Job ID = 14520975 SRX = SRX8245453 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 12406403 spots for SRR11684664/SRR11684664.sra Written 12406403 spots for SRR11684664/SRR11684664.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:13:25 12406403 reads; of these: 12406403 (100.00%) were paired; of these: 7506537 (60.51%) aligned concordantly 0 times 3494905 (28.17%) aligned concordantly exactly 1 time 1404961 (11.32%) aligned concordantly >1 times ---- 7506537 pairs aligned concordantly 0 times; of these: 91211 (1.22%) aligned discordantly 1 time ---- 7415326 pairs aligned 0 times concordantly or discordantly; of these: 14830652 mates make up the pairs; of these: 9626713 (64.91%) aligned 0 times 3725159 (25.12%) aligned exactly 1 time 1478780 (9.97%) aligned >1 times 61.20% overall alignment rate Time searching: 00:13:25 Overall time: 00:13:25 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 8 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 456904 / 4989867 = 0.0916 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 20:32:08: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8245453/SRX8245453.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8245453/SRX8245453.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8245453/SRX8245453.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8245453/SRX8245453.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 20:32:08: #1 read tag files... INFO @ Sat, 15 Jan 2022 20:32:08: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 20:32:13: 1000000 INFO @ Sat, 15 Jan 2022 20:32:19: 2000000 INFO @ Sat, 15 Jan 2022 20:32:24: 3000000 INFO @ Sat, 15 Jan 2022 20:32:29: 4000000 INFO @ Sat, 15 Jan 2022 20:32:35: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 20:32:38: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8245453/SRX8245453.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8245453/SRX8245453.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8245453/SRX8245453.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8245453/SRX8245453.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 20:32:38: #1 read tag files... INFO @ Sat, 15 Jan 2022 20:32:38: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 20:32:40: 6000000 INFO @ Sat, 15 Jan 2022 20:32:43: 1000000 INFO @ Sat, 15 Jan 2022 20:32:46: 7000000 INFO @ Sat, 15 Jan 2022 20:32:48: 2000000 INFO @ Sat, 15 Jan 2022 20:32:52: 8000000 INFO @ Sat, 15 Jan 2022 20:32:53: 3000000 INFO @ Sat, 15 Jan 2022 20:32:57: 9000000 INFO @ Sat, 15 Jan 2022 20:32:58: 4000000 INFO @ Sat, 15 Jan 2022 20:33:02: 5000000 INFO @ Sat, 15 Jan 2022 20:33:03: 10000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 20:33:08: 6000000 INFO @ Sat, 15 Jan 2022 20:33:08: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8245453/SRX8245453.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8245453/SRX8245453.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8245453/SRX8245453.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8245453/SRX8245453.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 20:33:08: #1 read tag files... INFO @ Sat, 15 Jan 2022 20:33:08: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 20:33:09: 11000000 INFO @ Sat, 15 Jan 2022 20:33:13: 7000000 INFO @ Sat, 15 Jan 2022 20:33:13: 1000000 INFO @ Sat, 15 Jan 2022 20:33:15: 12000000 INFO @ Sat, 15 Jan 2022 20:33:17: 8000000 INFO @ Sat, 15 Jan 2022 20:33:18: 2000000 INFO @ Sat, 15 Jan 2022 20:33:20: 13000000 INFO @ Sat, 15 Jan 2022 20:33:23: 9000000 INFO @ Sat, 15 Jan 2022 20:33:23: 3000000 INFO @ Sat, 15 Jan 2022 20:33:26: 14000000 INFO @ Sat, 15 Jan 2022 20:33:27: 4000000 INFO @ Sat, 15 Jan 2022 20:33:28: 10000000 INFO @ Sat, 15 Jan 2022 20:33:28: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 20:33:28: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 20:33:28: #1 total tags in treatment: 4465942 INFO @ Sat, 15 Jan 2022 20:33:28: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 20:33:28: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 20:33:28: #1 tags after filtering in treatment: 2753437 INFO @ Sat, 15 Jan 2022 20:33:28: #1 Redundant rate of treatment: 0.38 INFO @ Sat, 15 Jan 2022 20:33:28: #1 finished! INFO @ Sat, 15 Jan 2022 20:33:28: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 20:33:28: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 20:33:28: #2 number of paired peaks: 41 WARNING @ Sat, 15 Jan 2022 20:33:28: Too few paired peaks (41) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 20:33:28: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8245453/SRX8245453.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245453/SRX8245453.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245453/SRX8245453.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245453/SRX8245453.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 20:33:32: 5000000 INFO @ Sat, 15 Jan 2022 20:33:33: 11000000 INFO @ Sat, 15 Jan 2022 20:33:37: 6000000 INFO @ Sat, 15 Jan 2022 20:33:38: 12000000 INFO @ Sat, 15 Jan 2022 20:33:42: 7000000 INFO @ Sat, 15 Jan 2022 20:33:43: 13000000 INFO @ Sat, 15 Jan 2022 20:33:47: 8000000 INFO @ Sat, 15 Jan 2022 20:33:48: 14000000 INFO @ Sat, 15 Jan 2022 20:33:49: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 20:33:49: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 20:33:49: #1 total tags in treatment: 4465942 INFO @ Sat, 15 Jan 2022 20:33:49: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 20:33:49: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 20:33:49: #1 tags after filtering in treatment: 2753437 INFO @ Sat, 15 Jan 2022 20:33:49: #1 Redundant rate of treatment: 0.38 INFO @ Sat, 15 Jan 2022 20:33:49: #1 finished! INFO @ Sat, 15 Jan 2022 20:33:49: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 20:33:49: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 20:33:50: #2 number of paired peaks: 41 WARNING @ Sat, 15 Jan 2022 20:33:50: Too few paired peaks (41) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 20:33:50: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8245453/SRX8245453.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245453/SRX8245453.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245453/SRX8245453.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245453/SRX8245453.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 20:33:52: 9000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 20:33:56: 10000000 INFO @ Sat, 15 Jan 2022 20:34:01: 11000000 BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 20:34:05: 12000000 INFO @ Sat, 15 Jan 2022 20:34:10: 13000000 INFO @ Sat, 15 Jan 2022 20:34:14: 14000000 INFO @ Sat, 15 Jan 2022 20:34:16: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 20:34:16: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 20:34:16: #1 total tags in treatment: 4465942 INFO @ Sat, 15 Jan 2022 20:34:16: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 20:34:16: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 20:34:16: #1 tags after filtering in treatment: 2753437 INFO @ Sat, 15 Jan 2022 20:34:16: #1 Redundant rate of treatment: 0.38 INFO @ Sat, 15 Jan 2022 20:34:16: #1 finished! INFO @ Sat, 15 Jan 2022 20:34:16: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 20:34:16: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 20:34:16: #2 number of paired peaks: 41 WARNING @ Sat, 15 Jan 2022 20:34:16: Too few paired peaks (41) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 20:34:16: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8245453/SRX8245453.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245453/SRX8245453.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245453/SRX8245453.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245453/SRX8245453.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling