Job ID = 7112165 SRX = SRX8357683 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... 2020-07-22T05:20:34 prefetch.2.10.7: 1) Downloading 'SRR11806271'... 2020-07-22T05:20:34 prefetch.2.10.7: Downloading via HTTPS... 2020-07-22T05:23:51 prefetch.2.10.7: HTTPS download succeed 2020-07-22T05:23:51 prefetch.2.10.7: 1) 'SRR11806271' was downloaded successfully 2020-07-22T05:23:51 prefetch.2.10.7: 'SRR11806271' has 0 unresolved dependencies Read 16834105 spots for SRR11806271/SRR11806271.sra Written 16834105 spots for SRR11806271/SRR11806271.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:19:32 16834105 reads; of these: 16834105 (100.00%) were paired; of these: 2583206 (15.35%) aligned concordantly 0 times 12158570 (72.23%) aligned concordantly exactly 1 time 2092329 (12.43%) aligned concordantly >1 times ---- 2583206 pairs aligned concordantly 0 times; of these: 1527599 (59.14%) aligned discordantly 1 time ---- 1055607 pairs aligned 0 times concordantly or discordantly; of these: 2111214 mates make up the pairs; of these: 1388156 (65.75%) aligned 0 times 170425 (8.07%) aligned exactly 1 time 552633 (26.18%) aligned >1 times 95.88% overall alignment rate Time searching: 00:19:32 Overall time: 00:19:32 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 24 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 2953622 / 15653278 = 0.1887 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 22 Jul 2020 14:55:48: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8357683/SRX8357683.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8357683/SRX8357683.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8357683/SRX8357683.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8357683/SRX8357683.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 22 Jul 2020 14:55:48: #1 read tag files... INFO @ Wed, 22 Jul 2020 14:55:48: #1 read treatment tags... INFO @ Wed, 22 Jul 2020 14:55:59: 1000000 INFO @ Wed, 22 Jul 2020 14:56:09: 2000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 22 Jul 2020 14:56:18: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8357683/SRX8357683.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8357683/SRX8357683.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8357683/SRX8357683.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8357683/SRX8357683.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 22 Jul 2020 14:56:18: #1 read tag files... INFO @ Wed, 22 Jul 2020 14:56:18: #1 read treatment tags... INFO @ Wed, 22 Jul 2020 14:56:19: 3000000 INFO @ Wed, 22 Jul 2020 14:56:29: 1000000 INFO @ Wed, 22 Jul 2020 14:56:30: 4000000 INFO @ Wed, 22 Jul 2020 14:56:39: 2000000 INFO @ Wed, 22 Jul 2020 14:56:41: 5000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 22 Jul 2020 14:56:49: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8357683/SRX8357683.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8357683/SRX8357683.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8357683/SRX8357683.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8357683/SRX8357683.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 22 Jul 2020 14:56:49: #1 read tag files... INFO @ Wed, 22 Jul 2020 14:56:49: #1 read treatment tags... INFO @ Wed, 22 Jul 2020 14:56:49: 3000000 INFO @ Wed, 22 Jul 2020 14:56:51: 6000000 INFO @ Wed, 22 Jul 2020 14:56:59: 1000000 INFO @ Wed, 22 Jul 2020 14:56:59: 4000000 INFO @ Wed, 22 Jul 2020 14:57:02: 7000000 INFO @ Wed, 22 Jul 2020 14:57:10: 2000000 INFO @ Wed, 22 Jul 2020 14:57:10: 5000000 INFO @ Wed, 22 Jul 2020 14:57:13: 8000000 INFO @ Wed, 22 Jul 2020 14:57:19: 3000000 INFO @ Wed, 22 Jul 2020 14:57:20: 6000000 INFO @ Wed, 22 Jul 2020 14:57:23: 9000000 INFO @ Wed, 22 Jul 2020 14:57:29: 4000000 INFO @ Wed, 22 Jul 2020 14:57:30: 7000000 INFO @ Wed, 22 Jul 2020 14:57:34: 10000000 INFO @ Wed, 22 Jul 2020 14:57:38: 5000000 INFO @ Wed, 22 Jul 2020 14:57:39: 8000000 INFO @ Wed, 22 Jul 2020 14:57:44: 11000000 INFO @ Wed, 22 Jul 2020 14:57:48: 6000000 INFO @ Wed, 22 Jul 2020 14:57:49: 9000000 INFO @ Wed, 22 Jul 2020 14:57:54: 12000000 INFO @ Wed, 22 Jul 2020 14:57:58: 7000000 INFO @ Wed, 22 Jul 2020 14:57:59: 10000000 INFO @ Wed, 22 Jul 2020 14:58:05: 13000000 INFO @ Wed, 22 Jul 2020 14:58:08: 8000000 INFO @ Wed, 22 Jul 2020 14:58:09: 11000000 INFO @ Wed, 22 Jul 2020 14:58:15: 14000000 INFO @ Wed, 22 Jul 2020 14:58:18: 9000000 INFO @ Wed, 22 Jul 2020 14:58:19: 12000000 INFO @ Wed, 22 Jul 2020 14:58:26: 15000000 INFO @ Wed, 22 Jul 2020 14:58:27: 10000000 INFO @ Wed, 22 Jul 2020 14:58:28: 13000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Wed, 22 Jul 2020 14:58:37: 11000000 INFO @ Wed, 22 Jul 2020 14:58:37: 16000000 INFO @ Wed, 22 Jul 2020 14:58:38: 14000000 BigWig に変換しました。 INFO @ Wed, 22 Jul 2020 14:58:46: 12000000 INFO @ Wed, 22 Jul 2020 14:58:48: 15000000 INFO @ Wed, 22 Jul 2020 14:58:48: 17000000 INFO @ Wed, 22 Jul 2020 14:58:56: 13000000 INFO @ Wed, 22 Jul 2020 14:58:58: 16000000 INFO @ Wed, 22 Jul 2020 14:58:59: 18000000 INFO @ Wed, 22 Jul 2020 14:59:06: 14000000 INFO @ Wed, 22 Jul 2020 14:59:07: 17000000 INFO @ Wed, 22 Jul 2020 14:59:10: 19000000 INFO @ Wed, 22 Jul 2020 14:59:15: 15000000 INFO @ Wed, 22 Jul 2020 14:59:17: 18000000 INFO @ Wed, 22 Jul 2020 14:59:21: 20000000 INFO @ Wed, 22 Jul 2020 14:59:25: 16000000 INFO @ Wed, 22 Jul 2020 14:59:27: 19000000 INFO @ Wed, 22 Jul 2020 14:59:32: 21000000 INFO @ Wed, 22 Jul 2020 14:59:35: 17000000 INFO @ Wed, 22 Jul 2020 14:59:37: 20000000 INFO @ Wed, 22 Jul 2020 14:59:44: 22000000 INFO @ Wed, 22 Jul 2020 14:59:44: 18000000 INFO @ Wed, 22 Jul 2020 14:59:46: 21000000 INFO @ Wed, 22 Jul 2020 14:59:54: 19000000 INFO @ Wed, 22 Jul 2020 14:59:55: 23000000 INFO @ Wed, 22 Jul 2020 14:59:56: 22000000 INFO @ Wed, 22 Jul 2020 15:00:03: 20000000 INFO @ Wed, 22 Jul 2020 15:00:06: 23000000 INFO @ Wed, 22 Jul 2020 15:00:06: 24000000 INFO @ Wed, 22 Jul 2020 15:00:13: 21000000 INFO @ Wed, 22 Jul 2020 15:00:16: 24000000 INFO @ Wed, 22 Jul 2020 15:00:17: 25000000 INFO @ Wed, 22 Jul 2020 15:00:23: 22000000 INFO @ Wed, 22 Jul 2020 15:00:26: 25000000 INFO @ Wed, 22 Jul 2020 15:00:29: 26000000 INFO @ Wed, 22 Jul 2020 15:00:33: 23000000 INFO @ Wed, 22 Jul 2020 15:00:33: #1 tag size is determined as 151 bps INFO @ Wed, 22 Jul 2020 15:00:33: #1 tag size = 151 INFO @ Wed, 22 Jul 2020 15:00:33: #1 total tags in treatment: 11463452 INFO @ Wed, 22 Jul 2020 15:00:33: #1 user defined the maximum tags... INFO @ Wed, 22 Jul 2020 15:00:33: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 22 Jul 2020 15:00:33: #1 tags after filtering in treatment: 5377841 INFO @ Wed, 22 Jul 2020 15:00:33: #1 Redundant rate of treatment: 0.53 INFO @ Wed, 22 Jul 2020 15:00:33: #1 finished! INFO @ Wed, 22 Jul 2020 15:00:33: #2 Build Peak Model... INFO @ Wed, 22 Jul 2020 15:00:33: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 22 Jul 2020 15:00:33: #2 number of paired peaks: 0 WARNING @ Wed, 22 Jul 2020 15:00:33: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 22 Jul 2020 15:00:33: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8357683/SRX8357683.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8357683/SRX8357683.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8357683/SRX8357683.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8357683/SRX8357683.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Wed, 22 Jul 2020 15:00:36: 26000000 INFO @ Wed, 22 Jul 2020 15:00:39: #1 tag size is determined as 151 bps INFO @ Wed, 22 Jul 2020 15:00:39: #1 tag size = 151 INFO @ Wed, 22 Jul 2020 15:00:39: #1 total tags in treatment: 11463452 INFO @ Wed, 22 Jul 2020 15:00:39: #1 user defined the maximum tags... INFO @ Wed, 22 Jul 2020 15:00:39: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 22 Jul 2020 15:00:39: #1 tags after filtering in treatment: 5377841 INFO @ Wed, 22 Jul 2020 15:00:39: #1 Redundant rate of treatment: 0.53 INFO @ Wed, 22 Jul 2020 15:00:39: #1 finished! INFO @ Wed, 22 Jul 2020 15:00:39: #2 Build Peak Model... INFO @ Wed, 22 Jul 2020 15:00:39: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 22 Jul 2020 15:00:40: #2 number of paired peaks: 0 WARNING @ Wed, 22 Jul 2020 15:00:40: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 22 Jul 2020 15:00:40: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8357683/SRX8357683.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8357683/SRX8357683.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8357683/SRX8357683.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8357683/SRX8357683.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Wed, 22 Jul 2020 15:00:42: 24000000 INFO @ Wed, 22 Jul 2020 15:00:50: 25000000 INFO @ Wed, 22 Jul 2020 15:00:58: 26000000 INFO @ Wed, 22 Jul 2020 15:01:01: #1 tag size is determined as 151 bps INFO @ Wed, 22 Jul 2020 15:01:01: #1 tag size = 151 INFO @ Wed, 22 Jul 2020 15:01:01: #1 total tags in treatment: 11463452 INFO @ Wed, 22 Jul 2020 15:01:01: #1 user defined the maximum tags... INFO @ Wed, 22 Jul 2020 15:01:01: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 22 Jul 2020 15:01:01: #1 tags after filtering in treatment: 5377841 INFO @ Wed, 22 Jul 2020 15:01:01: #1 Redundant rate of treatment: 0.53 INFO @ Wed, 22 Jul 2020 15:01:01: #1 finished! INFO @ Wed, 22 Jul 2020 15:01:01: #2 Build Peak Model... INFO @ Wed, 22 Jul 2020 15:01:01: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 22 Jul 2020 15:01:01: #2 number of paired peaks: 0 WARNING @ Wed, 22 Jul 2020 15:01:01: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 22 Jul 2020 15:01:01: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8357683/SRX8357683.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8357683/SRX8357683.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8357683/SRX8357683.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8357683/SRX8357683.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling