Job ID = 14520977 SRX = SRX8245455 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 21540462 spots for SRR11684666/SRR11684666.sra Written 21540462 spots for SRR11684666/SRR11684666.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:19:49 21540462 reads; of these: 21540462 (100.00%) were paired; of these: 11242861 (52.19%) aligned concordantly 0 times 8391028 (38.95%) aligned concordantly exactly 1 time 1906573 (8.85%) aligned concordantly >1 times ---- 11242861 pairs aligned concordantly 0 times; of these: 51971 (0.46%) aligned discordantly 1 time ---- 11190890 pairs aligned 0 times concordantly or discordantly; of these: 22381780 mates make up the pairs; of these: 12098520 (54.06%) aligned 0 times 8311800 (37.14%) aligned exactly 1 time 1971460 (8.81%) aligned >1 times 71.92% overall alignment rate Time searching: 00:19:49 Overall time: 00:19:49 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 16 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 484929 / 10347942 = 0.0469 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 20:44:52: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8245455/SRX8245455.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8245455/SRX8245455.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8245455/SRX8245455.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8245455/SRX8245455.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 20:44:52: #1 read tag files... INFO @ Sat, 15 Jan 2022 20:44:52: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 20:44:57: 1000000 INFO @ Sat, 15 Jan 2022 20:45:02: 2000000 INFO @ Sat, 15 Jan 2022 20:45:07: 3000000 INFO @ Sat, 15 Jan 2022 20:45:12: 4000000 INFO @ Sat, 15 Jan 2022 20:45:17: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 20:45:22: 6000000 INFO @ Sat, 15 Jan 2022 20:45:22: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8245455/SRX8245455.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8245455/SRX8245455.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8245455/SRX8245455.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8245455/SRX8245455.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 20:45:22: #1 read tag files... INFO @ Sat, 15 Jan 2022 20:45:22: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 20:45:28: 7000000 INFO @ Sat, 15 Jan 2022 20:45:28: 1000000 INFO @ Sat, 15 Jan 2022 20:45:34: 8000000 INFO @ Sat, 15 Jan 2022 20:45:34: 2000000 INFO @ Sat, 15 Jan 2022 20:45:39: 9000000 INFO @ Sat, 15 Jan 2022 20:45:40: 3000000 INFO @ Sat, 15 Jan 2022 20:45:45: 10000000 INFO @ Sat, 15 Jan 2022 20:45:46: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 20:45:51: 11000000 INFO @ Sat, 15 Jan 2022 20:45:52: 5000000 INFO @ Sat, 15 Jan 2022 20:45:52: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8245455/SRX8245455.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8245455/SRX8245455.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8245455/SRX8245455.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8245455/SRX8245455.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 20:45:52: #1 read tag files... INFO @ Sat, 15 Jan 2022 20:45:52: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 20:45:57: 12000000 INFO @ Sat, 15 Jan 2022 20:45:58: 6000000 INFO @ Sat, 15 Jan 2022 20:45:59: 1000000 INFO @ Sat, 15 Jan 2022 20:46:03: 13000000 INFO @ Sat, 15 Jan 2022 20:46:04: 7000000 INFO @ Sat, 15 Jan 2022 20:46:05: 2000000 INFO @ Sat, 15 Jan 2022 20:46:09: 14000000 INFO @ Sat, 15 Jan 2022 20:46:10: 8000000 INFO @ Sat, 15 Jan 2022 20:46:11: 3000000 INFO @ Sat, 15 Jan 2022 20:46:15: 15000000 INFO @ Sat, 15 Jan 2022 20:46:16: 9000000 INFO @ Sat, 15 Jan 2022 20:46:17: 4000000 INFO @ Sat, 15 Jan 2022 20:46:21: 16000000 INFO @ Sat, 15 Jan 2022 20:46:22: 10000000 INFO @ Sat, 15 Jan 2022 20:46:23: 5000000 INFO @ Sat, 15 Jan 2022 20:46:26: 17000000 INFO @ Sat, 15 Jan 2022 20:46:28: 11000000 INFO @ Sat, 15 Jan 2022 20:46:29: 6000000 INFO @ Sat, 15 Jan 2022 20:46:32: 18000000 INFO @ Sat, 15 Jan 2022 20:46:34: 12000000 INFO @ Sat, 15 Jan 2022 20:46:35: 7000000 INFO @ Sat, 15 Jan 2022 20:46:38: 19000000 INFO @ Sat, 15 Jan 2022 20:46:40: 13000000 INFO @ Sat, 15 Jan 2022 20:46:41: 8000000 INFO @ Sat, 15 Jan 2022 20:46:44: 20000000 INFO @ Sat, 15 Jan 2022 20:46:46: 14000000 INFO @ Sat, 15 Jan 2022 20:46:46: 9000000 INFO @ Sat, 15 Jan 2022 20:46:50: 21000000 INFO @ Sat, 15 Jan 2022 20:46:52: 15000000 INFO @ Sat, 15 Jan 2022 20:46:52: 10000000 INFO @ Sat, 15 Jan 2022 20:46:56: 22000000 INFO @ Sat, 15 Jan 2022 20:46:58: 16000000 INFO @ Sat, 15 Jan 2022 20:46:58: 11000000 INFO @ Sat, 15 Jan 2022 20:47:02: 23000000 INFO @ Sat, 15 Jan 2022 20:47:03: 17000000 INFO @ Sat, 15 Jan 2022 20:47:04: 12000000 INFO @ Sat, 15 Jan 2022 20:47:08: 24000000 INFO @ Sat, 15 Jan 2022 20:47:09: 18000000 INFO @ Sat, 15 Jan 2022 20:47:10: 13000000 INFO @ Sat, 15 Jan 2022 20:47:14: 25000000 INFO @ Sat, 15 Jan 2022 20:47:15: 19000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 20:47:15: 14000000 INFO @ Sat, 15 Jan 2022 20:47:19: 26000000 INFO @ Sat, 15 Jan 2022 20:47:21: 15000000 INFO @ Sat, 15 Jan 2022 20:47:22: 20000000 BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 20:47:25: 27000000 INFO @ Sat, 15 Jan 2022 20:47:27: 16000000 INFO @ Sat, 15 Jan 2022 20:47:28: 21000000 INFO @ Sat, 15 Jan 2022 20:47:31: 28000000 INFO @ Sat, 15 Jan 2022 20:47:33: 17000000 INFO @ Sat, 15 Jan 2022 20:47:34: 22000000 INFO @ Sat, 15 Jan 2022 20:47:37: 29000000 INFO @ Sat, 15 Jan 2022 20:47:39: 18000000 INFO @ Sat, 15 Jan 2022 20:47:40: 23000000 INFO @ Sat, 15 Jan 2022 20:47:43: 30000000 INFO @ Sat, 15 Jan 2022 20:47:43: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 20:47:43: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 20:47:43: #1 total tags in treatment: 9819663 INFO @ Sat, 15 Jan 2022 20:47:43: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 20:47:43: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 20:47:43: #1 tags after filtering in treatment: 3429771 INFO @ Sat, 15 Jan 2022 20:47:43: #1 Redundant rate of treatment: 0.65 INFO @ Sat, 15 Jan 2022 20:47:43: #1 finished! INFO @ Sat, 15 Jan 2022 20:47:43: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 20:47:43: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 20:47:44: #2 number of paired peaks: 9 WARNING @ Sat, 15 Jan 2022 20:47:44: Too few paired peaks (9) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 20:47:44: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8245455/SRX8245455.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245455/SRX8245455.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245455/SRX8245455.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245455/SRX8245455.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 20:47:45: 19000000 INFO @ Sat, 15 Jan 2022 20:47:46: 24000000 INFO @ Sat, 15 Jan 2022 20:47:51: 25000000 INFO @ Sat, 15 Jan 2022 20:47:51: 20000000 INFO @ Sat, 15 Jan 2022 20:47:57: 26000000 INFO @ Sat, 15 Jan 2022 20:47:57: 21000000 INFO @ Sat, 15 Jan 2022 20:48:03: 27000000 INFO @ Sat, 15 Jan 2022 20:48:03: 22000000 INFO @ Sat, 15 Jan 2022 20:48:08: 28000000 INFO @ Sat, 15 Jan 2022 20:48:09: 23000000 INFO @ Sat, 15 Jan 2022 20:48:14: 29000000 INFO @ Sat, 15 Jan 2022 20:48:14: 24000000 INFO @ Sat, 15 Jan 2022 20:48:20: 30000000 INFO @ Sat, 15 Jan 2022 20:48:20: 25000000 INFO @ Sat, 15 Jan 2022 20:48:20: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 20:48:20: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 20:48:20: #1 total tags in treatment: 9819663 INFO @ Sat, 15 Jan 2022 20:48:20: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 20:48:20: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 20:48:20: #1 tags after filtering in treatment: 3429771 INFO @ Sat, 15 Jan 2022 20:48:20: #1 Redundant rate of treatment: 0.65 INFO @ Sat, 15 Jan 2022 20:48:20: #1 finished! INFO @ Sat, 15 Jan 2022 20:48:20: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 20:48:20: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 20:48:21: #2 number of paired peaks: 9 WARNING @ Sat, 15 Jan 2022 20:48:21: Too few paired peaks (9) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 20:48:21: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8245455/SRX8245455.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245455/SRX8245455.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245455/SRX8245455.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245455/SRX8245455.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 20:48:25: 26000000 INFO @ Sat, 15 Jan 2022 20:48:31: 27000000 INFO @ Sat, 15 Jan 2022 20:48:35: 28000000 INFO @ Sat, 15 Jan 2022 20:48:40: 29000000 INFO @ Sat, 15 Jan 2022 20:48:45: 30000000 INFO @ Sat, 15 Jan 2022 20:48:46: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 20:48:46: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 20:48:46: #1 total tags in treatment: 9819663 INFO @ Sat, 15 Jan 2022 20:48:46: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 20:48:46: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 20:48:46: #1 tags after filtering in treatment: 3429771 INFO @ Sat, 15 Jan 2022 20:48:46: #1 Redundant rate of treatment: 0.65 INFO @ Sat, 15 Jan 2022 20:48:46: #1 finished! INFO @ Sat, 15 Jan 2022 20:48:46: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 20:48:46: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 20:48:46: #2 number of paired peaks: 9 WARNING @ Sat, 15 Jan 2022 20:48:46: Too few paired peaks (9) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 20:48:46: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8245455/SRX8245455.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245455/SRX8245455.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245455/SRX8245455.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245455/SRX8245455.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling