Job ID = 14521106 SRX = SRX8245475 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 15214305 spots for SRR11684686/SRR11684686.sra Written 15214305 spots for SRR11684686/SRR11684686.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:08:33 15214305 reads; of these: 15214305 (100.00%) were paired; of these: 6100646 (40.10%) aligned concordantly 0 times 8276182 (54.40%) aligned concordantly exactly 1 time 837477 (5.50%) aligned concordantly >1 times ---- 6100646 pairs aligned concordantly 0 times; of these: 256031 (4.20%) aligned discordantly 1 time ---- 5844615 pairs aligned 0 times concordantly or discordantly; of these: 11689230 mates make up the pairs; of these: 6341294 (54.25%) aligned 0 times 4752664 (40.66%) aligned exactly 1 time 595272 (5.09%) aligned >1 times 79.16% overall alignment rate Time searching: 00:08:33 Overall time: 00:08:33 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 12 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 80862 / 9367712 = 0.0086 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 20:45:54: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8245475/SRX8245475.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8245475/SRX8245475.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8245475/SRX8245475.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8245475/SRX8245475.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 20:45:54: #1 read tag files... INFO @ Sat, 15 Jan 2022 20:45:54: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 20:45:59: 1000000 INFO @ Sat, 15 Jan 2022 20:46:04: 2000000 INFO @ Sat, 15 Jan 2022 20:46:10: 3000000 INFO @ Sat, 15 Jan 2022 20:46:15: 4000000 INFO @ Sat, 15 Jan 2022 20:46:20: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 20:46:24: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8245475/SRX8245475.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8245475/SRX8245475.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8245475/SRX8245475.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8245475/SRX8245475.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 20:46:24: #1 read tag files... INFO @ Sat, 15 Jan 2022 20:46:24: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 20:46:25: 6000000 INFO @ Sat, 15 Jan 2022 20:46:31: 7000000 INFO @ Sat, 15 Jan 2022 20:46:31: 1000000 INFO @ Sat, 15 Jan 2022 20:46:37: 8000000 INFO @ Sat, 15 Jan 2022 20:46:38: 2000000 INFO @ Sat, 15 Jan 2022 20:46:44: 9000000 INFO @ Sat, 15 Jan 2022 20:46:44: 3000000 INFO @ Sat, 15 Jan 2022 20:46:51: 10000000 INFO @ Sat, 15 Jan 2022 20:46:51: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 20:46:54: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8245475/SRX8245475.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8245475/SRX8245475.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8245475/SRX8245475.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8245475/SRX8245475.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 20:46:54: #1 read tag files... INFO @ Sat, 15 Jan 2022 20:46:54: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 20:46:58: 11000000 INFO @ Sat, 15 Jan 2022 20:46:59: 5000000 INFO @ Sat, 15 Jan 2022 20:47:01: 1000000 INFO @ Sat, 15 Jan 2022 20:47:04: 12000000 INFO @ Sat, 15 Jan 2022 20:47:06: 6000000 INFO @ Sat, 15 Jan 2022 20:47:07: 2000000 INFO @ Sat, 15 Jan 2022 20:47:11: 13000000 INFO @ Sat, 15 Jan 2022 20:47:14: 7000000 INFO @ Sat, 15 Jan 2022 20:47:14: 3000000 INFO @ Sat, 15 Jan 2022 20:47:17: 14000000 INFO @ Sat, 15 Jan 2022 20:47:20: 4000000 INFO @ Sat, 15 Jan 2022 20:47:21: 8000000 INFO @ Sat, 15 Jan 2022 20:47:23: 15000000 INFO @ Sat, 15 Jan 2022 20:47:27: 5000000 INFO @ Sat, 15 Jan 2022 20:47:28: 9000000 INFO @ Sat, 15 Jan 2022 20:47:30: 16000000 INFO @ Sat, 15 Jan 2022 20:47:33: 6000000 INFO @ Sat, 15 Jan 2022 20:47:34: 10000000 INFO @ Sat, 15 Jan 2022 20:47:36: 17000000 INFO @ Sat, 15 Jan 2022 20:47:40: 7000000 INFO @ Sat, 15 Jan 2022 20:47:41: 11000000 INFO @ Sat, 15 Jan 2022 20:47:43: 18000000 INFO @ Sat, 15 Jan 2022 20:47:47: 8000000 INFO @ Sat, 15 Jan 2022 20:47:48: 12000000 INFO @ Sat, 15 Jan 2022 20:47:49: 19000000 INFO @ Sat, 15 Jan 2022 20:47:54: 9000000 INFO @ Sat, 15 Jan 2022 20:47:55: 13000000 INFO @ Sat, 15 Jan 2022 20:47:55: 20000000 INFO @ Sat, 15 Jan 2022 20:48:01: 10000000 INFO @ Sat, 15 Jan 2022 20:48:02: 21000000 INFO @ Sat, 15 Jan 2022 20:48:02: 14000000 INFO @ Sat, 15 Jan 2022 20:48:07: 11000000 INFO @ Sat, 15 Jan 2022 20:48:08: 22000000 INFO @ Sat, 15 Jan 2022 20:48:10: 15000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 20:48:15: 12000000 INFO @ Sat, 15 Jan 2022 20:48:15: 23000000 INFO @ Sat, 15 Jan 2022 20:48:17: 16000000 INFO @ Sat, 15 Jan 2022 20:48:21: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 20:48:21: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 20:48:21: #1 total tags in treatment: 9033149 INFO @ Sat, 15 Jan 2022 20:48:21: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 20:48:21: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 20:48:21: #1 tags after filtering in treatment: 6846785 INFO @ Sat, 15 Jan 2022 20:48:21: #1 Redundant rate of treatment: 0.24 INFO @ Sat, 15 Jan 2022 20:48:21: #1 finished! INFO @ Sat, 15 Jan 2022 20:48:21: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 20:48:21: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 20:48:21: 13000000 INFO @ Sat, 15 Jan 2022 20:48:21: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 20:48:21: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 20:48:21: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8245475/SRX8245475.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245475/SRX8245475.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245475/SRX8245475.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245475/SRX8245475.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 20:48:24: 17000000 INFO @ Sat, 15 Jan 2022 20:48:27: 14000000 BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 20:48:32: 18000000 INFO @ Sat, 15 Jan 2022 20:48:34: 15000000 INFO @ Sat, 15 Jan 2022 20:48:39: 19000000 INFO @ Sat, 15 Jan 2022 20:48:40: 16000000 INFO @ Sat, 15 Jan 2022 20:48:47: 20000000 INFO @ Sat, 15 Jan 2022 20:48:47: 17000000 INFO @ Sat, 15 Jan 2022 20:48:53: 18000000 INFO @ Sat, 15 Jan 2022 20:48:54: 21000000 INFO @ Sat, 15 Jan 2022 20:48:59: 19000000 INFO @ Sat, 15 Jan 2022 20:49:01: 22000000 INFO @ Sat, 15 Jan 2022 20:49:06: 20000000 INFO @ Sat, 15 Jan 2022 20:49:09: 23000000 INFO @ Sat, 15 Jan 2022 20:49:12: 21000000 INFO @ Sat, 15 Jan 2022 20:49:15: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 20:49:15: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 20:49:15: #1 total tags in treatment: 9033149 INFO @ Sat, 15 Jan 2022 20:49:15: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 20:49:15: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 20:49:16: #1 tags after filtering in treatment: 6846785 INFO @ Sat, 15 Jan 2022 20:49:16: #1 Redundant rate of treatment: 0.24 INFO @ Sat, 15 Jan 2022 20:49:16: #1 finished! INFO @ Sat, 15 Jan 2022 20:49:16: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 20:49:16: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 20:49:16: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 20:49:16: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 20:49:16: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8245475/SRX8245475.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 554 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245475/SRX8245475.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245475/SRX8245475.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245475/SRX8245475.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 20:49:18: 22000000 INFO @ Sat, 15 Jan 2022 20:49:24: 23000000 INFO @ Sat, 15 Jan 2022 20:49:28: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 20:49:28: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 20:49:28: #1 total tags in treatment: 9033149 INFO @ Sat, 15 Jan 2022 20:49:28: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 20:49:28: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 20:49:29: #1 tags after filtering in treatment: 6846785 INFO @ Sat, 15 Jan 2022 20:49:29: #1 Redundant rate of treatment: 0.24 INFO @ Sat, 15 Jan 2022 20:49:29: #1 finished! INFO @ Sat, 15 Jan 2022 20:49:29: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 20:49:29: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 20:49:29: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 20:49:29: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 20:49:29: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8245475/SRX8245475.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245475/SRX8245475.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245475/SRX8245475.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245475/SRX8245475.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling