Job ID = 14521105 SRX = SRX8245474 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 9841766 spots for SRR11684685/SRR11684685.sra Written 9841766 spots for SRR11684685/SRR11684685.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:08:11 9841766 reads; of these: 9841766 (100.00%) were paired; of these: 3434960 (34.90%) aligned concordantly 0 times 5850255 (59.44%) aligned concordantly exactly 1 time 556551 (5.65%) aligned concordantly >1 times ---- 3434960 pairs aligned concordantly 0 times; of these: 134938 (3.93%) aligned discordantly 1 time ---- 3300022 pairs aligned 0 times concordantly or discordantly; of these: 6600044 mates make up the pairs; of these: 3403774 (51.57%) aligned 0 times 2859985 (43.33%) aligned exactly 1 time 336285 (5.10%) aligned >1 times 82.71% overall alignment rate Time searching: 00:08:11 Overall time: 00:08:11 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 8 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 43316 / 6540132 = 0.0066 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 20:43:02: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8245474/SRX8245474.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8245474/SRX8245474.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8245474/SRX8245474.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8245474/SRX8245474.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 20:43:02: #1 read tag files... INFO @ Sat, 15 Jan 2022 20:43:02: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 20:43:09: 1000000 INFO @ Sat, 15 Jan 2022 20:43:15: 2000000 INFO @ Sat, 15 Jan 2022 20:43:21: 3000000 INFO @ Sat, 15 Jan 2022 20:43:26: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 20:43:32: 5000000 INFO @ Sat, 15 Jan 2022 20:43:32: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8245474/SRX8245474.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8245474/SRX8245474.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8245474/SRX8245474.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8245474/SRX8245474.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 20:43:32: #1 read tag files... INFO @ Sat, 15 Jan 2022 20:43:32: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 20:43:38: 6000000 INFO @ Sat, 15 Jan 2022 20:43:38: 1000000 INFO @ Sat, 15 Jan 2022 20:43:44: 7000000 INFO @ Sat, 15 Jan 2022 20:43:44: 2000000 INFO @ Sat, 15 Jan 2022 20:43:50: 8000000 INFO @ Sat, 15 Jan 2022 20:43:50: 3000000 INFO @ Sat, 15 Jan 2022 20:43:56: 9000000 INFO @ Sat, 15 Jan 2022 20:43:56: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 20:44:02: 10000000 INFO @ Sat, 15 Jan 2022 20:44:02: 5000000 INFO @ Sat, 15 Jan 2022 20:44:02: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8245474/SRX8245474.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8245474/SRX8245474.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8245474/SRX8245474.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8245474/SRX8245474.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 20:44:02: #1 read tag files... INFO @ Sat, 15 Jan 2022 20:44:02: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 20:44:09: 11000000 INFO @ Sat, 15 Jan 2022 20:44:09: 6000000 INFO @ Sat, 15 Jan 2022 20:44:09: 1000000 INFO @ Sat, 15 Jan 2022 20:44:15: 12000000 INFO @ Sat, 15 Jan 2022 20:44:15: 7000000 INFO @ Sat, 15 Jan 2022 20:44:16: 2000000 INFO @ Sat, 15 Jan 2022 20:44:21: 13000000 INFO @ Sat, 15 Jan 2022 20:44:22: 8000000 INFO @ Sat, 15 Jan 2022 20:44:22: 3000000 INFO @ Sat, 15 Jan 2022 20:44:28: 14000000 INFO @ Sat, 15 Jan 2022 20:44:28: 9000000 INFO @ Sat, 15 Jan 2022 20:44:28: 4000000 INFO @ Sat, 15 Jan 2022 20:44:35: 15000000 INFO @ Sat, 15 Jan 2022 20:44:35: 5000000 INFO @ Sat, 15 Jan 2022 20:44:35: 10000000 INFO @ Sat, 15 Jan 2022 20:44:41: 16000000 INFO @ Sat, 15 Jan 2022 20:44:41: 6000000 INFO @ Sat, 15 Jan 2022 20:44:41: 11000000 INFO @ Sat, 15 Jan 2022 20:44:42: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 20:44:42: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 20:44:42: #1 total tags in treatment: 6363667 INFO @ Sat, 15 Jan 2022 20:44:42: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 20:44:42: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 20:44:43: #1 tags after filtering in treatment: 5254844 INFO @ Sat, 15 Jan 2022 20:44:43: #1 Redundant rate of treatment: 0.17 INFO @ Sat, 15 Jan 2022 20:44:43: #1 finished! INFO @ Sat, 15 Jan 2022 20:44:43: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 20:44:43: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 20:44:43: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 20:44:43: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 20:44:43: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8245474/SRX8245474.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 92 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245474/SRX8245474.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245474/SRX8245474.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245474/SRX8245474.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 20:44:47: 7000000 INFO @ Sat, 15 Jan 2022 20:44:47: 12000000 INFO @ Sat, 15 Jan 2022 20:44:53: 8000000 INFO @ Sat, 15 Jan 2022 20:44:53: 13000000 INFO @ Sat, 15 Jan 2022 20:44:59: 9000000 INFO @ Sat, 15 Jan 2022 20:44:59: 14000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 20:45:05: 10000000 INFO @ Sat, 15 Jan 2022 20:45:05: 15000000 INFO @ Sat, 15 Jan 2022 20:45:11: 11000000 INFO @ Sat, 15 Jan 2022 20:45:12: 16000000 INFO @ Sat, 15 Jan 2022 20:45:13: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 20:45:13: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 20:45:13: #1 total tags in treatment: 6363667 INFO @ Sat, 15 Jan 2022 20:45:13: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 20:45:13: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 20:45:13: #1 tags after filtering in treatment: 5254844 INFO @ Sat, 15 Jan 2022 20:45:13: #1 Redundant rate of treatment: 0.17 INFO @ Sat, 15 Jan 2022 20:45:13: #1 finished! INFO @ Sat, 15 Jan 2022 20:45:13: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 20:45:13: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 20:45:13: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 20:45:13: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 20:45:13: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8245474/SRX8245474.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245474/SRX8245474.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245474/SRX8245474.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245474/SRX8245474.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 20:45:16: 12000000 BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 20:45:22: 13000000 INFO @ Sat, 15 Jan 2022 20:45:27: 14000000 INFO @ Sat, 15 Jan 2022 20:45:32: 15000000 INFO @ Sat, 15 Jan 2022 20:45:38: 16000000 INFO @ Sat, 15 Jan 2022 20:45:39: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 20:45:39: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 20:45:39: #1 total tags in treatment: 6363667 INFO @ Sat, 15 Jan 2022 20:45:39: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 20:45:39: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 20:45:39: #1 tags after filtering in treatment: 5254844 INFO @ Sat, 15 Jan 2022 20:45:39: #1 Redundant rate of treatment: 0.17 INFO @ Sat, 15 Jan 2022 20:45:39: #1 finished! INFO @ Sat, 15 Jan 2022 20:45:39: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 20:45:39: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 20:45:39: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 20:45:39: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 20:45:39: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8245474/SRX8245474.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245474/SRX8245474.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245474/SRX8245474.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245474/SRX8245474.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling