Job ID = 14521043 SRX = SRX8245464 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 17021957 spots for SRR11684675/SRR11684675.sra Written 17021957 spots for SRR11684675/SRR11684675.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:12:33 17021957 reads; of these: 17021957 (100.00%) were paired; of these: 3148178 (18.49%) aligned concordantly 0 times 12305188 (72.29%) aligned concordantly exactly 1 time 1568591 (9.22%) aligned concordantly >1 times ---- 3148178 pairs aligned concordantly 0 times; of these: 28880 (0.92%) aligned discordantly 1 time ---- 3119298 pairs aligned 0 times concordantly or discordantly; of these: 6238596 mates make up the pairs; of these: 4686473 (75.12%) aligned 0 times 1353861 (21.70%) aligned exactly 1 time 198262 (3.18%) aligned >1 times 86.23% overall alignment rate Time searching: 00:12:34 Overall time: 00:12:34 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 12 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 1663055 / 13901069 = 0.1196 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 20:43:52: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8245464/SRX8245464.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8245464/SRX8245464.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8245464/SRX8245464.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8245464/SRX8245464.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 20:43:52: #1 read tag files... INFO @ Sat, 15 Jan 2022 20:43:52: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 20:43:57: 1000000 INFO @ Sat, 15 Jan 2022 20:44:03: 2000000 INFO @ Sat, 15 Jan 2022 20:44:09: 3000000 INFO @ Sat, 15 Jan 2022 20:44:14: 4000000 INFO @ Sat, 15 Jan 2022 20:44:19: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 20:44:22: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8245464/SRX8245464.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8245464/SRX8245464.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8245464/SRX8245464.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8245464/SRX8245464.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 20:44:22: #1 read tag files... INFO @ Sat, 15 Jan 2022 20:44:22: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 20:44:25: 6000000 INFO @ Sat, 15 Jan 2022 20:44:28: 1000000 INFO @ Sat, 15 Jan 2022 20:44:32: 7000000 INFO @ Sat, 15 Jan 2022 20:44:36: 2000000 INFO @ Sat, 15 Jan 2022 20:44:39: 8000000 INFO @ Sat, 15 Jan 2022 20:44:42: 3000000 INFO @ Sat, 15 Jan 2022 20:44:46: 9000000 INFO @ Sat, 15 Jan 2022 20:44:49: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 20:44:52: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8245464/SRX8245464.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8245464/SRX8245464.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8245464/SRX8245464.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8245464/SRX8245464.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 20:44:52: #1 read tag files... INFO @ Sat, 15 Jan 2022 20:44:52: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 20:44:53: 10000000 INFO @ Sat, 15 Jan 2022 20:44:56: 5000000 INFO @ Sat, 15 Jan 2022 20:44:59: 1000000 INFO @ Sat, 15 Jan 2022 20:45:00: 11000000 INFO @ Sat, 15 Jan 2022 20:45:03: 6000000 INFO @ Sat, 15 Jan 2022 20:45:06: 2000000 INFO @ Sat, 15 Jan 2022 20:45:07: 12000000 INFO @ Sat, 15 Jan 2022 20:45:09: 7000000 INFO @ Sat, 15 Jan 2022 20:45:13: 13000000 INFO @ Sat, 15 Jan 2022 20:45:14: 3000000 INFO @ Sat, 15 Jan 2022 20:45:16: 8000000 INFO @ Sat, 15 Jan 2022 20:45:20: 14000000 INFO @ Sat, 15 Jan 2022 20:45:21: 4000000 INFO @ Sat, 15 Jan 2022 20:45:23: 9000000 INFO @ Sat, 15 Jan 2022 20:45:27: 15000000 INFO @ Sat, 15 Jan 2022 20:45:27: 5000000 INFO @ Sat, 15 Jan 2022 20:45:30: 10000000 INFO @ Sat, 15 Jan 2022 20:45:34: 6000000 INFO @ Sat, 15 Jan 2022 20:45:34: 16000000 INFO @ Sat, 15 Jan 2022 20:45:37: 11000000 INFO @ Sat, 15 Jan 2022 20:45:41: 7000000 INFO @ Sat, 15 Jan 2022 20:45:41: 17000000 INFO @ Sat, 15 Jan 2022 20:45:44: 12000000 INFO @ Sat, 15 Jan 2022 20:45:47: 8000000 INFO @ Sat, 15 Jan 2022 20:45:48: 18000000 INFO @ Sat, 15 Jan 2022 20:45:50: 13000000 INFO @ Sat, 15 Jan 2022 20:45:54: 9000000 INFO @ Sat, 15 Jan 2022 20:45:56: 19000000 INFO @ Sat, 15 Jan 2022 20:45:57: 14000000 INFO @ Sat, 15 Jan 2022 20:46:00: 10000000 INFO @ Sat, 15 Jan 2022 20:46:03: 20000000 INFO @ Sat, 15 Jan 2022 20:46:04: 15000000 INFO @ Sat, 15 Jan 2022 20:46:07: 11000000 INFO @ Sat, 15 Jan 2022 20:46:10: 21000000 INFO @ Sat, 15 Jan 2022 20:46:11: 16000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 20:46:14: 12000000 INFO @ Sat, 15 Jan 2022 20:46:17: 17000000 INFO @ Sat, 15 Jan 2022 20:46:18: 22000000 INFO @ Sat, 15 Jan 2022 20:46:21: 13000000 INFO @ Sat, 15 Jan 2022 20:46:24: 18000000 INFO @ Sat, 15 Jan 2022 20:46:25: 23000000 BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 20:46:28: 14000000 INFO @ Sat, 15 Jan 2022 20:46:31: 19000000 INFO @ Sat, 15 Jan 2022 20:46:32: 24000000 INFO @ Sat, 15 Jan 2022 20:46:34: 15000000 INFO @ Sat, 15 Jan 2022 20:46:38: 20000000 INFO @ Sat, 15 Jan 2022 20:46:40: 25000000 INFO @ Sat, 15 Jan 2022 20:46:41: 16000000 INFO @ Sat, 15 Jan 2022 20:46:45: 21000000 INFO @ Sat, 15 Jan 2022 20:46:47: 26000000 INFO @ Sat, 15 Jan 2022 20:46:48: #1 tag size is determined as 75 bps INFO @ Sat, 15 Jan 2022 20:46:48: #1 tag size = 75 INFO @ Sat, 15 Jan 2022 20:46:48: #1 total tags in treatment: 12212759 INFO @ Sat, 15 Jan 2022 20:46:48: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 20:46:48: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 20:46:48: 17000000 INFO @ Sat, 15 Jan 2022 20:46:48: #1 tags after filtering in treatment: 6352212 INFO @ Sat, 15 Jan 2022 20:46:48: #1 Redundant rate of treatment: 0.48 INFO @ Sat, 15 Jan 2022 20:46:48: #1 finished! INFO @ Sat, 15 Jan 2022 20:46:48: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 20:46:48: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 20:46:48: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 20:46:48: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 20:46:48: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8245464/SRX8245464.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245464/SRX8245464.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245464/SRX8245464.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245464/SRX8245464.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 20:46:51: 22000000 INFO @ Sat, 15 Jan 2022 20:46:55: 18000000 INFO @ Sat, 15 Jan 2022 20:46:58: 23000000 INFO @ Sat, 15 Jan 2022 20:47:01: 19000000 INFO @ Sat, 15 Jan 2022 20:47:05: 24000000 INFO @ Sat, 15 Jan 2022 20:47:08: 20000000 INFO @ Sat, 15 Jan 2022 20:47:11: 25000000 INFO @ Sat, 15 Jan 2022 20:47:14: 21000000 INFO @ Sat, 15 Jan 2022 20:47:18: 26000000 INFO @ Sat, 15 Jan 2022 20:47:18: #1 tag size is determined as 75 bps INFO @ Sat, 15 Jan 2022 20:47:18: #1 tag size = 75 INFO @ Sat, 15 Jan 2022 20:47:18: #1 total tags in treatment: 12212759 INFO @ Sat, 15 Jan 2022 20:47:18: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 20:47:18: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 20:47:19: #1 tags after filtering in treatment: 6352212 INFO @ Sat, 15 Jan 2022 20:47:19: #1 Redundant rate of treatment: 0.48 INFO @ Sat, 15 Jan 2022 20:47:19: #1 finished! INFO @ Sat, 15 Jan 2022 20:47:19: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 20:47:19: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 20:47:19: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 20:47:19: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 20:47:19: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8245464/SRX8245464.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 30 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245464/SRX8245464.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245464/SRX8245464.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245464/SRX8245464.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 20:47:21: 22000000 INFO @ Sat, 15 Jan 2022 20:47:27: 23000000 INFO @ Sat, 15 Jan 2022 20:47:33: 24000000 INFO @ Sat, 15 Jan 2022 20:47:39: 25000000 INFO @ Sat, 15 Jan 2022 20:47:45: 26000000 INFO @ Sat, 15 Jan 2022 20:47:46: #1 tag size is determined as 75 bps INFO @ Sat, 15 Jan 2022 20:47:46: #1 tag size = 75 INFO @ Sat, 15 Jan 2022 20:47:46: #1 total tags in treatment: 12212759 INFO @ Sat, 15 Jan 2022 20:47:46: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 20:47:46: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 20:47:46: #1 tags after filtering in treatment: 6352212 INFO @ Sat, 15 Jan 2022 20:47:46: #1 Redundant rate of treatment: 0.48 INFO @ Sat, 15 Jan 2022 20:47:46: #1 finished! INFO @ Sat, 15 Jan 2022 20:47:46: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 20:47:46: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 20:47:46: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 20:47:46: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 20:47:46: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8245464/SRX8245464.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245464/SRX8245464.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245464/SRX8245464.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245464/SRX8245464.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling