022 19:05:42: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 19:05:47: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8421791/SRX8421791.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8421791/SRX8421791.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8421791/SRX8421791.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8421791/SRX8421791.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 19:05:47: #1 read tag files... INFO @ Sat, 15 Jan 2022 19:05:47: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 19:05:49: 5000000 INFO @ Sat, 15 Jan 2022 19:05:52: 1000000 INFO @ Sat, 15 Jan 2022 19:05:55: 6000000 INFO @ Sat, 15 Jan 2022 19:05:58: 2000000 INFO @ Sat, 15 Jan 2022 19:06:02: 7000000 INFO @ Sat, 15 Jan 2022 19:06:03: 3000000 INFO @ Sat, 15 Jan 2022 19:06:09: 8000000 INFO @ Sat, 15 Jan 2022 19:06:09: 4000000 INFO @ Sat, 15 Jan 2022 19:06:14: 5000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 19:06:15: 9000000 INFO @ Sat, 15 Jan 2022 19:06:16: #1 tag size is determined as 101 bps INFO @ Sat, 15 Jan 2022 19:06:16: #1 tag size = 101 INFO @ Sat, 15 Jan 2022 19:06:16: #1 total tags in treatment: 9077395 INFO @ Sat, 15 Jan 2022 19:06:16: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 19:06:16: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 19:06:16: #1 tags after filtering in treatment: 9077395 INFO @ Sat, 15 Jan 2022 19:06:16: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 19:06:16: #1 finished! INFO @ Sat, 15 Jan 2022 19:06:16: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 19:06:16: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 19:06:16: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 19:06:16: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 19:06:16: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8421791/SRX8421791.05_peaks.narrowPeak: No such file or directory INFO @ Sat, 15 Jan 2022 19:06:17: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8421791/SRX8421791.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8421791/SRX8421791.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8421791/SRX8421791.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8421791/SRX8421791.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 19:06:17: #1 read tag files... INFO @ Sat, 15 Jan 2022 19:06:17: #1 read treatment tags... pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8421791/SRX8421791.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8421791/SRX8421791.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8421791/SRX8421791.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 19:06:20: 6000000 INFO @ Sat, 15 Jan 2022 19:06:22: 1000000 INFO @ Sat, 15 Jan 2022 19:06:26: 7000000 INFO @ Sat, 15 Jan 2022 19:06:28: 2000000 INFO @ Sat, 15 Jan 2022 19:06:31: 8000000 INFO @ Sat, 15 Jan 2022 19:06:33: 3000000 INFO @ Sat, 15 Jan 2022 19:06:37: 9000000 INFO @ Sat, 15 Jan 2022 19:06:37: #1 tag size is determined as 101 bps INFO @ Sat, 15 Jan 2022 19:06:37: #1 tag size = 101 INFO @ Sat, 15 Jan 2022 19:06:37: #1 total tags in treatment: 9077395 INFO @ Sat, 15 Jan 2022 19:06:37: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 19:06:37: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 19:06:37: #1 tags after filtering in treatment: 9077395 INFO @ Sat, 15 Jan 2022 19:06:37: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 19:06:37: #1 finished! INFO @ Sat, 15 Jan 2022 19:06:37: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 19:06:37: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 19:06:38: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 19:06:38: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 19:06:38: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8421791/SRX8421791.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8421791/SRX8421791.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8421791/SRX8421791.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8421791/SRX8421791.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 19:06:39: 4000000 INFO @ Sat, 15 Jan 2022 19:06:44: 5000000 INFO @ Sat, 15 Jan 2022 19:06:49: 6000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 19:06:55: 7000000 BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 19:07:00: 8000000 INFO @ Sat, 15 Jan 2022 19:07:05: 9000000 INFO @ Sat, 15 Jan 2022 19:07:06: #1 tag size is determined as 101 bps INFO @ Sat, 15 Jan 2022 19:07:06: #1 tag size = 101 INFO @ Sat, 15 Jan 2022 19:07:06: #1 total tags in treatment: 9077395 INFO @ Sat, 15 Jan 2022 19:07:06: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 19:07:06: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 19:07:06: #1 tags after filtering in treatment: 9077395 INFO @ Sat, 15 Jan 2022 19:07:06: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 19:07:06: #1 finished! INFO @ Sat, 15 Jan 2022 19:07:06: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 19:07:06: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 19:07:06: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 19:07:06: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 19:07:06: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8421791/SRX8421791.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8421791/SRX8421791.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8421791/SRX8421791.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8421791/SRX8421791.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling