Job ID = 14521208 SRX = SRX8245496 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 14561546 spots for SRR11684707/SRR11684707.sra Written 14561546 spots for SRR11684707/SRR11684707.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:17:25 14561546 reads; of these: 14561546 (100.00%) were paired; of these: 2430214 (16.69%) aligned concordantly 0 times 10764305 (73.92%) aligned concordantly exactly 1 time 1367027 (9.39%) aligned concordantly >1 times ---- 2430214 pairs aligned concordantly 0 times; of these: 190476 (7.84%) aligned discordantly 1 time ---- 2239738 pairs aligned 0 times concordantly or discordantly; of these: 4479476 mates make up the pairs; of these: 2564471 (57.25%) aligned 0 times 1640171 (36.62%) aligned exactly 1 time 274834 (6.14%) aligned >1 times 91.19% overall alignment rate Time searching: 00:17:25 Overall time: 00:17:25 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 12 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 1127670 / 12320844 = 0.0915 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 21:05:37: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8245496/SRX8245496.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8245496/SRX8245496.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8245496/SRX8245496.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8245496/SRX8245496.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 21:05:37: #1 read tag files... INFO @ Sat, 15 Jan 2022 21:05:37: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 21:05:43: 1000000 INFO @ Sat, 15 Jan 2022 21:05:49: 2000000 INFO @ Sat, 15 Jan 2022 21:05:55: 3000000 INFO @ Sat, 15 Jan 2022 21:06:01: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 21:06:06: 5000000 INFO @ Sat, 15 Jan 2022 21:06:07: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8245496/SRX8245496.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8245496/SRX8245496.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8245496/SRX8245496.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8245496/SRX8245496.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 21:06:07: #1 read tag files... INFO @ Sat, 15 Jan 2022 21:06:07: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 21:06:13: 6000000 INFO @ Sat, 15 Jan 2022 21:06:13: 1000000 INFO @ Sat, 15 Jan 2022 21:06:20: 7000000 INFO @ Sat, 15 Jan 2022 21:06:20: 2000000 INFO @ Sat, 15 Jan 2022 21:06:26: 8000000 INFO @ Sat, 15 Jan 2022 21:06:27: 3000000 INFO @ Sat, 15 Jan 2022 21:06:33: 9000000 INFO @ Sat, 15 Jan 2022 21:06:34: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 21:06:37: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8245496/SRX8245496.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8245496/SRX8245496.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8245496/SRX8245496.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8245496/SRX8245496.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 21:06:37: #1 read tag files... INFO @ Sat, 15 Jan 2022 21:06:37: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 21:06:40: 10000000 INFO @ Sat, 15 Jan 2022 21:06:41: 5000000 INFO @ Sat, 15 Jan 2022 21:06:42: 1000000 INFO @ Sat, 15 Jan 2022 21:06:47: 11000000 INFO @ Sat, 15 Jan 2022 21:06:48: 6000000 INFO @ Sat, 15 Jan 2022 21:06:48: 2000000 INFO @ Sat, 15 Jan 2022 21:06:54: 3000000 INFO @ Sat, 15 Jan 2022 21:06:54: 12000000 INFO @ Sat, 15 Jan 2022 21:06:55: 7000000 INFO @ Sat, 15 Jan 2022 21:06:59: 4000000 INFO @ Sat, 15 Jan 2022 21:07:01: 13000000 INFO @ Sat, 15 Jan 2022 21:07:02: 8000000 INFO @ Sat, 15 Jan 2022 21:07:05: 5000000 INFO @ Sat, 15 Jan 2022 21:07:08: 14000000 INFO @ Sat, 15 Jan 2022 21:07:08: 9000000 INFO @ Sat, 15 Jan 2022 21:07:10: 6000000 INFO @ Sat, 15 Jan 2022 21:07:15: 10000000 INFO @ Sat, 15 Jan 2022 21:07:16: 15000000 INFO @ Sat, 15 Jan 2022 21:07:16: 7000000 INFO @ Sat, 15 Jan 2022 21:07:21: 11000000 INFO @ Sat, 15 Jan 2022 21:07:23: 8000000 INFO @ Sat, 15 Jan 2022 21:07:23: 16000000 INFO @ Sat, 15 Jan 2022 21:07:28: 12000000 INFO @ Sat, 15 Jan 2022 21:07:29: 9000000 INFO @ Sat, 15 Jan 2022 21:07:30: 17000000 INFO @ Sat, 15 Jan 2022 21:07:35: 10000000 INFO @ Sat, 15 Jan 2022 21:07:35: 13000000 INFO @ Sat, 15 Jan 2022 21:07:37: 18000000 INFO @ Sat, 15 Jan 2022 21:07:41: 11000000 INFO @ Sat, 15 Jan 2022 21:07:43: 14000000 INFO @ Sat, 15 Jan 2022 21:07:44: 19000000 INFO @ Sat, 15 Jan 2022 21:07:46: 12000000 INFO @ Sat, 15 Jan 2022 21:07:50: 15000000 INFO @ Sat, 15 Jan 2022 21:07:52: 20000000 INFO @ Sat, 15 Jan 2022 21:07:52: 13000000 INFO @ Sat, 15 Jan 2022 21:07:58: 16000000 INFO @ Sat, 15 Jan 2022 21:07:58: 14000000 INFO @ Sat, 15 Jan 2022 21:07:59: 21000000 INFO @ Sat, 15 Jan 2022 21:08:04: 15000000 INFO @ Sat, 15 Jan 2022 21:08:06: 17000000 INFO @ Sat, 15 Jan 2022 21:08:07: 22000000 INFO @ Sat, 15 Jan 2022 21:08:10: 16000000 INFO @ Sat, 15 Jan 2022 21:08:13: 18000000 INFO @ Sat, 15 Jan 2022 21:08:14: 23000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 21:08:16: 17000000 INFO @ Sat, 15 Jan 2022 21:08:20: 19000000 INFO @ Sat, 15 Jan 2022 21:08:22: 24000000 INFO @ Sat, 15 Jan 2022 21:08:22: 18000000 INFO @ Sat, 15 Jan 2022 21:08:24: #1 tag size is determined as 75 bps INFO @ Sat, 15 Jan 2022 21:08:24: #1 tag size = 75 INFO @ Sat, 15 Jan 2022 21:08:24: #1 total tags in treatment: 11009571 INFO @ Sat, 15 Jan 2022 21:08:24: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 21:08:24: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 21:08:25: #1 tags after filtering in treatment: 5536836 INFO @ Sat, 15 Jan 2022 21:08:25: #1 Redundant rate of treatment: 0.50 INFO @ Sat, 15 Jan 2022 21:08:25: #1 finished! INFO @ Sat, 15 Jan 2022 21:08:25: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 21:08:25: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 21:08:25: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 21:08:25: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 21:08:25: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8245496/SRX8245496.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245496/SRX8245496.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245496/SRX8245496.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245496/SRX8245496.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 21:08:27: 20000000 INFO @ Sat, 15 Jan 2022 21:08:29: 19000000 BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 21:08:33: 21000000 INFO @ Sat, 15 Jan 2022 21:08:36: 20000000 INFO @ Sat, 15 Jan 2022 21:08:40: 22000000 INFO @ Sat, 15 Jan 2022 21:08:42: 21000000 INFO @ Sat, 15 Jan 2022 21:08:46: 23000000 INFO @ Sat, 15 Jan 2022 21:08:48: 22000000 INFO @ Sat, 15 Jan 2022 21:08:52: 24000000 INFO @ Sat, 15 Jan 2022 21:08:54: #1 tag size is determined as 75 bps INFO @ Sat, 15 Jan 2022 21:08:54: #1 tag size = 75 INFO @ Sat, 15 Jan 2022 21:08:54: #1 total tags in treatment: 11009571 INFO @ Sat, 15 Jan 2022 21:08:54: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 21:08:54: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 21:08:55: #1 tags after filtering in treatment: 5536836 INFO @ Sat, 15 Jan 2022 21:08:55: #1 Redundant rate of treatment: 0.50 INFO @ Sat, 15 Jan 2022 21:08:55: #1 finished! INFO @ Sat, 15 Jan 2022 21:08:55: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 21:08:55: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 21:08:55: 23000000 INFO @ Sat, 15 Jan 2022 21:08:55: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 21:08:55: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 21:08:55: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8245496/SRX8245496.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245496/SRX8245496.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245496/SRX8245496.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245496/SRX8245496.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 21:09:01: 24000000 INFO @ Sat, 15 Jan 2022 21:09:03: #1 tag size is determined as 75 bps INFO @ Sat, 15 Jan 2022 21:09:03: #1 tag size = 75 INFO @ Sat, 15 Jan 2022 21:09:03: #1 total tags in treatment: 11009571 INFO @ Sat, 15 Jan 2022 21:09:03: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 21:09:03: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 21:09:03: #1 tags after filtering in treatment: 5536836 INFO @ Sat, 15 Jan 2022 21:09:03: #1 Redundant rate of treatment: 0.50 INFO @ Sat, 15 Jan 2022 21:09:03: #1 finished! INFO @ Sat, 15 Jan 2022 21:09:03: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 21:09:03: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 21:09:04: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 21:09:04: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 21:09:04: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8245496/SRX8245496.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245496/SRX8245496.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245496/SRX8245496.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245496/SRX8245496.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling