Job ID = 14520746 SRX = SRX8245507 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 25609018 spots for SRR11684718/SRR11684718.sra Written 25609018 spots for SRR11684718/SRR11684718.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:52:22 25609018 reads; of these: 25609018 (100.00%) were paired; of these: 9051653 (35.35%) aligned concordantly 0 times 10003920 (39.06%) aligned concordantly exactly 1 time 6553445 (25.59%) aligned concordantly >1 times ---- 9051653 pairs aligned concordantly 0 times; of these: 430863 (4.76%) aligned discordantly 1 time ---- 8620790 pairs aligned 0 times concordantly or discordantly; of these: 17241580 mates make up the pairs; of these: 11007084 (63.84%) aligned 0 times 3662449 (21.24%) aligned exactly 1 time 2572047 (14.92%) aligned >1 times 78.51% overall alignment rate Time searching: 00:52:22 Overall time: 00:52:22 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 20 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 2244521 / 16985506 = 0.1321 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 20:57:40: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8245507/SRX8245507.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8245507/SRX8245507.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8245507/SRX8245507.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8245507/SRX8245507.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 20:57:40: #1 read tag files... INFO @ Sat, 15 Jan 2022 20:57:40: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 20:57:52: 1000000 INFO @ Sat, 15 Jan 2022 20:58:04: 2000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 20:58:10: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8245507/SRX8245507.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8245507/SRX8245507.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8245507/SRX8245507.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8245507/SRX8245507.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 20:58:10: #1 read tag files... INFO @ Sat, 15 Jan 2022 20:58:10: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 20:58:18: 3000000 INFO @ Sat, 15 Jan 2022 20:58:24: 1000000 INFO @ Sat, 15 Jan 2022 20:58:31: 4000000 INFO @ Sat, 15 Jan 2022 20:58:37: 2000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 20:58:40: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8245507/SRX8245507.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8245507/SRX8245507.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8245507/SRX8245507.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8245507/SRX8245507.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 20:58:40: #1 read tag files... INFO @ Sat, 15 Jan 2022 20:58:40: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 20:58:45: 5000000 INFO @ Sat, 15 Jan 2022 20:58:51: 1000000 INFO @ Sat, 15 Jan 2022 20:58:52: 3000000 INFO @ Sat, 15 Jan 2022 20:59:00: 6000000 INFO @ Sat, 15 Jan 2022 20:59:03: 2000000 INFO @ Sat, 15 Jan 2022 20:59:07: 4000000 INFO @ Sat, 15 Jan 2022 20:59:13: 3000000 INFO @ Sat, 15 Jan 2022 20:59:15: 7000000 INFO @ Sat, 15 Jan 2022 20:59:21: 5000000 INFO @ Sat, 15 Jan 2022 20:59:23: 4000000 INFO @ Sat, 15 Jan 2022 20:59:30: 8000000 INFO @ Sat, 15 Jan 2022 20:59:33: 5000000 INFO @ Sat, 15 Jan 2022 20:59:36: 6000000 INFO @ Sat, 15 Jan 2022 20:59:44: 9000000 INFO @ Sat, 15 Jan 2022 20:59:44: 6000000 INFO @ Sat, 15 Jan 2022 20:59:51: 7000000 INFO @ Sat, 15 Jan 2022 20:59:55: 7000000 INFO @ Sat, 15 Jan 2022 20:59:58: 10000000 INFO @ Sat, 15 Jan 2022 21:00:05: 8000000 INFO @ Sat, 15 Jan 2022 21:00:05: 8000000 INFO @ Sat, 15 Jan 2022 21:00:12: 11000000 INFO @ Sat, 15 Jan 2022 21:00:15: 9000000 INFO @ Sat, 15 Jan 2022 21:00:20: 9000000 INFO @ Sat, 15 Jan 2022 21:00:26: 10000000 INFO @ Sat, 15 Jan 2022 21:00:26: 12000000 INFO @ Sat, 15 Jan 2022 21:00:34: 10000000 INFO @ Sat, 15 Jan 2022 21:00:36: 11000000 INFO @ Sat, 15 Jan 2022 21:00:39: 13000000 INFO @ Sat, 15 Jan 2022 21:00:46: 12000000 INFO @ Sat, 15 Jan 2022 21:00:48: 11000000 INFO @ Sat, 15 Jan 2022 21:00:52: 14000000 INFO @ Sat, 15 Jan 2022 21:00:57: 13000000 INFO @ Sat, 15 Jan 2022 21:01:02: 12000000 INFO @ Sat, 15 Jan 2022 21:01:06: 15000000 INFO @ Sat, 15 Jan 2022 21:01:06: 14000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 21:01:16: 13000000 INFO @ Sat, 15 Jan 2022 21:01:16: 15000000 INFO @ Sat, 15 Jan 2022 21:01:20: 16000000 INFO @ Sat, 15 Jan 2022 21:01:26: 16000000 BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 21:01:29: 14000000 INFO @ Sat, 15 Jan 2022 21:01:32: 17000000 INFO @ Sat, 15 Jan 2022 21:01:36: 17000000 INFO @ Sat, 15 Jan 2022 21:01:43: 15000000 INFO @ Sat, 15 Jan 2022 21:01:45: 18000000 INFO @ Sat, 15 Jan 2022 21:01:46: 18000000 INFO @ Sat, 15 Jan 2022 21:01:56: 19000000 INFO @ Sat, 15 Jan 2022 21:01:57: 16000000 INFO @ Sat, 15 Jan 2022 21:01:59: 19000000 INFO @ Sat, 15 Jan 2022 21:02:06: 20000000 INFO @ Sat, 15 Jan 2022 21:02:11: 17000000 INFO @ Sat, 15 Jan 2022 21:02:13: 20000000 INFO @ Sat, 15 Jan 2022 21:02:16: 21000000 INFO @ Sat, 15 Jan 2022 21:02:26: 18000000 INFO @ Sat, 15 Jan 2022 21:02:26: 22000000 INFO @ Sat, 15 Jan 2022 21:02:28: 21000000 INFO @ Sat, 15 Jan 2022 21:02:37: 23000000 INFO @ Sat, 15 Jan 2022 21:02:40: 19000000 INFO @ Sat, 15 Jan 2022 21:02:42: 22000000 INFO @ Sat, 15 Jan 2022 21:02:47: 24000000 INFO @ Sat, 15 Jan 2022 21:02:54: 20000000 INFO @ Sat, 15 Jan 2022 21:02:56: 23000000 INFO @ Sat, 15 Jan 2022 21:02:57: 25000000 INFO @ Sat, 15 Jan 2022 21:03:07: 21000000 INFO @ Sat, 15 Jan 2022 21:03:08: 26000000 INFO @ Sat, 15 Jan 2022 21:03:10: 24000000 INFO @ Sat, 15 Jan 2022 21:03:18: 27000000 INFO @ Sat, 15 Jan 2022 21:03:20: 22000000 INFO @ Sat, 15 Jan 2022 21:03:24: 25000000 INFO @ Sat, 15 Jan 2022 21:03:28: 28000000 INFO @ Sat, 15 Jan 2022 21:03:33: 23000000 INFO @ Sat, 15 Jan 2022 21:03:37: 29000000 INFO @ Sat, 15 Jan 2022 21:03:38: 26000000 INFO @ Sat, 15 Jan 2022 21:03:46: 24000000 INFO @ Sat, 15 Jan 2022 21:03:47: 30000000 INFO @ Sat, 15 Jan 2022 21:03:51: 27000000 INFO @ Sat, 15 Jan 2022 21:03:57: 31000000 INFO @ Sat, 15 Jan 2022 21:03:59: 25000000 INFO @ Sat, 15 Jan 2022 21:04:05: 28000000 INFO @ Sat, 15 Jan 2022 21:04:06: 32000000 INFO @ Sat, 15 Jan 2022 21:04:13: 26000000 INFO @ Sat, 15 Jan 2022 21:04:16: 33000000 INFO @ Sat, 15 Jan 2022 21:04:19: 29000000 INFO @ Sat, 15 Jan 2022 21:04:25: 34000000 INFO @ Sat, 15 Jan 2022 21:04:27: 27000000 INFO @ Sat, 15 Jan 2022 21:04:32: 30000000 INFO @ Sat, 15 Jan 2022 21:04:34: 35000000 INFO @ Sat, 15 Jan 2022 21:04:41: 28000000 INFO @ Sat, 15 Jan 2022 21:04:41: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 21:04:41: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 21:04:41: #1 total tags in treatment: 14333573 INFO @ Sat, 15 Jan 2022 21:04:41: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 21:04:41: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 21:04:42: #1 tags after filtering in treatment: 6374616 INFO @ Sat, 15 Jan 2022 21:04:42: #1 Redundant rate of treatment: 0.56 INFO @ Sat, 15 Jan 2022 21:04:42: #1 finished! INFO @ Sat, 15 Jan 2022 21:04:42: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 21:04:42: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 21:04:42: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 21:04:42: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 21:04:42: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8245507/SRX8245507.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245507/SRX8245507.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245507/SRX8245507.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245507/SRX8245507.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 21:04:46: 31000000 INFO @ Sat, 15 Jan 2022 21:04:55: 29000000 INFO @ Sat, 15 Jan 2022 21:05:00: 32000000 INFO @ Sat, 15 Jan 2022 21:05:07: 30000000 INFO @ Sat, 15 Jan 2022 21:05:13: 33000000 INFO @ Sat, 15 Jan 2022 21:05:19: 31000000 INFO @ Sat, 15 Jan 2022 21:05:24: 34000000 INFO @ Sat, 15 Jan 2022 21:05:31: 32000000 INFO @ Sat, 15 Jan 2022 21:05:36: 35000000 INFO @ Sat, 15 Jan 2022 21:05:42: 33000000 INFO @ Sat, 15 Jan 2022 21:05:44: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 21:05:44: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 21:05:44: #1 total tags in treatment: 14333573 INFO @ Sat, 15 Jan 2022 21:05:44: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 21:05:44: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 21:05:44: #1 tags after filtering in treatment: 6374616 INFO @ Sat, 15 Jan 2022 21:05:44: #1 Redundant rate of treatment: 0.56 INFO @ Sat, 15 Jan 2022 21:05:44: #1 finished! INFO @ Sat, 15 Jan 2022 21:05:44: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 21:05:44: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 21:05:45: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 21:05:45: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 21:05:45: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8245507/SRX8245507.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245507/SRX8245507.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245507/SRX8245507.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245507/SRX8245507.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 21:05:52: 34000000 INFO @ Sat, 15 Jan 2022 21:06:02: 35000000 INFO @ Sat, 15 Jan 2022 21:06:09: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 21:06:09: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 21:06:09: #1 total tags in treatment: 14333573 INFO @ Sat, 15 Jan 2022 21:06:09: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 21:06:09: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 21:06:09: #1 tags after filtering in treatment: 6374616 INFO @ Sat, 15 Jan 2022 21:06:09: #1 Redundant rate of treatment: 0.56 INFO @ Sat, 15 Jan 2022 21:06:09: #1 finished! INFO @ Sat, 15 Jan 2022 21:06:09: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 21:06:09: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 21:06:10: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 21:06:10: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 21:06:10: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8245507/SRX8245507.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245507/SRX8245507.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245507/SRX8245507.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245507/SRX8245507.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling