Job ID = 14520223 SRX = SRX8398413 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 2027205 spots for SRR11848051/SRR11848051.sra Written 2027205 spots for SRR11848051/SRR11848051.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:01:57 2027205 reads; of these: 2027205 (100.00%) were paired; of these: 313523 (15.47%) aligned concordantly 0 times 1500283 (74.01%) aligned concordantly exactly 1 time 213399 (10.53%) aligned concordantly >1 times ---- 313523 pairs aligned concordantly 0 times; of these: 79854 (25.47%) aligned discordantly 1 time ---- 233669 pairs aligned 0 times concordantly or discordantly; of these: 467338 mates make up the pairs; of these: 418789 (89.61%) aligned 0 times 28200 (6.03%) aligned exactly 1 time 20349 (4.35%) aligned >1 times 89.67% overall alignment rate Time searching: 00:01:57 Overall time: 00:01:57 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 96093 / 1775478 = 0.0541 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 18:45:27: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8398413/SRX8398413.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8398413/SRX8398413.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8398413/SRX8398413.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8398413/SRX8398413.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 18:45:27: #1 read tag files... INFO @ Sat, 15 Jan 2022 18:45:27: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 18:45:37: 1000000 INFO @ Sat, 15 Jan 2022 18:45:46: 2000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 18:45:56: 3000000 INFO @ Sat, 15 Jan 2022 18:45:57: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8398413/SRX8398413.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8398413/SRX8398413.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8398413/SRX8398413.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8398413/SRX8398413.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 18:45:57: #1 read tag files... INFO @ Sat, 15 Jan 2022 18:45:57: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 18:46:00: #1 tag size is determined as 48 bps INFO @ Sat, 15 Jan 2022 18:46:00: #1 tag size = 48 INFO @ Sat, 15 Jan 2022 18:46:00: #1 total tags in treatment: 1618887 INFO @ Sat, 15 Jan 2022 18:46:00: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 18:46:00: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 18:46:00: #1 tags after filtering in treatment: 1285544 INFO @ Sat, 15 Jan 2022 18:46:00: #1 Redundant rate of treatment: 0.21 INFO @ Sat, 15 Jan 2022 18:46:00: #1 finished! INFO @ Sat, 15 Jan 2022 18:46:00: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 18:46:00: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 18:46:00: #2 number of paired peaks: 32 WARNING @ Sat, 15 Jan 2022 18:46:00: Too few paired peaks (32) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 18:46:00: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8398413/SRX8398413.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8398413/SRX8398413.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8398413/SRX8398413.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8398413/SRX8398413.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 18:46:06: 1000000 INFO @ Sat, 15 Jan 2022 18:46:14: 2000000 INFO @ Sat, 15 Jan 2022 18:46:22: 3000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 18:46:26: #1 tag size is determined as 48 bps INFO @ Sat, 15 Jan 2022 18:46:26: #1 tag size = 48 INFO @ Sat, 15 Jan 2022 18:46:26: #1 total tags in treatment: 1618887 INFO @ Sat, 15 Jan 2022 18:46:26: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 18:46:26: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 18:46:26: #1 tags after filtering in treatment: 1285544 INFO @ Sat, 15 Jan 2022 18:46:26: #1 Redundant rate of treatment: 0.21 INFO @ Sat, 15 Jan 2022 18:46:26: #1 finished! INFO @ Sat, 15 Jan 2022 18:46:26: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 18:46:26: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 18:46:26: #2 number of paired peaks: 32 WARNING @ Sat, 15 Jan 2022 18:46:26: Too few paired peaks (32) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 18:46:26: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8398413/SRX8398413.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8398413/SRX8398413.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8398413/SRX8398413.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8398413/SRX8398413.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 18:46:27: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8398413/SRX8398413.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8398413/SRX8398413.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8398413/SRX8398413.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8398413/SRX8398413.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 18:46:27: #1 read tag files... INFO @ Sat, 15 Jan 2022 18:46:27: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 18:46:37: 1000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 18:46:47: 2000000 BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 18:46:56: 3000000 INFO @ Sat, 15 Jan 2022 18:47:01: #1 tag size is determined as 48 bps INFO @ Sat, 15 Jan 2022 18:47:01: #1 tag size = 48 INFO @ Sat, 15 Jan 2022 18:47:01: #1 total tags in treatment: 1618887 INFO @ Sat, 15 Jan 2022 18:47:01: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 18:47:01: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 18:47:01: #1 tags after filtering in treatment: 1285544 INFO @ Sat, 15 Jan 2022 18:47:01: #1 Redundant rate of treatment: 0.21 INFO @ Sat, 15 Jan 2022 18:47:01: #1 finished! INFO @ Sat, 15 Jan 2022 18:47:01: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 18:47:01: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 18:47:01: #2 number of paired peaks: 32 WARNING @ Sat, 15 Jan 2022 18:47:01: Too few paired peaks (32) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 18:47:01: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8398413/SRX8398413.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8398413/SRX8398413.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8398413/SRX8398413.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8398413/SRX8398413.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling