Job ID = 14521103 SRX = SRX8245472 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 9722834 spots for SRR11684683/SRR11684683.sra Written 9722834 spots for SRR11684683/SRR11684683.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:09:45 9722834 reads; of these: 9722834 (100.00%) were paired; of these: 5437281 (55.92%) aligned concordantly 0 times 3699999 (38.05%) aligned concordantly exactly 1 time 585554 (6.02%) aligned concordantly >1 times ---- 5437281 pairs aligned concordantly 0 times; of these: 362003 (6.66%) aligned discordantly 1 time ---- 5075278 pairs aligned 0 times concordantly or discordantly; of these: 10150556 mates make up the pairs; of these: 6076126 (59.86%) aligned 0 times 3337380 (32.88%) aligned exactly 1 time 737050 (7.26%) aligned >1 times 68.75% overall alignment rate Time searching: 00:09:45 Overall time: 00:09:45 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 8 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 162455 / 4645975 = 0.0350 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 20:42:09: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8245472/SRX8245472.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8245472/SRX8245472.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8245472/SRX8245472.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8245472/SRX8245472.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 20:42:09: #1 read tag files... INFO @ Sat, 15 Jan 2022 20:42:09: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 20:42:13: 1000000 INFO @ Sat, 15 Jan 2022 20:42:17: 2000000 INFO @ Sat, 15 Jan 2022 20:42:21: 3000000 INFO @ Sat, 15 Jan 2022 20:42:26: 4000000 INFO @ Sat, 15 Jan 2022 20:42:30: 5000000 INFO @ Sat, 15 Jan 2022 20:42:34: 6000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 20:42:38: 7000000 INFO @ Sat, 15 Jan 2022 20:42:39: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8245472/SRX8245472.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8245472/SRX8245472.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8245472/SRX8245472.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8245472/SRX8245472.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 20:42:39: #1 read tag files... INFO @ Sat, 15 Jan 2022 20:42:39: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 20:42:42: 8000000 INFO @ Sat, 15 Jan 2022 20:42:43: 1000000 INFO @ Sat, 15 Jan 2022 20:42:46: 9000000 INFO @ Sat, 15 Jan 2022 20:42:47: 2000000 INFO @ Sat, 15 Jan 2022 20:42:50: 10000000 INFO @ Sat, 15 Jan 2022 20:42:51: 3000000 INFO @ Sat, 15 Jan 2022 20:42:54: 11000000 INFO @ Sat, 15 Jan 2022 20:42:56: 4000000 INFO @ Sat, 15 Jan 2022 20:42:59: 12000000 INFO @ Sat, 15 Jan 2022 20:43:00: 5000000 INFO @ Sat, 15 Jan 2022 20:43:03: 13000000 INFO @ Sat, 15 Jan 2022 20:43:03: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 20:43:03: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 20:43:03: #1 total tags in treatment: 4129012 INFO @ Sat, 15 Jan 2022 20:43:03: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 20:43:03: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 20:43:03: #1 tags after filtering in treatment: 3367893 INFO @ Sat, 15 Jan 2022 20:43:03: #1 Redundant rate of treatment: 0.18 INFO @ Sat, 15 Jan 2022 20:43:03: #1 finished! INFO @ Sat, 15 Jan 2022 20:43:03: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 20:43:03: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 20:43:03: #2 number of paired peaks: 38 WARNING @ Sat, 15 Jan 2022 20:43:03: Too few paired peaks (38) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 20:43:03: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8245472/SRX8245472.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245472/SRX8245472.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245472/SRX8245472.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245472/SRX8245472.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 20:43:04: 6000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 20:43:08: 7000000 INFO @ Sat, 15 Jan 2022 20:43:09: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8245472/SRX8245472.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8245472/SRX8245472.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8245472/SRX8245472.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8245472/SRX8245472.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 20:43:09: #1 read tag files... INFO @ Sat, 15 Jan 2022 20:43:09: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 20:43:12: 8000000 INFO @ Sat, 15 Jan 2022 20:43:13: 1000000 INFO @ Sat, 15 Jan 2022 20:43:16: 9000000 INFO @ Sat, 15 Jan 2022 20:43:17: 2000000 INFO @ Sat, 15 Jan 2022 20:43:20: 10000000 INFO @ Sat, 15 Jan 2022 20:43:21: 3000000 INFO @ Sat, 15 Jan 2022 20:43:25: 11000000 INFO @ Sat, 15 Jan 2022 20:43:26: 4000000 INFO @ Sat, 15 Jan 2022 20:43:29: 12000000 INFO @ Sat, 15 Jan 2022 20:43:30: 5000000 INFO @ Sat, 15 Jan 2022 20:43:33: 13000000 INFO @ Sat, 15 Jan 2022 20:43:33: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 20:43:33: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 20:43:33: #1 total tags in treatment: 4129012 INFO @ Sat, 15 Jan 2022 20:43:33: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 20:43:33: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 20:43:33: #1 tags after filtering in treatment: 3367893 INFO @ Sat, 15 Jan 2022 20:43:33: #1 Redundant rate of treatment: 0.18 INFO @ Sat, 15 Jan 2022 20:43:33: #1 finished! INFO @ Sat, 15 Jan 2022 20:43:33: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 20:43:33: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 20:43:34: #2 number of paired peaks: 38 WARNING @ Sat, 15 Jan 2022 20:43:34: Too few paired peaks (38) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 20:43:34: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8245472/SRX8245472.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245472/SRX8245472.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245472/SRX8245472.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245472/SRX8245472.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 20:43:34: 6000000 INFO @ Sat, 15 Jan 2022 20:43:38: 7000000 INFO @ Sat, 15 Jan 2022 20:43:42: 8000000 INFO @ Sat, 15 Jan 2022 20:43:46: 9000000 INFO @ Sat, 15 Jan 2022 20:43:51: 10000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 20:43:55: 11000000 INFO @ Sat, 15 Jan 2022 20:43:59: 12000000 INFO @ Sat, 15 Jan 2022 20:44:03: 13000000 BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 20:44:03: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 20:44:03: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 20:44:03: #1 total tags in treatment: 4129012 INFO @ Sat, 15 Jan 2022 20:44:03: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 20:44:03: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 20:44:03: #1 tags after filtering in treatment: 3367893 INFO @ Sat, 15 Jan 2022 20:44:03: #1 Redundant rate of treatment: 0.18 INFO @ Sat, 15 Jan 2022 20:44:03: #1 finished! INFO @ Sat, 15 Jan 2022 20:44:03: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 20:44:03: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 20:44:03: #2 number of paired peaks: 38 WARNING @ Sat, 15 Jan 2022 20:44:03: Too few paired peaks (38) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 20:44:03: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8245472/SRX8245472.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245472/SRX8245472.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245472/SRX8245472.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245472/SRX8245472.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling