Job ID = 14521209 SRX = SRX8245497 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 19359964 spots for SRR11684708/SRR11684708.sra Written 19359964 spots for SRR11684708/SRR11684708.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:15:10 19359964 reads; of these: 19359964 (100.00%) were paired; of these: 7788437 (40.23%) aligned concordantly 0 times 10276060 (53.08%) aligned concordantly exactly 1 time 1295467 (6.69%) aligned concordantly >1 times ---- 7788437 pairs aligned concordantly 0 times; of these: 300375 (3.86%) aligned discordantly 1 time ---- 7488062 pairs aligned 0 times concordantly or discordantly; of these: 14976124 mates make up the pairs; of these: 10134208 (67.67%) aligned 0 times 4144574 (27.67%) aligned exactly 1 time 697342 (4.66%) aligned >1 times 73.83% overall alignment rate Time searching: 00:15:10 Overall time: 00:15:10 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 12 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 1326189 / 11869984 = 0.1117 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 21:03:50: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8245497/SRX8245497.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8245497/SRX8245497.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8245497/SRX8245497.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8245497/SRX8245497.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 21:03:50: #1 read tag files... INFO @ Sat, 15 Jan 2022 21:03:50: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 21:03:58: 1000000 INFO @ Sat, 15 Jan 2022 21:04:05: 2000000 INFO @ Sat, 15 Jan 2022 21:04:12: 3000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 21:04:20: 4000000 INFO @ Sat, 15 Jan 2022 21:04:20: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8245497/SRX8245497.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8245497/SRX8245497.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8245497/SRX8245497.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8245497/SRX8245497.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 21:04:20: #1 read tag files... INFO @ Sat, 15 Jan 2022 21:04:20: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 21:04:27: 5000000 INFO @ Sat, 15 Jan 2022 21:04:29: 1000000 INFO @ Sat, 15 Jan 2022 21:04:34: 6000000 INFO @ Sat, 15 Jan 2022 21:04:36: 2000000 INFO @ Sat, 15 Jan 2022 21:04:42: 7000000 INFO @ Sat, 15 Jan 2022 21:04:43: 3000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 21:04:49: 8000000 INFO @ Sat, 15 Jan 2022 21:04:50: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8245497/SRX8245497.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8245497/SRX8245497.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8245497/SRX8245497.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8245497/SRX8245497.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 21:04:50: #1 read tag files... INFO @ Sat, 15 Jan 2022 21:04:50: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 21:04:51: 4000000 INFO @ Sat, 15 Jan 2022 21:04:56: 9000000 INFO @ Sat, 15 Jan 2022 21:04:59: 5000000 INFO @ Sat, 15 Jan 2022 21:04:59: 1000000 INFO @ Sat, 15 Jan 2022 21:05:04: 10000000 INFO @ Sat, 15 Jan 2022 21:05:06: 6000000 INFO @ Sat, 15 Jan 2022 21:05:08: 2000000 INFO @ Sat, 15 Jan 2022 21:05:11: 11000000 INFO @ Sat, 15 Jan 2022 21:05:14: 7000000 INFO @ Sat, 15 Jan 2022 21:05:16: 3000000 INFO @ Sat, 15 Jan 2022 21:05:19: 12000000 INFO @ Sat, 15 Jan 2022 21:05:23: 8000000 INFO @ Sat, 15 Jan 2022 21:05:25: 4000000 INFO @ Sat, 15 Jan 2022 21:05:27: 13000000 INFO @ Sat, 15 Jan 2022 21:05:31: 9000000 INFO @ Sat, 15 Jan 2022 21:05:34: 5000000 INFO @ Sat, 15 Jan 2022 21:05:34: 14000000 INFO @ Sat, 15 Jan 2022 21:05:39: 10000000 INFO @ Sat, 15 Jan 2022 21:05:42: 15000000 INFO @ Sat, 15 Jan 2022 21:05:43: 6000000 INFO @ Sat, 15 Jan 2022 21:05:47: 11000000 INFO @ Sat, 15 Jan 2022 21:05:50: 16000000 INFO @ Sat, 15 Jan 2022 21:05:51: 7000000 INFO @ Sat, 15 Jan 2022 21:05:56: 12000000 INFO @ Sat, 15 Jan 2022 21:05:57: 17000000 INFO @ Sat, 15 Jan 2022 21:05:59: 8000000 INFO @ Sat, 15 Jan 2022 21:06:05: 13000000 INFO @ Sat, 15 Jan 2022 21:06:05: 18000000 INFO @ Sat, 15 Jan 2022 21:06:08: 9000000 INFO @ Sat, 15 Jan 2022 21:06:13: 19000000 INFO @ Sat, 15 Jan 2022 21:06:13: 14000000 INFO @ Sat, 15 Jan 2022 21:06:16: 10000000 INFO @ Sat, 15 Jan 2022 21:06:22: 20000000 INFO @ Sat, 15 Jan 2022 21:06:22: 15000000 INFO @ Sat, 15 Jan 2022 21:06:24: 11000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 21:06:30: 21000000 INFO @ Sat, 15 Jan 2022 21:06:30: 16000000 INFO @ Sat, 15 Jan 2022 21:06:33: 12000000 INFO @ Sat, 15 Jan 2022 21:06:38: 22000000 INFO @ Sat, 15 Jan 2022 21:06:39: 17000000 INFO @ Sat, 15 Jan 2022 21:06:41: 13000000 BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 21:06:46: 23000000 INFO @ Sat, 15 Jan 2022 21:06:47: 18000000 INFO @ Sat, 15 Jan 2022 21:06:50: 14000000 INFO @ Sat, 15 Jan 2022 21:06:53: 24000000 INFO @ Sat, 15 Jan 2022 21:06:56: 19000000 INFO @ Sat, 15 Jan 2022 21:06:58: 15000000 INFO @ Sat, 15 Jan 2022 21:07:02: 25000000 INFO @ Sat, 15 Jan 2022 21:07:04: 20000000 INFO @ Sat, 15 Jan 2022 21:07:07: 16000000 INFO @ Sat, 15 Jan 2022 21:07:10: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 21:07:10: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 21:07:10: #1 total tags in treatment: 10261557 INFO @ Sat, 15 Jan 2022 21:07:10: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 21:07:10: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 21:07:10: #1 tags after filtering in treatment: 6650252 INFO @ Sat, 15 Jan 2022 21:07:10: #1 Redundant rate of treatment: 0.35 INFO @ Sat, 15 Jan 2022 21:07:10: #1 finished! INFO @ Sat, 15 Jan 2022 21:07:10: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 21:07:10: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 21:07:11: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 21:07:11: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 21:07:11: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8245497/SRX8245497.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245497/SRX8245497.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245497/SRX8245497.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245497/SRX8245497.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 21:07:13: 21000000 INFO @ Sat, 15 Jan 2022 21:07:15: 17000000 INFO @ Sat, 15 Jan 2022 21:07:20: 22000000 INFO @ Sat, 15 Jan 2022 21:07:24: 18000000 INFO @ Sat, 15 Jan 2022 21:07:28: 23000000 INFO @ Sat, 15 Jan 2022 21:07:32: 19000000 INFO @ Sat, 15 Jan 2022 21:07:35: 24000000 INFO @ Sat, 15 Jan 2022 21:07:42: 20000000 INFO @ Sat, 15 Jan 2022 21:07:43: 25000000 INFO @ Sat, 15 Jan 2022 21:07:50: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 21:07:50: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 21:07:50: #1 total tags in treatment: 10261557 INFO @ Sat, 15 Jan 2022 21:07:50: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 21:07:50: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 21:07:50: #1 tags after filtering in treatment: 6650252 INFO @ Sat, 15 Jan 2022 21:07:50: #1 Redundant rate of treatment: 0.35 INFO @ Sat, 15 Jan 2022 21:07:50: #1 finished! INFO @ Sat, 15 Jan 2022 21:07:50: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 21:07:50: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 21:07:51: 21000000 INFO @ Sat, 15 Jan 2022 21:07:51: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 21:07:51: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 21:07:51: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8245497/SRX8245497.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245497/SRX8245497.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245497/SRX8245497.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245497/SRX8245497.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 21:07:59: 22000000 INFO @ Sat, 15 Jan 2022 21:08:08: 23000000 INFO @ Sat, 15 Jan 2022 21:08:16: 24000000 INFO @ Sat, 15 Jan 2022 21:08:25: 25000000 INFO @ Sat, 15 Jan 2022 21:08:33: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 21:08:33: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 21:08:33: #1 total tags in treatment: 10261557 INFO @ Sat, 15 Jan 2022 21:08:33: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 21:08:33: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 21:08:33: #1 tags after filtering in treatment: 6650252 INFO @ Sat, 15 Jan 2022 21:08:33: #1 Redundant rate of treatment: 0.35 INFO @ Sat, 15 Jan 2022 21:08:33: #1 finished! INFO @ Sat, 15 Jan 2022 21:08:33: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 21:08:33: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 21:08:34: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 21:08:34: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 21:08:34: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8245497/SRX8245497.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245497/SRX8245497.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245497/SRX8245497.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245497/SRX8245497.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling