Job ID = 14520965 SRX = SRX8245451 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 31770379 spots for SRR11684662/SRR11684662.sra Written 31770379 spots for SRR11684662/SRR11684662.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:54:24 31770379 reads; of these: 31770379 (100.00%) were paired; of these: 12944800 (40.74%) aligned concordantly 0 times 14209446 (44.73%) aligned concordantly exactly 1 time 4616133 (14.53%) aligned concordantly >1 times ---- 12944800 pairs aligned concordantly 0 times; of these: 137368 (1.06%) aligned discordantly 1 time ---- 12807432 pairs aligned 0 times concordantly or discordantly; of these: 25614864 mates make up the pairs; of these: 14832870 (57.91%) aligned 0 times 8018917 (31.31%) aligned exactly 1 time 2763077 (10.79%) aligned >1 times 76.66% overall alignment rate Time searching: 00:54:24 Overall time: 00:54:24 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 20 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 1695948 / 18950956 = 0.0895 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 21:29:47: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8245451/SRX8245451.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8245451/SRX8245451.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8245451/SRX8245451.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8245451/SRX8245451.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 21:29:47: #1 read tag files... INFO @ Sat, 15 Jan 2022 21:29:47: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 21:29:53: 1000000 INFO @ Sat, 15 Jan 2022 21:29:59: 2000000 INFO @ Sat, 15 Jan 2022 21:30:05: 3000000 INFO @ Sat, 15 Jan 2022 21:30:11: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 21:30:16: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8245451/SRX8245451.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8245451/SRX8245451.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8245451/SRX8245451.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8245451/SRX8245451.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 21:30:16: #1 read tag files... INFO @ Sat, 15 Jan 2022 21:30:16: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 21:30:17: 5000000 INFO @ Sat, 15 Jan 2022 21:30:22: 1000000 INFO @ Sat, 15 Jan 2022 21:30:23: 6000000 INFO @ Sat, 15 Jan 2022 21:30:29: 2000000 INFO @ Sat, 15 Jan 2022 21:30:29: 7000000 INFO @ Sat, 15 Jan 2022 21:30:35: 3000000 INFO @ Sat, 15 Jan 2022 21:30:35: 8000000 INFO @ Sat, 15 Jan 2022 21:30:41: 4000000 INFO @ Sat, 15 Jan 2022 21:30:41: 9000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 21:30:46: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8245451/SRX8245451.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8245451/SRX8245451.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8245451/SRX8245451.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8245451/SRX8245451.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 21:30:46: #1 read tag files... INFO @ Sat, 15 Jan 2022 21:30:46: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 21:30:47: 5000000 INFO @ Sat, 15 Jan 2022 21:30:47: 10000000 INFO @ Sat, 15 Jan 2022 21:30:52: 1000000 INFO @ Sat, 15 Jan 2022 21:30:53: 6000000 INFO @ Sat, 15 Jan 2022 21:30:54: 11000000 INFO @ Sat, 15 Jan 2022 21:30:59: 2000000 INFO @ Sat, 15 Jan 2022 21:30:59: 7000000 INFO @ Sat, 15 Jan 2022 21:31:00: 12000000 INFO @ Sat, 15 Jan 2022 21:31:05: 3000000 INFO @ Sat, 15 Jan 2022 21:31:06: 8000000 INFO @ Sat, 15 Jan 2022 21:31:07: 13000000 INFO @ Sat, 15 Jan 2022 21:31:11: 4000000 INFO @ Sat, 15 Jan 2022 21:31:12: 9000000 INFO @ Sat, 15 Jan 2022 21:31:14: 14000000 INFO @ Sat, 15 Jan 2022 21:31:17: 5000000 INFO @ Sat, 15 Jan 2022 21:31:18: 10000000 INFO @ Sat, 15 Jan 2022 21:31:20: 15000000 INFO @ Sat, 15 Jan 2022 21:31:24: 11000000 INFO @ Sat, 15 Jan 2022 21:31:24: 6000000 INFO @ Sat, 15 Jan 2022 21:31:27: 16000000 INFO @ Sat, 15 Jan 2022 21:31:31: 12000000 INFO @ Sat, 15 Jan 2022 21:31:32: 7000000 INFO @ Sat, 15 Jan 2022 21:31:33: 17000000 INFO @ Sat, 15 Jan 2022 21:31:37: 13000000 INFO @ Sat, 15 Jan 2022 21:31:38: 8000000 INFO @ Sat, 15 Jan 2022 21:31:40: 18000000 INFO @ Sat, 15 Jan 2022 21:31:43: 14000000 INFO @ Sat, 15 Jan 2022 21:31:44: 9000000 INFO @ Sat, 15 Jan 2022 21:31:46: 19000000 INFO @ Sat, 15 Jan 2022 21:31:50: 15000000 INFO @ Sat, 15 Jan 2022 21:31:50: 10000000 INFO @ Sat, 15 Jan 2022 21:31:52: 20000000 INFO @ Sat, 15 Jan 2022 21:31:56: 16000000 INFO @ Sat, 15 Jan 2022 21:31:56: 11000000 INFO @ Sat, 15 Jan 2022 21:31:59: 21000000 INFO @ Sat, 15 Jan 2022 21:32:02: 17000000 INFO @ Sat, 15 Jan 2022 21:32:03: 12000000 INFO @ Sat, 15 Jan 2022 21:32:06: 22000000 INFO @ Sat, 15 Jan 2022 21:32:08: 18000000 INFO @ Sat, 15 Jan 2022 21:32:09: 13000000 INFO @ Sat, 15 Jan 2022 21:32:13: 23000000 INFO @ Sat, 15 Jan 2022 21:32:15: 19000000 INFO @ Sat, 15 Jan 2022 21:32:15: 14000000 INFO @ Sat, 15 Jan 2022 21:32:19: 24000000 INFO @ Sat, 15 Jan 2022 21:32:21: 20000000 INFO @ Sat, 15 Jan 2022 21:32:21: 15000000 INFO @ Sat, 15 Jan 2022 21:32:26: 25000000 INFO @ Sat, 15 Jan 2022 21:32:27: 21000000 INFO @ Sat, 15 Jan 2022 21:32:28: 16000000 INFO @ Sat, 15 Jan 2022 21:32:33: 26000000 INFO @ Sat, 15 Jan 2022 21:32:34: 22000000 INFO @ Sat, 15 Jan 2022 21:32:34: 17000000 INFO @ Sat, 15 Jan 2022 21:32:39: 27000000 INFO @ Sat, 15 Jan 2022 21:32:40: 18000000 INFO @ Sat, 15 Jan 2022 21:32:41: 23000000 INFO @ Sat, 15 Jan 2022 21:32:46: 28000000 INFO @ Sat, 15 Jan 2022 21:32:47: 24000000 INFO @ Sat, 15 Jan 2022 21:32:47: 19000000 INFO @ Sat, 15 Jan 2022 21:32:53: 29000000 INFO @ Sat, 15 Jan 2022 21:32:53: 20000000 INFO @ Sat, 15 Jan 2022 21:32:54: 25000000 INFO @ Sat, 15 Jan 2022 21:33:00: 21000000 INFO @ Sat, 15 Jan 2022 21:33:00: 30000000 INFO @ Sat, 15 Jan 2022 21:33:00: 26000000 INFO @ Sat, 15 Jan 2022 21:33:06: 22000000 INFO @ Sat, 15 Jan 2022 21:33:06: 31000000 INFO @ Sat, 15 Jan 2022 21:33:07: 27000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 21:33:12: 23000000 INFO @ Sat, 15 Jan 2022 21:33:13: 32000000 INFO @ Sat, 15 Jan 2022 21:33:14: 28000000 INFO @ Sat, 15 Jan 2022 21:33:18: 24000000 INFO @ Sat, 15 Jan 2022 21:33:19: 33000000 INFO @ Sat, 15 Jan 2022 21:33:20: 29000000 BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 21:33:24: 25000000 INFO @ Sat, 15 Jan 2022 21:33:26: 34000000 INFO @ Sat, 15 Jan 2022 21:33:27: 30000000 INFO @ Sat, 15 Jan 2022 21:33:30: 26000000 INFO @ Sat, 15 Jan 2022 21:33:32: 35000000 INFO @ Sat, 15 Jan 2022 21:33:33: 31000000 INFO @ Sat, 15 Jan 2022 21:33:36: 27000000 INFO @ Sat, 15 Jan 2022 21:33:38: 36000000 INFO @ Sat, 15 Jan 2022 21:33:39: 32000000 INFO @ Sat, 15 Jan 2022 21:33:41: 28000000 INFO @ Sat, 15 Jan 2022 21:33:45: 37000000 INFO @ Sat, 15 Jan 2022 21:33:46: 33000000 INFO @ Sat, 15 Jan 2022 21:33:47: 29000000 INFO @ Sat, 15 Jan 2022 21:33:51: 38000000 INFO @ Sat, 15 Jan 2022 21:33:52: 34000000 INFO @ Sat, 15 Jan 2022 21:33:53: 30000000 INFO @ Sat, 15 Jan 2022 21:33:57: 39000000 INFO @ Sat, 15 Jan 2022 21:33:58: 35000000 INFO @ Sat, 15 Jan 2022 21:33:59: 31000000 INFO @ Sat, 15 Jan 2022 21:34:03: 40000000 INFO @ Sat, 15 Jan 2022 21:34:04: 36000000 INFO @ Sat, 15 Jan 2022 21:34:05: 32000000 INFO @ Sat, 15 Jan 2022 21:34:10: 41000000 INFO @ Sat, 15 Jan 2022 21:34:11: 37000000 INFO @ Sat, 15 Jan 2022 21:34:11: 33000000 INFO @ Sat, 15 Jan 2022 21:34:16: 42000000 INFO @ Sat, 15 Jan 2022 21:34:17: 34000000 INFO @ Sat, 15 Jan 2022 21:34:17: 38000000 INFO @ Sat, 15 Jan 2022 21:34:22: 35000000 INFO @ Sat, 15 Jan 2022 21:34:23: 43000000 INFO @ Sat, 15 Jan 2022 21:34:23: 39000000 INFO @ Sat, 15 Jan 2022 21:34:28: 36000000 INFO @ Sat, 15 Jan 2022 21:34:29: 44000000 INFO @ Sat, 15 Jan 2022 21:34:30: 40000000 INFO @ Sat, 15 Jan 2022 21:34:34: 37000000 INFO @ Sat, 15 Jan 2022 21:34:36: 45000000 INFO @ Sat, 15 Jan 2022 21:34:36: 41000000 INFO @ Sat, 15 Jan 2022 21:34:38: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 21:34:38: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 21:34:38: #1 total tags in treatment: 17131750 INFO @ Sat, 15 Jan 2022 21:34:38: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 21:34:38: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 21:34:38: #1 tags after filtering in treatment: 4042731 INFO @ Sat, 15 Jan 2022 21:34:38: #1 Redundant rate of treatment: 0.76 INFO @ Sat, 15 Jan 2022 21:34:38: #1 finished! INFO @ Sat, 15 Jan 2022 21:34:38: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 21:34:38: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 21:34:38: #2 number of paired peaks: 14 WARNING @ Sat, 15 Jan 2022 21:34:38: Too few paired peaks (14) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 21:34:38: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8245451/SRX8245451.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245451/SRX8245451.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245451/SRX8245451.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245451/SRX8245451.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 21:34:40: 38000000 INFO @ Sat, 15 Jan 2022 21:34:42: 42000000 INFO @ Sat, 15 Jan 2022 21:34:45: 39000000 INFO @ Sat, 15 Jan 2022 21:34:48: 43000000 INFO @ Sat, 15 Jan 2022 21:34:51: 40000000 INFO @ Sat, 15 Jan 2022 21:34:54: 44000000 INFO @ Sat, 15 Jan 2022 21:34:57: 41000000 INFO @ Sat, 15 Jan 2022 21:35:00: 45000000 INFO @ Sat, 15 Jan 2022 21:35:02: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 21:35:02: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 21:35:02: #1 total tags in treatment: 17131750 INFO @ Sat, 15 Jan 2022 21:35:02: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 21:35:02: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 21:35:03: #1 tags after filtering in treatment: 4042731 INFO @ Sat, 15 Jan 2022 21:35:03: #1 Redundant rate of treatment: 0.76 INFO @ Sat, 15 Jan 2022 21:35:03: #1 finished! INFO @ Sat, 15 Jan 2022 21:35:03: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 21:35:03: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 21:35:03: 42000000 INFO @ Sat, 15 Jan 2022 21:35:03: #2 number of paired peaks: 14 WARNING @ Sat, 15 Jan 2022 21:35:03: Too few paired peaks (14) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 21:35:03: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8245451/SRX8245451.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245451/SRX8245451.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245451/SRX8245451.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245451/SRX8245451.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 21:35:09: 43000000 INFO @ Sat, 15 Jan 2022 21:35:15: 44000000 INFO @ Sat, 15 Jan 2022 21:35:21: 45000000 INFO @ Sat, 15 Jan 2022 21:35:23: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 21:35:23: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 21:35:23: #1 total tags in treatment: 17131750 INFO @ Sat, 15 Jan 2022 21:35:23: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 21:35:23: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 21:35:23: #1 tags after filtering in treatment: 4042731 INFO @ Sat, 15 Jan 2022 21:35:23: #1 Redundant rate of treatment: 0.76 INFO @ Sat, 15 Jan 2022 21:35:23: #1 finished! INFO @ Sat, 15 Jan 2022 21:35:23: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 21:35:23: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 21:35:24: #2 number of paired peaks: 14 WARNING @ Sat, 15 Jan 2022 21:35:24: Too few paired peaks (14) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 21:35:24: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8245451/SRX8245451.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245451/SRX8245451.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245451/SRX8245451.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245451/SRX8245451.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling