Job ID = 14520980 SRX = SRX8245458 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 16044254 spots for SRR11684669/SRR11684669.sra Written 16044254 spots for SRR11684669/SRR11684669.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:19:40 16044254 reads; of these: 16044254 (100.00%) were paired; of these: 2669418 (16.64%) aligned concordantly 0 times 12262578 (76.43%) aligned concordantly exactly 1 time 1112258 (6.93%) aligned concordantly >1 times ---- 2669418 pairs aligned concordantly 0 times; of these: 79196 (2.97%) aligned discordantly 1 time ---- 2590222 pairs aligned 0 times concordantly or discordantly; of these: 5180444 mates make up the pairs; of these: 3410622 (65.84%) aligned 0 times 1580353 (30.51%) aligned exactly 1 time 189469 (3.66%) aligned >1 times 89.37% overall alignment rate Time searching: 00:19:40 Overall time: 00:19:40 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 12 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 1964252 / 13452595 = 0.1460 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 20:46:27: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8245458/SRX8245458.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8245458/SRX8245458.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8245458/SRX8245458.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8245458/SRX8245458.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 20:46:27: #1 read tag files... INFO @ Sat, 15 Jan 2022 20:46:27: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 20:46:35: 1000000 INFO @ Sat, 15 Jan 2022 20:46:43: 2000000 INFO @ Sat, 15 Jan 2022 20:46:50: 3000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 20:46:57: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8245458/SRX8245458.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8245458/SRX8245458.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8245458/SRX8245458.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8245458/SRX8245458.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 20:46:57: #1 read tag files... INFO @ Sat, 15 Jan 2022 20:46:57: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 20:46:58: 4000000 INFO @ Sat, 15 Jan 2022 20:47:04: 1000000 INFO @ Sat, 15 Jan 2022 20:47:06: 5000000 INFO @ Sat, 15 Jan 2022 20:47:11: 2000000 INFO @ Sat, 15 Jan 2022 20:47:14: 6000000 INFO @ Sat, 15 Jan 2022 20:47:18: 3000000 INFO @ Sat, 15 Jan 2022 20:47:21: 7000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container BedGraph に変換中... INFO @ Sat, 15 Jan 2022 20:47:25: 4000000 INFO @ Sat, 15 Jan 2022 20:47:27: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8245458/SRX8245458.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8245458/SRX8245458.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8245458/SRX8245458.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8245458/SRX8245458.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 20:47:27: #1 read tag files... INFO @ Sat, 15 Jan 2022 20:47:27: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 20:47:29: 8000000 INFO @ Sat, 15 Jan 2022 20:47:33: 5000000 INFO @ Sat, 15 Jan 2022 20:47:35: 1000000 INFO @ Sat, 15 Jan 2022 20:47:37: 9000000 INFO @ Sat, 15 Jan 2022 20:47:40: 6000000 INFO @ Sat, 15 Jan 2022 20:47:44: 2000000 INFO @ Sat, 15 Jan 2022 20:47:45: 10000000 INFO @ Sat, 15 Jan 2022 20:47:47: 7000000 INFO @ Sat, 15 Jan 2022 20:47:52: 3000000 INFO @ Sat, 15 Jan 2022 20:47:53: 11000000 INFO @ Sat, 15 Jan 2022 20:47:54: 8000000 INFO @ Sat, 15 Jan 2022 20:48:00: 4000000 INFO @ Sat, 15 Jan 2022 20:48:01: 9000000 INFO @ Sat, 15 Jan 2022 20:48:01: 12000000 INFO @ Sat, 15 Jan 2022 20:48:08: 10000000 INFO @ Sat, 15 Jan 2022 20:48:09: 5000000 INFO @ Sat, 15 Jan 2022 20:48:09: 13000000 INFO @ Sat, 15 Jan 2022 20:48:16: 11000000 INFO @ Sat, 15 Jan 2022 20:48:17: 6000000 INFO @ Sat, 15 Jan 2022 20:48:17: 14000000 INFO @ Sat, 15 Jan 2022 20:48:23: 12000000 INFO @ Sat, 15 Jan 2022 20:48:25: 7000000 INFO @ Sat, 15 Jan 2022 20:48:27: 15000000 INFO @ Sat, 15 Jan 2022 20:48:31: 13000000 INFO @ Sat, 15 Jan 2022 20:48:34: 8000000 INFO @ Sat, 15 Jan 2022 20:48:36: 16000000 INFO @ Sat, 15 Jan 2022 20:48:38: 14000000 INFO @ Sat, 15 Jan 2022 20:48:41: 9000000 INFO @ Sat, 15 Jan 2022 20:48:44: 17000000 INFO @ Sat, 15 Jan 2022 20:48:45: 15000000 INFO @ Sat, 15 Jan 2022 20:48:50: 10000000 INFO @ Sat, 15 Jan 2022 20:48:53: 16000000 INFO @ Sat, 15 Jan 2022 20:48:53: 18000000 INFO @ Sat, 15 Jan 2022 20:48:59: 11000000 INFO @ Sat, 15 Jan 2022 20:49:02: 17000000 INFO @ Sat, 15 Jan 2022 20:49:02: 19000000 INFO @ Sat, 15 Jan 2022 20:49:07: 12000000 INFO @ Sat, 15 Jan 2022 20:49:10: 18000000 INFO @ Sat, 15 Jan 2022 20:49:11: 20000000 INFO @ Sat, 15 Jan 2022 20:49:15: 13000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 20:49:18: 19000000 INFO @ Sat, 15 Jan 2022 20:49:19: 21000000 INFO @ Sat, 15 Jan 2022 20:49:23: 14000000 INFO @ Sat, 15 Jan 2022 20:49:26: 20000000 INFO @ Sat, 15 Jan 2022 20:49:27: 22000000 INFO @ Sat, 15 Jan 2022 20:49:31: 15000000 BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 20:49:34: 21000000 INFO @ Sat, 15 Jan 2022 20:49:35: 23000000 INFO @ Sat, 15 Jan 2022 20:49:40: 16000000 INFO @ Sat, 15 Jan 2022 20:49:42: 22000000 INFO @ Sat, 15 Jan 2022 20:49:45: 24000000 INFO @ Sat, 15 Jan 2022 20:49:49: 17000000 INFO @ Sat, 15 Jan 2022 20:49:51: 23000000 INFO @ Sat, 15 Jan 2022 20:49:52: #1 tag size is determined as 75 bps INFO @ Sat, 15 Jan 2022 20:49:52: #1 tag size = 75 INFO @ Sat, 15 Jan 2022 20:49:52: #1 total tags in treatment: 11415163 INFO @ Sat, 15 Jan 2022 20:49:52: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 20:49:52: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 20:49:52: #1 tags after filtering in treatment: 5034522 INFO @ Sat, 15 Jan 2022 20:49:52: #1 Redundant rate of treatment: 0.56 INFO @ Sat, 15 Jan 2022 20:49:52: #1 finished! INFO @ Sat, 15 Jan 2022 20:49:52: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 20:49:52: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 20:49:53: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 20:49:53: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 20:49:53: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8245458/SRX8245458.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245458/SRX8245458.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245458/SRX8245458.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245458/SRX8245458.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 20:49:58: 18000000 INFO @ Sat, 15 Jan 2022 20:49:59: 24000000 INFO @ Sat, 15 Jan 2022 20:50:05: #1 tag size is determined as 75 bps INFO @ Sat, 15 Jan 2022 20:50:05: #1 tag size = 75 INFO @ Sat, 15 Jan 2022 20:50:05: #1 total tags in treatment: 11415163 INFO @ Sat, 15 Jan 2022 20:50:05: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 20:50:05: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 20:50:06: 19000000 INFO @ Sat, 15 Jan 2022 20:50:06: #1 tags after filtering in treatment: 5034522 INFO @ Sat, 15 Jan 2022 20:50:06: #1 Redundant rate of treatment: 0.56 INFO @ Sat, 15 Jan 2022 20:50:06: #1 finished! INFO @ Sat, 15 Jan 2022 20:50:06: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 20:50:06: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 20:50:06: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 20:50:06: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 20:50:06: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8245458/SRX8245458.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245458/SRX8245458.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245458/SRX8245458.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245458/SRX8245458.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 20:50:13: 20000000 INFO @ Sat, 15 Jan 2022 20:50:21: 21000000 INFO @ Sat, 15 Jan 2022 20:50:29: 22000000 INFO @ Sat, 15 Jan 2022 20:50:37: 23000000 INFO @ Sat, 15 Jan 2022 20:50:45: 24000000 INFO @ Sat, 15 Jan 2022 20:50:53: #1 tag size is determined as 75 bps INFO @ Sat, 15 Jan 2022 20:50:53: #1 tag size = 75 INFO @ Sat, 15 Jan 2022 20:50:53: #1 total tags in treatment: 11415163 INFO @ Sat, 15 Jan 2022 20:50:53: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 20:50:53: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 20:50:54: #1 tags after filtering in treatment: 5034522 INFO @ Sat, 15 Jan 2022 20:50:54: #1 Redundant rate of treatment: 0.56 INFO @ Sat, 15 Jan 2022 20:50:54: #1 finished! INFO @ Sat, 15 Jan 2022 20:50:54: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 20:50:54: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 20:50:54: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 20:50:54: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 20:50:54: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8245458/SRX8245458.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245458/SRX8245458.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245458/SRX8245458.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245458/SRX8245458.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling