Job ID = 14521101 SRX = SRX8245470 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 15580643 spots for SRR11684681/SRR11684681.sra Written 15580643 spots for SRR11684681/SRR11684681.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:02:45 15580643 reads; of these: 15580643 (100.00%) were unpaired; of these: 765661 (4.91%) aligned 0 times 12280279 (78.82%) aligned exactly 1 time 2534703 (16.27%) aligned >1 times 95.09% overall alignment rate Time searching: 00:02:45 Overall time: 00:02:45 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 5559090 / 14814982 = 0.3752 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 20:33:37: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8245470/SRX8245470.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8245470/SRX8245470.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8245470/SRX8245470.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8245470/SRX8245470.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 20:33:37: #1 read tag files... INFO @ Sat, 15 Jan 2022 20:33:37: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 20:33:45: 1000000 INFO @ Sat, 15 Jan 2022 20:33:53: 2000000 INFO @ Sat, 15 Jan 2022 20:34:00: 3000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 20:34:07: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8245470/SRX8245470.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8245470/SRX8245470.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8245470/SRX8245470.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8245470/SRX8245470.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 20:34:07: #1 read tag files... INFO @ Sat, 15 Jan 2022 20:34:07: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 20:34:08: 4000000 INFO @ Sat, 15 Jan 2022 20:34:16: 1000000 INFO @ Sat, 15 Jan 2022 20:34:16: 5000000 INFO @ Sat, 15 Jan 2022 20:34:24: 6000000 INFO @ Sat, 15 Jan 2022 20:34:25: 2000000 INFO @ Sat, 15 Jan 2022 20:34:32: 7000000 INFO @ Sat, 15 Jan 2022 20:34:33: 3000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 20:34:37: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8245470/SRX8245470.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8245470/SRX8245470.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8245470/SRX8245470.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8245470/SRX8245470.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 20:34:37: #1 read tag files... INFO @ Sat, 15 Jan 2022 20:34:37: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 20:34:40: 8000000 INFO @ Sat, 15 Jan 2022 20:34:41: 4000000 INFO @ Sat, 15 Jan 2022 20:34:45: 1000000 INFO @ Sat, 15 Jan 2022 20:34:48: 9000000 INFO @ Sat, 15 Jan 2022 20:34:49: 5000000 INFO @ Sat, 15 Jan 2022 20:34:50: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 20:34:50: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 20:34:50: #1 total tags in treatment: 9255892 INFO @ Sat, 15 Jan 2022 20:34:50: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 20:34:50: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 20:34:50: #1 tags after filtering in treatment: 9255892 INFO @ Sat, 15 Jan 2022 20:34:50: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 20:34:50: #1 finished! INFO @ Sat, 15 Jan 2022 20:34:50: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 20:34:50: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 20:34:51: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 20:34:51: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 20:34:51: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8245470/SRX8245470.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245470/SRX8245470.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245470/SRX8245470.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245470/SRX8245470.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 20:34:53: 2000000 INFO @ Sat, 15 Jan 2022 20:34:57: 6000000 INFO @ Sat, 15 Jan 2022 20:35:01: 3000000 INFO @ Sat, 15 Jan 2022 20:35:05: 7000000 INFO @ Sat, 15 Jan 2022 20:35:09: 4000000 INFO @ Sat, 15 Jan 2022 20:35:12: 8000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 20:35:16: 5000000 INFO @ Sat, 15 Jan 2022 20:35:20: 9000000 INFO @ Sat, 15 Jan 2022 20:35:22: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 20:35:22: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 20:35:22: #1 total tags in treatment: 9255892 INFO @ Sat, 15 Jan 2022 20:35:22: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 20:35:22: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 20:35:22: #1 tags after filtering in treatment: 9255892 INFO @ Sat, 15 Jan 2022 20:35:22: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 20:35:22: #1 finished! INFO @ Sat, 15 Jan 2022 20:35:22: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 20:35:22: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 20:35:23: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 20:35:23: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 20:35:23: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8245470/SRX8245470.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245470/SRX8245470.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245470/SRX8245470.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245470/SRX8245470.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 20:35:24: 6000000 BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 20:35:31: 7000000 INFO @ Sat, 15 Jan 2022 20:35:39: 8000000 INFO @ Sat, 15 Jan 2022 20:35:47: 9000000 INFO @ Sat, 15 Jan 2022 20:35:48: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 20:35:48: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 20:35:48: #1 total tags in treatment: 9255892 INFO @ Sat, 15 Jan 2022 20:35:48: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 20:35:48: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 20:35:49: #1 tags after filtering in treatment: 9255892 INFO @ Sat, 15 Jan 2022 20:35:49: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 20:35:49: #1 finished! INFO @ Sat, 15 Jan 2022 20:35:49: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 20:35:49: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 20:35:49: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 20:35:49: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 20:35:49: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8245470/SRX8245470.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245470/SRX8245470.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245470/SRX8245470.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245470/SRX8245470.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling