Job ID = 7113268 SRX = SRX8357702 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... 2020-07-22T05:28:29 prefetch.2.10.7: 1) Downloading 'SRR11806290'... 2020-07-22T05:28:29 prefetch.2.10.7: Downloading via HTTPS... 2020-07-22T05:29:16 prefetch.2.10.7: HTTPS download succeed 2020-07-22T05:29:17 prefetch.2.10.7: 'SRR11806290' is valid 2020-07-22T05:29:17 prefetch.2.10.7: 1) 'SRR11806290' was downloaded successfully 2020-07-22T05:29:17 prefetch.2.10.7: 'SRR11806290' has 0 unresolved dependencies Read 11190535 spots for SRR11806290/SRR11806290.sra Written 11190535 spots for SRR11806290/SRR11806290.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:06:09 11190535 reads; of these: 11190535 (100.00%) were paired; of these: 624912 (5.58%) aligned concordantly 0 times 9634793 (86.10%) aligned concordantly exactly 1 time 930830 (8.32%) aligned concordantly >1 times ---- 624912 pairs aligned concordantly 0 times; of these: 47681 (7.63%) aligned discordantly 1 time ---- 577231 pairs aligned 0 times concordantly or discordantly; of these: 1154462 mates make up the pairs; of these: 975414 (84.49%) aligned 0 times 130739 (11.32%) aligned exactly 1 time 48309 (4.18%) aligned >1 times 95.64% overall alignment rate Time searching: 00:06:09 Overall time: 00:06:09 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 12 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 1487381 / 10594498 = 0.1404 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 22 Jul 2020 14:41:42: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8357702/SRX8357702.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8357702/SRX8357702.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8357702/SRX8357702.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8357702/SRX8357702.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 22 Jul 2020 14:41:42: #1 read tag files... INFO @ Wed, 22 Jul 2020 14:41:42: #1 read treatment tags... INFO @ Wed, 22 Jul 2020 14:41:48: 1000000 INFO @ Wed, 22 Jul 2020 14:41:54: 2000000 INFO @ Wed, 22 Jul 2020 14:42:00: 3000000 INFO @ Wed, 22 Jul 2020 14:42:05: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 22 Jul 2020 14:42:11: 5000000 INFO @ Wed, 22 Jul 2020 14:42:12: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8357702/SRX8357702.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8357702/SRX8357702.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8357702/SRX8357702.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8357702/SRX8357702.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 22 Jul 2020 14:42:12: #1 read tag files... INFO @ Wed, 22 Jul 2020 14:42:12: #1 read treatment tags... INFO @ Wed, 22 Jul 2020 14:42:17: 6000000 INFO @ Wed, 22 Jul 2020 14:42:18: 1000000 INFO @ Wed, 22 Jul 2020 14:42:24: 7000000 INFO @ Wed, 22 Jul 2020 14:42:24: 2000000 INFO @ Wed, 22 Jul 2020 14:42:30: 8000000 INFO @ Wed, 22 Jul 2020 14:42:31: 3000000 INFO @ Wed, 22 Jul 2020 14:42:36: 9000000 INFO @ Wed, 22 Jul 2020 14:42:37: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 22 Jul 2020 14:42:42: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8357702/SRX8357702.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8357702/SRX8357702.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8357702/SRX8357702.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8357702/SRX8357702.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 22 Jul 2020 14:42:42: #1 read tag files... INFO @ Wed, 22 Jul 2020 14:42:42: #1 read treatment tags... INFO @ Wed, 22 Jul 2020 14:42:42: 10000000 INFO @ Wed, 22 Jul 2020 14:42:43: 5000000 INFO @ Wed, 22 Jul 2020 14:42:48: 1000000 INFO @ Wed, 22 Jul 2020 14:42:48: 11000000 INFO @ Wed, 22 Jul 2020 14:42:49: 6000000 INFO @ Wed, 22 Jul 2020 14:42:54: 2000000 INFO @ Wed, 22 Jul 2020 14:42:54: 12000000 INFO @ Wed, 22 Jul 2020 14:42:55: 7000000 INFO @ Wed, 22 Jul 2020 14:43:00: 3000000 INFO @ Wed, 22 Jul 2020 14:43:01: 13000000 INFO @ Wed, 22 Jul 2020 14:43:01: 8000000 INFO @ Wed, 22 Jul 2020 14:43:06: 4000000 INFO @ Wed, 22 Jul 2020 14:43:07: 14000000 INFO @ Wed, 22 Jul 2020 14:43:08: 9000000 INFO @ Wed, 22 Jul 2020 14:43:12: 5000000 INFO @ Wed, 22 Jul 2020 14:43:13: 15000000 INFO @ Wed, 22 Jul 2020 14:43:14: 10000000 INFO @ Wed, 22 Jul 2020 14:43:19: 6000000 INFO @ Wed, 22 Jul 2020 14:43:19: 16000000 INFO @ Wed, 22 Jul 2020 14:43:20: 11000000 INFO @ Wed, 22 Jul 2020 14:43:25: 7000000 INFO @ Wed, 22 Jul 2020 14:43:25: 17000000 INFO @ Wed, 22 Jul 2020 14:43:26: 12000000 INFO @ Wed, 22 Jul 2020 14:43:31: 8000000 INFO @ Wed, 22 Jul 2020 14:43:31: 18000000 INFO @ Wed, 22 Jul 2020 14:43:33: 13000000 INFO @ Wed, 22 Jul 2020 14:43:34: #1 tag size is determined as 51 bps INFO @ Wed, 22 Jul 2020 14:43:34: #1 tag size = 51 INFO @ Wed, 22 Jul 2020 14:43:34: #1 total tags in treatment: 9081859 INFO @ Wed, 22 Jul 2020 14:43:34: #1 user defined the maximum tags... INFO @ Wed, 22 Jul 2020 14:43:34: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 22 Jul 2020 14:43:34: #1 tags after filtering in treatment: 4902527 INFO @ Wed, 22 Jul 2020 14:43:34: #1 Redundant rate of treatment: 0.46 INFO @ Wed, 22 Jul 2020 14:43:34: #1 finished! INFO @ Wed, 22 Jul 2020 14:43:34: #2 Build Peak Model... INFO @ Wed, 22 Jul 2020 14:43:34: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 22 Jul 2020 14:43:35: #2 number of paired peaks: 0 WARNING @ Wed, 22 Jul 2020 14:43:35: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 22 Jul 2020 14:43:35: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8357702/SRX8357702.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8357702/SRX8357702.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8357702/SRX8357702.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8357702/SRX8357702.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... INFO @ Wed, 22 Jul 2020 14:43:37: 9000000 INFO @ Wed, 22 Jul 2020 14:43:39: 14000000 INFO @ Wed, 22 Jul 2020 14:43:43: 10000000 INFO @ Wed, 22 Jul 2020 14:43:45: 15000000 BigWig に変換しました。 INFO @ Wed, 22 Jul 2020 14:43:49: 11000000 INFO @ Wed, 22 Jul 2020 14:43:51: 16000000 INFO @ Wed, 22 Jul 2020 14:43:55: 12000000 INFO @ Wed, 22 Jul 2020 14:43:58: 17000000 INFO @ Wed, 22 Jul 2020 14:44:01: 13000000 INFO @ Wed, 22 Jul 2020 14:44:04: 18000000 INFO @ Wed, 22 Jul 2020 14:44:06: #1 tag size is determined as 51 bps INFO @ Wed, 22 Jul 2020 14:44:06: #1 tag size = 51 INFO @ Wed, 22 Jul 2020 14:44:06: #1 total tags in treatment: 9081859 INFO @ Wed, 22 Jul 2020 14:44:06: #1 user defined the maximum tags... INFO @ Wed, 22 Jul 2020 14:44:06: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 22 Jul 2020 14:44:07: #1 tags after filtering in treatment: 4902527 INFO @ Wed, 22 Jul 2020 14:44:07: #1 Redundant rate of treatment: 0.46 INFO @ Wed, 22 Jul 2020 14:44:07: #1 finished! INFO @ Wed, 22 Jul 2020 14:44:07: #2 Build Peak Model... INFO @ Wed, 22 Jul 2020 14:44:07: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 22 Jul 2020 14:44:07: #2 number of paired peaks: 0 WARNING @ Wed, 22 Jul 2020 14:44:07: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 22 Jul 2020 14:44:07: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8357702/SRX8357702.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8357702/SRX8357702.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8357702/SRX8357702.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8357702/SRX8357702.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Wed, 22 Jul 2020 14:44:07: 14000000 INFO @ Wed, 22 Jul 2020 14:44:13: 15000000 INFO @ Wed, 22 Jul 2020 14:44:19: 16000000 INFO @ Wed, 22 Jul 2020 14:44:25: 17000000 INFO @ Wed, 22 Jul 2020 14:44:30: 18000000 INFO @ Wed, 22 Jul 2020 14:44:33: #1 tag size is determined as 51 bps INFO @ Wed, 22 Jul 2020 14:44:33: #1 tag size = 51 INFO @ Wed, 22 Jul 2020 14:44:33: #1 total tags in treatment: 9081859 INFO @ Wed, 22 Jul 2020 14:44:33: #1 user defined the maximum tags... INFO @ Wed, 22 Jul 2020 14:44:33: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 22 Jul 2020 14:44:33: #1 tags after filtering in treatment: 4902527 INFO @ Wed, 22 Jul 2020 14:44:33: #1 Redundant rate of treatment: 0.46 INFO @ Wed, 22 Jul 2020 14:44:33: #1 finished! INFO @ Wed, 22 Jul 2020 14:44:33: #2 Build Peak Model... INFO @ Wed, 22 Jul 2020 14:44:33: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 22 Jul 2020 14:44:33: #2 number of paired peaks: 0 WARNING @ Wed, 22 Jul 2020 14:44:33: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 22 Jul 2020 14:44:33: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8357702/SRX8357702.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8357702/SRX8357702.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8357702/SRX8357702.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8357702/SRX8357702.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling