Job ID = 14520290 SRX = SRX8421783 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 13430650 spots for SRR11872076/SRR11872076.sra Written 13430650 spots for SRR11872076/SRR11872076.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:06:16 13430650 reads; of these: 13430650 (100.00%) were unpaired; of these: 3367820 (25.08%) aligned 0 times 8386757 (62.44%) aligned exactly 1 time 1676073 (12.48%) aligned >1 times 74.92% overall alignment rate Time searching: 00:06:16 Overall time: 00:06:16 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 4479341 / 10062830 = 0.4451 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 19:03:36: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8421783/SRX8421783.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8421783/SRX8421783.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8421783/SRX8421783.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8421783/SRX8421783.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 19:03:36: #1 read tag files... INFO @ Sat, 15 Jan 2022 19:03:36: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 19:03:48: 1000000 INFO @ Sat, 15 Jan 2022 19:03:59: 2000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 19:04:06: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8421783/SRX8421783.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8421783/SRX8421783.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8421783/SRX8421783.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8421783/SRX8421783.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 19:04:06: #1 read tag files... INFO @ Sat, 15 Jan 2022 19:04:06: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 19:04:11: 3000000 INFO @ Sat, 15 Jan 2022 19:04:22: 1000000 INFO @ Sat, 15 Jan 2022 19:04:23: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 19:04:35: 5000000 INFO @ Sat, 15 Jan 2022 19:04:36: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8421783/SRX8421783.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8421783/SRX8421783.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8421783/SRX8421783.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8421783/SRX8421783.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 19:04:36: #1 read tag files... INFO @ Sat, 15 Jan 2022 19:04:36: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 19:04:37: 2000000 INFO @ Sat, 15 Jan 2022 19:04:42: #1 tag size is determined as 151 bps INFO @ Sat, 15 Jan 2022 19:04:42: #1 tag size = 151 INFO @ Sat, 15 Jan 2022 19:04:42: #1 total tags in treatment: 5583489 INFO @ Sat, 15 Jan 2022 19:04:42: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 19:04:42: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 19:04:43: #1 tags after filtering in treatment: 5583489 INFO @ Sat, 15 Jan 2022 19:04:43: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 19:04:43: #1 finished! INFO @ Sat, 15 Jan 2022 19:04:43: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 19:04:43: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 19:04:43: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 19:04:43: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 19:04:43: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8421783/SRX8421783.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8421783/SRX8421783.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8421783/SRX8421783.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8421783/SRX8421783.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 19:04:50: 1000000 INFO @ Sat, 15 Jan 2022 19:04:53: 3000000 INFO @ Sat, 15 Jan 2022 19:05:03: 2000000 INFO @ Sat, 15 Jan 2022 19:05:07: 4000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 19:05:15: 3000000 INFO @ Sat, 15 Jan 2022 19:05:22: 5000000 BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 19:05:27: 4000000 INFO @ Sat, 15 Jan 2022 19:05:31: #1 tag size is determined as 151 bps INFO @ Sat, 15 Jan 2022 19:05:31: #1 tag size = 151 INFO @ Sat, 15 Jan 2022 19:05:31: #1 total tags in treatment: 5583489 INFO @ Sat, 15 Jan 2022 19:05:31: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 19:05:31: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 19:05:31: #1 tags after filtering in treatment: 5583489 INFO @ Sat, 15 Jan 2022 19:05:31: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 19:05:31: #1 finished! INFO @ Sat, 15 Jan 2022 19:05:31: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 19:05:31: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 19:05:31: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 19:05:31: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 19:05:31: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8421783/SRX8421783.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8421783/SRX8421783.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8421783/SRX8421783.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8421783/SRX8421783.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 19:05:39: 5000000 INFO @ Sat, 15 Jan 2022 19:05:46: #1 tag size is determined as 151 bps INFO @ Sat, 15 Jan 2022 19:05:46: #1 tag size = 151 INFO @ Sat, 15 Jan 2022 19:05:46: #1 total tags in treatment: 5583489 INFO @ Sat, 15 Jan 2022 19:05:46: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 19:05:46: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 19:05:46: #1 tags after filtering in treatment: 5583489 INFO @ Sat, 15 Jan 2022 19:05:46: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 19:05:46: #1 finished! INFO @ Sat, 15 Jan 2022 19:05:46: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 19:05:46: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 19:05:47: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 19:05:47: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 19:05:47: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8421783/SRX8421783.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8421783/SRX8421783.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8421783/SRX8421783.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8421783/SRX8421783.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling