Job ID = 14521151 SRX = SRX8245483 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 22047275 spots for SRR11684694/SRR11684694.sra Written 22047275 spots for SRR11684694/SRR11684694.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:16:50 22047275 reads; of these: 22047275 (100.00%) were paired; of these: 9811656 (44.50%) aligned concordantly 0 times 11138448 (50.52%) aligned concordantly exactly 1 time 1097171 (4.98%) aligned concordantly >1 times ---- 9811656 pairs aligned concordantly 0 times; of these: 245857 (2.51%) aligned discordantly 1 time ---- 9565799 pairs aligned 0 times concordantly or discordantly; of these: 19131598 mates make up the pairs; of these: 10512675 (54.95%) aligned 0 times 7716386 (40.33%) aligned exactly 1 time 902537 (4.72%) aligned >1 times 76.16% overall alignment rate Time searching: 00:16:50 Overall time: 00:16:50 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 16 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 156488 / 12479497 = 0.0125 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 21:08:45: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8245483/SRX8245483.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8245483/SRX8245483.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8245483/SRX8245483.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8245483/SRX8245483.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 21:08:45: #1 read tag files... INFO @ Sat, 15 Jan 2022 21:08:45: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 21:08:50: 1000000 INFO @ Sat, 15 Jan 2022 21:08:56: 2000000 INFO @ Sat, 15 Jan 2022 21:09:01: 3000000 INFO @ Sat, 15 Jan 2022 21:09:07: 4000000 INFO @ Sat, 15 Jan 2022 21:09:13: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 21:09:14: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8245483/SRX8245483.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8245483/SRX8245483.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8245483/SRX8245483.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8245483/SRX8245483.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 21:09:14: #1 read tag files... INFO @ Sat, 15 Jan 2022 21:09:14: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 21:09:18: 6000000 INFO @ Sat, 15 Jan 2022 21:09:20: 1000000 INFO @ Sat, 15 Jan 2022 21:09:24: 7000000 INFO @ Sat, 15 Jan 2022 21:09:27: 2000000 INFO @ Sat, 15 Jan 2022 21:09:30: 8000000 INFO @ Sat, 15 Jan 2022 21:09:33: 3000000 INFO @ Sat, 15 Jan 2022 21:09:36: 9000000 INFO @ Sat, 15 Jan 2022 21:09:40: 4000000 INFO @ Sat, 15 Jan 2022 21:09:42: 10000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 21:09:45: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8245483/SRX8245483.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8245483/SRX8245483.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8245483/SRX8245483.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8245483/SRX8245483.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 21:09:45: #1 read tag files... INFO @ Sat, 15 Jan 2022 21:09:45: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 21:09:46: 5000000 INFO @ Sat, 15 Jan 2022 21:09:48: 11000000 INFO @ Sat, 15 Jan 2022 21:09:50: 1000000 INFO @ Sat, 15 Jan 2022 21:09:52: 6000000 INFO @ Sat, 15 Jan 2022 21:09:54: 12000000 INFO @ Sat, 15 Jan 2022 21:09:56: 2000000 INFO @ Sat, 15 Jan 2022 21:09:59: 7000000 INFO @ Sat, 15 Jan 2022 21:10:00: 13000000 INFO @ Sat, 15 Jan 2022 21:10:01: 3000000 INFO @ Sat, 15 Jan 2022 21:10:05: 8000000 INFO @ Sat, 15 Jan 2022 21:10:06: 14000000 INFO @ Sat, 15 Jan 2022 21:10:07: 4000000 INFO @ Sat, 15 Jan 2022 21:10:12: 9000000 INFO @ Sat, 15 Jan 2022 21:10:12: 15000000 INFO @ Sat, 15 Jan 2022 21:10:13: 5000000 INFO @ Sat, 15 Jan 2022 21:10:18: 10000000 INFO @ Sat, 15 Jan 2022 21:10:18: 6000000 INFO @ Sat, 15 Jan 2022 21:10:18: 16000000 INFO @ Sat, 15 Jan 2022 21:10:24: 7000000 INFO @ Sat, 15 Jan 2022 21:10:24: 11000000 INFO @ Sat, 15 Jan 2022 21:10:25: 17000000 INFO @ Sat, 15 Jan 2022 21:10:29: 8000000 INFO @ Sat, 15 Jan 2022 21:10:31: 12000000 INFO @ Sat, 15 Jan 2022 21:10:31: 18000000 INFO @ Sat, 15 Jan 2022 21:10:35: 9000000 INFO @ Sat, 15 Jan 2022 21:10:36: 13000000 INFO @ Sat, 15 Jan 2022 21:10:37: 19000000 INFO @ Sat, 15 Jan 2022 21:10:41: 10000000 INFO @ Sat, 15 Jan 2022 21:10:42: 14000000 INFO @ Sat, 15 Jan 2022 21:10:43: 20000000 INFO @ Sat, 15 Jan 2022 21:10:46: 11000000 INFO @ Sat, 15 Jan 2022 21:10:48: 15000000 INFO @ Sat, 15 Jan 2022 21:10:49: 21000000 INFO @ Sat, 15 Jan 2022 21:10:52: 12000000 INFO @ Sat, 15 Jan 2022 21:10:54: 16000000 INFO @ Sat, 15 Jan 2022 21:10:56: 22000000 INFO @ Sat, 15 Jan 2022 21:10:58: 13000000 INFO @ Sat, 15 Jan 2022 21:11:00: 17000000 INFO @ Sat, 15 Jan 2022 21:11:02: 23000000 INFO @ Sat, 15 Jan 2022 21:11:03: 14000000 INFO @ Sat, 15 Jan 2022 21:11:06: 18000000 INFO @ Sat, 15 Jan 2022 21:11:08: 24000000 INFO @ Sat, 15 Jan 2022 21:11:09: 15000000 INFO @ Sat, 15 Jan 2022 21:11:13: 19000000 INFO @ Sat, 15 Jan 2022 21:11:15: 16000000 INFO @ Sat, 15 Jan 2022 21:11:15: 25000000 INFO @ Sat, 15 Jan 2022 21:11:19: 20000000 INFO @ Sat, 15 Jan 2022 21:11:20: 17000000 INFO @ Sat, 15 Jan 2022 21:11:21: 26000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 21:11:26: 18000000 INFO @ Sat, 15 Jan 2022 21:11:26: 21000000 INFO @ Sat, 15 Jan 2022 21:11:27: 27000000 INFO @ Sat, 15 Jan 2022 21:11:31: 19000000 INFO @ Sat, 15 Jan 2022 21:11:32: 22000000 INFO @ Sat, 15 Jan 2022 21:11:34: 28000000 INFO @ Sat, 15 Jan 2022 21:11:37: 20000000 BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 21:11:38: 23000000 INFO @ Sat, 15 Jan 2022 21:11:40: 29000000 INFO @ Sat, 15 Jan 2022 21:11:42: 21000000 INFO @ Sat, 15 Jan 2022 21:11:45: 24000000 INFO @ Sat, 15 Jan 2022 21:11:46: 30000000 INFO @ Sat, 15 Jan 2022 21:11:47: 22000000 INFO @ Sat, 15 Jan 2022 21:11:51: 25000000 INFO @ Sat, 15 Jan 2022 21:11:52: 31000000 INFO @ Sat, 15 Jan 2022 21:11:53: 23000000 INFO @ Sat, 15 Jan 2022 21:11:58: 26000000 INFO @ Sat, 15 Jan 2022 21:11:58: 32000000 INFO @ Sat, 15 Jan 2022 21:11:58: 24000000 INFO @ Sat, 15 Jan 2022 21:12:04: 25000000 INFO @ Sat, 15 Jan 2022 21:12:04: 27000000 INFO @ Sat, 15 Jan 2022 21:12:04: 33000000 INFO @ Sat, 15 Jan 2022 21:12:06: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 21:12:06: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 21:12:06: #1 total tags in treatment: 12079711 INFO @ Sat, 15 Jan 2022 21:12:06: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 21:12:06: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 21:12:06: #1 tags after filtering in treatment: 8477241 INFO @ Sat, 15 Jan 2022 21:12:06: #1 Redundant rate of treatment: 0.30 INFO @ Sat, 15 Jan 2022 21:12:06: #1 finished! INFO @ Sat, 15 Jan 2022 21:12:06: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 21:12:06: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 21:12:07: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 21:12:07: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 21:12:07: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8245483/SRX8245483.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245483/SRX8245483.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245483/SRX8245483.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245483/SRX8245483.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 21:12:09: 26000000 INFO @ Sat, 15 Jan 2022 21:12:10: 28000000 INFO @ Sat, 15 Jan 2022 21:12:15: 27000000 INFO @ Sat, 15 Jan 2022 21:12:17: 29000000 INFO @ Sat, 15 Jan 2022 21:12:20: 28000000 INFO @ Sat, 15 Jan 2022 21:12:23: 30000000 INFO @ Sat, 15 Jan 2022 21:12:26: 29000000 INFO @ Sat, 15 Jan 2022 21:12:29: 31000000 INFO @ Sat, 15 Jan 2022 21:12:31: 30000000 INFO @ Sat, 15 Jan 2022 21:12:35: 32000000 INFO @ Sat, 15 Jan 2022 21:12:37: 31000000 INFO @ Sat, 15 Jan 2022 21:12:41: 33000000 INFO @ Sat, 15 Jan 2022 21:12:42: 32000000 INFO @ Sat, 15 Jan 2022 21:12:43: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 21:12:43: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 21:12:43: #1 total tags in treatment: 12079711 INFO @ Sat, 15 Jan 2022 21:12:43: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 21:12:43: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 21:12:43: #1 tags after filtering in treatment: 8477241 INFO @ Sat, 15 Jan 2022 21:12:43: #1 Redundant rate of treatment: 0.30 INFO @ Sat, 15 Jan 2022 21:12:43: #1 finished! INFO @ Sat, 15 Jan 2022 21:12:43: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 21:12:43: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 21:12:43: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 21:12:43: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 21:12:43: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8245483/SRX8245483.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245483/SRX8245483.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245483/SRX8245483.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245483/SRX8245483.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 21:12:48: 33000000 INFO @ Sat, 15 Jan 2022 21:12:49: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 21:12:49: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 21:12:49: #1 total tags in treatment: 12079711 INFO @ Sat, 15 Jan 2022 21:12:49: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 21:12:49: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 21:12:49: #1 tags after filtering in treatment: 8477241 INFO @ Sat, 15 Jan 2022 21:12:49: #1 Redundant rate of treatment: 0.30 INFO @ Sat, 15 Jan 2022 21:12:49: #1 finished! INFO @ Sat, 15 Jan 2022 21:12:49: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 21:12:49: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 21:12:50: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 21:12:50: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 21:12:50: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8245483/SRX8245483.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245483/SRX8245483.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245483/SRX8245483.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245483/SRX8245483.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling