Job ID = 7112693 SRX = SRX8357688 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... 2020-07-22T05:22:18 prefetch.2.10.7: 1) Downloading 'SRR11806276'... 2020-07-22T05:22:18 prefetch.2.10.7: Downloading via HTTPS... 2020-07-22T05:24:27 prefetch.2.10.7: HTTPS download succeed 2020-07-22T05:24:27 prefetch.2.10.7: 1) 'SRR11806276' was downloaded successfully 2020-07-22T05:24:27 prefetch.2.10.7: 'SRR11806276' has 0 unresolved dependencies Read 33014990 spots for SRR11806276/SRR11806276.sra Written 33014990 spots for SRR11806276/SRR11806276.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:17:55 33014990 reads; of these: 33014990 (100.00%) were paired; of these: 2117082 (6.41%) aligned concordantly 0 times 26182222 (79.30%) aligned concordantly exactly 1 time 4715686 (14.28%) aligned concordantly >1 times ---- 2117082 pairs aligned concordantly 0 times; of these: 431722 (20.39%) aligned discordantly 1 time ---- 1685360 pairs aligned 0 times concordantly or discordantly; of these: 3370720 mates make up the pairs; of these: 2247934 (66.69%) aligned 0 times 268530 (7.97%) aligned exactly 1 time 854256 (25.34%) aligned >1 times 96.60% overall alignment rate Time searching: 00:17:55 Overall time: 00:17:55 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 28 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 12214052 / 31211908 = 0.3913 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 22 Jul 2020 14:54:54: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8357688/SRX8357688.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8357688/SRX8357688.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8357688/SRX8357688.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8357688/SRX8357688.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 22 Jul 2020 14:54:54: #1 read tag files... INFO @ Wed, 22 Jul 2020 14:54:54: #1 read treatment tags... INFO @ Wed, 22 Jul 2020 14:54:59: 1000000 INFO @ Wed, 22 Jul 2020 14:55:04: 2000000 INFO @ Wed, 22 Jul 2020 14:55:10: 3000000 INFO @ Wed, 22 Jul 2020 14:55:15: 4000000 INFO @ Wed, 22 Jul 2020 14:55:20: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 22 Jul 2020 14:55:24: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8357688/SRX8357688.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8357688/SRX8357688.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8357688/SRX8357688.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8357688/SRX8357688.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 22 Jul 2020 14:55:24: #1 read tag files... INFO @ Wed, 22 Jul 2020 14:55:24: #1 read treatment tags... INFO @ Wed, 22 Jul 2020 14:55:26: 6000000 INFO @ Wed, 22 Jul 2020 14:55:29: 1000000 INFO @ Wed, 22 Jul 2020 14:55:31: 7000000 INFO @ Wed, 22 Jul 2020 14:55:34: 2000000 INFO @ Wed, 22 Jul 2020 14:55:37: 8000000 INFO @ Wed, 22 Jul 2020 14:55:39: 3000000 INFO @ Wed, 22 Jul 2020 14:55:42: 9000000 INFO @ Wed, 22 Jul 2020 14:55:44: 4000000 INFO @ Wed, 22 Jul 2020 14:55:48: 10000000 INFO @ Wed, 22 Jul 2020 14:55:48: 5000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 22 Jul 2020 14:55:53: 11000000 INFO @ Wed, 22 Jul 2020 14:55:53: 6000000 INFO @ Wed, 22 Jul 2020 14:55:54: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8357688/SRX8357688.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8357688/SRX8357688.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8357688/SRX8357688.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8357688/SRX8357688.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 22 Jul 2020 14:55:54: #1 read tag files... INFO @ Wed, 22 Jul 2020 14:55:54: #1 read treatment tags... INFO @ Wed, 22 Jul 2020 14:55:58: 7000000 INFO @ Wed, 22 Jul 2020 14:55:59: 12000000 INFO @ Wed, 22 Jul 2020 14:55:59: 1000000 INFO @ Wed, 22 Jul 2020 14:56:03: 8000000 INFO @ Wed, 22 Jul 2020 14:56:04: 2000000 INFO @ Wed, 22 Jul 2020 14:56:04: 13000000 INFO @ Wed, 22 Jul 2020 14:56:08: 9000000 INFO @ Wed, 22 Jul 2020 14:56:09: 3000000 INFO @ Wed, 22 Jul 2020 14:56:10: 14000000 INFO @ Wed, 22 Jul 2020 14:56:13: 10000000 INFO @ Wed, 22 Jul 2020 14:56:14: 4000000 INFO @ Wed, 22 Jul 2020 14:56:15: 15000000 INFO @ Wed, 22 Jul 2020 14:56:18: 11000000 INFO @ Wed, 22 Jul 2020 14:56:18: 5000000 INFO @ Wed, 22 Jul 2020 14:56:21: 16000000 INFO @ Wed, 22 Jul 2020 14:56:23: 12000000 INFO @ Wed, 22 Jul 2020 14:56:23: 6000000 INFO @ Wed, 22 Jul 2020 14:56:27: 17000000 INFO @ Wed, 22 Jul 2020 14:56:28: 13000000 INFO @ Wed, 22 Jul 2020 14:56:28: 7000000 INFO @ Wed, 22 Jul 2020 14:56:32: 18000000 INFO @ Wed, 22 Jul 2020 14:56:33: 14000000 INFO @ Wed, 22 Jul 2020 14:56:33: 8000000 INFO @ Wed, 22 Jul 2020 14:56:38: 15000000 INFO @ Wed, 22 Jul 2020 14:56:38: 19000000 INFO @ Wed, 22 Jul 2020 14:56:38: 9000000 INFO @ Wed, 22 Jul 2020 14:56:42: 16000000 INFO @ Wed, 22 Jul 2020 14:56:43: 10000000 INFO @ Wed, 22 Jul 2020 14:56:43: 20000000 INFO @ Wed, 22 Jul 2020 14:56:47: 17000000 INFO @ Wed, 22 Jul 2020 14:56:47: 11000000 INFO @ Wed, 22 Jul 2020 14:56:49: 21000000 INFO @ Wed, 22 Jul 2020 14:56:52: 18000000 INFO @ Wed, 22 Jul 2020 14:56:52: 12000000 INFO @ Wed, 22 Jul 2020 14:56:55: 22000000 INFO @ Wed, 22 Jul 2020 14:56:57: 13000000 INFO @ Wed, 22 Jul 2020 14:56:57: 19000000 INFO @ Wed, 22 Jul 2020 14:57:00: 23000000 INFO @ Wed, 22 Jul 2020 14:57:02: 14000000 INFO @ Wed, 22 Jul 2020 14:57:02: 20000000 INFO @ Wed, 22 Jul 2020 14:57:06: 24000000 INFO @ Wed, 22 Jul 2020 14:57:07: 15000000 INFO @ Wed, 22 Jul 2020 14:57:07: 21000000 INFO @ Wed, 22 Jul 2020 14:57:11: 25000000 INFO @ Wed, 22 Jul 2020 14:57:12: 16000000 INFO @ Wed, 22 Jul 2020 14:57:12: 22000000 INFO @ Wed, 22 Jul 2020 14:57:17: 17000000 INFO @ Wed, 22 Jul 2020 14:57:17: 23000000 INFO @ Wed, 22 Jul 2020 14:57:17: 26000000 INFO @ Wed, 22 Jul 2020 14:57:22: 18000000 INFO @ Wed, 22 Jul 2020 14:57:22: 24000000 INFO @ Wed, 22 Jul 2020 14:57:23: 27000000 INFO @ Wed, 22 Jul 2020 14:57:27: 25000000 INFO @ Wed, 22 Jul 2020 14:57:27: 19000000 INFO @ Wed, 22 Jul 2020 14:57:28: 28000000 INFO @ Wed, 22 Jul 2020 14:57:31: 26000000 INFO @ Wed, 22 Jul 2020 14:57:32: 20000000 INFO @ Wed, 22 Jul 2020 14:57:34: 29000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Wed, 22 Jul 2020 14:57:36: 27000000 INFO @ Wed, 22 Jul 2020 14:57:37: 21000000 INFO @ Wed, 22 Jul 2020 14:57:39: 30000000 INFO @ Wed, 22 Jul 2020 14:57:41: 28000000 INFO @ Wed, 22 Jul 2020 14:57:41: 22000000 INFO @ Wed, 22 Jul 2020 14:57:45: 31000000 INFO @ Wed, 22 Jul 2020 14:57:46: 29000000 INFO @ Wed, 22 Jul 2020 14:57:46: 23000000 INFO @ Wed, 22 Jul 2020 14:57:50: 32000000 INFO @ Wed, 22 Jul 2020 14:57:51: 30000000 INFO @ Wed, 22 Jul 2020 14:57:51: 24000000 BigWig に変換しました。 INFO @ Wed, 22 Jul 2020 14:57:56: 33000000 INFO @ Wed, 22 Jul 2020 14:57:56: 31000000 INFO @ Wed, 22 Jul 2020 14:57:56: 25000000 INFO @ Wed, 22 Jul 2020 14:58:01: 32000000 INFO @ Wed, 22 Jul 2020 14:58:01: 26000000 INFO @ Wed, 22 Jul 2020 14:58:01: 34000000 INFO @ Wed, 22 Jul 2020 14:58:06: 33000000 INFO @ Wed, 22 Jul 2020 14:58:06: 27000000 INFO @ Wed, 22 Jul 2020 14:58:07: 35000000 INFO @ Wed, 22 Jul 2020 14:58:11: 34000000 INFO @ Wed, 22 Jul 2020 14:58:11: 28000000 INFO @ Wed, 22 Jul 2020 14:58:12: 36000000 INFO @ Wed, 22 Jul 2020 14:58:15: 35000000 INFO @ Wed, 22 Jul 2020 14:58:15: 29000000 INFO @ Wed, 22 Jul 2020 14:58:18: 37000000 INFO @ Wed, 22 Jul 2020 14:58:20: 36000000 INFO @ Wed, 22 Jul 2020 14:58:20: 30000000 INFO @ Wed, 22 Jul 2020 14:58:23: 38000000 INFO @ Wed, 22 Jul 2020 14:58:25: 31000000 INFO @ Wed, 22 Jul 2020 14:58:25: 37000000 INFO @ Wed, 22 Jul 2020 14:58:29: 39000000 INFO @ Wed, 22 Jul 2020 14:58:30: 32000000 INFO @ Wed, 22 Jul 2020 14:58:30: 38000000 INFO @ Wed, 22 Jul 2020 14:58:31: #1 tag size is determined as 51 bps INFO @ Wed, 22 Jul 2020 14:58:31: #1 tag size = 51 INFO @ Wed, 22 Jul 2020 14:58:31: #1 total tags in treatment: 18877654 INFO @ Wed, 22 Jul 2020 14:58:31: #1 user defined the maximum tags... INFO @ Wed, 22 Jul 2020 14:58:31: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 22 Jul 2020 14:58:31: #1 tags after filtering in treatment: 7245113 INFO @ Wed, 22 Jul 2020 14:58:31: #1 Redundant rate of treatment: 0.62 INFO @ Wed, 22 Jul 2020 14:58:31: #1 finished! INFO @ Wed, 22 Jul 2020 14:58:31: #2 Build Peak Model... INFO @ Wed, 22 Jul 2020 14:58:31: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 22 Jul 2020 14:58:32: #2 number of paired peaks: 0 WARNING @ Wed, 22 Jul 2020 14:58:32: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 22 Jul 2020 14:58:32: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8357688/SRX8357688.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8357688/SRX8357688.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8357688/SRX8357688.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8357688/SRX8357688.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Wed, 22 Jul 2020 14:58:35: 39000000 INFO @ Wed, 22 Jul 2020 14:58:35: 33000000 INFO @ Wed, 22 Jul 2020 14:58:36: #1 tag size is determined as 51 bps INFO @ Wed, 22 Jul 2020 14:58:36: #1 tag size = 51 INFO @ Wed, 22 Jul 2020 14:58:36: #1 total tags in treatment: 18877654 INFO @ Wed, 22 Jul 2020 14:58:36: #1 user defined the maximum tags... INFO @ Wed, 22 Jul 2020 14:58:36: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 22 Jul 2020 14:58:37: #1 tags after filtering in treatment: 7245113 INFO @ Wed, 22 Jul 2020 14:58:37: #1 Redundant rate of treatment: 0.62 INFO @ Wed, 22 Jul 2020 14:58:37: #1 finished! INFO @ Wed, 22 Jul 2020 14:58:37: #2 Build Peak Model... INFO @ Wed, 22 Jul 2020 14:58:37: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 22 Jul 2020 14:58:37: #2 number of paired peaks: 0 WARNING @ Wed, 22 Jul 2020 14:58:37: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 22 Jul 2020 14:58:37: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8357688/SRX8357688.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8357688/SRX8357688.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8357688/SRX8357688.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8357688/SRX8357688.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Wed, 22 Jul 2020 14:58:39: 34000000 INFO @ Wed, 22 Jul 2020 14:58:44: 35000000 INFO @ Wed, 22 Jul 2020 14:58:48: 36000000 INFO @ Wed, 22 Jul 2020 14:58:53: 37000000 INFO @ Wed, 22 Jul 2020 14:58:57: 38000000 INFO @ Wed, 22 Jul 2020 14:59:02: 39000000 INFO @ Wed, 22 Jul 2020 14:59:03: #1 tag size is determined as 51 bps INFO @ Wed, 22 Jul 2020 14:59:03: #1 tag size = 51 INFO @ Wed, 22 Jul 2020 14:59:03: #1 total tags in treatment: 18877654 INFO @ Wed, 22 Jul 2020 14:59:03: #1 user defined the maximum tags... INFO @ Wed, 22 Jul 2020 14:59:03: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 22 Jul 2020 14:59:04: #1 tags after filtering in treatment: 7245113 INFO @ Wed, 22 Jul 2020 14:59:04: #1 Redundant rate of treatment: 0.62 INFO @ Wed, 22 Jul 2020 14:59:04: #1 finished! INFO @ Wed, 22 Jul 2020 14:59:04: #2 Build Peak Model... INFO @ Wed, 22 Jul 2020 14:59:04: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 22 Jul 2020 14:59:04: #2 number of paired peaks: 0 WARNING @ Wed, 22 Jul 2020 14:59:04: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 22 Jul 2020 14:59:04: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8357688/SRX8357688.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8357688/SRX8357688.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8357688/SRX8357688.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8357688/SRX8357688.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling