Job ID = 14520935 SRX = SRX8245448 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 20835318 spots for SRR11684659/SRR11684659.sra Written 20835318 spots for SRR11684659/SRR11684659.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:20:44 20835318 reads; of these: 20835318 (100.00%) were paired; of these: 11282412 (54.15%) aligned concordantly 0 times 6562806 (31.50%) aligned concordantly exactly 1 time 2990100 (14.35%) aligned concordantly >1 times ---- 11282412 pairs aligned concordantly 0 times; of these: 218301 (1.93%) aligned discordantly 1 time ---- 11064111 pairs aligned 0 times concordantly or discordantly; of these: 22128222 mates make up the pairs; of these: 15920124 (71.94%) aligned 0 times 4304183 (19.45%) aligned exactly 1 time 1903915 (8.60%) aligned >1 times 61.80% overall alignment rate Time searching: 00:20:44 Overall time: 00:20:44 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 12 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 1460870 / 9703140 = 0.1506 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 20:40:39: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8245448/SRX8245448.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8245448/SRX8245448.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8245448/SRX8245448.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8245448/SRX8245448.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 20:40:39: #1 read tag files... INFO @ Sat, 15 Jan 2022 20:40:39: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 20:40:44: 1000000 INFO @ Sat, 15 Jan 2022 20:40:49: 2000000 INFO @ Sat, 15 Jan 2022 20:40:54: 3000000 INFO @ Sat, 15 Jan 2022 20:40:59: 4000000 INFO @ Sat, 15 Jan 2022 20:41:05: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 20:41:09: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8245448/SRX8245448.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8245448/SRX8245448.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8245448/SRX8245448.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8245448/SRX8245448.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 20:41:09: #1 read tag files... INFO @ Sat, 15 Jan 2022 20:41:09: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 20:41:10: 6000000 INFO @ Sat, 15 Jan 2022 20:41:14: 1000000 INFO @ Sat, 15 Jan 2022 20:41:15: 7000000 INFO @ Sat, 15 Jan 2022 20:41:20: 2000000 INFO @ Sat, 15 Jan 2022 20:41:20: 8000000 INFO @ Sat, 15 Jan 2022 20:41:26: 9000000 INFO @ Sat, 15 Jan 2022 20:41:26: 3000000 INFO @ Sat, 15 Jan 2022 20:41:31: 10000000 INFO @ Sat, 15 Jan 2022 20:41:32: 4000000 INFO @ Sat, 15 Jan 2022 20:41:36: 11000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 20:41:37: 5000000 INFO @ Sat, 15 Jan 2022 20:41:39: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8245448/SRX8245448.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8245448/SRX8245448.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8245448/SRX8245448.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8245448/SRX8245448.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 20:41:39: #1 read tag files... INFO @ Sat, 15 Jan 2022 20:41:39: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 20:41:42: 12000000 INFO @ Sat, 15 Jan 2022 20:41:43: 6000000 INFO @ Sat, 15 Jan 2022 20:41:44: 1000000 INFO @ Sat, 15 Jan 2022 20:41:48: 13000000 INFO @ Sat, 15 Jan 2022 20:41:48: 7000000 INFO @ Sat, 15 Jan 2022 20:41:49: 2000000 INFO @ Sat, 15 Jan 2022 20:41:53: 14000000 INFO @ Sat, 15 Jan 2022 20:41:54: 3000000 INFO @ Sat, 15 Jan 2022 20:41:54: 8000000 INFO @ Sat, 15 Jan 2022 20:41:59: 4000000 INFO @ Sat, 15 Jan 2022 20:41:59: 15000000 INFO @ Sat, 15 Jan 2022 20:42:00: 9000000 INFO @ Sat, 15 Jan 2022 20:42:04: 5000000 INFO @ Sat, 15 Jan 2022 20:42:04: 16000000 INFO @ Sat, 15 Jan 2022 20:42:06: 10000000 INFO @ Sat, 15 Jan 2022 20:42:09: 6000000 INFO @ Sat, 15 Jan 2022 20:42:10: 17000000 INFO @ Sat, 15 Jan 2022 20:42:12: 11000000 INFO @ Sat, 15 Jan 2022 20:42:13: 7000000 INFO @ Sat, 15 Jan 2022 20:42:15: 18000000 INFO @ Sat, 15 Jan 2022 20:42:17: 12000000 INFO @ Sat, 15 Jan 2022 20:42:18: 8000000 INFO @ Sat, 15 Jan 2022 20:42:21: 19000000 INFO @ Sat, 15 Jan 2022 20:42:23: 9000000 INFO @ Sat, 15 Jan 2022 20:42:23: 13000000 INFO @ Sat, 15 Jan 2022 20:42:27: 20000000 INFO @ Sat, 15 Jan 2022 20:42:28: 10000000 INFO @ Sat, 15 Jan 2022 20:42:29: 14000000 INFO @ Sat, 15 Jan 2022 20:42:32: 21000000 INFO @ Sat, 15 Jan 2022 20:42:33: 11000000 INFO @ Sat, 15 Jan 2022 20:42:35: 15000000 INFO @ Sat, 15 Jan 2022 20:42:38: 22000000 INFO @ Sat, 15 Jan 2022 20:42:38: 12000000 INFO @ Sat, 15 Jan 2022 20:42:40: 16000000 INFO @ Sat, 15 Jan 2022 20:42:42: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 20:42:42: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 20:42:42: #1 total tags in treatment: 8101909 INFO @ Sat, 15 Jan 2022 20:42:42: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 20:42:42: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 20:42:43: #1 tags after filtering in treatment: 4797706 INFO @ Sat, 15 Jan 2022 20:42:43: #1 Redundant rate of treatment: 0.41 INFO @ Sat, 15 Jan 2022 20:42:43: #1 finished! INFO @ Sat, 15 Jan 2022 20:42:43: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 20:42:43: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 20:42:43: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 20:42:43: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 20:42:43: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8245448/SRX8245448.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245448/SRX8245448.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245448/SRX8245448.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245448/SRX8245448.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 20:42:43: 13000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 20:42:46: 17000000 INFO @ Sat, 15 Jan 2022 20:42:48: 14000000 INFO @ Sat, 15 Jan 2022 20:42:52: 18000000 INFO @ Sat, 15 Jan 2022 20:42:53: 15000000 INFO @ Sat, 15 Jan 2022 20:42:58: 19000000 BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 20:42:59: 16000000 INFO @ Sat, 15 Jan 2022 20:43:03: 20000000 INFO @ Sat, 15 Jan 2022 20:43:04: 17000000 INFO @ Sat, 15 Jan 2022 20:43:09: 18000000 INFO @ Sat, 15 Jan 2022 20:43:09: 21000000 INFO @ Sat, 15 Jan 2022 20:43:14: 19000000 INFO @ Sat, 15 Jan 2022 20:43:15: 22000000 INFO @ Sat, 15 Jan 2022 20:43:19: 20000000 INFO @ Sat, 15 Jan 2022 20:43:20: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 20:43:20: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 20:43:20: #1 total tags in treatment: 8101909 INFO @ Sat, 15 Jan 2022 20:43:20: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 20:43:20: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 20:43:20: #1 tags after filtering in treatment: 4797706 INFO @ Sat, 15 Jan 2022 20:43:20: #1 Redundant rate of treatment: 0.41 INFO @ Sat, 15 Jan 2022 20:43:20: #1 finished! INFO @ Sat, 15 Jan 2022 20:43:20: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 20:43:20: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 20:43:20: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 20:43:20: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 20:43:20: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8245448/SRX8245448.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245448/SRX8245448.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245448/SRX8245448.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245448/SRX8245448.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 20:43:24: 21000000 INFO @ Sat, 15 Jan 2022 20:43:28: 22000000 INFO @ Sat, 15 Jan 2022 20:43:32: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 20:43:32: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 20:43:32: #1 total tags in treatment: 8101909 INFO @ Sat, 15 Jan 2022 20:43:32: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 20:43:32: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 20:43:32: #1 tags after filtering in treatment: 4797706 INFO @ Sat, 15 Jan 2022 20:43:32: #1 Redundant rate of treatment: 0.41 INFO @ Sat, 15 Jan 2022 20:43:32: #1 finished! INFO @ Sat, 15 Jan 2022 20:43:32: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 20:43:32: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 20:43:33: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 20:43:33: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 20:43:33: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8245448/SRX8245448.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245448/SRX8245448.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245448/SRX8245448.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245448/SRX8245448.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling