Job ID = 14520295 SRX = SRX8421788 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 20735375 spots for SRR11872081/SRR11872081.sra Written 20735375 spots for SRR11872081/SRR11872081.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:10:07 20735375 reads; of these: 20735375 (100.00%) were unpaired; of these: 3367222 (16.24%) aligned 0 times 14891267 (71.82%) aligned exactly 1 time 2476886 (11.95%) aligned >1 times 83.76% overall alignment rate Time searching: 00:10:07 Overall time: 00:10:07 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 16 files... [bam_rmdupse_core] 8569754 / 17368153 = 0.4934 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 19:11:59: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8421788/SRX8421788.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8421788/SRX8421788.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8421788/SRX8421788.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8421788/SRX8421788.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 19:11:59: #1 read tag files... INFO @ Sat, 15 Jan 2022 19:11:59: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 19:12:11: 1000000 INFO @ Sat, 15 Jan 2022 19:12:22: 2000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 19:12:29: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8421788/SRX8421788.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8421788/SRX8421788.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8421788/SRX8421788.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8421788/SRX8421788.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 19:12:29: #1 read tag files... INFO @ Sat, 15 Jan 2022 19:12:29: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 19:12:32: 3000000 INFO @ Sat, 15 Jan 2022 19:12:41: 1000000 INFO @ Sat, 15 Jan 2022 19:12:44: 4000000 INFO @ Sat, 15 Jan 2022 19:12:51: 2000000 INFO @ Sat, 15 Jan 2022 19:12:55: 5000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 19:12:59: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8421788/SRX8421788.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8421788/SRX8421788.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8421788/SRX8421788.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8421788/SRX8421788.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 19:12:59: #1 read tag files... INFO @ Sat, 15 Jan 2022 19:12:59: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 19:13:02: 3000000 INFO @ Sat, 15 Jan 2022 19:13:08: 6000000 INFO @ Sat, 15 Jan 2022 19:13:12: 1000000 INFO @ Sat, 15 Jan 2022 19:13:12: 4000000 INFO @ Sat, 15 Jan 2022 19:13:20: 7000000 INFO @ Sat, 15 Jan 2022 19:13:23: 5000000 INFO @ Sat, 15 Jan 2022 19:13:24: 2000000 INFO @ Sat, 15 Jan 2022 19:13:32: 8000000 INFO @ Sat, 15 Jan 2022 19:13:34: 6000000 INFO @ Sat, 15 Jan 2022 19:13:37: 3000000 INFO @ Sat, 15 Jan 2022 19:13:42: #1 tag size is determined as 151 bps INFO @ Sat, 15 Jan 2022 19:13:42: #1 tag size = 151 INFO @ Sat, 15 Jan 2022 19:13:42: #1 total tags in treatment: 8798399 INFO @ Sat, 15 Jan 2022 19:13:42: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 19:13:42: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 19:13:42: #1 tags after filtering in treatment: 8798399 INFO @ Sat, 15 Jan 2022 19:13:42: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 19:13:42: #1 finished! INFO @ Sat, 15 Jan 2022 19:13:42: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 19:13:42: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 19:13:43: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 19:13:43: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 19:13:43: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8421788/SRX8421788.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8421788/SRX8421788.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8421788/SRX8421788.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8421788/SRX8421788.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 19:13:45: 7000000 INFO @ Sat, 15 Jan 2022 19:13:49: 4000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 19:13:55: 8000000 INFO @ Sat, 15 Jan 2022 19:14:00: 5000000 BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 19:14:03: #1 tag size is determined as 151 bps INFO @ Sat, 15 Jan 2022 19:14:03: #1 tag size = 151 INFO @ Sat, 15 Jan 2022 19:14:03: #1 total tags in treatment: 8798399 INFO @ Sat, 15 Jan 2022 19:14:03: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 19:14:03: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 19:14:03: #1 tags after filtering in treatment: 8798399 INFO @ Sat, 15 Jan 2022 19:14:03: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 19:14:03: #1 finished! INFO @ Sat, 15 Jan 2022 19:14:03: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 19:14:03: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 19:14:04: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 19:14:04: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 19:14:04: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8421788/SRX8421788.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8421788/SRX8421788.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8421788/SRX8421788.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8421788/SRX8421788.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 19:14:12: 6000000 INFO @ Sat, 15 Jan 2022 19:14:23: 7000000 INFO @ Sat, 15 Jan 2022 19:14:34: 8000000 INFO @ Sat, 15 Jan 2022 19:14:42: #1 tag size is determined as 151 bps INFO @ Sat, 15 Jan 2022 19:14:42: #1 tag size = 151 INFO @ Sat, 15 Jan 2022 19:14:42: #1 total tags in treatment: 8798399 INFO @ Sat, 15 Jan 2022 19:14:42: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 19:14:42: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 19:14:43: #1 tags after filtering in treatment: 8798399 INFO @ Sat, 15 Jan 2022 19:14:43: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 19:14:43: #1 finished! INFO @ Sat, 15 Jan 2022 19:14:43: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 19:14:43: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 19:14:43: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 19:14:43: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 19:14:43: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8421788/SRX8421788.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8421788/SRX8421788.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8421788/SRX8421788.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8421788/SRX8421788.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling