Job ID = 14521109 SRX = SRX8245478 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 11372041 spots for SRR11684689/SRR11684689.sra Written 11372041 spots for SRR11684689/SRR11684689.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:01:30 11372041 reads; of these: 11372041 (100.00%) were unpaired; of these: 403686 (3.55%) aligned 0 times 9886376 (86.94%) aligned exactly 1 time 1081979 (9.51%) aligned >1 times 96.45% overall alignment rate Time searching: 00:01:30 Overall time: 00:01:30 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 2823483 / 10968355 = 0.2574 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 20:31:06: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8245478/SRX8245478.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8245478/SRX8245478.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8245478/SRX8245478.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8245478/SRX8245478.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 20:31:06: #1 read tag files... INFO @ Sat, 15 Jan 2022 20:31:06: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 20:31:11: 1000000 INFO @ Sat, 15 Jan 2022 20:31:15: 2000000 INFO @ Sat, 15 Jan 2022 20:31:20: 3000000 INFO @ Sat, 15 Jan 2022 20:31:24: 4000000 INFO @ Sat, 15 Jan 2022 20:31:29: 5000000 INFO @ Sat, 15 Jan 2022 20:31:33: 6000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 20:31:36: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8245478/SRX8245478.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8245478/SRX8245478.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8245478/SRX8245478.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8245478/SRX8245478.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 20:31:36: #1 read tag files... INFO @ Sat, 15 Jan 2022 20:31:36: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 20:31:38: 7000000 INFO @ Sat, 15 Jan 2022 20:31:42: 1000000 INFO @ Sat, 15 Jan 2022 20:31:43: 8000000 INFO @ Sat, 15 Jan 2022 20:31:43: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 20:31:43: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 20:31:43: #1 total tags in treatment: 8144872 INFO @ Sat, 15 Jan 2022 20:31:43: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 20:31:43: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 20:31:43: #1 tags after filtering in treatment: 8144872 INFO @ Sat, 15 Jan 2022 20:31:43: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 20:31:43: #1 finished! INFO @ Sat, 15 Jan 2022 20:31:43: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 20:31:43: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 20:31:44: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 20:31:44: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 20:31:44: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8245478/SRX8245478.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245478/SRX8245478.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245478/SRX8245478.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245478/SRX8245478.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 20:31:47: 2000000 INFO @ Sat, 15 Jan 2022 20:31:53: 3000000 INFO @ Sat, 15 Jan 2022 20:31:58: 4000000 INFO @ Sat, 15 Jan 2022 20:32:03: 5000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 20:32:06: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8245478/SRX8245478.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8245478/SRX8245478.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8245478/SRX8245478.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8245478/SRX8245478.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 20:32:06: #1 read tag files... INFO @ Sat, 15 Jan 2022 20:32:06: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 20:32:08: 6000000 INFO @ Sat, 15 Jan 2022 20:32:12: 1000000 INFO @ Sat, 15 Jan 2022 20:32:14: 7000000 INFO @ Sat, 15 Jan 2022 20:32:17: 2000000 INFO @ Sat, 15 Jan 2022 20:32:19: 8000000 INFO @ Sat, 15 Jan 2022 20:32:20: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 20:32:20: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 20:32:20: #1 total tags in treatment: 8144872 INFO @ Sat, 15 Jan 2022 20:32:20: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 20:32:20: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 20:32:20: #1 tags after filtering in treatment: 8144872 INFO @ Sat, 15 Jan 2022 20:32:20: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 20:32:20: #1 finished! INFO @ Sat, 15 Jan 2022 20:32:20: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 20:32:20: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 20:32:21: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 20:32:21: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 20:32:21: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8245478/SRX8245478.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245478/SRX8245478.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245478/SRX8245478.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245478/SRX8245478.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 20:32:23: 3000000 INFO @ Sat, 15 Jan 2022 20:32:28: 4000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 20:32:33: 5000000 INFO @ Sat, 15 Jan 2022 20:32:38: 6000000 BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 20:32:43: 7000000 INFO @ Sat, 15 Jan 2022 20:32:49: 8000000 INFO @ Sat, 15 Jan 2022 20:32:49: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 20:32:49: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 20:32:49: #1 total tags in treatment: 8144872 INFO @ Sat, 15 Jan 2022 20:32:49: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 20:32:49: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 20:32:49: #1 tags after filtering in treatment: 8144872 INFO @ Sat, 15 Jan 2022 20:32:49: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 20:32:49: #1 finished! INFO @ Sat, 15 Jan 2022 20:32:49: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 20:32:49: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 20:32:50: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 20:32:50: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 20:32:50: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8245478/SRX8245478.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245478/SRX8245478.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245478/SRX8245478.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245478/SRX8245478.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling