Job ID = 14520256 SRX = SRX8398440 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 1607023 spots for SRR11848024/SRR11848024.sra Written 1607023 spots for SRR11848024/SRR11848024.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:00:45 1607023 reads; of these: 1607023 (100.00%) were paired; of these: 249071 (15.50%) aligned concordantly 0 times 1200511 (74.70%) aligned concordantly exactly 1 time 157441 (9.80%) aligned concordantly >1 times ---- 249071 pairs aligned concordantly 0 times; of these: 14822 (5.95%) aligned discordantly 1 time ---- 234249 pairs aligned 0 times concordantly or discordantly; of these: 468498 mates make up the pairs; of these: 457303 (97.61%) aligned 0 times 6366 (1.36%) aligned exactly 1 time 4829 (1.03%) aligned >1 times 85.77% overall alignment rate Time searching: 00:00:45 Overall time: 00:00:45 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 49395 / 1372289 = 0.0360 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 18:48:17: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8398440/SRX8398440.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8398440/SRX8398440.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8398440/SRX8398440.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8398440/SRX8398440.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 18:48:17: #1 read tag files... INFO @ Sat, 15 Jan 2022 18:48:17: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 18:48:23: 1000000 INFO @ Sat, 15 Jan 2022 18:48:30: 2000000 INFO @ Sat, 15 Jan 2022 18:48:33: #1 tag size is determined as 38 bps INFO @ Sat, 15 Jan 2022 18:48:33: #1 tag size = 38 INFO @ Sat, 15 Jan 2022 18:48:33: #1 total tags in treatment: 1308725 INFO @ Sat, 15 Jan 2022 18:48:33: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 18:48:33: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 18:48:33: #1 tags after filtering in treatment: 1063214 INFO @ Sat, 15 Jan 2022 18:48:33: #1 Redundant rate of treatment: 0.19 INFO @ Sat, 15 Jan 2022 18:48:33: #1 finished! INFO @ Sat, 15 Jan 2022 18:48:33: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 18:48:33: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 18:48:33: #2 number of paired peaks: 58 WARNING @ Sat, 15 Jan 2022 18:48:33: Too few paired peaks (58) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 18:48:33: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8398440/SRX8398440.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8398440/SRX8398440.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8398440/SRX8398440.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8398440/SRX8398440.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 18:48:47: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8398440/SRX8398440.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8398440/SRX8398440.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8398440/SRX8398440.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8398440/SRX8398440.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 18:48:47: #1 read tag files... INFO @ Sat, 15 Jan 2022 18:48:47: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 18:48:52: 1000000 INFO @ Sat, 15 Jan 2022 18:48:57: 2000000 INFO @ Sat, 15 Jan 2022 18:49:00: #1 tag size is determined as 38 bps INFO @ Sat, 15 Jan 2022 18:49:00: #1 tag size = 38 INFO @ Sat, 15 Jan 2022 18:49:00: #1 total tags in treatment: 1308725 INFO @ Sat, 15 Jan 2022 18:49:00: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 18:49:00: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 18:49:00: #1 tags after filtering in treatment: 1063214 INFO @ Sat, 15 Jan 2022 18:49:00: #1 Redundant rate of treatment: 0.19 INFO @ Sat, 15 Jan 2022 18:49:00: #1 finished! INFO @ Sat, 15 Jan 2022 18:49:00: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 18:49:00: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 18:49:00: #2 number of paired peaks: 58 WARNING @ Sat, 15 Jan 2022 18:49:00: Too few paired peaks (58) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 18:49:00: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8398440/SRX8398440.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8398440/SRX8398440.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8398440/SRX8398440.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8398440/SRX8398440.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 18:49:17: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8398440/SRX8398440.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8398440/SRX8398440.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8398440/SRX8398440.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8398440/SRX8398440.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 18:49:17: #1 read tag files... INFO @ Sat, 15 Jan 2022 18:49:17: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 18:49:22: 1000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 18:49:27: 2000000 BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 18:49:30: #1 tag size is determined as 38 bps INFO @ Sat, 15 Jan 2022 18:49:30: #1 tag size = 38 INFO @ Sat, 15 Jan 2022 18:49:30: #1 total tags in treatment: 1308725 INFO @ Sat, 15 Jan 2022 18:49:30: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 18:49:30: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 18:49:30: #1 tags after filtering in treatment: 1063214 INFO @ Sat, 15 Jan 2022 18:49:30: #1 Redundant rate of treatment: 0.19 INFO @ Sat, 15 Jan 2022 18:49:30: #1 finished! INFO @ Sat, 15 Jan 2022 18:49:30: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 18:49:30: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 18:49:30: #2 number of paired peaks: 58 WARNING @ Sat, 15 Jan 2022 18:49:30: Too few paired peaks (58) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 18:49:30: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8398440/SRX8398440.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8398440/SRX8398440.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8398440/SRX8398440.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8398440/SRX8398440.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling