Job ID = 14521108 SRX = SRX8245477 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 15961161 spots for SRR11684688/SRR11684688.sra Written 15961161 spots for SRR11684688/SRR11684688.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:02:11 15961161 reads; of these: 15961161 (100.00%) were unpaired; of these: 595914 (3.73%) aligned 0 times 13783579 (86.36%) aligned exactly 1 time 1581668 (9.91%) aligned >1 times 96.27% overall alignment rate Time searching: 00:02:11 Overall time: 00:02:11 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 4982804 / 15365247 = 0.3243 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 20:33:15: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8245477/SRX8245477.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8245477/SRX8245477.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8245477/SRX8245477.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8245477/SRX8245477.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 20:33:15: #1 read tag files... INFO @ Sat, 15 Jan 2022 20:33:15: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 20:33:20: 1000000 INFO @ Sat, 15 Jan 2022 20:33:25: 2000000 INFO @ Sat, 15 Jan 2022 20:33:30: 3000000 INFO @ Sat, 15 Jan 2022 20:33:34: 4000000 INFO @ Sat, 15 Jan 2022 20:33:39: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 20:33:44: 6000000 INFO @ Sat, 15 Jan 2022 20:33:45: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8245477/SRX8245477.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8245477/SRX8245477.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8245477/SRX8245477.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8245477/SRX8245477.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 20:33:45: #1 read tag files... INFO @ Sat, 15 Jan 2022 20:33:45: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 20:33:49: 7000000 INFO @ Sat, 15 Jan 2022 20:33:50: 1000000 INFO @ Sat, 15 Jan 2022 20:33:54: 8000000 INFO @ Sat, 15 Jan 2022 20:33:55: 2000000 INFO @ Sat, 15 Jan 2022 20:33:59: 9000000 INFO @ Sat, 15 Jan 2022 20:34:00: 3000000 INFO @ Sat, 15 Jan 2022 20:34:04: 10000000 INFO @ Sat, 15 Jan 2022 20:34:06: 4000000 INFO @ Sat, 15 Jan 2022 20:34:06: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 20:34:06: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 20:34:06: #1 total tags in treatment: 10382443 INFO @ Sat, 15 Jan 2022 20:34:06: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 20:34:06: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 20:34:06: #1 tags after filtering in treatment: 10382443 INFO @ Sat, 15 Jan 2022 20:34:06: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 20:34:06: #1 finished! INFO @ Sat, 15 Jan 2022 20:34:06: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 20:34:06: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 20:34:07: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 20:34:07: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 20:34:07: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8245477/SRX8245477.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245477/SRX8245477.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245477/SRX8245477.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245477/SRX8245477.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 20:34:10: 5000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 20:34:15: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8245477/SRX8245477.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8245477/SRX8245477.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8245477/SRX8245477.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8245477/SRX8245477.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 20:34:15: #1 read tag files... INFO @ Sat, 15 Jan 2022 20:34:15: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 20:34:15: 6000000 INFO @ Sat, 15 Jan 2022 20:34:20: 7000000 INFO @ Sat, 15 Jan 2022 20:34:20: 1000000 INFO @ Sat, 15 Jan 2022 20:34:25: 2000000 INFO @ Sat, 15 Jan 2022 20:34:25: 8000000 INFO @ Sat, 15 Jan 2022 20:34:30: 3000000 INFO @ Sat, 15 Jan 2022 20:34:31: 9000000 INFO @ Sat, 15 Jan 2022 20:34:35: 4000000 INFO @ Sat, 15 Jan 2022 20:34:36: 10000000 INFO @ Sat, 15 Jan 2022 20:34:38: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 20:34:38: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 20:34:38: #1 total tags in treatment: 10382443 INFO @ Sat, 15 Jan 2022 20:34:38: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 20:34:38: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 20:34:38: #1 tags after filtering in treatment: 10382443 INFO @ Sat, 15 Jan 2022 20:34:38: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 20:34:38: #1 finished! INFO @ Sat, 15 Jan 2022 20:34:38: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 20:34:38: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 20:34:38: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 20:34:38: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 20:34:38: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8245477/SRX8245477.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245477/SRX8245477.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245477/SRX8245477.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245477/SRX8245477.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 20:34:40: 5000000 INFO @ Sat, 15 Jan 2022 20:34:45: 6000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 20:34:50: 7000000 INFO @ Sat, 15 Jan 2022 20:34:55: 8000000 BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 20:35:00: 9000000 INFO @ Sat, 15 Jan 2022 20:35:05: 10000000 INFO @ Sat, 15 Jan 2022 20:35:06: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 20:35:06: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 20:35:06: #1 total tags in treatment: 10382443 INFO @ Sat, 15 Jan 2022 20:35:06: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 20:35:06: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 20:35:06: #1 tags after filtering in treatment: 10382443 INFO @ Sat, 15 Jan 2022 20:35:06: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 20:35:06: #1 finished! INFO @ Sat, 15 Jan 2022 20:35:06: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 20:35:06: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 20:35:07: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 20:35:07: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 20:35:07: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8245477/SRX8245477.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245477/SRX8245477.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245477/SRX8245477.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245477/SRX8245477.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling