Job ID = 8184696 SRX = SRX8362528 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-08-10T05:12:47 prefetch.2.10.7: 1) Downloading 'SRR11811255'... 2020-08-10T05:12:47 prefetch.2.10.7: Downloading via HTTPS... 2020-08-10T05:13:16 prefetch.2.10.7: HTTPS download succeed 2020-08-10T05:13:16 prefetch.2.10.7: 'SRR11811255' is valid 2020-08-10T05:13:16 prefetch.2.10.7: 1) 'SRR11811255' was downloaded successfully Read 4836742 spots for SRR11811255/SRR11811255.sra Written 4836742 spots for SRR11811255/SRR11811255.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:00:29 4836742 reads; of these: 4836742 (100.00%) were unpaired; of these: 500082 (10.34%) aligned 0 times 3316725 (68.57%) aligned exactly 1 time 1019935 (21.09%) aligned >1 times 89.66% overall alignment rate Time searching: 00:00:29 Overall time: 00:00:29 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_rmdupse_core] 1161077 / 4336660 = 0.2677 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Mon, 10 Aug 2020 14:15:15: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8362528/SRX8362528.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8362528/SRX8362528.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8362528/SRX8362528.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8362528/SRX8362528.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 10 Aug 2020 14:15:15: #1 read tag files... INFO @ Mon, 10 Aug 2020 14:15:15: #1 read treatment tags... INFO @ Mon, 10 Aug 2020 14:15:20: 1000000 INFO @ Mon, 10 Aug 2020 14:15:25: 2000000 INFO @ Mon, 10 Aug 2020 14:15:30: 3000000 INFO @ Mon, 10 Aug 2020 14:15:31: #1 tag size is determined as 37 bps INFO @ Mon, 10 Aug 2020 14:15:31: #1 tag size = 37 INFO @ Mon, 10 Aug 2020 14:15:31: #1 total tags in treatment: 3175583 INFO @ Mon, 10 Aug 2020 14:15:31: #1 user defined the maximum tags... INFO @ Mon, 10 Aug 2020 14:15:31: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 10 Aug 2020 14:15:31: #1 tags after filtering in treatment: 3175583 INFO @ Mon, 10 Aug 2020 14:15:31: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 10 Aug 2020 14:15:31: #1 finished! INFO @ Mon, 10 Aug 2020 14:15:31: #2 Build Peak Model... INFO @ Mon, 10 Aug 2020 14:15:31: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 10 Aug 2020 14:15:32: #2 number of paired peaks: 31 WARNING @ Mon, 10 Aug 2020 14:15:32: Too few paired peaks (31) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 10 Aug 2020 14:15:32: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8362528/SRX8362528.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8362528/SRX8362528.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8362528/SRX8362528.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8362528/SRX8362528.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Mon, 10 Aug 2020 14:15:45: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8362528/SRX8362528.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8362528/SRX8362528.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8362528/SRX8362528.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8362528/SRX8362528.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 10 Aug 2020 14:15:45: #1 read tag files... INFO @ Mon, 10 Aug 2020 14:15:45: #1 read treatment tags... INFO @ Mon, 10 Aug 2020 14:15:51: 1000000 INFO @ Mon, 10 Aug 2020 14:15:56: 2000000 INFO @ Mon, 10 Aug 2020 14:16:02: 3000000 INFO @ Mon, 10 Aug 2020 14:16:03: #1 tag size is determined as 37 bps INFO @ Mon, 10 Aug 2020 14:16:03: #1 tag size = 37 INFO @ Mon, 10 Aug 2020 14:16:03: #1 total tags in treatment: 3175583 INFO @ Mon, 10 Aug 2020 14:16:03: #1 user defined the maximum tags... INFO @ Mon, 10 Aug 2020 14:16:03: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 10 Aug 2020 14:16:03: #1 tags after filtering in treatment: 3175583 INFO @ Mon, 10 Aug 2020 14:16:03: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 10 Aug 2020 14:16:03: #1 finished! INFO @ Mon, 10 Aug 2020 14:16:03: #2 Build Peak Model... INFO @ Mon, 10 Aug 2020 14:16:03: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 10 Aug 2020 14:16:03: #2 number of paired peaks: 31 WARNING @ Mon, 10 Aug 2020 14:16:03: Too few paired peaks (31) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 10 Aug 2020 14:16:03: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8362528/SRX8362528.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8362528/SRX8362528.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8362528/SRX8362528.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8362528/SRX8362528.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Mon, 10 Aug 2020 14:16:15: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8362528/SRX8362528.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8362528/SRX8362528.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8362528/SRX8362528.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8362528/SRX8362528.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 10 Aug 2020 14:16:15: #1 read tag files... INFO @ Mon, 10 Aug 2020 14:16:15: #1 read treatment tags... INFO @ Mon, 10 Aug 2020 14:16:22: 1000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Mon, 10 Aug 2020 14:16:28: 2000000 BigWig に変換しました。 INFO @ Mon, 10 Aug 2020 14:16:34: 3000000 INFO @ Mon, 10 Aug 2020 14:16:36: #1 tag size is determined as 37 bps INFO @ Mon, 10 Aug 2020 14:16:36: #1 tag size = 37 INFO @ Mon, 10 Aug 2020 14:16:36: #1 total tags in treatment: 3175583 INFO @ Mon, 10 Aug 2020 14:16:36: #1 user defined the maximum tags... INFO @ Mon, 10 Aug 2020 14:16:36: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 10 Aug 2020 14:16:36: #1 tags after filtering in treatment: 3175583 INFO @ Mon, 10 Aug 2020 14:16:36: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 10 Aug 2020 14:16:36: #1 finished! INFO @ Mon, 10 Aug 2020 14:16:36: #2 Build Peak Model... INFO @ Mon, 10 Aug 2020 14:16:36: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 10 Aug 2020 14:16:36: #2 number of paired peaks: 31 WARNING @ Mon, 10 Aug 2020 14:16:36: Too few paired peaks (31) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 10 Aug 2020 14:16:36: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8362528/SRX8362528.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8362528/SRX8362528.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8362528/SRX8362528.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8362528/SRX8362528.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling