Job ID = 14521204 SRX = SRX8245492 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 10961555 spots for SRR11684703/SRR11684703.sra Written 10961555 spots for SRR11684703/SRR11684703.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:06:07 10961555 reads; of these: 10961555 (100.00%) were paired; of these: 5132959 (46.83%) aligned concordantly 0 times 5186031 (47.31%) aligned concordantly exactly 1 time 642565 (5.86%) aligned concordantly >1 times ---- 5132959 pairs aligned concordantly 0 times; of these: 181208 (3.53%) aligned discordantly 1 time ---- 4951751 pairs aligned 0 times concordantly or discordantly; of these: 9903502 mates make up the pairs; of these: 5255396 (53.07%) aligned 0 times 4069320 (41.09%) aligned exactly 1 time 578786 (5.84%) aligned >1 times 76.03% overall alignment rate Time searching: 00:06:07 Overall time: 00:06:07 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 8 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 51501 / 6008988 = 0.0086 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 20:48:38: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8245492/SRX8245492.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8245492/SRX8245492.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8245492/SRX8245492.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8245492/SRX8245492.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 20:48:38: #1 read tag files... INFO @ Sat, 15 Jan 2022 20:48:38: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 20:48:44: 1000000 INFO @ Sat, 15 Jan 2022 20:48:50: 2000000 INFO @ Sat, 15 Jan 2022 20:48:56: 3000000 INFO @ Sat, 15 Jan 2022 20:49:01: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 20:49:07: 5000000 INFO @ Sat, 15 Jan 2022 20:49:08: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8245492/SRX8245492.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8245492/SRX8245492.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8245492/SRX8245492.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8245492/SRX8245492.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 20:49:08: #1 read tag files... INFO @ Sat, 15 Jan 2022 20:49:08: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 20:49:14: 6000000 INFO @ Sat, 15 Jan 2022 20:49:15: 1000000 INFO @ Sat, 15 Jan 2022 20:49:20: 7000000 INFO @ Sat, 15 Jan 2022 20:49:21: 2000000 INFO @ Sat, 15 Jan 2022 20:49:26: 8000000 INFO @ Sat, 15 Jan 2022 20:49:27: 3000000 INFO @ Sat, 15 Jan 2022 20:49:33: 9000000 INFO @ Sat, 15 Jan 2022 20:49:33: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 20:49:38: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8245492/SRX8245492.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8245492/SRX8245492.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8245492/SRX8245492.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8245492/SRX8245492.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 20:49:38: #1 read tag files... INFO @ Sat, 15 Jan 2022 20:49:38: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 20:49:39: 5000000 INFO @ Sat, 15 Jan 2022 20:49:39: 10000000 INFO @ Sat, 15 Jan 2022 20:49:45: 1000000 INFO @ Sat, 15 Jan 2022 20:49:45: 6000000 INFO @ Sat, 15 Jan 2022 20:49:45: 11000000 INFO @ Sat, 15 Jan 2022 20:49:51: 7000000 INFO @ Sat, 15 Jan 2022 20:49:51: 2000000 INFO @ Sat, 15 Jan 2022 20:49:51: 12000000 INFO @ Sat, 15 Jan 2022 20:49:57: 8000000 INFO @ Sat, 15 Jan 2022 20:49:57: 3000000 INFO @ Sat, 15 Jan 2022 20:49:58: 13000000 INFO @ Sat, 15 Jan 2022 20:50:03: 9000000 INFO @ Sat, 15 Jan 2022 20:50:03: 4000000 INFO @ Sat, 15 Jan 2022 20:50:04: 14000000 INFO @ Sat, 15 Jan 2022 20:50:08: 10000000 INFO @ Sat, 15 Jan 2022 20:50:09: 5000000 INFO @ Sat, 15 Jan 2022 20:50:11: 15000000 INFO @ Sat, 15 Jan 2022 20:50:14: 11000000 INFO @ Sat, 15 Jan 2022 20:50:15: 6000000 INFO @ Sat, 15 Jan 2022 20:50:18: 16000000 INFO @ Sat, 15 Jan 2022 20:50:20: 12000000 INFO @ Sat, 15 Jan 2022 20:50:21: 7000000 INFO @ Sat, 15 Jan 2022 20:50:22: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 20:50:22: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 20:50:22: #1 total tags in treatment: 5777429 INFO @ Sat, 15 Jan 2022 20:50:22: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 20:50:22: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 20:50:22: #1 tags after filtering in treatment: 4781006 INFO @ Sat, 15 Jan 2022 20:50:22: #1 Redundant rate of treatment: 0.17 INFO @ Sat, 15 Jan 2022 20:50:22: #1 finished! INFO @ Sat, 15 Jan 2022 20:50:22: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 20:50:22: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 20:50:22: #2 number of paired peaks: 4 WARNING @ Sat, 15 Jan 2022 20:50:22: Too few paired peaks (4) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 20:50:22: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8245492/SRX8245492.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245492/SRX8245492.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245492/SRX8245492.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245492/SRX8245492.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 20:50:26: 13000000 INFO @ Sat, 15 Jan 2022 20:50:27: 8000000 INFO @ Sat, 15 Jan 2022 20:50:32: 14000000 INFO @ Sat, 15 Jan 2022 20:50:32: 9000000 INFO @ Sat, 15 Jan 2022 20:50:38: 15000000 INFO @ Sat, 15 Jan 2022 20:50:38: 10000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 20:50:44: 16000000 INFO @ Sat, 15 Jan 2022 20:50:44: 11000000 INFO @ Sat, 15 Jan 2022 20:50:47: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 20:50:47: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 20:50:47: #1 total tags in treatment: 5777429 INFO @ Sat, 15 Jan 2022 20:50:47: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 20:50:47: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 20:50:47: #1 tags after filtering in treatment: 4781006 INFO @ Sat, 15 Jan 2022 20:50:47: #1 Redundant rate of treatment: 0.17 INFO @ Sat, 15 Jan 2022 20:50:47: #1 finished! INFO @ Sat, 15 Jan 2022 20:50:47: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 20:50:47: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 20:50:47: #2 number of paired peaks: 4 WARNING @ Sat, 15 Jan 2022 20:50:47: Too few paired peaks (4) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 20:50:47: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8245492/SRX8245492.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245492/SRX8245492.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245492/SRX8245492.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245492/SRX8245492.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 20:50:50: 12000000 BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 20:50:55: 13000000 INFO @ Sat, 15 Jan 2022 20:51:00: 14000000 INFO @ Sat, 15 Jan 2022 20:51:06: 15000000 INFO @ Sat, 15 Jan 2022 20:51:11: 16000000 INFO @ Sat, 15 Jan 2022 20:51:13: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 20:51:13: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 20:51:13: #1 total tags in treatment: 5777429 INFO @ Sat, 15 Jan 2022 20:51:13: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 20:51:13: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 20:51:13: #1 tags after filtering in treatment: 4781006 INFO @ Sat, 15 Jan 2022 20:51:13: #1 Redundant rate of treatment: 0.17 INFO @ Sat, 15 Jan 2022 20:51:13: #1 finished! INFO @ Sat, 15 Jan 2022 20:51:13: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 20:51:13: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 20:51:14: #2 number of paired peaks: 4 WARNING @ Sat, 15 Jan 2022 20:51:14: Too few paired peaks (4) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 20:51:14: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8245492/SRX8245492.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245492/SRX8245492.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245492/SRX8245492.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245492/SRX8245492.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling