Job ID = 14520293 SRX = SRX8421786 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 9646133 spots for SRR11872079/SRR11872079.sra Written 9646133 spots for SRR11872079/SRR11872079.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:03:59 9646133 reads; of these: 9646133 (100.00%) were unpaired; of these: 701950 (7.28%) aligned 0 times 7957194 (82.49%) aligned exactly 1 time 986989 (10.23%) aligned >1 times 92.72% overall alignment rate Time searching: 00:03:59 Overall time: 00:03:59 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 3192636 / 8944183 = 0.3570 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 19:00:07: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8421786/SRX8421786.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8421786/SRX8421786.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8421786/SRX8421786.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8421786/SRX8421786.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 19:00:07: #1 read tag files... INFO @ Sat, 15 Jan 2022 19:00:07: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 19:00:15: 1000000 INFO @ Sat, 15 Jan 2022 19:00:23: 2000000 INFO @ Sat, 15 Jan 2022 19:00:32: 3000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 19:00:37: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8421786/SRX8421786.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8421786/SRX8421786.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8421786/SRX8421786.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8421786/SRX8421786.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 19:00:37: #1 read tag files... INFO @ Sat, 15 Jan 2022 19:00:37: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 19:00:40: 4000000 INFO @ Sat, 15 Jan 2022 19:00:47: 1000000 INFO @ Sat, 15 Jan 2022 19:00:50: 5000000 INFO @ Sat, 15 Jan 2022 19:00:56: 2000000 INFO @ Sat, 15 Jan 2022 19:00:57: #1 tag size is determined as 151 bps INFO @ Sat, 15 Jan 2022 19:00:57: #1 tag size = 151 INFO @ Sat, 15 Jan 2022 19:00:57: #1 total tags in treatment: 5751547 INFO @ Sat, 15 Jan 2022 19:00:57: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 19:00:57: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 19:00:57: #1 tags after filtering in treatment: 5751547 INFO @ Sat, 15 Jan 2022 19:00:57: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 19:00:57: #1 finished! INFO @ Sat, 15 Jan 2022 19:00:57: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 19:00:57: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 19:00:57: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 19:00:57: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 19:00:57: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8421786/SRX8421786.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8421786/SRX8421786.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8421786/SRX8421786.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8421786/SRX8421786.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 19:01:05: 3000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 19:01:07: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8421786/SRX8421786.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8421786/SRX8421786.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8421786/SRX8421786.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8421786/SRX8421786.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 19:01:07: #1 read tag files... INFO @ Sat, 15 Jan 2022 19:01:07: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 19:01:15: 4000000 INFO @ Sat, 15 Jan 2022 19:01:18: 1000000 INFO @ Sat, 15 Jan 2022 19:01:27: 5000000 INFO @ Sat, 15 Jan 2022 19:01:29: 2000000 INFO @ Sat, 15 Jan 2022 19:01:35: #1 tag size is determined as 151 bps INFO @ Sat, 15 Jan 2022 19:01:35: #1 tag size = 151 INFO @ Sat, 15 Jan 2022 19:01:35: #1 total tags in treatment: 5751547 INFO @ Sat, 15 Jan 2022 19:01:35: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 19:01:35: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 19:01:35: #1 tags after filtering in treatment: 5751547 INFO @ Sat, 15 Jan 2022 19:01:35: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 19:01:35: #1 finished! INFO @ Sat, 15 Jan 2022 19:01:35: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 19:01:35: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 19:01:35: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 19:01:35: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 19:01:35: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8421786/SRX8421786.10_peaks.narrowPeak: No such file or directory BedGraph に変換しました。 BigWig に変換中... pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8421786/SRX8421786.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8421786/SRX8421786.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8421786/SRX8421786.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 19:01:40: 3000000 BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 19:01:49: 4000000 INFO @ Sat, 15 Jan 2022 19:01:59: 5000000 INFO @ Sat, 15 Jan 2022 19:02:05: #1 tag size is determined as 151 bps INFO @ Sat, 15 Jan 2022 19:02:05: #1 tag size = 151 INFO @ Sat, 15 Jan 2022 19:02:05: #1 total tags in treatment: 5751547 INFO @ Sat, 15 Jan 2022 19:02:05: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 19:02:05: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 19:02:06: #1 tags after filtering in treatment: 5751547 INFO @ Sat, 15 Jan 2022 19:02:06: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 19:02:06: #1 finished! INFO @ Sat, 15 Jan 2022 19:02:06: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 19:02:06: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 19:02:06: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 19:02:06: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 19:02:06: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8421786/SRX8421786.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8421786/SRX8421786.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8421786/SRX8421786.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8421786/SRX8421786.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling