Job ID = 14521148 SRX = SRX8245480 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 15380342 spots for SRR11684691/SRR11684691.sra Written 15380342 spots for SRR11684691/SRR11684691.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:11:47 15380342 reads; of these: 15380342 (100.00%) were paired; of these: 7978458 (51.87%) aligned concordantly 0 times 6775537 (44.05%) aligned concordantly exactly 1 time 626347 (4.07%) aligned concordantly >1 times ---- 7978458 pairs aligned concordantly 0 times; of these: 479639 (6.01%) aligned discordantly 1 time ---- 7498819 pairs aligned 0 times concordantly or discordantly; of these: 14997638 mates make up the pairs; of these: 8216731 (54.79%) aligned 0 times 6046086 (40.31%) aligned exactly 1 time 734821 (4.90%) aligned >1 times 73.29% overall alignment rate Time searching: 00:11:47 Overall time: 00:11:47 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 12 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 189585 / 7879873 = 0.0241 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 20:53:59: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8245480/SRX8245480.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8245480/SRX8245480.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8245480/SRX8245480.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8245480/SRX8245480.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 20:53:59: #1 read tag files... INFO @ Sat, 15 Jan 2022 20:53:59: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 20:54:04: 1000000 INFO @ Sat, 15 Jan 2022 20:54:10: 2000000 INFO @ Sat, 15 Jan 2022 20:54:15: 3000000 INFO @ Sat, 15 Jan 2022 20:54:20: 4000000 INFO @ Sat, 15 Jan 2022 20:54:25: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 20:54:29: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8245480/SRX8245480.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8245480/SRX8245480.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8245480/SRX8245480.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8245480/SRX8245480.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 20:54:29: #1 read tag files... INFO @ Sat, 15 Jan 2022 20:54:29: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 20:54:30: 6000000 INFO @ Sat, 15 Jan 2022 20:54:35: 7000000 INFO @ Sat, 15 Jan 2022 20:54:36: 1000000 INFO @ Sat, 15 Jan 2022 20:54:40: 8000000 INFO @ Sat, 15 Jan 2022 20:54:43: 2000000 INFO @ Sat, 15 Jan 2022 20:54:46: 9000000 INFO @ Sat, 15 Jan 2022 20:54:49: 3000000 INFO @ Sat, 15 Jan 2022 20:54:51: 10000000 INFO @ Sat, 15 Jan 2022 20:54:55: 4000000 INFO @ Sat, 15 Jan 2022 20:54:56: 11000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 20:54:59: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8245480/SRX8245480.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8245480/SRX8245480.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8245480/SRX8245480.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8245480/SRX8245480.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 20:54:59: #1 read tag files... INFO @ Sat, 15 Jan 2022 20:54:59: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 20:55:01: 5000000 INFO @ Sat, 15 Jan 2022 20:55:02: 12000000 INFO @ Sat, 15 Jan 2022 20:55:06: 1000000 INFO @ Sat, 15 Jan 2022 20:55:08: 13000000 INFO @ Sat, 15 Jan 2022 20:55:08: 6000000 INFO @ Sat, 15 Jan 2022 20:55:13: 14000000 INFO @ Sat, 15 Jan 2022 20:55:13: 2000000 INFO @ Sat, 15 Jan 2022 20:55:15: 7000000 INFO @ Sat, 15 Jan 2022 20:55:19: 15000000 INFO @ Sat, 15 Jan 2022 20:55:20: 3000000 INFO @ Sat, 15 Jan 2022 20:55:21: 8000000 INFO @ Sat, 15 Jan 2022 20:55:25: 16000000 INFO @ Sat, 15 Jan 2022 20:55:27: 4000000 INFO @ Sat, 15 Jan 2022 20:55:28: 9000000 INFO @ Sat, 15 Jan 2022 20:55:31: 17000000 INFO @ Sat, 15 Jan 2022 20:55:34: 5000000 INFO @ Sat, 15 Jan 2022 20:55:35: 10000000 INFO @ Sat, 15 Jan 2022 20:55:36: 18000000 INFO @ Sat, 15 Jan 2022 20:55:41: 6000000 INFO @ Sat, 15 Jan 2022 20:55:42: 11000000 INFO @ Sat, 15 Jan 2022 20:55:42: 19000000 INFO @ Sat, 15 Jan 2022 20:55:48: 7000000 INFO @ Sat, 15 Jan 2022 20:55:48: 12000000 INFO @ Sat, 15 Jan 2022 20:55:48: 20000000 INFO @ Sat, 15 Jan 2022 20:55:54: 13000000 INFO @ Sat, 15 Jan 2022 20:55:54: 21000000 INFO @ Sat, 15 Jan 2022 20:55:55: 8000000 INFO @ Sat, 15 Jan 2022 20:56:00: 14000000 INFO @ Sat, 15 Jan 2022 20:56:00: 22000000 INFO @ Sat, 15 Jan 2022 20:56:01: 9000000 INFO @ Sat, 15 Jan 2022 20:56:01: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 20:56:01: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 20:56:01: #1 total tags in treatment: 7218020 INFO @ Sat, 15 Jan 2022 20:56:01: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 20:56:01: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 20:56:01: #1 tags after filtering in treatment: 5802814 INFO @ Sat, 15 Jan 2022 20:56:01: #1 Redundant rate of treatment: 0.20 INFO @ Sat, 15 Jan 2022 20:56:01: #1 finished! INFO @ Sat, 15 Jan 2022 20:56:01: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 20:56:01: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 20:56:02: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 20:56:02: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 20:56:02: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8245480/SRX8245480.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245480/SRX8245480.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245480/SRX8245480.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245480/SRX8245480.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 20:56:06: 15000000 INFO @ Sat, 15 Jan 2022 20:56:08: 10000000 INFO @ Sat, 15 Jan 2022 20:56:11: 16000000 INFO @ Sat, 15 Jan 2022 20:56:14: 11000000 INFO @ Sat, 15 Jan 2022 20:56:17: 17000000 INFO @ Sat, 15 Jan 2022 20:56:21: 12000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 20:56:23: 18000000 INFO @ Sat, 15 Jan 2022 20:56:27: 13000000 INFO @ Sat, 15 Jan 2022 20:56:28: 19000000 INFO @ Sat, 15 Jan 2022 20:56:33: 14000000 INFO @ Sat, 15 Jan 2022 20:56:34: 20000000 BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 20:56:39: 21000000 INFO @ Sat, 15 Jan 2022 20:56:40: 15000000 INFO @ Sat, 15 Jan 2022 20:56:45: 22000000 INFO @ Sat, 15 Jan 2022 20:56:46: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 20:56:46: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 20:56:46: #1 total tags in treatment: 7218020 INFO @ Sat, 15 Jan 2022 20:56:46: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 20:56:46: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 20:56:46: #1 tags after filtering in treatment: 5802814 INFO @ Sat, 15 Jan 2022 20:56:46: #1 Redundant rate of treatment: 0.20 INFO @ Sat, 15 Jan 2022 20:56:46: #1 finished! INFO @ Sat, 15 Jan 2022 20:56:46: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 20:56:46: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 20:56:46: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 20:56:46: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 20:56:46: Process for pairing-model is terminated! INFO @ Sat, 15 Jan 2022 20:56:46: 16000000 cut: /home/okishinya/chipatlas/results/sacCer3/SRX8245480/SRX8245480.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245480/SRX8245480.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245480/SRX8245480.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245480/SRX8245480.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 20:56:52: 17000000 INFO @ Sat, 15 Jan 2022 20:56:58: 18000000 INFO @ Sat, 15 Jan 2022 20:57:04: 19000000 INFO @ Sat, 15 Jan 2022 20:57:10: 20000000 INFO @ Sat, 15 Jan 2022 20:57:17: 21000000 INFO @ Sat, 15 Jan 2022 20:57:23: 22000000 INFO @ Sat, 15 Jan 2022 20:57:24: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 20:57:24: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 20:57:24: #1 total tags in treatment: 7218020 INFO @ Sat, 15 Jan 2022 20:57:24: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 20:57:24: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 20:57:24: #1 tags after filtering in treatment: 5802814 INFO @ Sat, 15 Jan 2022 20:57:24: #1 Redundant rate of treatment: 0.20 INFO @ Sat, 15 Jan 2022 20:57:24: #1 finished! INFO @ Sat, 15 Jan 2022 20:57:24: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 20:57:24: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 20:57:25: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 20:57:25: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 20:57:25: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8245480/SRX8245480.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245480/SRX8245480.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245480/SRX8245480.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245480/SRX8245480.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling