Job ID = 8184694 SRX = SRX8362526 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-08-10T05:10:01 prefetch.2.10.7: 1) Downloading 'SRR11811253'... 2020-08-10T05:10:01 prefetch.2.10.7: Downloading via HTTPS... 2020-08-10T05:10:34 prefetch.2.10.7: HTTPS download succeed 2020-08-10T05:10:34 prefetch.2.10.7: 'SRR11811253' is valid 2020-08-10T05:10:34 prefetch.2.10.7: 1) 'SRR11811253' was downloaded successfully Read 6936475 spots for SRR11811253/SRR11811253.sra Written 6936475 spots for SRR11811253/SRR11811253.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:00:36 6936475 reads; of these: 6936475 (100.00%) were unpaired; of these: 688857 (9.93%) aligned 0 times 5165186 (74.46%) aligned exactly 1 time 1082432 (15.60%) aligned >1 times 90.07% overall alignment rate Time searching: 00:00:36 Overall time: 00:00:36 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_rmdupse_core] 1517284 / 6247618 = 0.2429 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Mon, 10 Aug 2020 14:13:04: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8362526/SRX8362526.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8362526/SRX8362526.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8362526/SRX8362526.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8362526/SRX8362526.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 10 Aug 2020 14:13:04: #1 read tag files... INFO @ Mon, 10 Aug 2020 14:13:04: #1 read treatment tags... INFO @ Mon, 10 Aug 2020 14:13:08: 1000000 INFO @ Mon, 10 Aug 2020 14:13:13: 2000000 INFO @ Mon, 10 Aug 2020 14:13:17: 3000000 INFO @ Mon, 10 Aug 2020 14:13:22: 4000000 INFO @ Mon, 10 Aug 2020 14:13:25: #1 tag size is determined as 37 bps INFO @ Mon, 10 Aug 2020 14:13:25: #1 tag size = 37 INFO @ Mon, 10 Aug 2020 14:13:25: #1 total tags in treatment: 4730334 INFO @ Mon, 10 Aug 2020 14:13:25: #1 user defined the maximum tags... INFO @ Mon, 10 Aug 2020 14:13:25: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 10 Aug 2020 14:13:25: #1 tags after filtering in treatment: 4730334 INFO @ Mon, 10 Aug 2020 14:13:25: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 10 Aug 2020 14:13:25: #1 finished! INFO @ Mon, 10 Aug 2020 14:13:25: #2 Build Peak Model... INFO @ Mon, 10 Aug 2020 14:13:25: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 10 Aug 2020 14:13:25: #2 number of paired peaks: 25 WARNING @ Mon, 10 Aug 2020 14:13:25: Too few paired peaks (25) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 10 Aug 2020 14:13:25: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8362526/SRX8362526.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8362526/SRX8362526.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8362526/SRX8362526.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8362526/SRX8362526.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Mon, 10 Aug 2020 14:13:34: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8362526/SRX8362526.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8362526/SRX8362526.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8362526/SRX8362526.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8362526/SRX8362526.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 10 Aug 2020 14:13:34: #1 read tag files... INFO @ Mon, 10 Aug 2020 14:13:34: #1 read treatment tags... INFO @ Mon, 10 Aug 2020 14:13:38: 1000000 INFO @ Mon, 10 Aug 2020 14:13:43: 2000000 INFO @ Mon, 10 Aug 2020 14:13:47: 3000000 INFO @ Mon, 10 Aug 2020 14:13:52: 4000000 INFO @ Mon, 10 Aug 2020 14:13:55: #1 tag size is determined as 37 bps INFO @ Mon, 10 Aug 2020 14:13:55: #1 tag size = 37 INFO @ Mon, 10 Aug 2020 14:13:55: #1 total tags in treatment: 4730334 INFO @ Mon, 10 Aug 2020 14:13:55: #1 user defined the maximum tags... INFO @ Mon, 10 Aug 2020 14:13:55: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 10 Aug 2020 14:13:55: #1 tags after filtering in treatment: 4730334 INFO @ Mon, 10 Aug 2020 14:13:55: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 10 Aug 2020 14:13:55: #1 finished! INFO @ Mon, 10 Aug 2020 14:13:55: #2 Build Peak Model... INFO @ Mon, 10 Aug 2020 14:13:55: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 10 Aug 2020 14:13:55: #2 number of paired peaks: 25 WARNING @ Mon, 10 Aug 2020 14:13:55: Too few paired peaks (25) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 10 Aug 2020 14:13:55: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8362526/SRX8362526.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8362526/SRX8362526.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8362526/SRX8362526.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8362526/SRX8362526.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Mon, 10 Aug 2020 14:14:04: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8362526/SRX8362526.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8362526/SRX8362526.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8362526/SRX8362526.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8362526/SRX8362526.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 10 Aug 2020 14:14:04: #1 read tag files... INFO @ Mon, 10 Aug 2020 14:14:04: #1 read treatment tags... INFO @ Mon, 10 Aug 2020 14:14:08: 1000000 INFO @ Mon, 10 Aug 2020 14:14:13: 2000000 INFO @ Mon, 10 Aug 2020 14:14:17: 3000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Mon, 10 Aug 2020 14:14:22: 4000000 INFO @ Mon, 10 Aug 2020 14:14:25: #1 tag size is determined as 37 bps INFO @ Mon, 10 Aug 2020 14:14:25: #1 tag size = 37 INFO @ Mon, 10 Aug 2020 14:14:25: #1 total tags in treatment: 4730334 INFO @ Mon, 10 Aug 2020 14:14:25: #1 user defined the maximum tags... INFO @ Mon, 10 Aug 2020 14:14:25: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 10 Aug 2020 14:14:25: #1 tags after filtering in treatment: 4730334 INFO @ Mon, 10 Aug 2020 14:14:25: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 10 Aug 2020 14:14:25: #1 finished! INFO @ Mon, 10 Aug 2020 14:14:25: #2 Build Peak Model... INFO @ Mon, 10 Aug 2020 14:14:25: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 10 Aug 2020 14:14:25: #2 number of paired peaks: 25 WARNING @ Mon, 10 Aug 2020 14:14:25: Too few paired peaks (25) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 10 Aug 2020 14:14:25: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8362526/SRX8362526.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8362526/SRX8362526.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8362526/SRX8362526.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8362526/SRX8362526.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。