Job ID = 14521040 SRX = SRX8245463 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 15969771 spots for SRR11684674/SRR11684674.sra Written 15969771 spots for SRR11684674/SRR11684674.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:16:44 15969771 reads; of these: 15969771 (100.00%) were paired; of these: 2750181 (17.22%) aligned concordantly 0 times 12041290 (75.40%) aligned concordantly exactly 1 time 1178300 (7.38%) aligned concordantly >1 times ---- 2750181 pairs aligned concordantly 0 times; of these: 35453 (1.29%) aligned discordantly 1 time ---- 2714728 pairs aligned 0 times concordantly or discordantly; of these: 5429456 mates make up the pairs; of these: 3578054 (65.90%) aligned 0 times 1651392 (30.42%) aligned exactly 1 time 200010 (3.68%) aligned >1 times 88.80% overall alignment rate Time searching: 00:16:44 Overall time: 00:16:44 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 12 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 1806641 / 13253432 = 0.1363 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 20:47:43: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8245463/SRX8245463.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8245463/SRX8245463.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8245463/SRX8245463.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8245463/SRX8245463.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 20:47:43: #1 read tag files... INFO @ Sat, 15 Jan 2022 20:47:43: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 20:47:48: 1000000 INFO @ Sat, 15 Jan 2022 20:47:54: 2000000 INFO @ Sat, 15 Jan 2022 20:47:59: 3000000 INFO @ Sat, 15 Jan 2022 20:48:05: 4000000 INFO @ Sat, 15 Jan 2022 20:48:10: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 20:48:13: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8245463/SRX8245463.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8245463/SRX8245463.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8245463/SRX8245463.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8245463/SRX8245463.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 20:48:13: #1 read tag files... INFO @ Sat, 15 Jan 2022 20:48:13: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 20:48:15: 6000000 INFO @ Sat, 15 Jan 2022 20:48:19: 1000000 INFO @ Sat, 15 Jan 2022 20:48:20: 7000000 INFO @ Sat, 15 Jan 2022 20:48:24: 2000000 INFO @ Sat, 15 Jan 2022 20:48:25: 8000000 INFO @ Sat, 15 Jan 2022 20:48:29: 3000000 INFO @ Sat, 15 Jan 2022 20:48:30: 9000000 INFO @ Sat, 15 Jan 2022 20:48:35: 4000000 INFO @ Sat, 15 Jan 2022 20:48:35: 10000000 INFO @ Sat, 15 Jan 2022 20:48:40: 5000000 INFO @ Sat, 15 Jan 2022 20:48:41: 11000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 20:48:43: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8245463/SRX8245463.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8245463/SRX8245463.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8245463/SRX8245463.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8245463/SRX8245463.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 20:48:43: #1 read tag files... INFO @ Sat, 15 Jan 2022 20:48:43: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 20:48:45: 6000000 INFO @ Sat, 15 Jan 2022 20:48:46: 12000000 INFO @ Sat, 15 Jan 2022 20:48:49: 1000000 INFO @ Sat, 15 Jan 2022 20:48:50: 7000000 INFO @ Sat, 15 Jan 2022 20:48:51: 13000000 INFO @ Sat, 15 Jan 2022 20:48:55: 2000000 INFO @ Sat, 15 Jan 2022 20:48:56: 8000000 INFO @ Sat, 15 Jan 2022 20:48:56: 14000000 INFO @ Sat, 15 Jan 2022 20:49:01: 3000000 INFO @ Sat, 15 Jan 2022 20:49:01: 9000000 INFO @ Sat, 15 Jan 2022 20:49:02: 15000000 INFO @ Sat, 15 Jan 2022 20:49:06: 4000000 INFO @ Sat, 15 Jan 2022 20:49:07: 10000000 INFO @ Sat, 15 Jan 2022 20:49:07: 16000000 INFO @ Sat, 15 Jan 2022 20:49:12: 5000000 INFO @ Sat, 15 Jan 2022 20:49:12: 11000000 INFO @ Sat, 15 Jan 2022 20:49:12: 17000000 INFO @ Sat, 15 Jan 2022 20:49:18: 6000000 INFO @ Sat, 15 Jan 2022 20:49:18: 12000000 INFO @ Sat, 15 Jan 2022 20:49:18: 18000000 INFO @ Sat, 15 Jan 2022 20:49:23: 7000000 INFO @ Sat, 15 Jan 2022 20:49:23: 19000000 INFO @ Sat, 15 Jan 2022 20:49:24: 13000000 INFO @ Sat, 15 Jan 2022 20:49:29: 20000000 INFO @ Sat, 15 Jan 2022 20:49:29: 8000000 INFO @ Sat, 15 Jan 2022 20:49:29: 14000000 INFO @ Sat, 15 Jan 2022 20:49:34: 21000000 INFO @ Sat, 15 Jan 2022 20:49:34: 9000000 INFO @ Sat, 15 Jan 2022 20:49:35: 15000000 INFO @ Sat, 15 Jan 2022 20:49:39: 22000000 INFO @ Sat, 15 Jan 2022 20:49:40: 10000000 INFO @ Sat, 15 Jan 2022 20:49:41: 16000000 INFO @ Sat, 15 Jan 2022 20:49:45: 23000000 INFO @ Sat, 15 Jan 2022 20:49:46: 11000000 INFO @ Sat, 15 Jan 2022 20:49:47: 17000000 INFO @ Sat, 15 Jan 2022 20:49:50: 24000000 INFO @ Sat, 15 Jan 2022 20:49:51: 12000000 INFO @ Sat, 15 Jan 2022 20:49:53: 18000000 INFO @ Sat, 15 Jan 2022 20:49:54: #1 tag size is determined as 75 bps INFO @ Sat, 15 Jan 2022 20:49:54: #1 tag size = 75 INFO @ Sat, 15 Jan 2022 20:49:54: #1 total tags in treatment: 11415470 INFO @ Sat, 15 Jan 2022 20:49:54: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 20:49:54: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 20:49:55: #1 tags after filtering in treatment: 5519046 INFO @ Sat, 15 Jan 2022 20:49:55: #1 Redundant rate of treatment: 0.52 INFO @ Sat, 15 Jan 2022 20:49:55: #1 finished! INFO @ Sat, 15 Jan 2022 20:49:55: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 20:49:55: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 20:49:55: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 20:49:55: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 20:49:55: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8245463/SRX8245463.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245463/SRX8245463.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245463/SRX8245463.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245463/SRX8245463.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 20:49:57: 13000000 INFO @ Sat, 15 Jan 2022 20:49:59: 19000000 INFO @ Sat, 15 Jan 2022 20:50:03: 14000000 INFO @ Sat, 15 Jan 2022 20:50:04: 20000000 INFO @ Sat, 15 Jan 2022 20:50:09: 15000000 INFO @ Sat, 15 Jan 2022 20:50:10: 21000000 INFO @ Sat, 15 Jan 2022 20:50:15: 16000000 INFO @ Sat, 15 Jan 2022 20:50:16: 22000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 20:50:21: 17000000 INFO @ Sat, 15 Jan 2022 20:50:22: 23000000 INFO @ Sat, 15 Jan 2022 20:50:26: 18000000 INFO @ Sat, 15 Jan 2022 20:50:28: 24000000 INFO @ Sat, 15 Jan 2022 20:50:32: 19000000 INFO @ Sat, 15 Jan 2022 20:50:32: #1 tag size is determined as 75 bps INFO @ Sat, 15 Jan 2022 20:50:32: #1 tag size = 75 INFO @ Sat, 15 Jan 2022 20:50:32: #1 total tags in treatment: 11415470 INFO @ Sat, 15 Jan 2022 20:50:32: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 20:50:32: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 20:50:32: #1 tags after filtering in treatment: 5519046 INFO @ Sat, 15 Jan 2022 20:50:32: #1 Redundant rate of treatment: 0.52 INFO @ Sat, 15 Jan 2022 20:50:32: #1 finished! INFO @ Sat, 15 Jan 2022 20:50:32: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 20:50:32: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 20:50:33: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 20:50:33: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 20:50:33: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8245463/SRX8245463.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245463/SRX8245463.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245463/SRX8245463.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245463/SRX8245463.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 20:50:37: 20000000 INFO @ Sat, 15 Jan 2022 20:50:43: 21000000 INFO @ Sat, 15 Jan 2022 20:50:48: 22000000 INFO @ Sat, 15 Jan 2022 20:50:53: 23000000 INFO @ Sat, 15 Jan 2022 20:50:59: 24000000 INFO @ Sat, 15 Jan 2022 20:51:03: #1 tag size is determined as 75 bps INFO @ Sat, 15 Jan 2022 20:51:03: #1 tag size = 75 INFO @ Sat, 15 Jan 2022 20:51:03: #1 total tags in treatment: 11415470 INFO @ Sat, 15 Jan 2022 20:51:03: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 20:51:03: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 20:51:03: #1 tags after filtering in treatment: 5519046 INFO @ Sat, 15 Jan 2022 20:51:03: #1 Redundant rate of treatment: 0.52 INFO @ Sat, 15 Jan 2022 20:51:03: #1 finished! INFO @ Sat, 15 Jan 2022 20:51:03: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 20:51:03: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 20:51:03: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 20:51:03: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 20:51:03: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8245463/SRX8245463.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245463/SRX8245463.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245463/SRX8245463.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245463/SRX8245463.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling