Job ID = 7112147 SRX = SRX8357681 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... 2020-07-22T05:19:19 prefetch.2.10.7: 1) Downloading 'SRR11806269'... 2020-07-22T05:19:19 prefetch.2.10.7: Downloading via HTTPS... 2020-07-22T05:23:45 prefetch.2.10.7: HTTPS download succeed 2020-07-22T05:23:45 prefetch.2.10.7: 1) 'SRR11806269' was downloaded successfully 2020-07-22T05:23:45 prefetch.2.10.7: 'SRR11806269' has 0 unresolved dependencies Read 20167708 spots for SRR11806269/SRR11806269.sra Written 20167708 spots for SRR11806269/SRR11806269.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:28:29 20167708 reads; of these: 20167708 (100.00%) were paired; of these: 2128696 (10.55%) aligned concordantly 0 times 16142768 (80.04%) aligned concordantly exactly 1 time 1896244 (9.40%) aligned concordantly >1 times ---- 2128696 pairs aligned concordantly 0 times; of these: 1289799 (60.59%) aligned discordantly 1 time ---- 838897 pairs aligned 0 times concordantly or discordantly; of these: 1677794 mates make up the pairs; of these: 1190952 (70.98%) aligned 0 times 167095 (9.96%) aligned exactly 1 time 319747 (19.06%) aligned >1 times 97.05% overall alignment rate Time searching: 00:28:29 Overall time: 00:28:29 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 32 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 3409590 / 19197780 = 0.1776 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 22 Jul 2020 15:07:41: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8357681/SRX8357681.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8357681/SRX8357681.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8357681/SRX8357681.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8357681/SRX8357681.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 22 Jul 2020 15:07:41: #1 read tag files... INFO @ Wed, 22 Jul 2020 15:07:41: #1 read treatment tags... INFO @ Wed, 22 Jul 2020 15:07:49: 1000000 INFO @ Wed, 22 Jul 2020 15:07:57: 2000000 INFO @ Wed, 22 Jul 2020 15:08:06: 3000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 22 Jul 2020 15:08:10: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8357681/SRX8357681.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8357681/SRX8357681.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8357681/SRX8357681.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8357681/SRX8357681.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 22 Jul 2020 15:08:10: #1 read tag files... INFO @ Wed, 22 Jul 2020 15:08:10: #1 read treatment tags... INFO @ Wed, 22 Jul 2020 15:08:14: 4000000 INFO @ Wed, 22 Jul 2020 15:08:20: 1000000 INFO @ Wed, 22 Jul 2020 15:08:24: 5000000 INFO @ Wed, 22 Jul 2020 15:08:29: 2000000 INFO @ Wed, 22 Jul 2020 15:08:33: 6000000 INFO @ Wed, 22 Jul 2020 15:08:38: 3000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 22 Jul 2020 15:08:40: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8357681/SRX8357681.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8357681/SRX8357681.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8357681/SRX8357681.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8357681/SRX8357681.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 22 Jul 2020 15:08:40: #1 read tag files... INFO @ Wed, 22 Jul 2020 15:08:40: #1 read treatment tags... INFO @ Wed, 22 Jul 2020 15:08:42: 7000000 INFO @ Wed, 22 Jul 2020 15:08:48: 4000000 INFO @ Wed, 22 Jul 2020 15:08:50: 1000000 INFO @ Wed, 22 Jul 2020 15:08:52: 8000000 INFO @ Wed, 22 Jul 2020 15:08:57: 5000000 INFO @ Wed, 22 Jul 2020 15:08:59: 2000000 INFO @ Wed, 22 Jul 2020 15:09:02: 9000000 INFO @ Wed, 22 Jul 2020 15:09:07: 6000000 INFO @ Wed, 22 Jul 2020 15:09:09: 3000000 INFO @ Wed, 22 Jul 2020 15:09:11: 10000000 INFO @ Wed, 22 Jul 2020 15:09:17: 7000000 INFO @ Wed, 22 Jul 2020 15:09:19: 4000000 INFO @ Wed, 22 Jul 2020 15:09:21: 11000000 INFO @ Wed, 22 Jul 2020 15:09:26: 8000000 INFO @ Wed, 22 Jul 2020 15:09:28: 5000000 INFO @ Wed, 22 Jul 2020 15:09:30: 12000000 INFO @ Wed, 22 Jul 2020 15:09:36: 9000000 INFO @ Wed, 22 Jul 2020 15:09:38: 6000000 INFO @ Wed, 22 Jul 2020 15:09:40: 13000000 INFO @ Wed, 22 Jul 2020 15:09:45: 10000000 INFO @ Wed, 22 Jul 2020 15:09:48: 7000000 INFO @ Wed, 22 Jul 2020 15:09:49: 14000000 INFO @ Wed, 22 Jul 2020 15:09:55: 11000000 INFO @ Wed, 22 Jul 2020 15:09:57: 8000000 INFO @ Wed, 22 Jul 2020 15:09:59: 15000000 INFO @ Wed, 22 Jul 2020 15:10:04: 12000000 INFO @ Wed, 22 Jul 2020 15:10:07: 9000000 INFO @ Wed, 22 Jul 2020 15:10:08: 16000000 INFO @ Wed, 22 Jul 2020 15:10:14: 13000000 INFO @ Wed, 22 Jul 2020 15:10:17: 10000000 INFO @ Wed, 22 Jul 2020 15:10:18: 17000000 INFO @ Wed, 22 Jul 2020 15:10:23: 14000000 INFO @ Wed, 22 Jul 2020 15:10:26: 11000000 INFO @ Wed, 22 Jul 2020 15:10:27: 18000000 INFO @ Wed, 22 Jul 2020 15:10:33: 15000000 INFO @ Wed, 22 Jul 2020 15:10:36: 12000000 INFO @ Wed, 22 Jul 2020 15:10:37: 19000000 INFO @ Wed, 22 Jul 2020 15:10:42: 16000000 INFO @ Wed, 22 Jul 2020 15:10:45: 13000000 INFO @ Wed, 22 Jul 2020 15:10:46: 20000000 INFO @ Wed, 22 Jul 2020 15:10:52: 17000000 INFO @ Wed, 22 Jul 2020 15:10:55: 14000000 INFO @ Wed, 22 Jul 2020 15:10:56: 21000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Wed, 22 Jul 2020 15:11:02: 18000000 INFO @ Wed, 22 Jul 2020 15:11:05: 15000000 INFO @ Wed, 22 Jul 2020 15:11:05: 22000000 INFO @ Wed, 22 Jul 2020 15:11:11: 19000000 BigWig に変換しました。 INFO @ Wed, 22 Jul 2020 15:11:15: 16000000 INFO @ Wed, 22 Jul 2020 15:11:15: 23000000 INFO @ Wed, 22 Jul 2020 15:11:21: 20000000 INFO @ Wed, 22 Jul 2020 15:11:24: 24000000 INFO @ Wed, 22 Jul 2020 15:11:25: 17000000 INFO @ Wed, 22 Jul 2020 15:11:31: 21000000 INFO @ Wed, 22 Jul 2020 15:11:34: 25000000 INFO @ Wed, 22 Jul 2020 15:11:35: 18000000 INFO @ Wed, 22 Jul 2020 15:11:40: 22000000 INFO @ Wed, 22 Jul 2020 15:11:43: 26000000 INFO @ Wed, 22 Jul 2020 15:11:44: 19000000 INFO @ Wed, 22 Jul 2020 15:11:50: 23000000 INFO @ Wed, 22 Jul 2020 15:11:52: 27000000 INFO @ Wed, 22 Jul 2020 15:11:54: 20000000 INFO @ Wed, 22 Jul 2020 15:12:00: 24000000 INFO @ Wed, 22 Jul 2020 15:12:01: 28000000 INFO @ Wed, 22 Jul 2020 15:12:03: 21000000 INFO @ Wed, 22 Jul 2020 15:12:09: 25000000 INFO @ Wed, 22 Jul 2020 15:12:11: 29000000 INFO @ Wed, 22 Jul 2020 15:12:13: 22000000 INFO @ Wed, 22 Jul 2020 15:12:19: 26000000 INFO @ Wed, 22 Jul 2020 15:12:20: 30000000 INFO @ Wed, 22 Jul 2020 15:12:23: 23000000 INFO @ Wed, 22 Jul 2020 15:12:28: 27000000 INFO @ Wed, 22 Jul 2020 15:12:29: 31000000 INFO @ Wed, 22 Jul 2020 15:12:32: 24000000 INFO @ Wed, 22 Jul 2020 15:12:37: 28000000 INFO @ Wed, 22 Jul 2020 15:12:38: 32000000 INFO @ Wed, 22 Jul 2020 15:12:41: #1 tag size is determined as 151 bps INFO @ Wed, 22 Jul 2020 15:12:41: #1 tag size = 151 INFO @ Wed, 22 Jul 2020 15:12:41: #1 total tags in treatment: 14760164 INFO @ Wed, 22 Jul 2020 15:12:41: #1 user defined the maximum tags... INFO @ Wed, 22 Jul 2020 15:12:41: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 22 Jul 2020 15:12:41: #1 tags after filtering in treatment: 6276627 INFO @ Wed, 22 Jul 2020 15:12:41: #1 Redundant rate of treatment: 0.57 INFO @ Wed, 22 Jul 2020 15:12:41: #1 finished! INFO @ Wed, 22 Jul 2020 15:12:41: #2 Build Peak Model... INFO @ Wed, 22 Jul 2020 15:12:41: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 22 Jul 2020 15:12:42: 25000000 INFO @ Wed, 22 Jul 2020 15:12:42: #2 number of paired peaks: 0 WARNING @ Wed, 22 Jul 2020 15:12:42: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 22 Jul 2020 15:12:42: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8357681/SRX8357681.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8357681/SRX8357681.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8357681/SRX8357681.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8357681/SRX8357681.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Wed, 22 Jul 2020 15:12:47: 29000000 INFO @ Wed, 22 Jul 2020 15:12:51: 26000000 INFO @ Wed, 22 Jul 2020 15:12:56: 30000000 INFO @ Wed, 22 Jul 2020 15:13:00: 27000000 INFO @ Wed, 22 Jul 2020 15:13:05: 31000000 INFO @ Wed, 22 Jul 2020 15:13:09: 28000000 INFO @ Wed, 22 Jul 2020 15:13:14: 32000000 INFO @ Wed, 22 Jul 2020 15:13:17: #1 tag size is determined as 151 bps INFO @ Wed, 22 Jul 2020 15:13:17: #1 tag size = 151 INFO @ Wed, 22 Jul 2020 15:13:17: #1 total tags in treatment: 14760164 INFO @ Wed, 22 Jul 2020 15:13:17: #1 user defined the maximum tags... INFO @ Wed, 22 Jul 2020 15:13:17: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 22 Jul 2020 15:13:17: #1 tags after filtering in treatment: 6276627 INFO @ Wed, 22 Jul 2020 15:13:17: #1 Redundant rate of treatment: 0.57 INFO @ Wed, 22 Jul 2020 15:13:17: #1 finished! INFO @ Wed, 22 Jul 2020 15:13:17: #2 Build Peak Model... INFO @ Wed, 22 Jul 2020 15:13:17: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 22 Jul 2020 15:13:18: #2 number of paired peaks: 0 WARNING @ Wed, 22 Jul 2020 15:13:18: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 22 Jul 2020 15:13:18: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8357681/SRX8357681.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8357681/SRX8357681.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8357681/SRX8357681.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8357681/SRX8357681.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Wed, 22 Jul 2020 15:13:18: 29000000 INFO @ Wed, 22 Jul 2020 15:13:26: 30000000 INFO @ Wed, 22 Jul 2020 15:13:34: 31000000 INFO @ Wed, 22 Jul 2020 15:13:43: 32000000 INFO @ Wed, 22 Jul 2020 15:13:45: #1 tag size is determined as 151 bps INFO @ Wed, 22 Jul 2020 15:13:45: #1 tag size = 151 INFO @ Wed, 22 Jul 2020 15:13:45: #1 total tags in treatment: 14760164 INFO @ Wed, 22 Jul 2020 15:13:45: #1 user defined the maximum tags... INFO @ Wed, 22 Jul 2020 15:13:45: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 22 Jul 2020 15:13:46: #1 tags after filtering in treatment: 6276627 INFO @ Wed, 22 Jul 2020 15:13:46: #1 Redundant rate of treatment: 0.57 INFO @ Wed, 22 Jul 2020 15:13:46: #1 finished! INFO @ Wed, 22 Jul 2020 15:13:46: #2 Build Peak Model... INFO @ Wed, 22 Jul 2020 15:13:46: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 22 Jul 2020 15:13:46: #2 number of paired peaks: 0 WARNING @ Wed, 22 Jul 2020 15:13:46: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 22 Jul 2020 15:13:46: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8357681/SRX8357681.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8357681/SRX8357681.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8357681/SRX8357681.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8357681/SRX8357681.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling