Job ID = 14521152 SRX = SRX8245484 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 12552023 spots for SRR11684695/SRR11684695.sra Written 12552023 spots for SRR11684695/SRR11684695.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:06:52 12552023 reads; of these: 12552023 (100.00%) were paired; of these: 5667200 (45.15%) aligned concordantly 0 times 6251937 (49.81%) aligned concordantly exactly 1 time 632886 (5.04%) aligned concordantly >1 times ---- 5667200 pairs aligned concordantly 0 times; of these: 210946 (3.72%) aligned discordantly 1 time ---- 5456254 pairs aligned 0 times concordantly or discordantly; of these: 10912508 mates make up the pairs; of these: 6196172 (56.78%) aligned 0 times 4190408 (38.40%) aligned exactly 1 time 525928 (4.82%) aligned >1 times 75.32% overall alignment rate Time searching: 00:06:52 Overall time: 00:06:52 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 8 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 61683 / 7094374 = 0.0087 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 20:45:25: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8245484/SRX8245484.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8245484/SRX8245484.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8245484/SRX8245484.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8245484/SRX8245484.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 20:45:25: #1 read tag files... INFO @ Sat, 15 Jan 2022 20:45:25: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 20:45:31: 1000000 INFO @ Sat, 15 Jan 2022 20:45:38: 2000000 INFO @ Sat, 15 Jan 2022 20:45:44: 3000000 INFO @ Sat, 15 Jan 2022 20:45:51: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 20:45:55: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8245484/SRX8245484.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8245484/SRX8245484.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8245484/SRX8245484.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8245484/SRX8245484.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 20:45:55: #1 read tag files... INFO @ Sat, 15 Jan 2022 20:45:55: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 20:45:58: 5000000 INFO @ Sat, 15 Jan 2022 20:46:02: 1000000 INFO @ Sat, 15 Jan 2022 20:46:05: 6000000 INFO @ Sat, 15 Jan 2022 20:46:10: 2000000 INFO @ Sat, 15 Jan 2022 20:46:12: 7000000 INFO @ Sat, 15 Jan 2022 20:46:17: 3000000 INFO @ Sat, 15 Jan 2022 20:46:19: 8000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 20:46:25: 4000000 INFO @ Sat, 15 Jan 2022 20:46:25: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8245484/SRX8245484.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8245484/SRX8245484.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8245484/SRX8245484.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8245484/SRX8245484.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 20:46:25: #1 read tag files... INFO @ Sat, 15 Jan 2022 20:46:25: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 20:46:26: 9000000 INFO @ Sat, 15 Jan 2022 20:46:32: 1000000 INFO @ Sat, 15 Jan 2022 20:46:32: 5000000 INFO @ Sat, 15 Jan 2022 20:46:34: 10000000 INFO @ Sat, 15 Jan 2022 20:46:39: 2000000 INFO @ Sat, 15 Jan 2022 20:46:40: 6000000 INFO @ Sat, 15 Jan 2022 20:46:41: 11000000 INFO @ Sat, 15 Jan 2022 20:46:46: 3000000 INFO @ Sat, 15 Jan 2022 20:46:47: 7000000 INFO @ Sat, 15 Jan 2022 20:46:48: 12000000 INFO @ Sat, 15 Jan 2022 20:46:53: 4000000 INFO @ Sat, 15 Jan 2022 20:46:54: 8000000 INFO @ Sat, 15 Jan 2022 20:46:55: 13000000 INFO @ Sat, 15 Jan 2022 20:47:00: 5000000 INFO @ Sat, 15 Jan 2022 20:47:02: 9000000 INFO @ Sat, 15 Jan 2022 20:47:03: 14000000 INFO @ Sat, 15 Jan 2022 20:47:06: 6000000 INFO @ Sat, 15 Jan 2022 20:47:09: 10000000 INFO @ Sat, 15 Jan 2022 20:47:10: 15000000 INFO @ Sat, 15 Jan 2022 20:47:13: 7000000 INFO @ Sat, 15 Jan 2022 20:47:17: 11000000 INFO @ Sat, 15 Jan 2022 20:47:17: 16000000 INFO @ Sat, 15 Jan 2022 20:47:20: 8000000 INFO @ Sat, 15 Jan 2022 20:47:23: 17000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 20:47:24: 12000000 INFO @ Sat, 15 Jan 2022 20:47:27: 9000000 INFO @ Sat, 15 Jan 2022 20:47:30: 18000000 INFO @ Sat, 15 Jan 2022 20:47:31: 13000000 INFO @ Sat, 15 Jan 2022 20:47:34: 10000000 INFO @ Sat, 15 Jan 2022 20:47:36: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 20:47:36: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 20:47:36: #1 total tags in treatment: 6823536 INFO @ Sat, 15 Jan 2022 20:47:36: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 20:47:36: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 20:47:36: #1 tags after filtering in treatment: 5413641 INFO @ Sat, 15 Jan 2022 20:47:36: #1 Redundant rate of treatment: 0.21 INFO @ Sat, 15 Jan 2022 20:47:36: #1 finished! INFO @ Sat, 15 Jan 2022 20:47:36: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 20:47:36: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 20:47:36: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 20:47:36: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 20:47:36: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8245484/SRX8245484.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245484/SRX8245484.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245484/SRX8245484.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245484/SRX8245484.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 20:47:38: 14000000 INFO @ Sat, 15 Jan 2022 20:47:41: 11000000 INFO @ Sat, 15 Jan 2022 20:47:46: 15000000 INFO @ Sat, 15 Jan 2022 20:47:48: 12000000 INFO @ Sat, 15 Jan 2022 20:47:52: 16000000 INFO @ Sat, 15 Jan 2022 20:47:55: 13000000 INFO @ Sat, 15 Jan 2022 20:47:59: 17000000 INFO @ Sat, 15 Jan 2022 20:48:01: 14000000 INFO @ Sat, 15 Jan 2022 20:48:06: 18000000 INFO @ Sat, 15 Jan 2022 20:48:08: 15000000 INFO @ Sat, 15 Jan 2022 20:48:12: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 20:48:12: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 20:48:12: #1 total tags in treatment: 6823536 INFO @ Sat, 15 Jan 2022 20:48:12: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 20:48:12: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 20:48:12: #1 tags after filtering in treatment: 5413641 INFO @ Sat, 15 Jan 2022 20:48:12: #1 Redundant rate of treatment: 0.21 INFO @ Sat, 15 Jan 2022 20:48:12: #1 finished! INFO @ Sat, 15 Jan 2022 20:48:12: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 20:48:12: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 20:48:12: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 20:48:12: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 20:48:12: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8245484/SRX8245484.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 32 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245484/SRX8245484.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245484/SRX8245484.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245484/SRX8245484.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 20:48:15: 16000000 INFO @ Sat, 15 Jan 2022 20:48:22: 17000000 INFO @ Sat, 15 Jan 2022 20:48:27: 18000000 INFO @ Sat, 15 Jan 2022 20:48:31: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 20:48:31: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 20:48:31: #1 total tags in treatment: 6823536 INFO @ Sat, 15 Jan 2022 20:48:31: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 20:48:31: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 20:48:31: #1 tags after filtering in treatment: 5413641 INFO @ Sat, 15 Jan 2022 20:48:31: #1 Redundant rate of treatment: 0.21 INFO @ Sat, 15 Jan 2022 20:48:31: #1 finished! INFO @ Sat, 15 Jan 2022 20:48:31: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 20:48:31: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 20:48:32: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 20:48:32: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 20:48:32: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8245484/SRX8245484.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245484/SRX8245484.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245484/SRX8245484.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245484/SRX8245484.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling