Job ID = 14521150 SRX = SRX8245482 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 13513736 spots for SRR11684693/SRR11684693.sra Written 13513736 spots for SRR11684693/SRR11684693.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:11:30 13513736 reads; of these: 13513736 (100.00%) were paired; of these: 5923990 (43.84%) aligned concordantly 0 times 6925369 (51.25%) aligned concordantly exactly 1 time 664377 (4.92%) aligned concordantly >1 times ---- 5923990 pairs aligned concordantly 0 times; of these: 211139 (3.56%) aligned discordantly 1 time ---- 5712851 pairs aligned 0 times concordantly or discordantly; of these: 11425702 mates make up the pairs; of these: 6368850 (55.74%) aligned 0 times 4525514 (39.61%) aligned exactly 1 time 531338 (4.65%) aligned >1 times 76.44% overall alignment rate Time searching: 00:11:30 Overall time: 00:11:30 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 12 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 64683 / 7799375 = 0.0083 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 20:56:00: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8245482/SRX8245482.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8245482/SRX8245482.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8245482/SRX8245482.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8245482/SRX8245482.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 20:56:00: #1 read tag files... INFO @ Sat, 15 Jan 2022 20:56:00: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 20:56:10: 1000000 INFO @ Sat, 15 Jan 2022 20:56:19: 2000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 20:56:28: 3000000 INFO @ Sat, 15 Jan 2022 20:56:30: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8245482/SRX8245482.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8245482/SRX8245482.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8245482/SRX8245482.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8245482/SRX8245482.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 20:56:30: #1 read tag files... INFO @ Sat, 15 Jan 2022 20:56:30: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 20:56:37: 4000000 INFO @ Sat, 15 Jan 2022 20:56:41: 1000000 INFO @ Sat, 15 Jan 2022 20:56:46: 5000000 INFO @ Sat, 15 Jan 2022 20:56:52: 2000000 INFO @ Sat, 15 Jan 2022 20:56:56: 6000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 20:57:00: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8245482/SRX8245482.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8245482/SRX8245482.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8245482/SRX8245482.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8245482/SRX8245482.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 20:57:00: #1 read tag files... INFO @ Sat, 15 Jan 2022 20:57:00: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 20:57:03: 3000000 INFO @ Sat, 15 Jan 2022 20:57:06: 7000000 INFO @ Sat, 15 Jan 2022 20:57:13: 1000000 INFO @ Sat, 15 Jan 2022 20:57:13: 4000000 INFO @ Sat, 15 Jan 2022 20:57:15: 8000000 INFO @ Sat, 15 Jan 2022 20:57:23: 5000000 INFO @ Sat, 15 Jan 2022 20:57:24: 2000000 INFO @ Sat, 15 Jan 2022 20:57:25: 9000000 INFO @ Sat, 15 Jan 2022 20:57:35: 6000000 INFO @ Sat, 15 Jan 2022 20:57:36: 10000000 INFO @ Sat, 15 Jan 2022 20:57:37: 3000000 INFO @ Sat, 15 Jan 2022 20:57:46: 11000000 INFO @ Sat, 15 Jan 2022 20:57:47: 7000000 INFO @ Sat, 15 Jan 2022 20:57:50: 4000000 INFO @ Sat, 15 Jan 2022 20:57:56: 12000000 INFO @ Sat, 15 Jan 2022 20:57:59: 8000000 INFO @ Sat, 15 Jan 2022 20:58:02: 5000000 INFO @ Sat, 15 Jan 2022 20:58:06: 13000000 INFO @ Sat, 15 Jan 2022 20:58:12: 9000000 INFO @ Sat, 15 Jan 2022 20:58:15: 6000000 INFO @ Sat, 15 Jan 2022 20:58:16: 14000000 INFO @ Sat, 15 Jan 2022 20:58:26: 10000000 INFO @ Sat, 15 Jan 2022 20:58:26: 15000000 INFO @ Sat, 15 Jan 2022 20:58:28: 7000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 20:58:37: 16000000 INFO @ Sat, 15 Jan 2022 20:58:39: 11000000 INFO @ Sat, 15 Jan 2022 20:58:41: 8000000 INFO @ Sat, 15 Jan 2022 20:58:47: 17000000 BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 20:58:53: 12000000 INFO @ Sat, 15 Jan 2022 20:58:55: 9000000 INFO @ Sat, 15 Jan 2022 20:58:58: 18000000 INFO @ Sat, 15 Jan 2022 20:59:07: 13000000 INFO @ Sat, 15 Jan 2022 20:59:08: 19000000 INFO @ Sat, 15 Jan 2022 20:59:09: 10000000 INFO @ Sat, 15 Jan 2022 20:59:18: 20000000 INFO @ Sat, 15 Jan 2022 20:59:20: 14000000 INFO @ Sat, 15 Jan 2022 20:59:22: 11000000 INFO @ Sat, 15 Jan 2022 20:59:23: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 20:59:23: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 20:59:23: #1 total tags in treatment: 7525505 INFO @ Sat, 15 Jan 2022 20:59:23: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 20:59:23: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 20:59:24: #1 tags after filtering in treatment: 5918338 INFO @ Sat, 15 Jan 2022 20:59:24: #1 Redundant rate of treatment: 0.21 INFO @ Sat, 15 Jan 2022 20:59:24: #1 finished! INFO @ Sat, 15 Jan 2022 20:59:24: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 20:59:24: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 20:59:24: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 20:59:24: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 20:59:24: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8245482/SRX8245482.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245482/SRX8245482.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245482/SRX8245482.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245482/SRX8245482.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 20:59:33: 15000000 INFO @ Sat, 15 Jan 2022 20:59:34: 12000000 INFO @ Sat, 15 Jan 2022 20:59:45: 16000000 INFO @ Sat, 15 Jan 2022 20:59:46: 13000000 INFO @ Sat, 15 Jan 2022 20:59:58: 14000000 INFO @ Sat, 15 Jan 2022 20:59:59: 17000000 INFO @ Sat, 15 Jan 2022 21:00:11: 15000000 INFO @ Sat, 15 Jan 2022 21:00:13: 18000000 INFO @ Sat, 15 Jan 2022 21:00:25: 19000000 INFO @ Sat, 15 Jan 2022 21:00:25: 16000000 INFO @ Sat, 15 Jan 2022 21:00:37: 20000000 INFO @ Sat, 15 Jan 2022 21:00:38: 17000000 INFO @ Sat, 15 Jan 2022 21:00:43: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 21:00:43: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 21:00:43: #1 total tags in treatment: 7525505 INFO @ Sat, 15 Jan 2022 21:00:43: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 21:00:43: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 21:00:44: #1 tags after filtering in treatment: 5918338 INFO @ Sat, 15 Jan 2022 21:00:44: #1 Redundant rate of treatment: 0.21 INFO @ Sat, 15 Jan 2022 21:00:44: #1 finished! INFO @ Sat, 15 Jan 2022 21:00:44: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 21:00:44: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 21:00:44: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 21:00:44: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 21:00:44: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8245482/SRX8245482.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245482/SRX8245482.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245482/SRX8245482.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245482/SRX8245482.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 21:00:52: 18000000 INFO @ Sat, 15 Jan 2022 21:01:04: 19000000 INFO @ Sat, 15 Jan 2022 21:01:15: 20000000 INFO @ Sat, 15 Jan 2022 21:01:21: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 21:01:21: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 21:01:21: #1 total tags in treatment: 7525505 INFO @ Sat, 15 Jan 2022 21:01:21: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 21:01:21: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 21:01:22: #1 tags after filtering in treatment: 5918338 INFO @ Sat, 15 Jan 2022 21:01:22: #1 Redundant rate of treatment: 0.21 INFO @ Sat, 15 Jan 2022 21:01:22: #1 finished! INFO @ Sat, 15 Jan 2022 21:01:22: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 21:01:22: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 21:01:22: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 21:01:22: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 21:01:22: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8245482/SRX8245482.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245482/SRX8245482.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245482/SRX8245482.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245482/SRX8245482.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling