Job ID = 14521110 SRX = SRX8245479 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 11357854 spots for SRR11684690/SRR11684690.sra Written 11357854 spots for SRR11684690/SRR11684690.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:01 Multiseed full-index search: 00:02:24 11357854 reads; of these: 11357854 (100.00%) were unpaired; of these: 359588 (3.17%) aligned 0 times 9906150 (87.22%) aligned exactly 1 time 1092116 (9.62%) aligned >1 times 96.83% overall alignment rate Time searching: 00:02:25 Overall time: 00:02:25 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 2758649 / 10998266 = 0.2508 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 20:33:14: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8245479/SRX8245479.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8245479/SRX8245479.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8245479/SRX8245479.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8245479/SRX8245479.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 20:33:14: #1 read tag files... INFO @ Sat, 15 Jan 2022 20:33:14: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 20:33:24: 1000000 INFO @ Sat, 15 Jan 2022 20:33:33: 2000000 INFO @ Sat, 15 Jan 2022 20:33:42: 3000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 20:33:45: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8245479/SRX8245479.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8245479/SRX8245479.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8245479/SRX8245479.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8245479/SRX8245479.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 20:33:45: #1 read tag files... INFO @ Sat, 15 Jan 2022 20:33:45: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 20:33:51: 4000000 INFO @ Sat, 15 Jan 2022 20:33:55: 1000000 INFO @ Sat, 15 Jan 2022 20:34:01: 5000000 INFO @ Sat, 15 Jan 2022 20:34:04: 2000000 INFO @ Sat, 15 Jan 2022 20:34:10: 6000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 20:34:14: 3000000 INFO @ Sat, 15 Jan 2022 20:34:15: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8245479/SRX8245479.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8245479/SRX8245479.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8245479/SRX8245479.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8245479/SRX8245479.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 20:34:15: #1 read tag files... INFO @ Sat, 15 Jan 2022 20:34:15: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 20:34:20: 7000000 INFO @ Sat, 15 Jan 2022 20:34:23: 4000000 INFO @ Sat, 15 Jan 2022 20:34:25: 1000000 INFO @ Sat, 15 Jan 2022 20:34:30: 8000000 INFO @ Sat, 15 Jan 2022 20:34:31: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 20:34:31: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 20:34:31: #1 total tags in treatment: 8239617 INFO @ Sat, 15 Jan 2022 20:34:31: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 20:34:31: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 20:34:32: #1 tags after filtering in treatment: 8239617 INFO @ Sat, 15 Jan 2022 20:34:32: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 20:34:32: #1 finished! INFO @ Sat, 15 Jan 2022 20:34:32: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 20:34:32: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 20:34:32: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 20:34:32: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 20:34:32: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8245479/SRX8245479.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245479/SRX8245479.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245479/SRX8245479.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245479/SRX8245479.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 20:34:32: 5000000 INFO @ Sat, 15 Jan 2022 20:34:34: 2000000 INFO @ Sat, 15 Jan 2022 20:34:42: 6000000 INFO @ Sat, 15 Jan 2022 20:34:44: 3000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 20:34:51: 7000000 INFO @ Sat, 15 Jan 2022 20:34:54: 4000000 INFO @ Sat, 15 Jan 2022 20:35:01: 8000000 BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 20:35:03: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 20:35:03: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 20:35:03: #1 total tags in treatment: 8239617 INFO @ Sat, 15 Jan 2022 20:35:03: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 20:35:03: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 20:35:03: #1 tags after filtering in treatment: 8239617 INFO @ Sat, 15 Jan 2022 20:35:03: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 20:35:03: #1 finished! INFO @ Sat, 15 Jan 2022 20:35:03: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 20:35:03: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 20:35:03: 5000000 INFO @ Sat, 15 Jan 2022 20:35:04: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 20:35:04: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 20:35:04: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8245479/SRX8245479.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245479/SRX8245479.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245479/SRX8245479.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245479/SRX8245479.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 20:35:13: 6000000 INFO @ Sat, 15 Jan 2022 20:35:22: 7000000 INFO @ Sat, 15 Jan 2022 20:35:32: 8000000 INFO @ Sat, 15 Jan 2022 20:35:33: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 20:35:33: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 20:35:33: #1 total tags in treatment: 8239617 INFO @ Sat, 15 Jan 2022 20:35:33: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 20:35:33: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 20:35:33: #1 tags after filtering in treatment: 8239617 INFO @ Sat, 15 Jan 2022 20:35:33: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 20:35:33: #1 finished! INFO @ Sat, 15 Jan 2022 20:35:33: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 20:35:33: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 20:35:34: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 20:35:34: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 20:35:34: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8245479/SRX8245479.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245479/SRX8245479.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245479/SRX8245479.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245479/SRX8245479.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling