Job ID = 14521039 SRX = SRX8245462 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 16748649 spots for SRR11684673/SRR11684673.sra Written 16748649 spots for SRR11684673/SRR11684673.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:13:11 16748649 reads; of these: 16748649 (100.00%) were paired; of these: 2981576 (17.80%) aligned concordantly 0 times 12463130 (74.41%) aligned concordantly exactly 1 time 1303943 (7.79%) aligned concordantly >1 times ---- 2981576 pairs aligned concordantly 0 times; of these: 34844 (1.17%) aligned discordantly 1 time ---- 2946732 pairs aligned 0 times concordantly or discordantly; of these: 5893464 mates make up the pairs; of these: 3906371 (66.28%) aligned 0 times 1761696 (29.89%) aligned exactly 1 time 225397 (3.82%) aligned >1 times 88.34% overall alignment rate Time searching: 00:13:11 Overall time: 00:13:11 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 16 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 1740304 / 13800063 = 0.1261 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 20:45:38: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8245462/SRX8245462.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8245462/SRX8245462.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8245462/SRX8245462.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8245462/SRX8245462.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 20:45:38: #1 read tag files... INFO @ Sat, 15 Jan 2022 20:45:38: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 20:45:44: 1000000 INFO @ Sat, 15 Jan 2022 20:45:49: 2000000 INFO @ Sat, 15 Jan 2022 20:45:55: 3000000 INFO @ Sat, 15 Jan 2022 20:46:01: 4000000 INFO @ Sat, 15 Jan 2022 20:46:06: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 20:46:08: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8245462/SRX8245462.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8245462/SRX8245462.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8245462/SRX8245462.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8245462/SRX8245462.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 20:46:08: #1 read tag files... INFO @ Sat, 15 Jan 2022 20:46:08: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 20:46:12: 6000000 INFO @ Sat, 15 Jan 2022 20:46:14: 1000000 INFO @ Sat, 15 Jan 2022 20:46:17: 7000000 INFO @ Sat, 15 Jan 2022 20:46:20: 2000000 INFO @ Sat, 15 Jan 2022 20:46:22: 8000000 INFO @ Sat, 15 Jan 2022 20:46:25: 3000000 INFO @ Sat, 15 Jan 2022 20:46:27: 9000000 INFO @ Sat, 15 Jan 2022 20:46:31: 4000000 INFO @ Sat, 15 Jan 2022 20:46:33: 10000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 20:46:37: 5000000 INFO @ Sat, 15 Jan 2022 20:46:38: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8245462/SRX8245462.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8245462/SRX8245462.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8245462/SRX8245462.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8245462/SRX8245462.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 20:46:38: #1 read tag files... INFO @ Sat, 15 Jan 2022 20:46:38: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 20:46:38: 11000000 INFO @ Sat, 15 Jan 2022 20:46:43: 6000000 INFO @ Sat, 15 Jan 2022 20:46:44: 1000000 INFO @ Sat, 15 Jan 2022 20:46:44: 12000000 INFO @ Sat, 15 Jan 2022 20:46:48: 7000000 INFO @ Sat, 15 Jan 2022 20:46:50: 2000000 INFO @ Sat, 15 Jan 2022 20:46:50: 13000000 INFO @ Sat, 15 Jan 2022 20:46:53: 8000000 INFO @ Sat, 15 Jan 2022 20:46:56: 14000000 INFO @ Sat, 15 Jan 2022 20:46:56: 3000000 INFO @ Sat, 15 Jan 2022 20:46:58: 9000000 INFO @ Sat, 15 Jan 2022 20:47:02: 4000000 INFO @ Sat, 15 Jan 2022 20:47:02: 15000000 INFO @ Sat, 15 Jan 2022 20:47:04: 10000000 INFO @ Sat, 15 Jan 2022 20:47:07: 5000000 INFO @ Sat, 15 Jan 2022 20:47:08: 16000000 INFO @ Sat, 15 Jan 2022 20:47:10: 11000000 INFO @ Sat, 15 Jan 2022 20:47:13: 6000000 INFO @ Sat, 15 Jan 2022 20:47:14: 17000000 INFO @ Sat, 15 Jan 2022 20:47:16: 12000000 INFO @ Sat, 15 Jan 2022 20:47:18: 7000000 INFO @ Sat, 15 Jan 2022 20:47:20: 18000000 INFO @ Sat, 15 Jan 2022 20:47:22: 13000000 INFO @ Sat, 15 Jan 2022 20:47:23: 8000000 INFO @ Sat, 15 Jan 2022 20:47:26: 19000000 INFO @ Sat, 15 Jan 2022 20:47:28: 14000000 INFO @ Sat, 15 Jan 2022 20:47:28: 9000000 INFO @ Sat, 15 Jan 2022 20:47:32: 20000000 INFO @ Sat, 15 Jan 2022 20:47:34: 15000000 INFO @ Sat, 15 Jan 2022 20:47:34: 10000000 INFO @ Sat, 15 Jan 2022 20:47:38: 21000000 INFO @ Sat, 15 Jan 2022 20:47:40: 11000000 INFO @ Sat, 15 Jan 2022 20:47:40: 16000000 INFO @ Sat, 15 Jan 2022 20:47:44: 22000000 INFO @ Sat, 15 Jan 2022 20:47:46: 12000000 INFO @ Sat, 15 Jan 2022 20:47:46: 17000000 INFO @ Sat, 15 Jan 2022 20:47:51: 23000000 INFO @ Sat, 15 Jan 2022 20:47:51: 13000000 INFO @ Sat, 15 Jan 2022 20:47:52: 18000000 INFO @ Sat, 15 Jan 2022 20:47:57: 24000000 INFO @ Sat, 15 Jan 2022 20:47:57: 14000000 INFO @ Sat, 15 Jan 2022 20:47:58: 19000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 20:48:03: 15000000 INFO @ Sat, 15 Jan 2022 20:48:03: 25000000 INFO @ Sat, 15 Jan 2022 20:48:04: 20000000 INFO @ Sat, 15 Jan 2022 20:48:09: 26000000 INFO @ Sat, 15 Jan 2022 20:48:09: 16000000 INFO @ Sat, 15 Jan 2022 20:48:09: #1 tag size is determined as 75 bps INFO @ Sat, 15 Jan 2022 20:48:09: #1 tag size = 75 INFO @ Sat, 15 Jan 2022 20:48:09: #1 total tags in treatment: 12029305 INFO @ Sat, 15 Jan 2022 20:48:09: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 20:48:09: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 20:48:10: #1 tags after filtering in treatment: 5863054 INFO @ Sat, 15 Jan 2022 20:48:10: #1 Redundant rate of treatment: 0.51 INFO @ Sat, 15 Jan 2022 20:48:10: #1 finished! INFO @ Sat, 15 Jan 2022 20:48:10: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 20:48:10: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 20:48:10: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 20:48:10: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 20:48:10: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8245462/SRX8245462.05_peaks.narrowPeak: No such file or directory INFO @ Sat, 15 Jan 2022 20:48:10: 21000000 pass1 - making usageList (0 chroms): 951 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245462/SRX8245462.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245462/SRX8245462.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245462/SRX8245462.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 20:48:15: 17000000 INFO @ Sat, 15 Jan 2022 20:48:16: 22000000 INFO @ Sat, 15 Jan 2022 20:48:21: 18000000 INFO @ Sat, 15 Jan 2022 20:48:22: 23000000 INFO @ Sat, 15 Jan 2022 20:48:27: 19000000 INFO @ Sat, 15 Jan 2022 20:48:28: 24000000 INFO @ Sat, 15 Jan 2022 20:48:32: 20000000 INFO @ Sat, 15 Jan 2022 20:48:35: 25000000 INFO @ Sat, 15 Jan 2022 20:48:38: 21000000 INFO @ Sat, 15 Jan 2022 20:48:40: 26000000 INFO @ Sat, 15 Jan 2022 20:48:41: #1 tag size is determined as 75 bps INFO @ Sat, 15 Jan 2022 20:48:41: #1 tag size = 75 INFO @ Sat, 15 Jan 2022 20:48:41: #1 total tags in treatment: 12029305 INFO @ Sat, 15 Jan 2022 20:48:41: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 20:48:41: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 20:48:41: #1 tags after filtering in treatment: 5863054 INFO @ Sat, 15 Jan 2022 20:48:41: #1 Redundant rate of treatment: 0.51 INFO @ Sat, 15 Jan 2022 20:48:41: #1 finished! INFO @ Sat, 15 Jan 2022 20:48:41: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 20:48:41: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 20:48:41: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 20:48:41: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 20:48:41: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8245462/SRX8245462.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245462/SRX8245462.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245462/SRX8245462.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245462/SRX8245462.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 20:48:44: 22000000 INFO @ Sat, 15 Jan 2022 20:48:50: 23000000 INFO @ Sat, 15 Jan 2022 20:48:56: 24000000 INFO @ Sat, 15 Jan 2022 20:49:02: 25000000 INFO @ Sat, 15 Jan 2022 20:49:07: 26000000 INFO @ Sat, 15 Jan 2022 20:49:08: #1 tag size is determined as 75 bps INFO @ Sat, 15 Jan 2022 20:49:08: #1 tag size = 75 INFO @ Sat, 15 Jan 2022 20:49:08: #1 total tags in treatment: 12029305 INFO @ Sat, 15 Jan 2022 20:49:08: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 20:49:08: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 20:49:08: #1 tags after filtering in treatment: 5863054 INFO @ Sat, 15 Jan 2022 20:49:08: #1 Redundant rate of treatment: 0.51 INFO @ Sat, 15 Jan 2022 20:49:08: #1 finished! INFO @ Sat, 15 Jan 2022 20:49:08: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 20:49:08: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 20:49:08: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 20:49:08: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 20:49:08: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8245462/SRX8245462.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245462/SRX8245462.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245462/SRX8245462.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245462/SRX8245462.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling