Job ID = 14521207 SRX = SRX8245495 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 12991487 spots for SRR11684706/SRR11684706.sra Written 12991487 spots for SRR11684706/SRR11684706.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:09:34 12991487 reads; of these: 12991487 (100.00%) were paired; of these: 2344902 (18.05%) aligned concordantly 0 times 9874141 (76.00%) aligned concordantly exactly 1 time 772444 (5.95%) aligned concordantly >1 times ---- 2344902 pairs aligned concordantly 0 times; of these: 244337 (10.42%) aligned discordantly 1 time ---- 2100565 pairs aligned 0 times concordantly or discordantly; of these: 4201130 mates make up the pairs; of these: 2434700 (57.95%) aligned 0 times 1570201 (37.38%) aligned exactly 1 time 196229 (4.67%) aligned >1 times 90.63% overall alignment rate Time searching: 00:09:34 Overall time: 00:09:34 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 12 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 1028474 / 10889996 = 0.0944 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 20:53:57: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8245495/SRX8245495.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8245495/SRX8245495.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8245495/SRX8245495.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8245495/SRX8245495.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 20:53:57: #1 read tag files... INFO @ Sat, 15 Jan 2022 20:53:57: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 20:54:04: 1000000 INFO @ Sat, 15 Jan 2022 20:54:10: 2000000 INFO @ Sat, 15 Jan 2022 20:54:17: 3000000 INFO @ Sat, 15 Jan 2022 20:54:24: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 20:54:27: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8245495/SRX8245495.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8245495/SRX8245495.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8245495/SRX8245495.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8245495/SRX8245495.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 20:54:27: #1 read tag files... INFO @ Sat, 15 Jan 2022 20:54:27: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 20:54:32: 5000000 INFO @ Sat, 15 Jan 2022 20:54:35: 1000000 INFO @ Sat, 15 Jan 2022 20:54:39: 6000000 INFO @ Sat, 15 Jan 2022 20:54:43: 2000000 INFO @ Sat, 15 Jan 2022 20:54:47: 7000000 INFO @ Sat, 15 Jan 2022 20:54:50: 3000000 INFO @ Sat, 15 Jan 2022 20:54:54: 8000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 20:54:57: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8245495/SRX8245495.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8245495/SRX8245495.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8245495/SRX8245495.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8245495/SRX8245495.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 20:54:57: #1 read tag files... INFO @ Sat, 15 Jan 2022 20:54:57: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 20:54:58: 4000000 INFO @ Sat, 15 Jan 2022 20:55:02: 9000000 INFO @ Sat, 15 Jan 2022 20:55:04: 1000000 INFO @ Sat, 15 Jan 2022 20:55:06: 5000000 INFO @ Sat, 15 Jan 2022 20:55:09: 10000000 INFO @ Sat, 15 Jan 2022 20:55:11: 2000000 INFO @ Sat, 15 Jan 2022 20:55:13: 6000000 INFO @ Sat, 15 Jan 2022 20:55:17: 11000000 INFO @ Sat, 15 Jan 2022 20:55:18: 3000000 INFO @ Sat, 15 Jan 2022 20:55:21: 7000000 INFO @ Sat, 15 Jan 2022 20:55:24: 12000000 INFO @ Sat, 15 Jan 2022 20:55:25: 4000000 INFO @ Sat, 15 Jan 2022 20:55:29: 8000000 INFO @ Sat, 15 Jan 2022 20:55:32: 5000000 INFO @ Sat, 15 Jan 2022 20:55:32: 13000000 INFO @ Sat, 15 Jan 2022 20:55:37: 9000000 INFO @ Sat, 15 Jan 2022 20:55:39: 6000000 INFO @ Sat, 15 Jan 2022 20:55:40: 14000000 INFO @ Sat, 15 Jan 2022 20:55:45: 10000000 INFO @ Sat, 15 Jan 2022 20:55:45: 7000000 INFO @ Sat, 15 Jan 2022 20:55:48: 15000000 INFO @ Sat, 15 Jan 2022 20:55:52: 8000000 INFO @ Sat, 15 Jan 2022 20:55:52: 11000000 INFO @ Sat, 15 Jan 2022 20:55:55: 16000000 INFO @ Sat, 15 Jan 2022 20:55:59: 9000000 INFO @ Sat, 15 Jan 2022 20:56:00: 12000000 INFO @ Sat, 15 Jan 2022 20:56:03: 17000000 INFO @ Sat, 15 Jan 2022 20:56:05: 10000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 20:56:08: 13000000 INFO @ Sat, 15 Jan 2022 20:56:11: 18000000 INFO @ Sat, 15 Jan 2022 20:56:12: 11000000 INFO @ Sat, 15 Jan 2022 20:56:16: 14000000 BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 20:56:18: 19000000 INFO @ Sat, 15 Jan 2022 20:56:19: 12000000 INFO @ Sat, 15 Jan 2022 20:56:24: 15000000 INFO @ Sat, 15 Jan 2022 20:56:26: 13000000 INFO @ Sat, 15 Jan 2022 20:56:26: 20000000 INFO @ Sat, 15 Jan 2022 20:56:32: 16000000 INFO @ Sat, 15 Jan 2022 20:56:33: 14000000 INFO @ Sat, 15 Jan 2022 20:56:34: 21000000 INFO @ Sat, 15 Jan 2022 20:56:38: #1 tag size is determined as 75 bps INFO @ Sat, 15 Jan 2022 20:56:38: #1 tag size = 75 INFO @ Sat, 15 Jan 2022 20:56:38: #1 total tags in treatment: 9626320 INFO @ Sat, 15 Jan 2022 20:56:38: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 20:56:38: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 20:56:38: #1 tags after filtering in treatment: 4951276 INFO @ Sat, 15 Jan 2022 20:56:38: #1 Redundant rate of treatment: 0.49 INFO @ Sat, 15 Jan 2022 20:56:38: #1 finished! INFO @ Sat, 15 Jan 2022 20:56:38: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 20:56:38: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 20:56:38: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 20:56:38: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 20:56:38: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8245495/SRX8245495.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245495/SRX8245495.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245495/SRX8245495.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245495/SRX8245495.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 20:56:39: 15000000 INFO @ Sat, 15 Jan 2022 20:56:40: 17000000 INFO @ Sat, 15 Jan 2022 20:56:46: 16000000 INFO @ Sat, 15 Jan 2022 20:56:48: 18000000 INFO @ Sat, 15 Jan 2022 20:56:53: 17000000 INFO @ Sat, 15 Jan 2022 20:56:56: 19000000 INFO @ Sat, 15 Jan 2022 20:57:00: 18000000 INFO @ Sat, 15 Jan 2022 20:57:04: 20000000 INFO @ Sat, 15 Jan 2022 20:57:07: 19000000 INFO @ Sat, 15 Jan 2022 20:57:12: 21000000 INFO @ Sat, 15 Jan 2022 20:57:13: 20000000 INFO @ Sat, 15 Jan 2022 20:57:15: #1 tag size is determined as 75 bps INFO @ Sat, 15 Jan 2022 20:57:15: #1 tag size = 75 INFO @ Sat, 15 Jan 2022 20:57:15: #1 total tags in treatment: 9626320 INFO @ Sat, 15 Jan 2022 20:57:15: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 20:57:15: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 20:57:16: #1 tags after filtering in treatment: 4951276 INFO @ Sat, 15 Jan 2022 20:57:16: #1 Redundant rate of treatment: 0.49 INFO @ Sat, 15 Jan 2022 20:57:16: #1 finished! INFO @ Sat, 15 Jan 2022 20:57:16: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 20:57:16: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 20:57:16: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 20:57:16: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 20:57:16: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8245495/SRX8245495.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245495/SRX8245495.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245495/SRX8245495.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245495/SRX8245495.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 20:57:20: 21000000 INFO @ Sat, 15 Jan 2022 20:57:23: #1 tag size is determined as 75 bps INFO @ Sat, 15 Jan 2022 20:57:23: #1 tag size = 75 INFO @ Sat, 15 Jan 2022 20:57:23: #1 total tags in treatment: 9626320 INFO @ Sat, 15 Jan 2022 20:57:23: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 20:57:23: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 20:57:23: #1 tags after filtering in treatment: 4951276 INFO @ Sat, 15 Jan 2022 20:57:23: #1 Redundant rate of treatment: 0.49 INFO @ Sat, 15 Jan 2022 20:57:23: #1 finished! INFO @ Sat, 15 Jan 2022 20:57:23: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 20:57:23: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 20:57:23: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 20:57:23: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 20:57:23: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8245495/SRX8245495.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245495/SRX8245495.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245495/SRX8245495.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245495/SRX8245495.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling