Job ID = 14520869 SRX = SRX8245548 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 27165641 spots for SRR11684759/SRR11684759.sra Written 27165641 spots for SRR11684759/SRR11684759.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:15:26 27165641 reads; of these: 27165641 (100.00%) were paired; of these: 14019560 (51.61%) aligned concordantly 0 times 11841913 (43.59%) aligned concordantly exactly 1 time 1304168 (4.80%) aligned concordantly >1 times ---- 14019560 pairs aligned concordantly 0 times; of these: 241830 (1.72%) aligned discordantly 1 time ---- 13777730 pairs aligned 0 times concordantly or discordantly; of these: 27555460 mates make up the pairs; of these: 16904255 (61.35%) aligned 0 times 9377649 (34.03%) aligned exactly 1 time 1273556 (4.62%) aligned >1 times 68.89% overall alignment rate Time searching: 00:15:26 Overall time: 00:15:26 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 16 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 2026962 / 13385741 = 0.1514 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 20:42:17: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8245548/SRX8245548.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8245548/SRX8245548.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8245548/SRX8245548.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8245548/SRX8245548.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 20:42:17: #1 read tag files... INFO @ Sat, 15 Jan 2022 20:42:17: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 20:42:22: 1000000 INFO @ Sat, 15 Jan 2022 20:42:27: 2000000 INFO @ Sat, 15 Jan 2022 20:42:32: 3000000 INFO @ Sat, 15 Jan 2022 20:42:37: 4000000 INFO @ Sat, 15 Jan 2022 20:42:42: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 20:42:47: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8245548/SRX8245548.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8245548/SRX8245548.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8245548/SRX8245548.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8245548/SRX8245548.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 20:42:47: #1 read tag files... INFO @ Sat, 15 Jan 2022 20:42:47: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 20:42:47: 6000000 INFO @ Sat, 15 Jan 2022 20:42:52: 1000000 INFO @ Sat, 15 Jan 2022 20:42:53: 7000000 INFO @ Sat, 15 Jan 2022 20:42:58: 2000000 INFO @ Sat, 15 Jan 2022 20:42:58: 8000000 INFO @ Sat, 15 Jan 2022 20:43:04: 9000000 INFO @ Sat, 15 Jan 2022 20:43:04: 3000000 INFO @ Sat, 15 Jan 2022 20:43:09: 10000000 INFO @ Sat, 15 Jan 2022 20:43:09: 4000000 INFO @ Sat, 15 Jan 2022 20:43:15: 11000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 20:43:15: 5000000 INFO @ Sat, 15 Jan 2022 20:43:17: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8245548/SRX8245548.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8245548/SRX8245548.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8245548/SRX8245548.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8245548/SRX8245548.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 20:43:17: #1 read tag files... INFO @ Sat, 15 Jan 2022 20:43:17: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 20:43:20: 12000000 INFO @ Sat, 15 Jan 2022 20:43:21: 6000000 INFO @ Sat, 15 Jan 2022 20:43:23: 1000000 INFO @ Sat, 15 Jan 2022 20:43:26: 13000000 INFO @ Sat, 15 Jan 2022 20:43:26: 7000000 INFO @ Sat, 15 Jan 2022 20:43:28: 2000000 INFO @ Sat, 15 Jan 2022 20:43:31: 14000000 INFO @ Sat, 15 Jan 2022 20:43:32: 8000000 INFO @ Sat, 15 Jan 2022 20:43:34: 3000000 INFO @ Sat, 15 Jan 2022 20:43:37: 15000000 INFO @ Sat, 15 Jan 2022 20:43:37: 9000000 INFO @ Sat, 15 Jan 2022 20:43:40: 4000000 INFO @ Sat, 15 Jan 2022 20:43:42: 16000000 INFO @ Sat, 15 Jan 2022 20:43:43: 10000000 INFO @ Sat, 15 Jan 2022 20:43:45: 5000000 INFO @ Sat, 15 Jan 2022 20:43:48: 17000000 INFO @ Sat, 15 Jan 2022 20:43:48: 11000000 INFO @ Sat, 15 Jan 2022 20:43:51: 6000000 INFO @ Sat, 15 Jan 2022 20:43:54: 12000000 INFO @ Sat, 15 Jan 2022 20:43:54: 18000000 INFO @ Sat, 15 Jan 2022 20:43:57: 7000000 INFO @ Sat, 15 Jan 2022 20:43:59: 13000000 INFO @ Sat, 15 Jan 2022 20:44:00: 19000000 INFO @ Sat, 15 Jan 2022 20:44:02: 8000000 INFO @ Sat, 15 Jan 2022 20:44:05: 14000000 INFO @ Sat, 15 Jan 2022 20:44:06: 20000000 INFO @ Sat, 15 Jan 2022 20:44:08: 9000000 INFO @ Sat, 15 Jan 2022 20:44:10: 15000000 INFO @ Sat, 15 Jan 2022 20:44:11: 21000000 INFO @ Sat, 15 Jan 2022 20:44:13: 10000000 INFO @ Sat, 15 Jan 2022 20:44:16: 16000000 INFO @ Sat, 15 Jan 2022 20:44:17: 22000000 INFO @ Sat, 15 Jan 2022 20:44:19: 11000000 INFO @ Sat, 15 Jan 2022 20:44:22: 17000000 INFO @ Sat, 15 Jan 2022 20:44:23: 23000000 INFO @ Sat, 15 Jan 2022 20:44:25: 12000000 INFO @ Sat, 15 Jan 2022 20:44:27: 18000000 INFO @ Sat, 15 Jan 2022 20:44:29: 24000000 INFO @ Sat, 15 Jan 2022 20:44:30: 13000000 INFO @ Sat, 15 Jan 2022 20:44:33: 19000000 INFO @ Sat, 15 Jan 2022 20:44:34: 25000000 INFO @ Sat, 15 Jan 2022 20:44:36: 14000000 INFO @ Sat, 15 Jan 2022 20:44:39: 20000000 INFO @ Sat, 15 Jan 2022 20:44:40: 26000000 INFO @ Sat, 15 Jan 2022 20:44:41: 15000000 INFO @ Sat, 15 Jan 2022 20:44:45: 21000000 INFO @ Sat, 15 Jan 2022 20:44:46: 27000000 INFO @ Sat, 15 Jan 2022 20:44:47: 16000000 INFO @ Sat, 15 Jan 2022 20:44:51: 22000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 20:44:52: 28000000 INFO @ Sat, 15 Jan 2022 20:44:53: 17000000 INFO @ Sat, 15 Jan 2022 20:44:56: 23000000 INFO @ Sat, 15 Jan 2022 20:44:58: 29000000 INFO @ Sat, 15 Jan 2022 20:44:58: 18000000 INFO @ Sat, 15 Jan 2022 20:45:02: 24000000 INFO @ Sat, 15 Jan 2022 20:45:04: 30000000 INFO @ Sat, 15 Jan 2022 20:45:04: 19000000 BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 20:45:08: 25000000 INFO @ Sat, 15 Jan 2022 20:45:09: 31000000 INFO @ Sat, 15 Jan 2022 20:45:10: 20000000 INFO @ Sat, 15 Jan 2022 20:45:14: 26000000 INFO @ Sat, 15 Jan 2022 20:45:15: 32000000 INFO @ Sat, 15 Jan 2022 20:45:16: 21000000 INFO @ Sat, 15 Jan 2022 20:45:20: 27000000 INFO @ Sat, 15 Jan 2022 20:45:21: 33000000 INFO @ Sat, 15 Jan 2022 20:45:21: 22000000 INFO @ Sat, 15 Jan 2022 20:45:23: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 20:45:23: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 20:45:23: #1 total tags in treatment: 11134655 INFO @ Sat, 15 Jan 2022 20:45:23: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 20:45:23: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 20:45:23: #1 tags after filtering in treatment: 6441826 INFO @ Sat, 15 Jan 2022 20:45:23: #1 Redundant rate of treatment: 0.42 INFO @ Sat, 15 Jan 2022 20:45:23: #1 finished! INFO @ Sat, 15 Jan 2022 20:45:23: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 20:45:23: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 20:45:24: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 20:45:24: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 20:45:24: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8245548/SRX8245548.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245548/SRX8245548.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245548/SRX8245548.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245548/SRX8245548.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 20:45:25: 28000000 INFO @ Sat, 15 Jan 2022 20:45:27: 23000000 INFO @ Sat, 15 Jan 2022 20:45:31: 29000000 INFO @ Sat, 15 Jan 2022 20:45:33: 24000000 INFO @ Sat, 15 Jan 2022 20:45:37: 30000000 INFO @ Sat, 15 Jan 2022 20:45:38: 25000000 INFO @ Sat, 15 Jan 2022 20:45:42: 31000000 INFO @ Sat, 15 Jan 2022 20:45:44: 26000000 INFO @ Sat, 15 Jan 2022 20:45:48: 32000000 INFO @ Sat, 15 Jan 2022 20:45:49: 27000000 INFO @ Sat, 15 Jan 2022 20:45:54: 33000000 INFO @ Sat, 15 Jan 2022 20:45:55: 28000000 INFO @ Sat, 15 Jan 2022 20:45:56: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 20:45:56: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 20:45:56: #1 total tags in treatment: 11134655 INFO @ Sat, 15 Jan 2022 20:45:56: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 20:45:56: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 20:45:56: #1 tags after filtering in treatment: 6441826 INFO @ Sat, 15 Jan 2022 20:45:56: #1 Redundant rate of treatment: 0.42 INFO @ Sat, 15 Jan 2022 20:45:56: #1 finished! INFO @ Sat, 15 Jan 2022 20:45:56: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 20:45:56: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 20:45:56: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 20:45:56: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 20:45:56: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8245548/SRX8245548.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245548/SRX8245548.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245548/SRX8245548.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245548/SRX8245548.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 20:46:00: 29000000 INFO @ Sat, 15 Jan 2022 20:46:06: 30000000 INFO @ Sat, 15 Jan 2022 20:46:11: 31000000 INFO @ Sat, 15 Jan 2022 20:46:16: 32000000 INFO @ Sat, 15 Jan 2022 20:46:21: 33000000 INFO @ Sat, 15 Jan 2022 20:46:23: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 20:46:23: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 20:46:23: #1 total tags in treatment: 11134655 INFO @ Sat, 15 Jan 2022 20:46:23: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 20:46:23: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 20:46:24: #1 tags after filtering in treatment: 6441826 INFO @ Sat, 15 Jan 2022 20:46:24: #1 Redundant rate of treatment: 0.42 INFO @ Sat, 15 Jan 2022 20:46:24: #1 finished! INFO @ Sat, 15 Jan 2022 20:46:24: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 20:46:24: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 20:46:24: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 20:46:24: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 20:46:24: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8245548/SRX8245548.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245548/SRX8245548.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245548/SRX8245548.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245548/SRX8245548.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling