Job ID = 14521210 SRX = SRX8245498 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 80064243 spots for SRR11684709/SRR11684709.sra Written 80064243 spots for SRR11684709/SRR11684709.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:21:08 80064243 reads; of these: 80064243 (100.00%) were paired; of these: 68996250 (86.18%) aligned concordantly 0 times 9134339 (11.41%) aligned concordantly exactly 1 time 1933654 (2.42%) aligned concordantly >1 times ---- 68996250 pairs aligned concordantly 0 times; of these: 210773 (0.31%) aligned discordantly 1 time ---- 68785477 pairs aligned 0 times concordantly or discordantly; of these: 137570954 mates make up the pairs; of these: 132675875 (96.44%) aligned 0 times 3839327 (2.79%) aligned exactly 1 time 1055752 (0.77%) aligned >1 times 17.14% overall alignment rate Time searching: 00:21:08 Overall time: 00:21:08 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 12 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 1341585 / 11267979 = 0.1191 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 21:56:19: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8245498/SRX8245498.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8245498/SRX8245498.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8245498/SRX8245498.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8245498/SRX8245498.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 21:56:19: #1 read tag files... INFO @ Sat, 15 Jan 2022 21:56:19: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 21:56:27: 1000000 INFO @ Sat, 15 Jan 2022 21:56:35: 2000000 INFO @ Sat, 15 Jan 2022 21:56:43: 3000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 21:56:49: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8245498/SRX8245498.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8245498/SRX8245498.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8245498/SRX8245498.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8245498/SRX8245498.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 21:56:49: #1 read tag files... INFO @ Sat, 15 Jan 2022 21:56:49: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 21:56:51: 4000000 INFO @ Sat, 15 Jan 2022 21:57:00: 1000000 INFO @ Sat, 15 Jan 2022 21:57:00: 5000000 INFO @ Sat, 15 Jan 2022 21:57:09: 6000000 INFO @ Sat, 15 Jan 2022 21:57:10: 2000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 21:57:17: 7000000 INFO @ Sat, 15 Jan 2022 21:57:19: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8245498/SRX8245498.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8245498/SRX8245498.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8245498/SRX8245498.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8245498/SRX8245498.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 21:57:19: #1 read tag files... INFO @ Sat, 15 Jan 2022 21:57:19: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 21:57:20: 3000000 INFO @ Sat, 15 Jan 2022 21:57:26: 8000000 INFO @ Sat, 15 Jan 2022 21:57:28: 1000000 INFO @ Sat, 15 Jan 2022 21:57:31: 4000000 INFO @ Sat, 15 Jan 2022 21:57:35: 9000000 INFO @ Sat, 15 Jan 2022 21:57:37: 2000000 INFO @ Sat, 15 Jan 2022 21:57:41: 5000000 INFO @ Sat, 15 Jan 2022 21:57:44: 10000000 INFO @ Sat, 15 Jan 2022 21:57:46: 3000000 INFO @ Sat, 15 Jan 2022 21:57:52: 6000000 INFO @ Sat, 15 Jan 2022 21:57:53: 11000000 INFO @ Sat, 15 Jan 2022 21:57:55: 4000000 INFO @ Sat, 15 Jan 2022 21:58:02: 7000000 INFO @ Sat, 15 Jan 2022 21:58:02: 12000000 INFO @ Sat, 15 Jan 2022 21:58:04: 5000000 INFO @ Sat, 15 Jan 2022 21:58:11: 13000000 INFO @ Sat, 15 Jan 2022 21:58:13: 6000000 INFO @ Sat, 15 Jan 2022 21:58:13: 8000000 INFO @ Sat, 15 Jan 2022 21:58:21: 14000000 INFO @ Sat, 15 Jan 2022 21:58:22: 7000000 INFO @ Sat, 15 Jan 2022 21:58:23: 9000000 INFO @ Sat, 15 Jan 2022 21:58:29: 15000000 INFO @ Sat, 15 Jan 2022 21:58:31: 8000000 INFO @ Sat, 15 Jan 2022 21:58:34: 10000000 INFO @ Sat, 15 Jan 2022 21:58:38: 16000000 INFO @ Sat, 15 Jan 2022 21:58:39: 9000000 INFO @ Sat, 15 Jan 2022 21:58:44: 11000000 INFO @ Sat, 15 Jan 2022 21:58:47: 17000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 21:58:48: 10000000 INFO @ Sat, 15 Jan 2022 21:58:55: 12000000 INFO @ Sat, 15 Jan 2022 21:58:56: 18000000 INFO @ Sat, 15 Jan 2022 21:58:57: 11000000 BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 21:59:05: 19000000 INFO @ Sat, 15 Jan 2022 21:59:05: 13000000 INFO @ Sat, 15 Jan 2022 21:59:06: 12000000 INFO @ Sat, 15 Jan 2022 21:59:14: 20000000 INFO @ Sat, 15 Jan 2022 21:59:15: 13000000 INFO @ Sat, 15 Jan 2022 21:59:16: 14000000 INFO @ Sat, 15 Jan 2022 21:59:23: 21000000 INFO @ Sat, 15 Jan 2022 21:59:24: 14000000 INFO @ Sat, 15 Jan 2022 21:59:27: 15000000 INFO @ Sat, 15 Jan 2022 21:59:32: 22000000 INFO @ Sat, 15 Jan 2022 21:59:33: 15000000 INFO @ Sat, 15 Jan 2022 21:59:38: 16000000 INFO @ Sat, 15 Jan 2022 21:59:41: 23000000 INFO @ Sat, 15 Jan 2022 21:59:42: 16000000 INFO @ Sat, 15 Jan 2022 21:59:48: 17000000 INFO @ Sat, 15 Jan 2022 21:59:50: 24000000 INFO @ Sat, 15 Jan 2022 21:59:52: 17000000 INFO @ Sat, 15 Jan 2022 21:59:57: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 21:59:57: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 21:59:57: #1 total tags in treatment: 9737703 INFO @ Sat, 15 Jan 2022 21:59:57: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 21:59:57: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 21:59:57: #1 tags after filtering in treatment: 5930768 INFO @ Sat, 15 Jan 2022 21:59:57: #1 Redundant rate of treatment: 0.39 INFO @ Sat, 15 Jan 2022 21:59:57: #1 finished! INFO @ Sat, 15 Jan 2022 21:59:57: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 21:59:57: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 21:59:57: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 21:59:57: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 21:59:57: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8245498/SRX8245498.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245498/SRX8245498.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245498/SRX8245498.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245498/SRX8245498.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 21:59:59: 18000000 INFO @ Sat, 15 Jan 2022 22:00:01: 18000000 INFO @ Sat, 15 Jan 2022 22:00:10: 19000000 INFO @ Sat, 15 Jan 2022 22:00:11: 19000000 INFO @ Sat, 15 Jan 2022 22:00:20: 20000000 INFO @ Sat, 15 Jan 2022 22:00:21: 20000000 INFO @ Sat, 15 Jan 2022 22:00:30: 21000000 INFO @ Sat, 15 Jan 2022 22:00:31: 21000000 INFO @ Sat, 15 Jan 2022 22:00:38: 22000000 INFO @ Sat, 15 Jan 2022 22:00:42: 22000000 INFO @ Sat, 15 Jan 2022 22:00:48: 23000000 INFO @ Sat, 15 Jan 2022 22:00:52: 23000000 INFO @ Sat, 15 Jan 2022 22:00:57: 24000000 INFO @ Sat, 15 Jan 2022 22:01:03: 24000000 INFO @ Sat, 15 Jan 2022 22:01:03: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 22:01:03: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 22:01:03: #1 total tags in treatment: 9737703 INFO @ Sat, 15 Jan 2022 22:01:03: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 22:01:03: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 22:01:04: #1 tags after filtering in treatment: 5930768 INFO @ Sat, 15 Jan 2022 22:01:04: #1 Redundant rate of treatment: 0.39 INFO @ Sat, 15 Jan 2022 22:01:04: #1 finished! INFO @ Sat, 15 Jan 2022 22:01:04: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 22:01:04: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 22:01:04: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 22:01:04: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 22:01:04: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8245498/SRX8245498.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245498/SRX8245498.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245498/SRX8245498.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245498/SRX8245498.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 22:01:11: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 22:01:11: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 22:01:11: #1 total tags in treatment: 9737703 INFO @ Sat, 15 Jan 2022 22:01:11: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 22:01:11: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 22:01:11: #1 tags after filtering in treatment: 5930768 INFO @ Sat, 15 Jan 2022 22:01:11: #1 Redundant rate of treatment: 0.39 INFO @ Sat, 15 Jan 2022 22:01:11: #1 finished! INFO @ Sat, 15 Jan 2022 22:01:11: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 22:01:11: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 22:01:11: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 22:01:11: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 22:01:11: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8245498/SRX8245498.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245498/SRX8245498.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245498/SRX8245498.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245498/SRX8245498.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling