Job ID = 7112761 SRX = SRX8357692 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... 2020-07-22T05:23:18 prefetch.2.10.7: 1) Downloading 'SRR11806280'... 2020-07-22T05:23:18 prefetch.2.10.7: Downloading via HTTPS... 2020-07-22T05:26:06 prefetch.2.10.7: HTTPS download succeed 2020-07-22T05:26:06 prefetch.2.10.7: 1) 'SRR11806280' was downloaded successfully 2020-07-22T05:26:06 prefetch.2.10.7: 'SRR11806280' has 0 unresolved dependencies Read 43155730 spots for SRR11806280/SRR11806280.sra Written 43155730 spots for SRR11806280/SRR11806280.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:23:01 43155730 reads; of these: 43155730 (100.00%) were paired; of these: 2134444 (4.95%) aligned concordantly 0 times 37174809 (86.14%) aligned concordantly exactly 1 time 3846477 (8.91%) aligned concordantly >1 times ---- 2134444 pairs aligned concordantly 0 times; of these: 350836 (16.44%) aligned discordantly 1 time ---- 1783608 pairs aligned 0 times concordantly or discordantly; of these: 3567216 mates make up the pairs; of these: 2876883 (80.65%) aligned 0 times 280561 (7.86%) aligned exactly 1 time 409772 (11.49%) aligned >1 times 96.67% overall alignment rate Time searching: 00:23:01 Overall time: 00:23:01 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 36 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 15642833 / 41270842 = 0.3790 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 22 Jul 2020 15:05:05: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8357692/SRX8357692.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8357692/SRX8357692.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8357692/SRX8357692.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8357692/SRX8357692.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 22 Jul 2020 15:05:05: #1 read tag files... INFO @ Wed, 22 Jul 2020 15:05:05: #1 read treatment tags... INFO @ Wed, 22 Jul 2020 15:05:09: 1000000 INFO @ Wed, 22 Jul 2020 15:05:14: 2000000 INFO @ Wed, 22 Jul 2020 15:05:18: 3000000 INFO @ Wed, 22 Jul 2020 15:05:23: 4000000 INFO @ Wed, 22 Jul 2020 15:05:27: 5000000 INFO @ Wed, 22 Jul 2020 15:05:32: 6000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 22 Jul 2020 15:05:35: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8357692/SRX8357692.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8357692/SRX8357692.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8357692/SRX8357692.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8357692/SRX8357692.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 22 Jul 2020 15:05:35: #1 read tag files... INFO @ Wed, 22 Jul 2020 15:05:35: #1 read treatment tags... INFO @ Wed, 22 Jul 2020 15:05:36: 7000000 INFO @ Wed, 22 Jul 2020 15:05:39: 1000000 INFO @ Wed, 22 Jul 2020 15:05:41: 8000000 INFO @ Wed, 22 Jul 2020 15:05:44: 2000000 INFO @ Wed, 22 Jul 2020 15:05:46: 9000000 INFO @ Wed, 22 Jul 2020 15:05:49: 3000000 INFO @ Wed, 22 Jul 2020 15:05:50: 10000000 INFO @ Wed, 22 Jul 2020 15:05:53: 4000000 INFO @ Wed, 22 Jul 2020 15:05:55: 11000000 INFO @ Wed, 22 Jul 2020 15:05:58: 5000000 INFO @ Wed, 22 Jul 2020 15:06:00: 12000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 22 Jul 2020 15:06:03: 6000000 INFO @ Wed, 22 Jul 2020 15:06:04: 13000000 INFO @ Wed, 22 Jul 2020 15:06:05: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8357692/SRX8357692.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8357692/SRX8357692.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8357692/SRX8357692.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8357692/SRX8357692.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 22 Jul 2020 15:06:05: #1 read tag files... INFO @ Wed, 22 Jul 2020 15:06:05: #1 read treatment tags... INFO @ Wed, 22 Jul 2020 15:06:08: 7000000 INFO @ Wed, 22 Jul 2020 15:06:09: 14000000 INFO @ Wed, 22 Jul 2020 15:06:09: 1000000 INFO @ Wed, 22 Jul 2020 15:06:12: 8000000 INFO @ Wed, 22 Jul 2020 15:06:14: 15000000 INFO @ Wed, 22 Jul 2020 15:06:14: 2000000 INFO @ Wed, 22 Jul 2020 15:06:17: 9000000 INFO @ Wed, 22 Jul 2020 15:06:18: 16000000 INFO @ Wed, 22 Jul 2020 15:06:19: 3000000 INFO @ Wed, 22 Jul 2020 15:06:22: 10000000 INFO @ Wed, 22 Jul 2020 15:06:23: 17000000 INFO @ Wed, 22 Jul 2020 15:06:24: 4000000 INFO @ Wed, 22 Jul 2020 15:06:26: 11000000 INFO @ Wed, 22 Jul 2020 15:06:28: 18000000 INFO @ Wed, 22 Jul 2020 15:06:28: 5000000 INFO @ Wed, 22 Jul 2020 15:06:31: 12000000 INFO @ Wed, 22 Jul 2020 15:06:33: 19000000 INFO @ Wed, 22 Jul 2020 15:06:33: 6000000 INFO @ Wed, 22 Jul 2020 15:06:36: 13000000 INFO @ Wed, 22 Jul 2020 15:06:37: 20000000 INFO @ Wed, 22 Jul 2020 15:06:38: 7000000 INFO @ Wed, 22 Jul 2020 15:06:41: 14000000 INFO @ Wed, 22 Jul 2020 15:06:42: 21000000 INFO @ Wed, 22 Jul 2020 15:06:42: 8000000 INFO @ Wed, 22 Jul 2020 15:06:45: 15000000 INFO @ Wed, 22 Jul 2020 15:06:47: 22000000 INFO @ Wed, 22 Jul 2020 15:06:47: 9000000 INFO @ Wed, 22 Jul 2020 15:06:50: 16000000 INFO @ Wed, 22 Jul 2020 15:06:51: 23000000 INFO @ Wed, 22 Jul 2020 15:06:52: 10000000 INFO @ Wed, 22 Jul 2020 15:06:55: 17000000 INFO @ Wed, 22 Jul 2020 15:06:56: 24000000 INFO @ Wed, 22 Jul 2020 15:06:56: 11000000 INFO @ Wed, 22 Jul 2020 15:06:59: 18000000 INFO @ Wed, 22 Jul 2020 15:07:01: 25000000 INFO @ Wed, 22 Jul 2020 15:07:01: 12000000 INFO @ Wed, 22 Jul 2020 15:07:04: 19000000 INFO @ Wed, 22 Jul 2020 15:07:05: 26000000 INFO @ Wed, 22 Jul 2020 15:07:06: 13000000 INFO @ Wed, 22 Jul 2020 15:07:09: 20000000 INFO @ Wed, 22 Jul 2020 15:07:10: 27000000 INFO @ Wed, 22 Jul 2020 15:07:10: 14000000 INFO @ Wed, 22 Jul 2020 15:07:14: 21000000 INFO @ Wed, 22 Jul 2020 15:07:15: 28000000 INFO @ Wed, 22 Jul 2020 15:07:15: 15000000 INFO @ Wed, 22 Jul 2020 15:07:18: 22000000 INFO @ Wed, 22 Jul 2020 15:07:20: 29000000 INFO @ Wed, 22 Jul 2020 15:07:20: 16000000 INFO @ Wed, 22 Jul 2020 15:07:23: 23000000 INFO @ Wed, 22 Jul 2020 15:07:24: 30000000 INFO @ Wed, 22 Jul 2020 15:07:25: 17000000 INFO @ Wed, 22 Jul 2020 15:07:28: 24000000 INFO @ Wed, 22 Jul 2020 15:07:29: 31000000 INFO @ Wed, 22 Jul 2020 15:07:29: 18000000 INFO @ Wed, 22 Jul 2020 15:07:33: 25000000 INFO @ Wed, 22 Jul 2020 15:07:34: 32000000 INFO @ Wed, 22 Jul 2020 15:07:34: 19000000 INFO @ Wed, 22 Jul 2020 15:07:37: 26000000 INFO @ Wed, 22 Jul 2020 15:07:38: 33000000 INFO @ Wed, 22 Jul 2020 15:07:39: 20000000 INFO @ Wed, 22 Jul 2020 15:07:42: 27000000 INFO @ Wed, 22 Jul 2020 15:07:43: 34000000 INFO @ Wed, 22 Jul 2020 15:07:43: 21000000 INFO @ Wed, 22 Jul 2020 15:07:47: 28000000 INFO @ Wed, 22 Jul 2020 15:07:48: 35000000 INFO @ Wed, 22 Jul 2020 15:07:48: 22000000 INFO @ Wed, 22 Jul 2020 15:07:52: 29000000 INFO @ Wed, 22 Jul 2020 15:07:52: 36000000 INFO @ Wed, 22 Jul 2020 15:07:53: 23000000 INFO @ Wed, 22 Jul 2020 15:07:56: 30000000 INFO @ Wed, 22 Jul 2020 15:07:57: 37000000 INFO @ Wed, 22 Jul 2020 15:07:58: 24000000 INFO @ Wed, 22 Jul 2020 15:08:01: 31000000 INFO @ Wed, 22 Jul 2020 15:08:02: 38000000 INFO @ Wed, 22 Jul 2020 15:08:02: 25000000 INFO @ Wed, 22 Jul 2020 15:08:06: 32000000 INFO @ Wed, 22 Jul 2020 15:08:07: 39000000 INFO @ Wed, 22 Jul 2020 15:08:07: 26000000 INFO @ Wed, 22 Jul 2020 15:08:10: 33000000 INFO @ Wed, 22 Jul 2020 15:08:11: 40000000 INFO @ Wed, 22 Jul 2020 15:08:12: 27000000 INFO @ Wed, 22 Jul 2020 15:08:15: 34000000 INFO @ Wed, 22 Jul 2020 15:08:16: 41000000 INFO @ Wed, 22 Jul 2020 15:08:16: 28000000 INFO @ Wed, 22 Jul 2020 15:08:20: 35000000 INFO @ Wed, 22 Jul 2020 15:08:21: 42000000 INFO @ Wed, 22 Jul 2020 15:08:21: 29000000 INFO @ Wed, 22 Jul 2020 15:08:24: 36000000 INFO @ Wed, 22 Jul 2020 15:08:25: 43000000 INFO @ Wed, 22 Jul 2020 15:08:26: 30000000 INFO @ Wed, 22 Jul 2020 15:08:29: 37000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Wed, 22 Jul 2020 15:08:30: 44000000 INFO @ Wed, 22 Jul 2020 15:08:31: 31000000 INFO @ Wed, 22 Jul 2020 15:08:34: 38000000 INFO @ Wed, 22 Jul 2020 15:08:35: 45000000 INFO @ Wed, 22 Jul 2020 15:08:35: 32000000 INFO @ Wed, 22 Jul 2020 15:08:39: 39000000 INFO @ Wed, 22 Jul 2020 15:08:39: 46000000 INFO @ Wed, 22 Jul 2020 15:08:40: 33000000 INFO @ Wed, 22 Jul 2020 15:08:43: 40000000 INFO @ Wed, 22 Jul 2020 15:08:44: 47000000 INFO @ Wed, 22 Jul 2020 15:08:45: 34000000 BigWig に変換しました。 INFO @ Wed, 22 Jul 2020 15:08:48: 41000000 INFO @ Wed, 22 Jul 2020 15:08:49: 48000000 INFO @ Wed, 22 Jul 2020 15:08:49: 35000000 INFO @ Wed, 22 Jul 2020 15:08:53: 42000000 INFO @ Wed, 22 Jul 2020 15:08:54: 49000000 INFO @ Wed, 22 Jul 2020 15:08:54: 36000000 INFO @ Wed, 22 Jul 2020 15:08:58: 43000000 INFO @ Wed, 22 Jul 2020 15:08:58: 50000000 INFO @ Wed, 22 Jul 2020 15:08:59: 37000000 INFO @ Wed, 22 Jul 2020 15:09:02: 44000000 INFO @ Wed, 22 Jul 2020 15:09:03: 51000000 INFO @ Wed, 22 Jul 2020 15:09:04: 38000000 INFO @ Wed, 22 Jul 2020 15:09:07: 45000000 INFO @ Wed, 22 Jul 2020 15:09:08: 52000000 INFO @ Wed, 22 Jul 2020 15:09:08: 39000000 INFO @ Wed, 22 Jul 2020 15:09:09: #1 tag size is determined as 51 bps INFO @ Wed, 22 Jul 2020 15:09:09: #1 tag size = 51 INFO @ Wed, 22 Jul 2020 15:09:09: #1 total tags in treatment: 25497326 INFO @ Wed, 22 Jul 2020 15:09:09: #1 user defined the maximum tags... INFO @ Wed, 22 Jul 2020 15:09:09: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 22 Jul 2020 15:09:09: #1 tags after filtering in treatment: 8327139 INFO @ Wed, 22 Jul 2020 15:09:09: #1 Redundant rate of treatment: 0.67 INFO @ Wed, 22 Jul 2020 15:09:09: #1 finished! INFO @ Wed, 22 Jul 2020 15:09:09: #2 Build Peak Model... INFO @ Wed, 22 Jul 2020 15:09:09: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 22 Jul 2020 15:09:10: #2 number of paired peaks: 0 WARNING @ Wed, 22 Jul 2020 15:09:10: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 22 Jul 2020 15:09:10: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8357692/SRX8357692.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8357692/SRX8357692.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8357692/SRX8357692.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8357692/SRX8357692.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Wed, 22 Jul 2020 15:09:12: 46000000 INFO @ Wed, 22 Jul 2020 15:09:13: 40000000 INFO @ Wed, 22 Jul 2020 15:09:17: 47000000 INFO @ Wed, 22 Jul 2020 15:09:18: 41000000 INFO @ Wed, 22 Jul 2020 15:09:21: 48000000 INFO @ Wed, 22 Jul 2020 15:09:22: 42000000 INFO @ Wed, 22 Jul 2020 15:09:26: 49000000 INFO @ Wed, 22 Jul 2020 15:09:27: 43000000 INFO @ Wed, 22 Jul 2020 15:09:31: 50000000 INFO @ Wed, 22 Jul 2020 15:09:32: 44000000 INFO @ Wed, 22 Jul 2020 15:09:35: 51000000 INFO @ Wed, 22 Jul 2020 15:09:36: 45000000 INFO @ Wed, 22 Jul 2020 15:09:40: 52000000 INFO @ Wed, 22 Jul 2020 15:09:41: 46000000 INFO @ Wed, 22 Jul 2020 15:09:41: #1 tag size is determined as 51 bps INFO @ Wed, 22 Jul 2020 15:09:41: #1 tag size = 51 INFO @ Wed, 22 Jul 2020 15:09:41: #1 total tags in treatment: 25497326 INFO @ Wed, 22 Jul 2020 15:09:41: #1 user defined the maximum tags... INFO @ Wed, 22 Jul 2020 15:09:41: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 22 Jul 2020 15:09:42: #1 tags after filtering in treatment: 8327139 INFO @ Wed, 22 Jul 2020 15:09:42: #1 Redundant rate of treatment: 0.67 INFO @ Wed, 22 Jul 2020 15:09:42: #1 finished! INFO @ Wed, 22 Jul 2020 15:09:42: #2 Build Peak Model... INFO @ Wed, 22 Jul 2020 15:09:42: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 22 Jul 2020 15:09:42: #2 number of paired peaks: 0 WARNING @ Wed, 22 Jul 2020 15:09:42: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 22 Jul 2020 15:09:42: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8357692/SRX8357692.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8357692/SRX8357692.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8357692/SRX8357692.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8357692/SRX8357692.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Wed, 22 Jul 2020 15:09:45: 47000000 INFO @ Wed, 22 Jul 2020 15:09:50: 48000000 INFO @ Wed, 22 Jul 2020 15:09:54: 49000000 INFO @ Wed, 22 Jul 2020 15:09:59: 50000000 INFO @ Wed, 22 Jul 2020 15:10:04: 51000000 INFO @ Wed, 22 Jul 2020 15:10:08: 52000000 INFO @ Wed, 22 Jul 2020 15:10:09: #1 tag size is determined as 51 bps INFO @ Wed, 22 Jul 2020 15:10:09: #1 tag size = 51 INFO @ Wed, 22 Jul 2020 15:10:09: #1 total tags in treatment: 25497326 INFO @ Wed, 22 Jul 2020 15:10:09: #1 user defined the maximum tags... INFO @ Wed, 22 Jul 2020 15:10:09: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 22 Jul 2020 15:10:10: #1 tags after filtering in treatment: 8327139 INFO @ Wed, 22 Jul 2020 15:10:10: #1 Redundant rate of treatment: 0.67 INFO @ Wed, 22 Jul 2020 15:10:10: #1 finished! INFO @ Wed, 22 Jul 2020 15:10:10: #2 Build Peak Model... INFO @ Wed, 22 Jul 2020 15:10:10: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 22 Jul 2020 15:10:10: #2 number of paired peaks: 0 WARNING @ Wed, 22 Jul 2020 15:10:10: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 22 Jul 2020 15:10:10: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8357692/SRX8357692.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8357692/SRX8357692.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8357692/SRX8357692.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8357692/SRX8357692.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling