Job ID = 14521149 SRX = SRX8245481 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 15848116 spots for SRR11684692/SRR11684692.sra Written 15848116 spots for SRR11684692/SRR11684692.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:12:57 15848116 reads; of these: 15848116 (100.00%) were paired; of these: 7868299 (49.65%) aligned concordantly 0 times 7359231 (46.44%) aligned concordantly exactly 1 time 620586 (3.92%) aligned concordantly >1 times ---- 7868299 pairs aligned concordantly 0 times; of these: 541589 (6.88%) aligned discordantly 1 time ---- 7326710 pairs aligned 0 times concordantly or discordantly; of these: 14653420 mates make up the pairs; of these: 7962270 (54.34%) aligned 0 times 6004141 (40.97%) aligned exactly 1 time 687009 (4.69%) aligned >1 times 74.88% overall alignment rate Time searching: 00:12:57 Overall time: 00:12:57 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 12 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 161067 / 8519821 = 0.0189 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 20:59:52: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8245481/SRX8245481.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8245481/SRX8245481.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8245481/SRX8245481.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8245481/SRX8245481.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 20:59:52: #1 read tag files... INFO @ Sat, 15 Jan 2022 20:59:52: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 20:59:59: 1000000 INFO @ Sat, 15 Jan 2022 21:00:05: 2000000 INFO @ Sat, 15 Jan 2022 21:00:11: 3000000 INFO @ Sat, 15 Jan 2022 21:00:19: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 21:00:22: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8245481/SRX8245481.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8245481/SRX8245481.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8245481/SRX8245481.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8245481/SRX8245481.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 21:00:22: #1 read tag files... INFO @ Sat, 15 Jan 2022 21:00:22: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 21:00:25: 5000000 INFO @ Sat, 15 Jan 2022 21:00:28: 1000000 INFO @ Sat, 15 Jan 2022 21:00:32: 6000000 INFO @ Sat, 15 Jan 2022 21:00:34: 2000000 INFO @ Sat, 15 Jan 2022 21:00:40: 7000000 INFO @ Sat, 15 Jan 2022 21:00:40: 3000000 INFO @ Sat, 15 Jan 2022 21:00:45: 4000000 INFO @ Sat, 15 Jan 2022 21:00:47: 8000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 21:00:51: 5000000 INFO @ Sat, 15 Jan 2022 21:00:52: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8245481/SRX8245481.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8245481/SRX8245481.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8245481/SRX8245481.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8245481/SRX8245481.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 21:00:52: #1 read tag files... INFO @ Sat, 15 Jan 2022 21:00:52: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 21:00:54: 9000000 INFO @ Sat, 15 Jan 2022 21:00:58: 6000000 INFO @ Sat, 15 Jan 2022 21:00:58: 1000000 INFO @ Sat, 15 Jan 2022 21:01:01: 10000000 INFO @ Sat, 15 Jan 2022 21:01:05: 7000000 INFO @ Sat, 15 Jan 2022 21:01:05: 2000000 INFO @ Sat, 15 Jan 2022 21:01:08: 11000000 INFO @ Sat, 15 Jan 2022 21:01:11: 8000000 INFO @ Sat, 15 Jan 2022 21:01:11: 3000000 INFO @ Sat, 15 Jan 2022 21:01:14: 12000000 INFO @ Sat, 15 Jan 2022 21:01:18: 9000000 INFO @ Sat, 15 Jan 2022 21:01:18: 4000000 INFO @ Sat, 15 Jan 2022 21:01:21: 13000000 INFO @ Sat, 15 Jan 2022 21:01:24: 5000000 INFO @ Sat, 15 Jan 2022 21:01:25: 10000000 INFO @ Sat, 15 Jan 2022 21:01:27: 14000000 INFO @ Sat, 15 Jan 2022 21:01:30: 6000000 INFO @ Sat, 15 Jan 2022 21:01:32: 11000000 INFO @ Sat, 15 Jan 2022 21:01:34: 15000000 INFO @ Sat, 15 Jan 2022 21:01:37: 7000000 INFO @ Sat, 15 Jan 2022 21:01:40: 12000000 INFO @ Sat, 15 Jan 2022 21:01:40: 16000000 INFO @ Sat, 15 Jan 2022 21:01:43: 8000000 INFO @ Sat, 15 Jan 2022 21:01:47: 17000000 INFO @ Sat, 15 Jan 2022 21:01:47: 13000000 INFO @ Sat, 15 Jan 2022 21:01:49: 9000000 INFO @ Sat, 15 Jan 2022 21:01:53: 18000000 INFO @ Sat, 15 Jan 2022 21:01:55: 14000000 INFO @ Sat, 15 Jan 2022 21:01:55: 10000000 INFO @ Sat, 15 Jan 2022 21:02:00: 19000000 INFO @ Sat, 15 Jan 2022 21:02:02: 11000000 INFO @ Sat, 15 Jan 2022 21:02:03: 15000000 INFO @ Sat, 15 Jan 2022 21:02:06: 20000000 INFO @ Sat, 15 Jan 2022 21:02:08: 12000000 INFO @ Sat, 15 Jan 2022 21:02:10: 16000000 INFO @ Sat, 15 Jan 2022 21:02:12: 21000000 INFO @ Sat, 15 Jan 2022 21:02:14: 13000000 INFO @ Sat, 15 Jan 2022 21:02:17: 17000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 21:02:19: 22000000 INFO @ Sat, 15 Jan 2022 21:02:21: 14000000 INFO @ Sat, 15 Jan 2022 21:02:24: 18000000 INFO @ Sat, 15 Jan 2022 21:02:25: 23000000 INFO @ Sat, 15 Jan 2022 21:02:27: 15000000 INFO @ Sat, 15 Jan 2022 21:02:28: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 21:02:28: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 21:02:28: #1 total tags in treatment: 7823521 INFO @ Sat, 15 Jan 2022 21:02:28: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 21:02:28: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 21:02:28: #1 tags after filtering in treatment: 6227423 INFO @ Sat, 15 Jan 2022 21:02:28: #1 Redundant rate of treatment: 0.20 INFO @ Sat, 15 Jan 2022 21:02:28: #1 finished! INFO @ Sat, 15 Jan 2022 21:02:28: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 21:02:28: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 21:02:29: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 21:02:29: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 21:02:29: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8245481/SRX8245481.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245481/SRX8245481.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245481/SRX8245481.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245481/SRX8245481.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 21:02:30: 19000000 INFO @ Sat, 15 Jan 2022 21:02:33: 16000000 INFO @ Sat, 15 Jan 2022 21:02:36: 20000000 INFO @ Sat, 15 Jan 2022 21:02:41: 17000000 BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 21:02:43: 21000000 INFO @ Sat, 15 Jan 2022 21:02:47: 18000000 INFO @ Sat, 15 Jan 2022 21:02:51: 22000000 INFO @ Sat, 15 Jan 2022 21:02:54: 19000000 INFO @ Sat, 15 Jan 2022 21:02:58: 23000000 INFO @ Sat, 15 Jan 2022 21:03:00: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 21:03:00: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 21:03:00: #1 total tags in treatment: 7823521 INFO @ Sat, 15 Jan 2022 21:03:00: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 21:03:00: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 21:03:01: #1 tags after filtering in treatment: 6227423 INFO @ Sat, 15 Jan 2022 21:03:01: #1 Redundant rate of treatment: 0.20 INFO @ Sat, 15 Jan 2022 21:03:01: #1 finished! INFO @ Sat, 15 Jan 2022 21:03:01: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 21:03:01: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 21:03:01: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 21:03:01: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 21:03:01: Process for pairing-model is terminated! INFO @ Sat, 15 Jan 2022 21:03:01: 20000000 cut: /home/okishinya/chipatlas/results/sacCer3/SRX8245481/SRX8245481.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245481/SRX8245481.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245481/SRX8245481.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245481/SRX8245481.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 21:03:07: 21000000 INFO @ Sat, 15 Jan 2022 21:03:12: 22000000 INFO @ Sat, 15 Jan 2022 21:03:18: 23000000 INFO @ Sat, 15 Jan 2022 21:03:21: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 21:03:21: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 21:03:21: #1 total tags in treatment: 7823521 INFO @ Sat, 15 Jan 2022 21:03:21: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 21:03:21: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 21:03:21: #1 tags after filtering in treatment: 6227423 INFO @ Sat, 15 Jan 2022 21:03:21: #1 Redundant rate of treatment: 0.20 INFO @ Sat, 15 Jan 2022 21:03:21: #1 finished! INFO @ Sat, 15 Jan 2022 21:03:21: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 21:03:21: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 21:03:21: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 21:03:21: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 21:03:21: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8245481/SRX8245481.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245481/SRX8245481.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245481/SRX8245481.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245481/SRX8245481.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling