Job ID = 7112747 SRX = SRX8357691 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... 2020-07-22T05:23:03 prefetch.2.10.7: 1) Downloading 'SRR11806279'... 2020-07-22T05:23:03 prefetch.2.10.7: Downloading via HTTPS... 2020-07-22T05:25:58 prefetch.2.10.7: HTTPS download succeed 2020-07-22T05:25:58 prefetch.2.10.7: 1) 'SRR11806279' was downloaded successfully 2020-07-22T05:25:58 prefetch.2.10.7: 'SRR11806279' has 0 unresolved dependencies Read 45170038 spots for SRR11806279/SRR11806279.sra Written 45170038 spots for SRR11806279/SRR11806279.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:23:48 45170038 reads; of these: 45170038 (100.00%) were paired; of these: 1968446 (4.36%) aligned concordantly 0 times 35559691 (78.72%) aligned concordantly exactly 1 time 7641901 (16.92%) aligned concordantly >1 times ---- 1968446 pairs aligned concordantly 0 times; of these: 351628 (17.86%) aligned discordantly 1 time ---- 1616818 pairs aligned 0 times concordantly or discordantly; of these: 3233636 mates make up the pairs; of these: 2431423 (75.19%) aligned 0 times 249008 (7.70%) aligned exactly 1 time 553205 (17.11%) aligned >1 times 97.31% overall alignment rate Time searching: 00:23:48 Overall time: 00:23:48 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 36 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 18007082 / 43422436 = 0.4147 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 22 Jul 2020 15:06:18: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8357691/SRX8357691.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8357691/SRX8357691.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8357691/SRX8357691.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8357691/SRX8357691.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 22 Jul 2020 15:06:18: #1 read tag files... INFO @ Wed, 22 Jul 2020 15:06:18: #1 read treatment tags... INFO @ Wed, 22 Jul 2020 15:06:24: 1000000 INFO @ Wed, 22 Jul 2020 15:06:30: 2000000 INFO @ Wed, 22 Jul 2020 15:06:36: 3000000 INFO @ Wed, 22 Jul 2020 15:06:42: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 22 Jul 2020 15:06:48: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8357691/SRX8357691.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8357691/SRX8357691.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8357691/SRX8357691.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8357691/SRX8357691.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 22 Jul 2020 15:06:48: #1 read tag files... INFO @ Wed, 22 Jul 2020 15:06:48: #1 read treatment tags... INFO @ Wed, 22 Jul 2020 15:06:48: 5000000 INFO @ Wed, 22 Jul 2020 15:06:55: 1000000 INFO @ Wed, 22 Jul 2020 15:06:55: 6000000 INFO @ Wed, 22 Jul 2020 15:07:01: 2000000 INFO @ Wed, 22 Jul 2020 15:07:02: 7000000 INFO @ Wed, 22 Jul 2020 15:07:07: 3000000 INFO @ Wed, 22 Jul 2020 15:07:09: 8000000 INFO @ Wed, 22 Jul 2020 15:07:14: 4000000 INFO @ Wed, 22 Jul 2020 15:07:16: 9000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 22 Jul 2020 15:07:18: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8357691/SRX8357691.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8357691/SRX8357691.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8357691/SRX8357691.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8357691/SRX8357691.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 22 Jul 2020 15:07:18: #1 read tag files... INFO @ Wed, 22 Jul 2020 15:07:18: #1 read treatment tags... INFO @ Wed, 22 Jul 2020 15:07:20: 5000000 INFO @ Wed, 22 Jul 2020 15:07:23: 10000000 INFO @ Wed, 22 Jul 2020 15:07:25: 1000000 INFO @ Wed, 22 Jul 2020 15:07:26: 6000000 INFO @ Wed, 22 Jul 2020 15:07:30: 11000000 INFO @ Wed, 22 Jul 2020 15:07:32: 2000000 INFO @ Wed, 22 Jul 2020 15:07:33: 7000000 INFO @ Wed, 22 Jul 2020 15:07:37: 12000000 INFO @ Wed, 22 Jul 2020 15:07:39: 3000000 INFO @ Wed, 22 Jul 2020 15:07:39: 8000000 INFO @ Wed, 22 Jul 2020 15:07:43: 13000000 INFO @ Wed, 22 Jul 2020 15:07:46: 9000000 INFO @ Wed, 22 Jul 2020 15:07:46: 4000000 INFO @ Wed, 22 Jul 2020 15:07:50: 14000000 INFO @ Wed, 22 Jul 2020 15:07:52: 10000000 INFO @ Wed, 22 Jul 2020 15:07:54: 5000000 INFO @ Wed, 22 Jul 2020 15:07:57: 15000000 INFO @ Wed, 22 Jul 2020 15:07:59: 11000000 INFO @ Wed, 22 Jul 2020 15:08:01: 6000000 INFO @ Wed, 22 Jul 2020 15:08:04: 16000000 INFO @ Wed, 22 Jul 2020 15:08:05: 12000000 INFO @ Wed, 22 Jul 2020 15:08:08: 7000000 INFO @ Wed, 22 Jul 2020 15:08:11: 17000000 INFO @ Wed, 22 Jul 2020 15:08:11: 13000000 INFO @ Wed, 22 Jul 2020 15:08:15: 8000000 INFO @ Wed, 22 Jul 2020 15:08:18: 14000000 INFO @ Wed, 22 Jul 2020 15:08:18: 18000000 INFO @ Wed, 22 Jul 2020 15:08:22: 9000000 INFO @ Wed, 22 Jul 2020 15:08:24: 15000000 INFO @ Wed, 22 Jul 2020 15:08:25: 19000000 INFO @ Wed, 22 Jul 2020 15:08:29: 10000000 INFO @ Wed, 22 Jul 2020 15:08:30: 16000000 INFO @ Wed, 22 Jul 2020 15:08:31: 20000000 INFO @ Wed, 22 Jul 2020 15:08:36: 11000000 INFO @ Wed, 22 Jul 2020 15:08:37: 17000000 INFO @ Wed, 22 Jul 2020 15:08:38: 21000000 INFO @ Wed, 22 Jul 2020 15:08:43: 12000000 INFO @ Wed, 22 Jul 2020 15:08:43: 18000000 INFO @ Wed, 22 Jul 2020 15:08:45: 22000000 INFO @ Wed, 22 Jul 2020 15:08:49: 19000000 INFO @ Wed, 22 Jul 2020 15:08:50: 13000000 INFO @ Wed, 22 Jul 2020 15:08:52: 23000000 INFO @ Wed, 22 Jul 2020 15:08:56: 20000000 INFO @ Wed, 22 Jul 2020 15:08:57: 14000000 INFO @ Wed, 22 Jul 2020 15:08:59: 24000000 INFO @ Wed, 22 Jul 2020 15:09:02: 21000000 INFO @ Wed, 22 Jul 2020 15:09:04: 15000000 INFO @ Wed, 22 Jul 2020 15:09:06: 25000000 INFO @ Wed, 22 Jul 2020 15:09:09: 22000000 INFO @ Wed, 22 Jul 2020 15:09:11: 16000000 INFO @ Wed, 22 Jul 2020 15:09:13: 26000000 INFO @ Wed, 22 Jul 2020 15:09:15: 23000000 INFO @ Wed, 22 Jul 2020 15:09:18: 17000000 INFO @ Wed, 22 Jul 2020 15:09:20: 27000000 INFO @ Wed, 22 Jul 2020 15:09:21: 24000000 INFO @ Wed, 22 Jul 2020 15:09:25: 18000000 INFO @ Wed, 22 Jul 2020 15:09:27: 28000000 INFO @ Wed, 22 Jul 2020 15:09:28: 25000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Wed, 22 Jul 2020 15:09:32: 19000000 INFO @ Wed, 22 Jul 2020 15:09:34: 29000000 INFO @ Wed, 22 Jul 2020 15:09:34: 26000000 INFO @ Wed, 22 Jul 2020 15:09:39: 20000000 INFO @ Wed, 22 Jul 2020 15:09:41: 27000000 INFO @ Wed, 22 Jul 2020 15:09:41: 30000000 BigWig に変換しました。 INFO @ Wed, 22 Jul 2020 15:09:46: 21000000 INFO @ Wed, 22 Jul 2020 15:09:47: 28000000 INFO @ Wed, 22 Jul 2020 15:09:48: 31000000 INFO @ Wed, 22 Jul 2020 15:09:53: 22000000 INFO @ Wed, 22 Jul 2020 15:09:54: 29000000 INFO @ Wed, 22 Jul 2020 15:09:55: 32000000 INFO @ Wed, 22 Jul 2020 15:10:00: 30000000 INFO @ Wed, 22 Jul 2020 15:10:00: 23000000 INFO @ Wed, 22 Jul 2020 15:10:02: 33000000 INFO @ Wed, 22 Jul 2020 15:10:06: 31000000 INFO @ Wed, 22 Jul 2020 15:10:07: 24000000 INFO @ Wed, 22 Jul 2020 15:10:09: 34000000 INFO @ Wed, 22 Jul 2020 15:10:13: 32000000 INFO @ Wed, 22 Jul 2020 15:10:14: 25000000 INFO @ Wed, 22 Jul 2020 15:10:16: 35000000 INFO @ Wed, 22 Jul 2020 15:10:19: 33000000 INFO @ Wed, 22 Jul 2020 15:10:21: 26000000 INFO @ Wed, 22 Jul 2020 15:10:23: 36000000 INFO @ Wed, 22 Jul 2020 15:10:25: 34000000 INFO @ Wed, 22 Jul 2020 15:10:28: 27000000 INFO @ Wed, 22 Jul 2020 15:10:30: 37000000 INFO @ Wed, 22 Jul 2020 15:10:32: 35000000 INFO @ Wed, 22 Jul 2020 15:10:35: 28000000 INFO @ Wed, 22 Jul 2020 15:10:37: 38000000 INFO @ Wed, 22 Jul 2020 15:10:38: 36000000 INFO @ Wed, 22 Jul 2020 15:10:42: 29000000 INFO @ Wed, 22 Jul 2020 15:10:44: 39000000 INFO @ Wed, 22 Jul 2020 15:10:45: 37000000 INFO @ Wed, 22 Jul 2020 15:10:50: 30000000 INFO @ Wed, 22 Jul 2020 15:10:51: 40000000 INFO @ Wed, 22 Jul 2020 15:10:51: 38000000 INFO @ Wed, 22 Jul 2020 15:10:57: 31000000 INFO @ Wed, 22 Jul 2020 15:10:57: 41000000 INFO @ Wed, 22 Jul 2020 15:10:58: 39000000 INFO @ Wed, 22 Jul 2020 15:11:04: 32000000 INFO @ Wed, 22 Jul 2020 15:11:04: 40000000 INFO @ Wed, 22 Jul 2020 15:11:04: 42000000 INFO @ Wed, 22 Jul 2020 15:11:10: 41000000 INFO @ Wed, 22 Jul 2020 15:11:11: 33000000 INFO @ Wed, 22 Jul 2020 15:11:11: 43000000 INFO @ Wed, 22 Jul 2020 15:11:16: 42000000 INFO @ Wed, 22 Jul 2020 15:11:18: 34000000 INFO @ Wed, 22 Jul 2020 15:11:18: 44000000 INFO @ Wed, 22 Jul 2020 15:11:23: 43000000 INFO @ Wed, 22 Jul 2020 15:11:25: 35000000 INFO @ Wed, 22 Jul 2020 15:11:25: 45000000 INFO @ Wed, 22 Jul 2020 15:11:29: 44000000 INFO @ Wed, 22 Jul 2020 15:11:32: 36000000 INFO @ Wed, 22 Jul 2020 15:11:32: 46000000 INFO @ Wed, 22 Jul 2020 15:11:35: 45000000 INFO @ Wed, 22 Jul 2020 15:11:39: 47000000 INFO @ Wed, 22 Jul 2020 15:11:39: 37000000 INFO @ Wed, 22 Jul 2020 15:11:42: 46000000 INFO @ Wed, 22 Jul 2020 15:11:46: 48000000 INFO @ Wed, 22 Jul 2020 15:11:46: 38000000 INFO @ Wed, 22 Jul 2020 15:11:48: 47000000 INFO @ Wed, 22 Jul 2020 15:11:53: 49000000 INFO @ Wed, 22 Jul 2020 15:11:53: 39000000 INFO @ Wed, 22 Jul 2020 15:11:55: 48000000 INFO @ Wed, 22 Jul 2020 15:11:59: 50000000 INFO @ Wed, 22 Jul 2020 15:12:00: 40000000 INFO @ Wed, 22 Jul 2020 15:12:01: 49000000 INFO @ Wed, 22 Jul 2020 15:12:06: 51000000 INFO @ Wed, 22 Jul 2020 15:12:07: 41000000 INFO @ Wed, 22 Jul 2020 15:12:07: 50000000 INFO @ Wed, 22 Jul 2020 15:12:12: #1 tag size is determined as 51 bps INFO @ Wed, 22 Jul 2020 15:12:12: #1 tag size = 51 INFO @ Wed, 22 Jul 2020 15:12:12: #1 total tags in treatment: 25282063 INFO @ Wed, 22 Jul 2020 15:12:12: #1 user defined the maximum tags... INFO @ Wed, 22 Jul 2020 15:12:12: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 22 Jul 2020 15:12:13: #1 tags after filtering in treatment: 8346241 INFO @ Wed, 22 Jul 2020 15:12:13: #1 Redundant rate of treatment: 0.67 INFO @ Wed, 22 Jul 2020 15:12:13: #1 finished! INFO @ Wed, 22 Jul 2020 15:12:13: #2 Build Peak Model... INFO @ Wed, 22 Jul 2020 15:12:13: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 22 Jul 2020 15:12:13: 51000000 INFO @ Wed, 22 Jul 2020 15:12:13: #2 number of paired peaks: 0 WARNING @ Wed, 22 Jul 2020 15:12:13: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 22 Jul 2020 15:12:13: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8357691/SRX8357691.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8357691/SRX8357691.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8357691/SRX8357691.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8357691/SRX8357691.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Wed, 22 Jul 2020 15:12:14: 42000000 INFO @ Wed, 22 Jul 2020 15:12:19: #1 tag size is determined as 51 bps INFO @ Wed, 22 Jul 2020 15:12:19: #1 tag size = 51 INFO @ Wed, 22 Jul 2020 15:12:19: #1 total tags in treatment: 25282063 INFO @ Wed, 22 Jul 2020 15:12:19: #1 user defined the maximum tags... INFO @ Wed, 22 Jul 2020 15:12:19: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 22 Jul 2020 15:12:20: #1 tags after filtering in treatment: 8346241 INFO @ Wed, 22 Jul 2020 15:12:20: #1 Redundant rate of treatment: 0.67 INFO @ Wed, 22 Jul 2020 15:12:20: #1 finished! INFO @ Wed, 22 Jul 2020 15:12:20: #2 Build Peak Model... INFO @ Wed, 22 Jul 2020 15:12:20: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 22 Jul 2020 15:12:20: #2 number of paired peaks: 0 WARNING @ Wed, 22 Jul 2020 15:12:20: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 22 Jul 2020 15:12:20: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8357691/SRX8357691.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8357691/SRX8357691.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8357691/SRX8357691.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8357691/SRX8357691.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Wed, 22 Jul 2020 15:12:21: 43000000 INFO @ Wed, 22 Jul 2020 15:12:27: 44000000 INFO @ Wed, 22 Jul 2020 15:12:33: 45000000 INFO @ Wed, 22 Jul 2020 15:12:39: 46000000 INFO @ Wed, 22 Jul 2020 15:12:45: 47000000 INFO @ Wed, 22 Jul 2020 15:12:51: 48000000 INFO @ Wed, 22 Jul 2020 15:12:57: 49000000 INFO @ Wed, 22 Jul 2020 15:13:03: 50000000 INFO @ Wed, 22 Jul 2020 15:13:09: 51000000 INFO @ Wed, 22 Jul 2020 15:13:15: #1 tag size is determined as 51 bps INFO @ Wed, 22 Jul 2020 15:13:15: #1 tag size = 51 INFO @ Wed, 22 Jul 2020 15:13:15: #1 total tags in treatment: 25282063 INFO @ Wed, 22 Jul 2020 15:13:15: #1 user defined the maximum tags... INFO @ Wed, 22 Jul 2020 15:13:15: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 22 Jul 2020 15:13:15: #1 tags after filtering in treatment: 8346241 INFO @ Wed, 22 Jul 2020 15:13:15: #1 Redundant rate of treatment: 0.67 INFO @ Wed, 22 Jul 2020 15:13:15: #1 finished! INFO @ Wed, 22 Jul 2020 15:13:15: #2 Build Peak Model... INFO @ Wed, 22 Jul 2020 15:13:15: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 22 Jul 2020 15:13:16: #2 number of paired peaks: 0 WARNING @ Wed, 22 Jul 2020 15:13:16: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 22 Jul 2020 15:13:16: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8357691/SRX8357691.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8357691/SRX8357691.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8357691/SRX8357691.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8357691/SRX8357691.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling